EP0374197A4 - Enzymatic production of maltohexaose-rich compositions - Google Patents
Enzymatic production of maltohexaose-rich compositionsInfo
- Publication number
- EP0374197A4 EP0374197A4 EP19890901956 EP89901956A EP0374197A4 EP 0374197 A4 EP0374197 A4 EP 0374197A4 EP 19890901956 EP19890901956 EP 19890901956 EP 89901956 A EP89901956 A EP 89901956A EP 0374197 A4 EP0374197 A4 EP 0374197A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- starch
- maltohexaose
- amylase
- enzyme
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Malto-oligosaccharide compositions which contain up to about 40 % maltohexaose by weight are produced from starchy substrates and maltodextrins by a simple, one-step hydrolysis with certain thermostable alpha-amylase from Bacillus stearothermophilus. This process is particularly useful in the production of novel compositions with properties that will lead to new applications in both food and nonfood industries.
Description
ENZYMATIC PRODUCTION OF
MALTOHEXAOSE-RICH COMPOSITIONS
Background of the Invention Field of the Invention
Malto-oligosaccharides, also referred to as maltodextrins and dextrose syrup solids, are produced from starch by hydrolysis with α-amylases. These carbohydrates are used in adhesives and in food applications such as syrups, flavor encapsulation, texture control, binding agents, carriers for low-calorie sweeteners, and gels in reduced-calorie foods. This invention relates to the production of novel malto-oligosaccharide compositions that contain a large proportion of maltohexaose. Description of the Prior Art
Thermally stable α-amylases have enabled a rapid advance in the commercial production of malto-oligosaccharides by enzymatic hydrolysis of starch. However, the mode of the amylase action on starches is only partly understood because the fine structure of starches is still obscure. Furthermore, amylases from different soυrces behave differently, and their action patterns are dependent on reaction conditions. Certain amylases are now known to yield distinctive patterns of malto -oligosaccharide products that are at variance with the distribution of products that would be predicted on the basis of randan cleavage of starch molecules. Robyt et al. [Arch. Biochem. Biophys. 100: 451-467 (1963)] teach that α-amylase from Bacillus subtilis selectively forms maltotriose and maltohexaose. Nakakuki et al. [Carbohydr. Res. 128 : 297-310 (1984)] report that the α-amylase from B. licheniformis degrades short-chain amylose at 1% concentration and
40* C to give mainly maltopentaose and maltotriose with slightly smaller quantities of maltose. In contrast, Inglett [J. Food Biochem. 11 : 249-258 (1987) ] shows that this same enzyme, acting on higher substrate concentτations (20-30% starch) and at a higher temperature (95º C), yields increased quantities of maltose,
essentially equivalent to or slightly higher than the other two oligomers. Slomin'ska et al. [Starch/Starke 38(6): 205-210 (1986) ] show tht a 72-hr saccharification of liquified starch with a thermostable maltogenic amylase from B . stearothermophilus virtually eliminates the maltohexaose (G6) constituent. Outrup et al. [Starch/Starke 36(12): 405-411 (1984) shows that treatment of amylopectin with a B . stearothermophilus amylase produces only traces of maltohexaose (G6). Summary of the Invention
I have now surprisingly found that certain thermostable α-amylases have a unique and unexpected .action on starches and maltodextrins to produce large quantities of maltohexaose [degree of polymerization (DP) 6], with comparatively minor amounts of oligomeric constituents of DP greater than 6. These amylases have utility in a process for converting starch into products with potentially unique and expanded markets.
In accordance with this discσvery, it is an object of the invention to provide a simple, one-step method for converting starch into novel malto-oligosaccharide conpositions that contain a large proportion of maltohexaose.
It is also an object of the invention to provide novel maltodextrins and dextrose syrup solids containing maltohexaose as the dominant oligomer and with properties leading to potential new uses.
Other objects and advantages of the invention will become readily apparent from the ensuing description. Detailed Description of the Invention Suitable starting materials contemplated for use in the invention include unmodified natural granular starches such, as regular cereal, potato, and tapioca starch, as well as waxy starches and high-amylose starches. These materials are prepared for enzyme treatment by gelatinization. For purposes of this invention, gelatinizatian is accomplished preferably by passage of an aqueous slurry of the starch through a steam-injection cooker at a temperature of about 120°-165º C to ensure thorough dispersion of the starch.
Other methods of gelatinization are well-known in the art. Alternatively, pregelatinized starch and maltodextrins would serve as useful starting materials. The concentration of substrate should be in the range of about 5-45% by weight.
A suitable calcium salt is added to the aqueous dispersion of substrate in an amount sufficient to stabilize the subsequently added α-amylase (preferably about 50 ppm of calcium). The pH of the resulting starchy dispersion is adjusted to about 6.0 with sodium hydroxide or other alkali, and the dispersion is treated at a temperature in the range of 70°-100º C, preferably about 95º C, with a thermostable α-amylase.
The thermostable α-amylases useful herein are those referred to as 1,4-alpha-D-glucan glucanohydrolases and having the essential enzymatic characteristics of those produced by the B . stearothermophilus strains ATCC Nos. 31,195; 31,196; 31,197; 31,198; 31,199; and 31,783. These strains are described in U.S. Patent No. 4,284,722, which is herein incorporated by reference. Other sources of this enzyme include organisms such as B . subtil is which have been genetically modified to express the thermostable α-amylase of B . stearothermophilus as described in U.S. Patent No. 4,493,893, herein incorporated by reference. These enzymes are available commercially under the name "Enzeco Thermolase" (Enzyme Development, Div., Biddle Sawyer Corp., New York, N.Y.).
The level of enzyme suitable for use in this process is generally in the range of about 3-25 units per g of starch or dextrin, where 1 unit of bacterial α-amylase activity is the amountof enzyme required to hydrolyze 10 mg of starch per minute under specified conditions [Enzyme Development, Div. , Biddle Sawyer Corp. , New York, N.Y., Technical Bulletin No. 20 (Revised 7/86)]. Similarly, the duration of treatment depends on the product desired and will generally range from about 10-60 min; with 30-50 min being preferred for enzyme concentrations in the range of 10-20 units/g substrate; and 10-30 min being preferred for enzyme concentrations in the range of 20-25 units/g substrate.
After the desired conversion time, it is preferable to decolorize the resulting mixture with activated carbon and add a filter aid to facilitate subsequent recovery of the hydrolyzate. The pH is then adjusted to 3.5-4.0, such as with 0.2 N sulfuric acid, and the product is heated at about 95º C for 10 min to inactivate remaining enzyme. The pH is then adjusted to about 6.5, .such as with 1 N sodium hydroxide, and the product is separated by filtration and then dried by any of a variety of techniques as within the skill of the person in the art.
The products of this invention differ from commercially available maltodextrins and dextrose syrup solids in that the latter products contain a fairly uniform distribution of oligosaccharides with no preponderance of any particular oligomer. It is therefore envisioned that the maltohexaose-rich products of this invention will have unique properties that will lead to new food applications in fields such as flavor encapsulation, texture control, and binding agents, as well as new industrial applications. These products might also serve as starting materials in new procedures for preparing carbohydrate compositions such as cyclodextrins.
The following examples are presented only to further illustrate the invention and are not intended to limit the scope of the invention which is defined by the claims.
All percentages herein disclosed are by weight unless otherwise specified.
Example 1
Standard Process Conditions. Two hundred g (dry basis) of high-amylose corn starch ("Amylomaize VII," American Maize-Products Co., Hammond, IN) was slurried in 800 ml of water containing 50 ppm of calcium (0.185 g/1 CaCl2.2H20) and passed through a steam-injection cooker at 138º-143º C (30-40 psi of steam pressure). The gelatinized starch paste was collected in a Dewar flask, and the pH was adjusted to 6.0 with 1.0 N sodium hydroxide. Thermostable α-amylase ("Enzeco Thermolase," supra) was added to the starch paste at 95º C in an amount sufficient to provide 16.5 units (supra) per g of starch. Samples of converted starch were removed 20, 40, and 60 min after
addition of the enzyme. To each sample was added, with stirring, activated carbon ("Darco G-60," E M Science, Div., E M Industries, Inc., Cherry Hill, NJ) sufficient to provide a concentration of 0.1%, and filter aid ("Hyflo Filter Cel," Manville, Fitration & Minerals Div., Denver, CO) sufficient to provide a concentration of 2%. The pH was adjusted to 3.5-4.0 with 0.2 N sulfurlc acid, and the products were heated at 95º for 10 min to inactivate remaining enzyme. The pH was then raised to 6.5 with 1 N sodium hydroxide, and the mixtures were filtered hot through a bed of filter aid (supra) on filter paper ("Whatman No. 1," Whatman Chemical Separation Inc., Clifton, NJ) on a Buchner funnel under vacuum. The filtrates were spray-dried (Pulvis Mini Spray Dryer, Model GA-31, Yamato, Northbrook, IL) , and the carbohydrate ccmposition of the products was determined by high-pressure liquid chromatography (Inglett, supra). The analytical results in the Table, below, show that highest yields of maltohexaose were obtained with conversion times of 40 and 60 min.
Examples 2-3 Effect of Enzyme Leval. Compositions were prepared as described in Example 1 except that the α-amylase level was 11.0 units per g of starch in Example 2 and 22.0 units per g in Example 3.
Examples 4-5 Effect of pH. Compositions were prepared as described in Exanple 1 except that the pH during enzyme conversion of the starch was 7.0 in Example 4 and 5.0 in Exanple 5. The results in the Table show that pH 6.0 (Example 1) is the preferred pH, but that slight variations above or below this value would not significantly affect the yield of maltohexaose.
Example 6 Conversion of Potato Amylose. (Compositions were prepared asdescribed in Exanple 1 except that the starchy substrate was potato amylose (Avebe America, Inc., Hopelawn, NJ) instead of high-amylose corn starch. The results in the Table show that yields of maltohexaose from potato amylose were slightly less than those from high-amylose corn starch (Example 1).
It is understood that the foregoing detailed description is given merely by way of illustration and that modification and variations may be made therein without departing from the spirit and scope of the invention.
Table
Enzyma concentrat ion Conversion Amount of constituenta, wt. %
(units/g time
Ex ample substrate) pH (min) DP >9 DP-9 DP-8 DP-7 DP-6 DP-5 DP-4 DP-3 DP-2 DP-1
1A 16.5 6 20 29. 7 0.2 0.8 12.3 22 .0 7.8 6.2 14.0 6.9 0
1B 16.5 6 40 10.9 0 0.2 0 38 .7 12.6 8.9 17.5 10.6 0. 6
1C 16.5 6 60 8. 2 0 0.1 0 37 .1 14.1 9.1 18.1 12.3 1 .0
2A 11.0 6 20 18. 8 0.1 0.7 10.7 25 .8 10.8 8.2 16.5 8.1 0 .3
2B 11.0 6 40 8. 2 0 0 0 34 .8 16.1 9.3 18.7 12.1 0 .9
2C 11.0 6 60 5. 4 0 0 0.1 31 .3 19.1 9.3 19.2 14.0 1 .5
3A 22.0 6 20 6. 2 0 0 0 34 .9 16.4 9.2 18.8 13.3 1 .2
3B 22.0 6 40 3. 6 0 0.1 0.1 28 .1 20.9 9.2 19.0 16.1 2 .9
3C 22.0 6 60 2. 6 0 0.1 0.1 23. 2 21.5 9.2 19.1 18.5 5 .7
4A 16.5 7 20 14. 3 0 0.6 11.8 27 .4 11.0 8.8 16.2 9.3 0.4
4B 16.5 7 40 6. 1 0 0 0 35 .7 15.4 9.6 18.3 13.5 1 .2
4C 16.5 7 60 4. 5 0 0 0 30 .6 18.9 9.9 18.7 15.3 2 .0
5A 16.5 5 20 10 .2 0 0 0.2 39 .7 12.4 8.5 17.8 10.7 0. 6
5B 16.5 5 40 6 .5 0 0 0 35. 6 15.9 9.0 18.7 13.0 1 .2
5C 16.5 5 60 6 .1 0 0 0 34. 8 16.5 9.1 19.0 13.5 1. 1
6A 16.5 6 20 1 .6 0 0 10.1 30. 2 15.2 9.5 20.3 12.2 0. 9
6B 16.5 6 40 1 .0 0 0 0 33 . 1 18.8 9.6 20.4 14.8 2. 3
6C 16.5 6 60 0 .9 0 0 0 30 .7 20.1 9.6 20.4 15.6 2. 8 a DP = degree of polymerization of dextrose, where DP-2 is disaocharide, DP-3 is trisaocharide, etc.
Claims
I claim:
1. A method for producing a maltohexaose-rich composition from a substrate selected from the group of gelatinized starches and maltodextrins comprising treating an aqueous dispersion or solution of said substrate with a thermostable α-amylase having the essential enzymatic characteristics of the thermostable α-amylases produced by B . stearothermophilus strains ATCC No. 31,195; 31,196; 31,197; 31,198; 31,199; and 31,783 for a period of 10-60 min and recovering said maltohexaose-rich composition.
2. The method as described in Claim 1 wherein said starch is selected from the group consisting of regular cereal, potato, and tapioca starches, as well as waxy and high-amylose starches.
3. The method as described in Claim 1 wherein said substrate is a maltodextrin.
4. The method as described in Claim 1 wherein the amount of said α-amylase is sufficient to provide about 3-25 units of the enzyme per g of starch.
5. The method as described in Claim 1 wherein said treatment of starch with enzyme is performed at a teπperature in the range of about 70º-100º C.
6. The method as described in Claim 1 wherein the amylase is present in the amount of 10-20 units/g substrate and the period of enzyme treatment is in the range of about 30-50 min.
7. A composition produced by the method of Claim 1.
8. A composition produced by the method of Claim 2.
9. A composition produced by the method of Claim 3.
10. A composition produced by the method of Claim 4.
11. A composition produced by the method of Claim 5.
12. A composition produced by the method of Claim 6.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US18909388A | 1988-05-02 | 1988-05-02 | |
US189093 | 1988-05-02 |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0374197A1 EP0374197A1 (en) | 1990-06-27 |
EP0374197A4 true EP0374197A4 (en) | 1991-10-02 |
Family
ID=22695905
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19890901956 Withdrawn EP0374197A4 (en) | 1988-05-02 | 1989-01-26 | Enzymatic production of maltohexaose-rich compositions |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP0374197A4 (en) |
AU (1) | AU3033289A (en) |
WO (1) | WO1989010970A1 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3533239B2 (en) * | 1994-03-01 | 2004-05-31 | 株式会社林原生物化学研究所 | Maltohexaose / maltoheptaose-forming amylase, method for producing the same and use thereof |
GB9407104D0 (en) * | 1994-04-11 | 1994-06-01 | Dalgety Plc | Process for the preparation of food ingredients |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61205494A (en) * | 1985-03-11 | 1986-09-11 | Sanmatsu Kogyo Kk | Production of branched dextrin and straight-chain oligosaccharide |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4298400A (en) * | 1971-01-18 | 1981-11-03 | Grain Processing Corporation | Low D.E. starch conversion products |
US4284722A (en) * | 1978-08-16 | 1981-08-18 | Cpc International Inc. | Heat and acid-stable alpha-amylase enzymes and processes for producing the same |
US4241183A (en) * | 1979-04-30 | 1980-12-23 | Grain Processing Corporation | Starch liquefaction process |
US4493893A (en) * | 1981-01-15 | 1985-01-15 | Cpc International Inc. | Process for cloning the gene coding for a thermostable alpha-amylase into Escherichia coli and Bacillus subtilis |
US4717662A (en) * | 1985-01-31 | 1988-01-05 | Miles Laboratories, Inc. | Thermal stabilization of alpha-amylase |
DE3781732T2 (en) * | 1986-07-09 | 1993-03-25 | Novo Industri As | MIXTURES OF ALPHA AMYLASE FOR LIQUIDIZING STARCH. |
-
1989
- 1989-01-26 EP EP19890901956 patent/EP0374197A4/en not_active Withdrawn
- 1989-01-26 WO PCT/US1989/000325 patent/WO1989010970A1/en not_active Application Discontinuation
- 1989-01-26 AU AU30332/89A patent/AU3033289A/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS61205494A (en) * | 1985-03-11 | 1986-09-11 | Sanmatsu Kogyo Kk | Production of branched dextrin and straight-chain oligosaccharide |
Non-Patent Citations (2)
Title |
---|
CHEMICAL ABSTRACTS, Vol. 106, No. 15, April 1987, Columbus, Ohio, USA, YOSHIDA, TSUKASA et al.: "Branched dextrins and straight-chain oligosaccharides", page 506; column 1; Ref. No. 106:118179A, Abstract; & JP,A,61 205 494 (SANKO KOGYO CO., LTD.), 11 Sep. 1986. * |
See also references of WO8910970A1 * |
Also Published As
Publication number | Publication date |
---|---|
EP0374197A1 (en) | 1990-06-27 |
AU3033289A (en) | 1989-11-29 |
WO1989010970A1 (en) | 1989-11-16 |
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