EA202090090A1 - Включение неприродных нуклеотидов и способы с ними - Google Patents

Включение неприродных нуклеотидов и способы с ними

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Publication number
EA202090090A1
EA202090090A1 EA202090090A EA202090090A EA202090090A1 EA 202090090 A1 EA202090090 A1 EA 202090090A1 EA 202090090 A EA202090090 A EA 202090090A EA 202090090 A EA202090090 A EA 202090090A EA 202090090 A1 EA202090090 A1 EA 202090090A1
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EA
Eurasian Patent Office
Prior art keywords
methods
inclusion
natural nucleotides
kits
proteins
Prior art date
Application number
EA202090090A
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English (en)
Inventor
Джерод Птацин
Каролина Каффаро
Ханс Аэрни
Йорке Чжан
Эмиль К. Фишер
Аарон У. Фельдман
Вивиан Т. Диен
Флойд Е. Ромесберг
Original Assignee
Синторкс, Инк.
Дзе Скриппс Рисёч Инститьют
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Синторкс, Инк., Дзе Скриппс Рисёч Инститьют filed Critical Синторкс, Инк.
Publication of EA202090090A1 publication Critical patent/EA202090090A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y601/00—Ligases forming carbon-oxygen bonds (6.1)
    • C12Y601/01—Ligases forming aminoacyl-tRNA and related compounds (6.1.1)
    • C12Y601/01026—Pyrrolysine-tRNAPyl ligase (6.1.1.26)

Abstract

В изобретении раскрыты способы, композиции и наборы для синтеза белков, которые включают в себя неприродные аминокислоты, которые используют мутантную tRNA.
EA202090090A 2017-07-11 2018-07-10 Включение неприродных нуклеотидов и способы с ними EA202090090A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762531325P 2017-07-11 2017-07-11
PCT/US2018/041509 WO2019014267A1 (en) 2017-07-11 2018-07-10 INCORPORATION OF NON-NATURAL NUCLEOTIDES AND ASSOCIATED METHODS

Publications (1)

Publication Number Publication Date
EA202090090A1 true EA202090090A1 (ru) 2020-06-09

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EA202090090A EA202090090A1 (ru) 2017-07-11 2018-07-10 Включение неприродных нуклеотидов и способы с ними

Country Status (15)

Country Link
US (4) US20200131555A1 (ru)
EP (1) EP3652316A4 (ru)
JP (2) JP7325341B2 (ru)
KR (2) KR20240038157A (ru)
CN (1) CN111051512A (ru)
AR (1) AR112756A1 (ru)
AU (1) AU2018300069A1 (ru)
CA (1) CA3069321A1 (ru)
EA (1) EA202090090A1 (ru)
IL (1) IL271903A (ru)
MA (1) MA49578A (ru)
NZ (1) NZ761479A (ru)
SG (1) SG11202000167SA (ru)
TW (1) TWI821192B (ru)
WO (1) WO2019014267A1 (ru)

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