EA201691208A1 - Фумаратредуктазы - Google Patents

Фумаратредуктазы

Info

Publication number
EA201691208A1
EA201691208A1 EA201691208A EA201691208A EA201691208A1 EA 201691208 A1 EA201691208 A1 EA 201691208A1 EA 201691208 A EA201691208 A EA 201691208A EA 201691208 A EA201691208 A EA 201691208A EA 201691208 A1 EA201691208 A1 EA 201691208A1
Authority
EA
Eurasian Patent Office
Prior art keywords
dependent
fumarate reductase
reductase activity
fumarateductases
activity
Prior art date
Application number
EA201691208A
Other languages
English (en)
Other versions
EA037088B1 (ru
Inventor
Рене Марсель Йонг Де
Чжэн Чжао
Бен Дюлк ден
Ремко Тшиббе Винтер
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201691208A1 publication Critical patent/EA201691208A1/ru
Publication of EA037088B1 publication Critical patent/EA037088B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
    • C12Y103/01—Oxidoreductases acting on the CH-CH group of donors (1.3) with NAD+ or NADP+ as acceptor (1.3.1)
    • C12Y103/01006—Fumarate reductase (NADH) (1.3.1.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Настоящее изобретение относится к вариантному полипептиду, имеющему активность фумаратредуктазы, которая имеет модифицированную NADP(H)-зависимую и/или NAD(H)-зависимую активность по сравнению с эталонным полипептидом, имеющим активность фумаратредуктазы. Такой вариант может быть сверхэкспрессирован в клетке-хозяине для улучшения выработки дикарбоновой кислоты.
EA201691208A 2013-12-12 2014-12-12 Фумаратредуктазы EA037088B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13196950 2013-12-12
PCT/EP2014/077638 WO2015086839A1 (en) 2013-12-12 2014-12-12 Fumarate reductases

Publications (2)

Publication Number Publication Date
EA201691208A1 true EA201691208A1 (ru) 2016-12-30
EA037088B1 EA037088B1 (ru) 2021-02-04

Family

ID=49726663

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201691208A EA037088B1 (ru) 2013-12-12 2014-12-12 Фумаратредуктазы

Country Status (8)

Country Link
US (1) US9944905B2 (ru)
EP (1) EP3080251B1 (ru)
CN (2) CN105814198A (ru)
BR (1) BR112016013526A2 (ru)
CA (1) CA2931591C (ru)
EA (1) EA037088B1 (ru)
ES (1) ES2790573T3 (ru)
WO (1) WO2015086839A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3485004B1 (en) * 2016-07-13 2021-09-22 DSM IP Assets B.V. Malate dehyrogenases
US20210207076A1 (en) * 2018-05-25 2021-07-08 Danisco Us Inc. Overexpression of fumarate reductase results in an increased fermentation rate in yeast
CN114806913B (zh) * 2022-04-15 2023-11-28 盛虹控股集团有限公司 具有线粒体定位还原tca途径的高产琥珀酸酵母工程菌株及其构建方法和应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2522622T3 (es) * 2007-11-20 2014-11-17 Dsm Ip Assets B.V. Producción de ácido succínico en una célula eucariota
EP2297297B1 (en) * 2008-07-08 2017-02-08 DSM IP Assets B.V. Succinic acid production by fermentation at low ph
WO2010111344A2 (en) * 2009-03-24 2010-09-30 Microbia, Inc. Methods and microorganisms for production of c4-dicarboxylic acids
WO2010118932A1 (en) * 2009-04-15 2010-10-21 Dsm Ip Assets B.V. Dicarboxylic acid production process

Also Published As

Publication number Publication date
WO2015086839A1 (en) 2015-06-18
ES2790573T3 (es) 2020-10-28
CN112094826A (zh) 2020-12-18
CN105814198A (zh) 2016-07-27
WO2015086839A9 (en) 2016-09-09
US9944905B2 (en) 2018-04-17
BR112016013526A2 (pt) 2017-10-03
CA2931591C (en) 2023-09-19
EA037088B1 (ru) 2021-02-04
EP3080251A1 (en) 2016-10-19
CN112094826B (zh) 2023-12-19
US20160319245A1 (en) 2016-11-03
CA2931591A1 (en) 2015-06-18
EP3080251B1 (en) 2020-03-04

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