EP0599651A3 - Bioréacteur pour la culture de cellules en perfusion. - Google Patents

Bioréacteur pour la culture de cellules en perfusion. Download PDF

Info

Publication number
EP0599651A3
EP0599651A3 EP93309432A EP93309432A EP0599651A3 EP 0599651 A3 EP0599651 A3 EP 0599651A3 EP 93309432 A EP93309432 A EP 93309432A EP 93309432 A EP93309432 A EP 93309432A EP 0599651 A3 EP0599651 A3 EP 0599651A3
Authority
EP
European Patent Office
Prior art keywords
bioreactor
cells
perfusion culture
perfusion
culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP93309432A
Other languages
German (de)
English (en)
Other versions
EP0599651A2 (fr
Inventor
Gerald Andre
Claude Chavarie
Christian Knaack
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ecole Polytechnique de Montreal
National Research Council of Canada
Original Assignee
National Research Council of Canada
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Research Council of Canada filed Critical National Research Council of Canada
Publication of EP0599651A2 publication Critical patent/EP0599651A2/fr
Publication of EP0599651A3 publication Critical patent/EP0599651A3/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/0018—Separation of suspended solid particles from liquids by sedimentation provided with a pump mounted in or on a settling tank
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/0039—Settling tanks provided with contact surfaces, e.g. baffles, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/0039—Settling tanks provided with contact surfaces, e.g. baffles, particles
    • B01D21/0045—Plurality of essentially parallel plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/02—Settling tanks with single outlets for the separated liquid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/24—Draft tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2221/00—Applications of separation devices
    • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP93309432A 1992-11-25 1993-11-25 Bioréacteur pour la culture de cellules en perfusion. Withdrawn EP0599651A3 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/981,490 US5320963A (en) 1992-11-25 1992-11-25 Bioreactor for the perfusion culture of cells
US981490 1992-11-25

Publications (2)

Publication Number Publication Date
EP0599651A2 EP0599651A2 (fr) 1994-06-01
EP0599651A3 true EP0599651A3 (fr) 1996-01-24

Family

ID=25528404

Family Applications (1)

Application Number Title Priority Date Filing Date
EP93309432A Withdrawn EP0599651A3 (fr) 1992-11-25 1993-11-25 Bioréacteur pour la culture de cellules en perfusion.

Country Status (3)

Country Link
US (1) US5320963A (fr)
EP (1) EP0599651A3 (fr)
CA (1) CA2109897A1 (fr)

Families Citing this family (45)

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AU689998C (en) * 1995-01-13 2002-08-08 Enviro Research Pty Ltd Apparatus for biomass production
AUPN060095A0 (en) * 1995-01-13 1995-02-09 Enviro Research Pty Ltd Apparatus for biomass production
US5811259A (en) * 1997-07-29 1998-09-22 Ecomat, Inc. Biochemical reactor
EP1117762A4 (fr) * 1998-09-29 2004-02-25 Gamida Cell Ltd Procedes de regulation de la proliferation et de la differentiation de cellules souches et precurseurs
CA2877233A1 (fr) 1999-01-19 2000-07-27 Lola M. Reid Progeniteurs de foie humain
US6632658B1 (en) * 2000-08-18 2003-10-14 Levitronix Llc Bioreactor and method for fluidly suspending a product
US6864084B2 (en) * 2000-08-18 2005-03-08 Levitronix Llc Bioreactor and method for fluidly suspending a product
IL152904A0 (en) * 2002-01-24 2003-06-24 Gamida Cell Ltd Utilization of retinoid and vitamin d receptor antagonists for expansion of renewable stem cell populations
WO2003062404A1 (fr) * 2002-01-25 2003-07-31 Gamida-Cell Ltd. Methodes de developpement de cellules souches et de cellules precurseurs, populations de cellules multipliees obtenues a l'aide de ces methodes
JP2005520511A (ja) * 2002-03-18 2005-07-14 ガミダ セル リミテッド エクスビボ拡大培養された幹細胞における分化を誘導する方法
US20050054097A1 (en) * 2002-11-17 2005-03-10 Tony Peled EX-VIVO expansion of hematopoietic system cell populations in mononuclear cell cultures
US20050054103A1 (en) * 2003-03-07 2005-03-10 Tony Peled Expansion of renewable stem cell populations using modulators of PI 3-kinase
US20050233448A1 (en) * 2003-06-18 2005-10-20 Oh Steve K W Materials and methods to produce stem cells
US20050032208A1 (en) * 2003-06-18 2005-02-10 Oh Steve Kah Weng Materials and methods to produce stem cells
IL161903A0 (en) * 2003-07-17 2005-11-20 Gamida Cell Ltd Ex vivo progenitor and stem cell expansion for usein the treatment of disease of endodermally- deri ved organs
WO2006030442A2 (fr) * 2004-09-16 2006-03-23 Gamida-Cell Ltd. Methodes de culture ex vivo de cellules souches et de precurseur par co-culture avec des cellules mesenchymales
JP2008543307A (ja) * 2005-06-15 2008-12-04 アンプロテイン コーポレイション 懸濁培養用容器
US8846393B2 (en) 2005-11-29 2014-09-30 Gamida-Cell Ltd. Methods of improving stem cell homing and engraftment
US9057044B2 (en) 2006-08-30 2015-06-16 Meir Israelowitz Laminar flow reactor
DE102007020671A1 (de) * 2007-05-01 2008-11-06 Justus-Liebig-Universität Giessen Verfahren und Vorrichtung zum kontaktfreien Materialwachstum in einer strömenden Lösung (FLow suspended solution growth (FSSG))
US20100076380A1 (en) * 2008-06-16 2010-03-25 Amprotein Corporation Bioreactors
DE102008029307A1 (de) * 2008-06-20 2009-12-24 Bayer Technology Services Gmbh Verfahren und Vorrichtung zur Rückhaltung und Rückführung von Zellen
CN108342353B (zh) 2008-11-04 2022-01-11 韦尔赛特公司 干细胞聚集体悬浮组合物及其分化方法
EP4036207A1 (fr) * 2011-01-17 2022-08-03 F. Hoffmann-La Roche AG Utilisation d'un appareil de separation dans les chaînes d'ensemencement
FR2971255B1 (fr) 2011-02-04 2015-03-27 Centre Nat Rech Scient Bioreacteur pour la culture cellulaire sur substrat tridimensionnel
US8365462B2 (en) * 2011-05-31 2013-02-05 Heliae Development, Llc V-Trough photobioreactor systems
USD682637S1 (en) 2011-06-10 2013-05-21 Heliae Development, Llc Aquaculture vessel
USD679965S1 (en) 2011-06-10 2013-04-16 Heliae Development, Llc Aquaculture vessel
WO2013095300A1 (fr) * 2011-12-19 2013-06-27 Nanyang Technological University Bioréacteur
CN104204192A (zh) 2012-02-13 2014-12-10 加米达细胞有限公司 间充质干细胞调理的基质及其产生和使用方法
BR112014020462B1 (pt) 2012-02-20 2020-11-10 Bayer Aktiengesellschaft separador unidirecional para retenção e recirculação de células
US9567569B2 (en) 2012-07-23 2017-02-14 Gamida Cell Ltd. Methods of culturing and expanding mesenchymal stem cells
US9175266B2 (en) 2012-07-23 2015-11-03 Gamida Cell Ltd. Enhancement of natural killer (NK) cell proliferation and activity
AU2013248265B2 (en) 2012-11-08 2018-11-01 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
US9550972B2 (en) * 2014-09-29 2017-01-24 General Electric Company Devices, systems and methods for automated cell culturing
US9944894B2 (en) * 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
BR112017023624A2 (pt) * 2015-05-29 2018-07-17 Glycotope Gmbh método de cultivo em pequena escala para células em suspensão
JP2017029095A (ja) * 2015-08-04 2017-02-09 オリンパス株式会社 細胞培養装置
AU2017316545B2 (en) * 2016-08-21 2022-12-08 Adva Biotechnology Ltd. Bioreactor and methods of use thereof
CN106834088A (zh) * 2017-03-29 2017-06-13 江苏丰泽生物工程设备制造有限公司 一种用于生物反应器中的细胞收集器
US11434461B2 (en) * 2018-03-20 2022-09-06 Keck Graduate Institute Of Applied Life Sciences Airlift perfusion bioreactor for the culture of cells
CN112020385B (zh) 2018-04-18 2023-01-06 苏德新生物制药公司 颗粒沉降装置
US20210155885A1 (en) * 2018-04-25 2021-05-27 Global Life Sciences Solutions Usa Llc Bioreactors
US11447729B2 (en) * 2018-12-21 2022-09-20 Global Life Sciences Solutions Usa Llc Apparatus and method for bioprocessing
WO2021188820A1 (fr) * 2020-03-19 2021-09-23 Sudhin Biopharma Dispositifs de sédimentation de particules

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2902753A1 (de) * 1979-01-25 1980-08-07 Metallgesellschaft Ag Verfahren und vorrichtung zum ausfuehren biotechnischer reaktionen
WO1986007604A1 (fr) * 1985-06-20 1986-12-31 Celltech Limited Fermenteur
US4814278A (en) * 1985-02-05 1989-03-21 Teijin Limited Culture apparatus and method
WO1991006627A1 (fr) * 1989-11-03 1991-05-16 Massachusetts Institute Of Technology Separateur a cones emboites

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US2083348A (en) * 1934-09-24 1937-06-08 Scholler Heinrich Apparatus for the production of micro-organisms and for the fermentation of solutions
US3635346A (en) * 1970-02-24 1972-01-18 Envirotech Corp Solids separator
US4335215A (en) * 1980-08-27 1982-06-15 Monsanto Company Method of growing anchorage-dependent cells
JPS62265A (ja) * 1985-02-05 1987-01-06 Teijin Ltd 細胞培養装置および方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2902753A1 (de) * 1979-01-25 1980-08-07 Metallgesellschaft Ag Verfahren und vorrichtung zum ausfuehren biotechnischer reaktionen
US4814278A (en) * 1985-02-05 1989-03-21 Teijin Limited Culture apparatus and method
WO1986007604A1 (fr) * 1985-06-20 1986-12-31 Celltech Limited Fermenteur
WO1991006627A1 (fr) * 1989-11-03 1991-05-16 Massachusetts Institute Of Technology Separateur a cones emboites

Also Published As

Publication number Publication date
CA2109897A1 (fr) 1994-05-26
US5320963A (en) 1994-06-14
EP0599651A2 (fr) 1994-06-01

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Effective date: 19960725