EP0530186A1 - Porte-objets pour preparations a teneur en eau, par exemple, de sang. - Google Patents

Porte-objets pour preparations a teneur en eau, par exemple, de sang.

Info

Publication number
EP0530186A1
EP0530186A1 EP90916289A EP90916289A EP0530186A1 EP 0530186 A1 EP0530186 A1 EP 0530186A1 EP 90916289 A EP90916289 A EP 90916289A EP 90916289 A EP90916289 A EP 90916289A EP 0530186 A1 EP0530186 A1 EP 0530186A1
Authority
EP
European Patent Office
Prior art keywords
plate
plates
frame
slide according
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP90916289A
Other languages
German (de)
English (en)
Other versions
EP0530186B1 (fr
Inventor
Rainer Dylla
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP0530186A1 publication Critical patent/EP0530186A1/fr
Application granted granted Critical
Publication of EP0530186B1 publication Critical patent/EP0530186B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures

Definitions

  • the invention relates to a slide (also called specimen slide or spot plate) for wet or moist preparations, for.
  • a slide also called specimen slide or spot plate
  • chemical or biological preparations such as blood, consisting of a plate of preferably transparent material, in particular of transparent plastic, with a plurality of receptacles for the preparations carried by the surface, a second plate which can be placed on the first plate being provided, the surface of which is designed such that it forms a flat or slightly curved, areally extending gap space with a gap width such that the volume of the preparation essentially fills the volume of the gap space with the surface of the receptacles of the first plate.
  • DD 224 410 A1 shows a sample holder which essentially corresponds to the arrangement described at the beginning.
  • this publication emphasizes the inexpensive producibility and the simple and safe handling in the laboratory. According to this document, this inexpensive producibility is essentially emphasized by the identical surface shape of both the upper and the lower plate. In the middle The point of this publication is the border of the sample holder, which is designed in such a way that it causes mutual alignment in such a position that the elevations and depressions of the plates facing each other are exactly aligned with one another.
  • the sample carrier also comprises two plates on which bodies for sealing the sample-bearing areas of the two plates and for their horizontal position fixing and vertical distance limit, these bodies are made of elastic material in order to be able to move the plates vertically within a certain distance.
  • a disadvantage is the inaccurate alignment due to the grooves and their complicated manufacture.
  • DD 224 410 A1 in all known technical solutions the sample carrier is formed from two non-identical plates, which according to this DD 224 410 A1 has the disadvantage that two different plates have to be produced, which is particularly the case with an injection molding implementation would cause additional costs for the second tool required. Even when using the sample holder in the laboratory, the handling of two different plates has a disadvantageous effect.
  • the material from which the panels are to be made is plexiglass according to DD 224 410 A1.
  • the offset arrangement enables the setting and adherence to a more exact, reproducible sample thickness and the production of optically transparent samples and sample carriers, which enables immediate observation and detection of the reactions between the sample and the test medium and their immediate photometric evaluation without the interposition of filter paper, as is the case was necessary with even older state of the art.
  • the samples are applied to the designated areas of one plate and the test agents are placed on the remote areas of the other plate, the plates are brought together with their surfaces carrying the reagents and the samples with the test agent by repeated slight Changing the distance between the two plates mixed by hand. The short distance also provides protection against rapid evaporation of the samples.
  • the plates can also be in the form of a film, as a result of which spacers could then be omitted because the plates are flexible enough so that the areas carrying the samples can be moved against one another.
  • the evaluation can be carried out subjectively by visual observation or objectively with analysis measuring devices or analysis machines.
  • EP 0018435 B1 deals with a device for performing microanalyses and describes in this connection various prior art in detail.
  • the delimitation of the sample areas takes place here by the application of water-repellent substances in such a way that a certain number of circular areas remain free. Mixing is also achieved here by changing the distance between the plates.
  • the plate movement is carried out by a magnet and by springs, the effect of the magnet are opposite.
  • DE 31 07 964 A1 originates from the same inventor as this EP 0018435 B1 and in this respect represents a further development of EP 0018435 B1.
  • Figure 9 of DE 31 07 964 A1 shows frames in order to "microscope" on d: 6sen7 sample plates. The frames are essentially used for fixation when optically examining the samples.
  • OE-PS 17 08 98 deals with a shaking frame and which can be regarded as the forerunner of microtiter technology.
  • DD 96 784 describes a method and a device for measuring enzymatic reactions using plates in which a greater number of depressions are provided for receiving test liquids, these areas being separated from one another by grooves in order to prevent the test liquid from overflowing from one test area to the other test area.
  • test methods described which (advantageously) manage with very small amounts of preparation and test agent and therefore with a given amount of test liquid and amount of preparation (such as blood) enables a significantly higher number of tests than previous test methods allowed, on the other hand the danger is avoided relatively large that only small amounts of foreign matter can deliberately or unintentionally falsify the test result.
  • the test result of a subsequent test could be falsified by residues of previous preparation and test liquids or test materials that could not be removed cleanly enough . It is also possible with relatively little effort to change the test result through deliberate manipulation.
  • test materials cannot be released into the free environment again, for example by washing, and can lead to contamination or even infection there.
  • the plate (s) and / or a frame carrying the plate (s) and / or at least one of the receiving surfaces have a locking and / or marking device , which takes effect when the plate (s) are used for the first time and thereby forms a lock and / or warning indicator against further use.
  • the receiving surface or at least part of it has a coating which discolors when used and thereby provides a warning display.
  • the analysis layer itself can represent such a coating.
  • an additional coating could be provided on the receiving surface in addition to this analysis coating, which results in this discoloration.
  • the receiving surface can form a capillary-like depression or a gap for marking the use that has taken place, in that test or preparation material penetrates into this gap and settles there and cannot be removed there either.
  • the gap in the translucent material changes its appearance and thereby marks the use.
  • the plates can have locking devices that have a limited movement of the plates perpendicular to the plane of the plate against spring force or the like enables each other • to change the gap volume, but on the other hand does not allow a complete separation of the plates from each other.
  • Mixing processes can be carried out in a manner known per se with the aid of the gap volume change. Mixing operations of this type are useful because, owing to the small amounts of test material, such as blood serum and test agents, these can only be insufficiently mixed using conventional methods.
  • the novel microtiter method in which only a relatively thin film forms between the receiving surfaces of the two plates and in which this film is contracted and pushed apart again by changing the distance between the plates, allows relatively small amounts of preparation and test material to be used, especially since Material pulled apart over a relatively large area and can thus be more easily inspected optically, even if only these small quantities are involved.
  • the above-mentioned special mixing effect also results in inexpensive testing for poorly mixing preparations.
  • the movement of the plates can be generated by pressure against spring force, which spring force is provided by means of flexible approaches protruding in one piece from the plate material or by the rigidity of the plate itself, for example, as in the prior art, by means of projections emanating from the plate. or the plate levels are kept at a certain distance from one another by the plate edges, which distance can be reduced by pressure on the plates. This pressure can be exerted by an operator's hand, but also by mechanical means.
  • the plates When low, the plates has been found in making such a manner in frame inserted, that the holding device and, if necessary movement perpendicular to the plane of the plate movably aus ⁇ designed l $ j that the gap volume of space compared to the preparation volume to at least increased by a factor of 2 and / or reduced can be.
  • the receptacles can be designed in various ways, for example by means of columnar projections starting from a substrate.
  • the projections can have been created by introducing intersecting grooves into a plate. This plate would therefore be made of solid material.
  • the slide is to be designed as a disposable article, which is the aim here, it is, however, more economical in terms of material consumption to form the receptacles of bulges pressed out of a base plate made of thin plastic material.
  • These bulges can form a concave, convex or flat surface for the receptacle, while the second plate has bulges which are suitably shaped and which are also pressed out of a plate made of thin plastic material. If the bulges of the first plate are flat, it may be sufficient if the second plate has no bulges but is a simple flat plate.
  • Such plates which preferably consist of transparent plastic (one plate also consisting of opalised plastic, which sometimes has microscopic advantages), can also be designed in a simple manner so that the first and / or second plate in the region of the (respective) receptacle forms a lens-like thickening, so that, for example, even very small test quantities are still visible to the naked eye can be observed so well that an exact review of the test result is possible without z. B. to use a microscope.
  • the material that has penetrated into this capillary-like depression changes the light passage behavior in the region of the depression in such a way that it is immediately visible that the image has already been used here. If it is colored test material (such as blood), a color effect is even achieved, because e.g. B. red. Blood cells penetrate into the gap or the depression and cannot be removed therefrom, and so clearly indicate that this recording has already been has been applied and should not be used again.
  • test material such as blood
  • the slide can preferably be designed in such a way that it can be used in a (reusable) frame.
  • the specimen slide can then be made from even thinner material, since the frame can take on the mechanical loads that occur during handling (e.g. transport, centrifugation, etc.). The material consumption for the (discarded) slide is thereby further reduced.
  • the frame can be designed in such a way that it can hold several specimen slides at the same time, the specimen slide then only having as many exposures as, for example, require a normal blood test.
  • the frame in the form of a snap frame with an upper and a lower part, which two parts can be pivotally connected to one another at one edge by a joint.
  • a plurality of receptacles lying next to one another for (preferably) strip-shaped plates could then be provided.
  • These strip-shaped plates could then again each form a plurality of receiving surfaces arranged next to one another in the longitudinal direction of the strip, for preparation and / or test liquid or test agent which has been applied completely.
  • two superimposed ones can also be used strip-like plates on one edge, e.g. B. narrow edge can be connected by means of a joint and form a latching device on its opposite edge.
  • a second latching device could also be provided, so that in each case two strip-shaped plates lying one above the other form a latching device on at least two opposite edges, such as their two narrow edges.
  • the one (for example lower) strip-shaped plate could form a flag-like extension on at least one of its narrow sides in order to provide a marking or labeling surface, for example for the manufacturer's declaration of a permanently applied test agent or for user labeling Specification of patient data or preparation data.
  • test agent optionally (if no permanently applied test agent is used) introducing test agent into or onto the receptacles provided with the preparations near or to the preparation in the first plate or into or onto a corresponding receptacle of the second plate;
  • This process sequence which is known per se, is improved according to the invention in that, during or after process step c) (placing the second plate to form the gap volume and sucking up preparations and possibly test agents into the gap volume), the two plates are either permanently locked together are or are changed irreversibly and visibly, an irreversibly visible change being formed, for example, by broken locking lugs, or also by test liquid penetrating into a gap, which causes a visible change that can no longer be removed.
  • the method can be supplemented by inserting the plate or the two plates into a frame (claim 21), this method step preferably before step (a) (in which case only one plate is first inserted into the frame) and / or is carried out after step c), with the gap volume then being changed in this way after the second plate has been placed on the first plate already inserted in the frame or after the two plates have been inserted in the frame and before step d) can be guided by cycling the panels perpendicular to their planes t to thereby mix the
  • test agent (claim 22). This can be done through the frame (claim 23).
  • step c If the two plates are locked together in accordance with step c), they can no longer be reused, but must be disposed of in closed condition after step d). This is cheap in that z. B. in ⁇ fectious test material is not enclosed by the plates until disposal and operating personnel ⁇ is endangered, furthermore the attempt at recycling is thwarted. An additional marking of used recordings can be omitted here.
  • 1 shows a perspective illustration of a first embodiment of a specimen slide with columnar, round cross-sectional receptacles with a flat receiving surface and a second plate partially shown
  • 2 shows another embodiment of a specimen slide, in which the images have been created by introducing intersecting grooves in a substrate and thereby also form flat image surfaces, with an indicated second plate and an indicated optical scanning device for the individual images;
  • a slide which also has a flat receiving surface, but the second plate has a surface adapted to this slide shape, the plates are either made of solid material (left part view) or of thin-walled material by forming Bumps have developed (right illustration);
  • Fig. 4 is a sectional view taken along lines IV-IV of Fig. 3;
  • FIG. 6 is a sectional view of a slide plate known per se and having a plurality of receptacles, the receptacles having a relatively large volume, the outer bottom surface of this known slide being placed as a second plate;
  • FIG. 8 shows a section through a specimen slide according to FIG. 7, in which, however, the second plate is designed such that it largely fills the specimen slide cavities and thereby forms the thin gap space according to the invention, again possibly with simultaneous production of a lens;
  • Fig. 9, 10, 11, 12 and 13 schematically different ways to move the two plates against each other against spring force
  • FIG. 14 shows an enlarged representation of a receptacle provided here with a flat surface, which has a capillary-like depression or gap for marking the use that has taken place;
  • 15 is a perspective view of two strip-like plates, each provided with five receiving devices, which fit together and are inseparable from one another;
  • FIG. 16 shows an enlarged partial section through the plates according to FIG. 14;
  • Fig. 17 is a perspective view of a frame for receiving z. 14, as an alternative to the introduction of plates with only one receptacle or of plates with z. B. double or triple strips, the frame can either have only a lower part or a hinged to the lower part of the cover, as can be seen here; and - Ig -
  • Fig. 1 8A, B and C in a top view, side view with enlarged detailed representations and in a front view a practical embodiment of a lower strip part (lower strip-shaped plate), which is injection molded from polyamide, for example, and has locking devices on both narrow sides and with ten receiving surfaces for preparations and is equipped with a marker;
  • FIG. 20A, B, C and D a top view and a view from above or from the right according to FIG. 20A and an enlarged view of a corner area of a lower frame part, again z. B. made of polyamide, in which several (here six) strips can be inserted according to Figures 18 and 19.
  • 22A, B are perspective views of a possible hinge connection between the two frame parts, once in the opened state and once in the closed state;
  • a slide 10 for wet or moist preparations, for. B. chemical or biological preparations, such as blood, for which a blood group determination is to be made shown, consisting of a plate 12 made of plastic, with a plurality of columnar projections 16 extending from the surface 14, each of which has a receptacle 18 for the preparation form, which can be dripped onto the flat surface of the receptacle 18 with conventional devices, for example with pipettes, as is also indicated schematically in FIG. 8.
  • a second plate 20 that can be placed on the first plate 12, the surface 22 of which is so designed (namely that it is flat overall) that it matches the surfaces 24 of the receptacles 18, which are also flat, of the first plate 12 each form a (here also flat), gap-extending gap 30 with such a gap width 26 that the volume of the preparation, which is designated 28 in FIG. 8, for example, essentially fills the gap space 30.
  • the (liquid) material is held in the gap, even if the two plates are now handled in some form, for example placed in an evaluation device. Even when the two plates are held at an angle, the material thus remains in the area of the receiving space 18 and does not flow into the area of other receptacles.
  • the embodiment according to FIG. 2 is particularly space-saving because empty spaces lying between the individual receiving surfaces 127 are small.
  • the arrangement is also particularly well suited to enable automatic scanning of different samples, for example in that an optical evaluation device 36 scans the individual recording areas 118 individually in rows or columns, as indicated, for example, by the directional arrows 38.
  • FIG. 9 in a side view, plate 112 and plate 20 can again be seen, with a gap width 26 shown here in a greatly exaggerated manner.
  • This gap width can be defined, for example, by projections 42, which are preferably located near the edge of one or the other also both plates are located. If one now presses the two plates against one another, the distance 26 can be reduced, for example as a result of the flexibility of the plate material, as a result of which the gap volume is also included 11 -
  • the recordings 118 decreases. If you let go again, the distance increases and the gap volume increases. As a result of this movement of the plates perpendicular to and away from the plate plane, see also arrows 44, the preparation liquid (possibly including the test liquid) is spread out in the gap space volume and contracted again, spread out again and contracted again, which causes an extraordinarily strong mixing action.
  • Appropriate stop devices for example also in the form of projections 42, can thereby ensure that the change in gap volume complies with a favorable measure, for example it will be advantageous if the gap space volume can be increased and / or reduced by approximately a factor of 2 compared to the preparation volume.
  • an arrangement such. B. could serve as a hinge (142. Fig. 13) and the other as a hinged closure, and which are designed so that they allow a substantially parallel movement of the two plates to each other.
  • the configuration is such that the projecting lugs 242 can withdraw into corresponding recesses 243, so that the two plates can come directly to each other in their edge regions, in which position a preferred volume size for the gap space volume could be provided.
  • guide devices e.g. pin 443 in FIG. 11
  • connecting devices e.g. connecting devices and the like.
  • ⁇ Conceivable between the two plates, also stackable arrangements, as it is described in more detail in the prior art, so that further explanations are omitted.
  • FIG. 1 and 2 may consist essentially of solid injection molded plastic, but may also be cast and grindable from glass
  • FIG. 3 shows an embodiment in which the receptacles 218 are made from one position existing plastic base plate 212 pressed out bulges are formed. This embodiment saves material and is particularly suitable as a disposable item.
  • the second plate 220 can in turn consist of a flat plate, as in FIG. 1 or FIG. 2, or, as shown in FIG. 3, of a plate which in turn has bulges but is congruent with the bulges of the plate 212, see right side of FIG. 3.
  • Such an embodiment can also be used in reverse, in which the plate 220 forms the receptacle (as a depression) and the plate 212 represents the second plate which forms the gap space 230 when placed on the first plate forms.
  • the construction shown has the advantage that a mutual guidance between the two plates automatically results in that the protruding expressions or bulges of the plate 212 are encompassed by the recessed expressions of the plate 220.
  • the two plates are shown as solid plates, an embodiment which is also possible and of which a sectional view along lines IV-IV is shown in FIG. 4. 4 (or according to the left half of FIG. 3) has a preparation 28 and a test liquid separated from it dripped onto the receptacle 318 according to FIG. 4, with one (or more) further test liquid 48 (see FIG. 8) could be dripped on, or a test preparation already manufactured by the manufacturer, e.g. B. is applied as a gelatin layer.
  • the arrangement according to the invention should (and can also be) used in such a way that two or more than two liquids are used Components are first dripped onto the receptacle 118 separately, then the second plate is placed, the overall arrangement is brought under the observation device and then the mixing process is only triggered by moving the two plates towards one another and the reaction is thus initiated, with the observation then being carried out attention the reaction process is observable.
  • FIG. 5 shows an arrangement in which the gap space 330 is curved in a view similar to FIG. 3, the receiving surface being able to form a concave curvature or a convex curvature depending on the position of the two plates.
  • FIG. 6 An arrangement can be seen in FIG. 6, in which one of the plates, namely the second plate 420, represents an arrangement which is known per se, but which is used here “inappropriately”, namely in such a way that the receiving spaces are not so are used, but the usually unused outer surfaces of the floor form the cover for the receptacle 418 for the preparation by a ZH -
  • the first plate 412 or 512 can be formed, the plate 512 forming a lens-like device in the area of the receptacle 418, by means of material thickening 48.
  • the advantage of this arrangement is that devices that are already commercially available can be used once for regular tests, in which the receiving space 50 is used with its large volume, as previously, or, as shown in FIG. 6, arranged in reverse and used with an additional plate 412 or 512 to create the gap space 418 , with the associated advantages of the substantially lower material consumption, the improved and possibly the later to be carried out mixture and possibly the improved optical monitoring by z.
  • the preparation (eg 28) is introduced into the receiving space 50, which is shown here with a round bottom, then a test agent 46, possibly another test agent 48, where every two or three substances z. B. can be introduced by means of conventional pipettes 52, then the second plate 620 is then placed, which has such a shape in the manner already described that there is a gap 30 in which the preparation 28, optionally with mixing with test liquid 46th , 48 with simultaneous mixing, as shown in Fig. 8, center.
  • Optical devices indicated schematically by the lens 54, which in turn can be formed by a thickening 148 of the plate 620 itself, see left in FIG. 8, then an observation of the preparation 30 by the Material of the plate 620 possible.
  • a part of a plate 12 can be seen in FIG. 14, with a projection 16 which builds on it and forms a receptacle 18.
  • a lateral or ring-shaped capillary-like depression or gap 56 or 156 which is arranged in the vicinity of the edge of the receiving surface 24, emanates from this receptacle and can also have a different shape, but must be arranged in such a way that the receiving surface 18 applied sample or test material has the opportunity to penetrate to a small extent into this gap or this depression 56, 156 as a result of capillary action, in such a way that a residue is retained in this gap even when it dries out or is washed out. If the gap is appropriately fine and the capillary action is correspondingly large, this can easily be achieved.
  • the material 16 is preferably transparent, material that has entered the slit 56, even if it is also transparent and colorless, changes the refraction ratios within the slit 56 such that the presence of this material can be seen by the eye or optical devices. This is particularly true when the material adhering in the gap 56 is colored, such as blood, which causes a red marking in this gap 56 as a result of the red blood cells, so that it can be seen immediately that the projection 16 with its receptacle 18, for example, for Blood tests have already been used. With a larger number of such projections 16, as shown for example in FIG. 1, it can therefore be seen which of these several projections have already been used and should not be used again.
  • FIG. 15 shows a lower plate 712, which can be designed similarly to the plate 212 according to FIG. 3, and an upper plate 714, which can be configured quite analogously, that is to say again how the plate 212 according to FIG. 3 can be shaped.
  • the plate as shown in FIG. 15, has five receptacles 718 lying next to one another, each with a flat surface here, locking and / or guiding devices being carried at both ends of this strip-like structure, which on the one hand move up and down enable the two plates 712, 714 similar to the type shown in FIGS. 9 to 13, but at the same time lock the two plates 712, 714 together in such a way that they cannot subsequently be taken apart and used again.
  • These devices 742, 743 can be configured differently on the left and on the right side of the strip-like plate 712 and 714, for example, twice on one side and only one on the other side, as can be seen in FIG. 15 is, or also be designed differently in some other way, so as to ensure that the two plates 712, 714 can, if necessary, be placed one on top of the other in only one orientation.
  • the handling of the strips according to the invention can be simplified even further if the strip or plate, for example comprising several recordings, has another Has area that can be used for identification, for example to identify the patient belonging to the blood sample.
  • FIG. 15 can be seen in an enlarged partial sectional view in FIG. 16, wherein a special embodiment of the locking and guide devices 742 and 743 described can be seen here: the locking takes place here in that a U-shape designed receiving space of the plate 712 is equipped with projections 68, which lie behind recesses 70, which is formed by an arrow-like projection 743 of the plate 714.
  • This arrow-like projection tapers to a point and can also have an incision 72, so that the penetration of this arrow-like projection 763 into the U-shaped space 742 is made possible.
  • the plates 712 and 714 can still have stiffeners at the edge, for example by flanging 74.
  • FIG. 17 76 In order to be able to make the material of this disposable arrangement according to FIG. 15 even thinner and thereby reduce the use of material without making handling more difficult, a further configuration in the form of a frame arrangement consisting of a base frame is conceivable according to FIG. 17 76 and possibly a cover frame 78, the two frames 76, 78 being able to be connected by a hinge 80 if desired, so that the two frames 76, 78 can be opened and closed in a convenient manner.
  • the base frame 76 either forms the same or, as here, also differently designed receiving spaces 82, 84 and 86 for test plate arrangements of different sizes.
  • a frame 76 according to FIG. 17 without intermediate walls 88 would, for example, be suitable for receiving a test plate 12 according to FIG.
  • the frames 76, 78 are designed in such a way that the optical inspection can be carried out unhindered, for which purpose the frames 76, 78 are either themselves made of transparent material again, or corresponding openings in the frame base 290 or lid base 94 are located.
  • trough-like, elongated receiving spaces 84 can also be provided next to one another, for example to accommodate strip-shaped plates or plate combinations, as shown in FIG. 15. Again, one plate could be accommodated in frame 76, the other plate in frame 78.
  • the bottom 92 could also be designed by means of pin-like projections in such a way that such cups or bowls are nonetheless held in place without intermediate walls 88, while differently designed strip-shaped receptacles, for example having the shape of FIG. 15, also have such projections 76 so that with a single frame structure according to FIG. 76 either large-area recordings, or strip-shaped recordings or individual cup-shaped or cup-shaped recordings are securely held, while the upper frame 78 then has the appropriate recording devices, for example, a single part 620 according to FIG. 8, so that the sample 28 receives the desired flat spread within a capillary space 30.
  • the lens-like device 54 could then also be carried by the frame 78.
  • the strap parts 76, 78 are reusable, even if the receiving devices used therein, for example for blood samples, are disposable items according to FIG. 15.
  • FIGS. 18 to 23 show in various views and representations a possible practical embodiment of a slide which can be inserted in a frame.
  • 18A, 18B and 18C show in a top view, a side view and a view from the right a first (lower) strip-shaped plate 812, pressed from approximately 1 mm thick plastic material (such as polyamide), the strip 812 as a whole here 10 juxtaposed receptacles 818, here of square shape, which, like in the embodiment according to FIGS. 15 and 16, are formed by material pushed upwards.
  • the strip 812 has raised edges 875, 877, 879 which surround the receiving surfaces 818 in a box-like manner.
  • Flag-like projections 881, 883 also emanate from the end face edge regions 877 and 879, which on the one hand facilitate handling, and on the other hand can serve as a marking area for patient data, sample data and also for the dispensing of any test chemicals that may be present. From these edges 877, 879, lugs 885, 887 protrude inwards, with which lugs corresponding lugs 889, 890 of the second (upper) plate or strip shown in FIGS. 19A, 19B and 19C can hook, so that the two strips to lock in their superimposed position with each other. In the locked position, the receiving surfaces 814 of the strip 812 are aligned with the corresponding receiving flat 818 of the strip 814.
  • the strip 818 which also has a box-like stiffening by the edges 891, 893, 895 , with this box shape can be inserted into the box formed by the strip 812.
  • approaches 882, 884 are present, which on the one hand serve as alignment and locking means when inserting this strip into the corresponding frame 878 according to FIG. 21A, see the depressions 982 there, but on the other hand also serve for marking purposes in the manner already described can.
  • the strip 814 serves a resiliently attached nose 896 on the two ends of the strip 814, which lies with its surface 897 behind a corresponding surface 997 of a projecting edge 996 of the frame 878 and thereby after the Bringing the strip into the two recesses 982 of the frame 878 and locking the strip by means of these lugs 296 behind the strip 996 the strip is firmly connected to the frame and can no longer fall out, even if, for example, this upper frame is folded down, as can be seen, for example, in FIG. 22B.
  • the frame consists essentially of solid material, preferably again plastic, which can also be transparent, the construction details being shown in the figures.
  • Strips and frames are each constructed so that they cannot be mixed up, i.e. H. that a lower strip cannot be placed in the upper frame.
  • a depression 1082 is provided in the lower frame, see FIGS. 20A to D, which is so wide that it is able to accommodate the corresponding flag-like extension 883 of the lower strip 812, while the corresponding extension 884 of the upper strip 814 does not fit here and is therefore immediately recognizable that the wrong strip is inserted.
  • This also applies conversely to the lower frame, in which only the lower strip fits, while the upper strip cannot be inserted here.
  • the lower strip 812 also has a locking projection 898 in order to fix the strip in the lower frame accordingly, for which purpose it has a projecting strip 1096 with a lower contact surface 1097, see FIG. 20B.
  • the projection 898 of the lower strip is shaped differently than the speaking projection 896 of the upper strip, the design being selected such that the holding force in the frame for the lower strip is slightly less than the holding force of the upper strip within the upper frame.
  • the lower strip is placed in the lower frame, the strip being jammed in the frame with the aid of the projections 898 to such an extent that it can no longer fall out of its own accord, and then the upper strip 814 in the upper frame is inserted at the corresponding point aligned with the lower strip and clamped by means of the projection 896, the frames 876 and 878 according to FIG. 22 a being able to lie flat on the work surface in this insertion position.
  • the samples are then applied to the receptacles 818 of, for example, the lower strip, a test liquid is applied to the corresponding receptacles on the upper strip, if this does not already contain a test substance applied in the factory, and the upper frame is then placed on the 22B, whereby the two strips with their receptacles 818 come together and interlock and lock with one another with their projections 887 and 890.
  • the sample substance and test substance react with one another and give, for example, a precipitate that is visible through the transparent strip material surface.
  • the upper frame is removed from the lower frame again, for example by opening it again, whereupon the interlocked strips come loose from one of the frames, for example here from the lower frame, so that the interlocked strips in the upper one Frame remain and either with this to z.
  • Control purposes are kept or removed from them and disposed of.
  • FIG. 23A shows a frame consisting of the frame parts 876 and 878 with a joint 880, the frame part 876 at its end carrying the joint 880 merging into an attachment 802 which is U-shaped in cross section and which is open with its cross section Is directed towards the outer surface 803 of the frame part 876 and, with its closed end 804, projects beyond the inner surface 805 receiving the test strips to form a pivot bearing for the other frame part 878.
  • a bearing pin or pin 806 which can be slidably and rotatably received and which is fastened at both ends to a retaining tab 807 or merges into one which in turn laterally adjoins the (upper according to FIG 23B) frame part 878 is fastened, for example screwed by means of screws 808.
  • the bearing pin 806 has a radial depression 809 near each of its two ends (there may be further recesses in between), which is oriented such that it points towards the open end of the U when the frame is closed and towards a pin or a screw 811 is aligned, which in the position shown in FIG.
  • the bearing pin 806 is held in the position shown by a spring element 813, which is present, for example, in the form of a spiral spring placed around the pin 811, which is supported on the one hand on a locking strip 815 for the U-profile and on the other on the circumferential surface of the bearing pin 806 or on an intermediate piece slidably placed on this circumferential surface, not shown.
  • a spring element 813 which is present, for example, in the form of a spiral spring placed around the pin 811, which is supported on the one hand on a locking strip 815 for the U-profile and on the other on the circumferential surface of the bearing pin 806 or on an intermediate piece slidably placed on this circumferential surface, not shown.
  • This movement is used for mixing, for example, as already described.
  • the construction described allows a stable guidance and alignment of the two frame parts 876, 878 to one another, so that it is ensured that, even in the case of very small receiving areas, the preparation liquid (on e.g. the lower frame part) and the test liquid (on z. B. the upper frame part) is ensured.
  • the construction also ensures that a uniform parallel guidance is achieved when the two frame parts move relative to one another, and that an identical stroke is achieved for all sample strips and thus also for all individual samples lying opposite one another, so that the mixing processes are the same for all samples and z. B. meet a certain standard, so that it is ensured that different results do not result from different mixing intensities, which do not come about due to differences in preparation.
  • the construction described is also designed in such a way that it does not lead to canting when folded, since the compression of the two frame parts in the direction of arrows 823 is only possible when the two frame parts have reached the closed position, as shown in FIG. 23B.
  • misalignment of bore 809 with pin 811 prevents shaft 806 from escaping the U-shaped end of part 802.
  • the arrangement of a frame in which a large number of individual sample strips, each with a larger number of individual samples in turn, can be accommodated also enables automation which is expedient in many cases.
  • the frame could be placed in a device which accomplishes the mixing process by forcing the lifting movement of the two frame parts toward one another, for example by magnetic devices or by pneumatic drive, for example at a frequency of two lifting movements per second, so that at a stroke rate of about 50 to 60 for a thorough mix, the total treatment time is about 1 / £. Minute would be.
  • a mechanical drive would also enable an even more uniform application of pressure, although an arrangement of compression spring devices 821 in the area of the stops 819, for example, could also remedy this.
  • Such spring devices 821 could, for example, be attached to all four corners of the frame. If their compressive force does not significantly exceed that of the spring 813, so that the influence of the spring 813 is not negligible, such springs could only be arranged at the end facing away from the joint, in particular this applies if their compressive force would correspond approximately to that of the springs 813, so that ultimately a uniform distribution of the back pressure exerted by the springs at the four corners of the frame parts and then the overall springs would result.
  • This uniform pressure at all four corners of the frame facilitates a uniform movement of the frame parts 876, 878 towards one another when this is carried out by hand, while in the case of mechanical operation, corresponding devices can be provided in the drive device which automatically does this.
  • appropriate locking devices for example, suction cups or clamping jaws
  • the drive jaws of a vibrator drive and the externally accessible surfaces of the two frame parts 876, 878 can be achieved that they are forcibly moved with a certain stroke and a certain speed, regardless of the presence of the springs 813, 821.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un porte-objets (10) pour préparations à teneur en eau, par exemple des préparations chimiques ou biologiques, telles que des préparations de sang, comprenant une première plaque (12) en une matière transparente de préférence, notamment en matière plastique transparente, présentant plusieurs logements (18) pour les préparations portés par la surface (14), cependant qu'il est prévu une deuxième plaque (20) applicable sur la première plaque (12) et dont la surface (24) est configurée de telle façon qu'elle forme, avec la surface (22) des logements (18) de la première plaque (12), respectivement, une fente plane ou légèrement incurvée (30) s'étendant à la surface et ayant une largeur (26) telle que le volume de la préparation remplisse sensiblement le volume de ladite fente. Pour la caractérisation de l'utilisation en cours, les plaques présentent un dispositif de verrouillage, ou bien l'utilisation de la plaque est signalée par des marquages se formant en cours d'essai. On utilise à cet effet soit des dispositifs de verrouillage, soit des découpes capillaires (56, 156) s'étendant à partir de la surface des logements.
EP90916289A 1990-05-23 1990-11-12 Porte-objets pour preparations a teneur en eau, par exemple, de sang Expired - Lifetime EP0530186B1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE9005871 1990-05-23
DE9005871U 1990-05-23
PCT/EP1990/001892 WO1991017832A1 (fr) 1990-05-23 1990-11-12 Porte-objets pour preparations a teneur en eau, par exemple, de sang

Publications (2)

Publication Number Publication Date
EP0530186A1 true EP0530186A1 (fr) 1993-03-10
EP0530186B1 EP0530186B1 (fr) 1995-08-30

Family

ID=6854067

Family Applications (1)

Application Number Title Priority Date Filing Date
EP90916289A Expired - Lifetime EP0530186B1 (fr) 1990-05-23 1990-11-12 Porte-objets pour preparations a teneur en eau, par exemple, de sang

Country Status (4)

Country Link
EP (1) EP0530186B1 (fr)
AU (1) AU7042391A (fr)
DE (1) DE59009600D1 (fr)
WO (1) WO1991017832A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE9217130U1 (de) * 1992-12-08 1993-04-08 Astra Chemicals GmbH, 2000 Wedel Vorrichtung zur Durchführung kombinierter Ureasetests von Antrum/Korpus-Biopsien zur Diagnose von Gastrointestinalerkrankungen
DE4405375C2 (de) * 1994-02-19 1996-07-25 Fritz Nerbe Nachfolger Juergen Mikrotiterplatte
US5795748A (en) * 1996-09-26 1998-08-18 Becton Dickinson And Company DNA microwell device and method
WO1999056878A1 (fr) * 1998-04-30 1999-11-11 Graffinity Pharmaceutical Design Gmbh Dispositif pour transporter des liquides le long de voies de guidage predeterminees
DE19827754C1 (de) 1998-06-23 2000-02-10 Graffinity Pharm Design Gmbh Einrichtung für eine nahezu gleichzeitige Synthese einer Vielzahl von Proben
DE19907011A1 (de) * 1999-02-18 2000-08-31 Deutsches Krebsforsch Fluoreszenzkorrelationsspektroskopievorrichtung und -verfahren, insbesondere zur Mehrfarbenfluoreszenzkorrelationsspektroskopie

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0111762B1 (fr) * 1980-06-20 1987-11-19 Unilever Plc Procédés et appareil pour l'exécution d'essais de liaisons spécifiques
GB2147698B (en) * 1981-11-17 1986-05-08 Unilever Plc Test apparatus for immunoassay
DE3505892A1 (de) * 1985-02-20 1986-08-21 GVB SANIMED Hygiene- und Medizintechnik GmbH, 3070 Nienburg Verschliessbarer behaelter zur aufnahme von laborproben, sondermuell oder dergleichen
US4721679A (en) * 1986-10-23 1988-01-26 Yiu Felix H F Tissue typing tray

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9117832A1 *

Also Published As

Publication number Publication date
DE59009600D1 (de) 1995-10-05
AU7042391A (en) 1991-12-10
EP0530186B1 (fr) 1995-08-30
WO1991017832A1 (fr) 1991-11-28

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