EA201370070A1 - СПОСОБ ДЕТЕКЦИИ, ДИФФЕРЕНЦИАЦИИ И КВАНТИФИКАЦИИ ПОПУЛЯЦИЙ Т-КЛЕТОК С ИСПОЛЬЗОВАНИЕМ КОЛИЧЕСТВЕННОЙ ПЦР С ОБРАТНОЙ ТРАНСКРИПЦИЕЙ В РЕАЛЬНОМ ВРЕМЕНИ (ОТ-колПЦР РВ) - Google Patents
СПОСОБ ДЕТЕКЦИИ, ДИФФЕРЕНЦИАЦИИ И КВАНТИФИКАЦИИ ПОПУЛЯЦИЙ Т-КЛЕТОК С ИСПОЛЬЗОВАНИЕМ КОЛИЧЕСТВЕННОЙ ПЦР С ОБРАТНОЙ ТРАНСКРИПЦИЕЙ В РЕАЛЬНОМ ВРЕМЕНИ (ОТ-колПЦР РВ)Info
- Publication number
- EA201370070A1 EA201370070A1 EA201370070A EA201370070A EA201370070A1 EA 201370070 A1 EA201370070 A1 EA 201370070A1 EA 201370070 A EA201370070 A EA 201370070A EA 201370070 A EA201370070 A EA 201370070A EA 201370070 A1 EA201370070 A1 EA 201370070A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- aliquot
- pcr
- cell populations
- detection
- antigen
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Hematology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Заявленное изобретение относится к способу детекции, дифференциации и квантификации популяций Т-клеток, включающему следующие этапы: а) обеспечение наличия контакта первой аликвоты биологической жидкости пациента как минимум с одним антигеном, причем биологическая жидкость содержит антигенпрезентирующие клетки (АПК) и Т-клетки; б) инкубирование первой аликвоты как минимум с одним антигеном в течение определенного периода времени; в) детекция и дифференциация популяций Т-клеток путем детекции как минимум первого маркера АПК, индуцированных Т-клетками специфической популяции Т-клеток в первой аликвоте и во второй аликвоте биологической жидкости пациента, которая не была инкубирована как минимум одним антигеном, с использованием количественной ПЦР с обратной транскрипцией в реальном времени (ОТ-колПЦР РВ); и г) детекция и квантификация популяций Т-клеток путем определения соотношения распознанного маркера АПК в первой аликвоте ко второй аликвоте, а также комплект для практического осуществления заявленного способа.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102010037622A DE102010037622B4 (de) | 2010-09-17 | 2010-09-17 | Verfahren zum Nachweis, Differenzieren und Quantifizieren von T-Zellpopulationen mittels der Reverse Transkription quantitativen Real-Time PCR (RT-qPCR) Technologie |
PCT/DE2011/075225 WO2012037937A2 (de) | 2010-09-17 | 2011-09-16 | Verfahren zum nachweis, differenzieren und quantifizieren von t-zellpopulationen mittels der reverse transkription quantitativen real-time pcr (rt-qpcr) technologie |
Publications (1)
Publication Number | Publication Date |
---|---|
EA201370070A1 true EA201370070A1 (ru) | 2013-07-30 |
Family
ID=45491185
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201370070A EA201370070A1 (ru) | 2010-09-17 | 2011-09-16 | СПОСОБ ДЕТЕКЦИИ, ДИФФЕРЕНЦИАЦИИ И КВАНТИФИКАЦИИ ПОПУЛЯЦИЙ Т-КЛЕТОК С ИСПОЛЬЗОВАНИЕМ КОЛИЧЕСТВЕННОЙ ПЦР С ОБРАТНОЙ ТРАНСКРИПЦИЕЙ В РЕАЛЬНОМ ВРЕМЕНИ (ОТ-колПЦР РВ) |
Country Status (10)
Country | Link |
---|---|
US (1) | US9733246B2 (ru) |
EP (1) | EP2616816B1 (ru) |
JP (2) | JP2013539652A (ru) |
CN (1) | CN103109191B (ru) |
BR (1) | BR112013006240A2 (ru) |
DE (1) | DE102010037622B4 (ru) |
EA (1) | EA201370070A1 (ru) |
ES (1) | ES2613039T3 (ru) |
SG (1) | SG188420A1 (ru) |
WO (1) | WO2012037937A2 (ru) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3828278A1 (en) | 2012-06-27 | 2021-06-02 | Rutgers, the State University of New Jersey | Rapid assays for t-cell activation by rna measurements using flow cytometry |
AU2013349706B2 (en) * | 2012-11-21 | 2019-08-22 | Assistance Publique - Hopitaux De Paris | Methods for determining the risk of acute graft versus host disease |
WO2016096914A1 (en) * | 2014-12-16 | 2016-06-23 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods for testing the presence of protective antigen-induced memory cd8+ t cells |
US11567065B2 (en) | 2015-06-24 | 2023-01-31 | Thomas Jefferson University | Measuring frequency of pathogen-specific T cells in peripheral blood |
CN105548577B (zh) * | 2016-02-04 | 2017-09-19 | 安徽医科大学 | 一种琥珀酰‑β‑环糊精修饰的莱姆病检测用蛋白质芯片及其制备与应用 |
DE102017121046B4 (de) * | 2017-09-12 | 2023-07-06 | Liv Bode | Verfahren zum Nachweis akuter Borna Disease Virus (BDV) Infektionen und eines diagnostischen Kits hierfür, insbesondere in Kombination mit Verfahren zur Unterscheidung akuter von chronischen und ruhenden BDV-Infektionen und diagnostische Kits hierfür |
EP3785029A1 (en) * | 2018-04-27 | 2021-03-03 | Advaxis, Inc. | Compositions and methods for evaluating potency of listeria-based immunotherapeutics |
US20220056528A1 (en) * | 2018-12-20 | 2022-02-24 | Mikrogen Gmbh | Method of detecting infection with pathogens causing tuberculosis |
EP4168585A1 (en) | 2020-06-19 | 2023-04-26 | Mikrogen GmbH | Method of detecting infection with pathogens causing tuberculosis |
WO2022240887A1 (en) * | 2021-05-10 | 2022-11-17 | Icahn School Of Medicine At Mount Sinai | Methods for detecting and staging cellular viral immune responses |
CN113406334A (zh) * | 2021-06-02 | 2021-09-17 | 浙江省人民医院 | 一种dlbcl相关的生物标记组合物及其应用、dlbcl预后的效果预测模型 |
CN113564159A (zh) * | 2021-07-22 | 2021-10-29 | 山东大学 | 一种分子信标探针、试剂盒及其体外转录荧光定量实时示踪检测方法和应用 |
CN114214456A (zh) * | 2021-12-03 | 2022-03-22 | 重庆医科大学附属儿童医院 | 一种鉴别诊断ebv感染细胞亚型的方法及其应用 |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DK104093D0 (da) * | 1993-09-17 | 1993-09-17 | Osteometer A S | Fremgangsmaade til bestemmelse af collagen-fragmenter i legemsvaesker, test-kit og midler til udoevelse af fremgangsmaaden og anvendelse af fremgangsmaaden til diagnosticering af lidelser associeret til collagen-metabolismen |
US20020193296A1 (en) * | 1997-02-25 | 2002-12-19 | Jiangchun Xu | Compositions and methods for the therapy and diagnosis of prostate cancer |
EP1168924A4 (en) * | 1999-03-31 | 2002-09-04 | Univ Pittsburgh | DERIVATIVED IMMUNOGENES IN VITRO INDUCTION OF ANTIQUE-SPECIFIC T-CELLS USING DENDRITIC CELL-TUMOR CELL OR DENDRITIC CELL-VIRAL CELL |
WO2003012061A2 (en) * | 2001-08-01 | 2003-02-13 | Coley Pharmaceutical Gmbh | Methods and compositions relating to plasmacytoid dendritic cells |
DE10164819A1 (de) * | 2001-11-20 | 2005-05-19 | Deml, Ludwig, Dr. | Verfahren zur Identifizierung von Zielepitopen der T-Zell-vermittelten Immunantwort und zum Nachweis Epitop-spezifischer T-Zellen |
JP2005527506A (ja) * | 2002-02-28 | 2005-09-15 | インターツェル・アクチェンゲゼルシャフト | リガンドe.g.t細胞エピトープの単離方法 |
PL373119A1 (en) * | 2002-05-08 | 2005-08-08 | Northwest Biotherapeutics, Inc. | Quality assays for antigen presenting cells |
EP2295588B1 (en) | 2004-05-27 | 2018-03-07 | The Trustees Of The University Of Pennsylvania | Novel artificial antigen presenting cells and uses thefor |
EP2703499A1 (en) * | 2005-06-02 | 2014-03-05 | Fluidigm Corporation | Analysis using microfluidic partitioning devices to generate single cell samples |
NZ619576A (en) | 2006-12-27 | 2014-07-25 | Harvard College | Compositions and methods for the treatment of infections and tumors |
SI2121920T1 (sl) | 2007-03-01 | 2011-12-30 | Symphogen As | Postopek za kloniranje sorodnih protiteles |
PT2856876T (pt) * | 2007-03-30 | 2018-03-28 | Memorial Sloan Kettering Cancer Center | Expressão constitutiva de ligantes co-estimulatórios em linfócitos t adotivamente transferidos |
JP2009195142A (ja) * | 2008-02-20 | 2009-09-03 | Institute Of Physical & Chemical Research | 肝臓卵形細胞の取得方法 |
AU2009228215B2 (en) | 2008-03-27 | 2015-12-10 | Asterias Biotherapeutics, Inc. | Differentiation of primate pluripotent stem cells to hematopoietic lineage cells |
JP5060413B2 (ja) | 2008-07-15 | 2012-10-31 | 株式会社東芝 | 半導体記憶装置 |
JP5652783B2 (ja) * | 2008-09-08 | 2015-01-14 | 独立行政法人理化学研究所 | NKT細胞由来iPS細胞およびそれ由来のNKT細胞 |
JP5706913B2 (ja) * | 2009-12-16 | 2015-04-22 | 日立化成株式会社 | 攻撃的及び防御的免疫マーカーのexvivo誘導により宿主免疫機能を特徴付けるための方法 |
US20120004124A1 (en) * | 2009-12-21 | 2012-01-05 | Joachim Schultze | Markers of regulatory t cell activation |
-
2010
- 2010-09-17 DE DE102010037622A patent/DE102010037622B4/de not_active Expired - Fee Related
-
2011
- 2011-09-16 WO PCT/DE2011/075225 patent/WO2012037937A2/de active Application Filing
- 2011-09-16 ES ES11808564.6T patent/ES2613039T3/es active Active
- 2011-09-16 CN CN201180044913.8A patent/CN103109191B/zh not_active Expired - Fee Related
- 2011-09-16 SG SG2013016522A patent/SG188420A1/en unknown
- 2011-09-16 JP JP2013528513A patent/JP2013539652A/ja active Pending
- 2011-09-16 EP EP11808564.6A patent/EP2616816B1/de active Active
- 2011-09-16 EA EA201370070A patent/EA201370070A1/ru unknown
- 2011-09-16 BR BR112013006240A patent/BR112013006240A2/pt not_active IP Right Cessation
- 2011-09-16 US US13/821,537 patent/US9733246B2/en active Active
-
2016
- 2016-08-01 JP JP2016151221A patent/JP6508683B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
ES2613039T3 (es) | 2017-05-22 |
JP2013539652A (ja) | 2013-10-28 |
WO2012037937A3 (de) | 2012-06-14 |
CN103109191A (zh) | 2013-05-15 |
WO2012037937A2 (de) | 2012-03-29 |
US9733246B2 (en) | 2017-08-15 |
DE102010037622B4 (de) | 2012-07-12 |
BR112013006240A2 (pt) | 2019-09-24 |
JP2016185170A (ja) | 2016-10-27 |
CN103109191B (zh) | 2016-04-13 |
JP6508683B2 (ja) | 2019-05-08 |
EP2616816A2 (de) | 2013-07-24 |
SG188420A1 (en) | 2013-05-31 |
EP2616816B1 (de) | 2016-11-02 |
US20130288229A1 (en) | 2013-10-31 |
DE102010037622A1 (de) | 2012-03-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EA201370070A1 (ru) | СПОСОБ ДЕТЕКЦИИ, ДИФФЕРЕНЦИАЦИИ И КВАНТИФИКАЦИИ ПОПУЛЯЦИЙ Т-КЛЕТОК С ИСПОЛЬЗОВАНИЕМ КОЛИЧЕСТВЕННОЙ ПЦР С ОБРАТНОЙ ТРАНСКРИПЦИЕЙ В РЕАЛЬНОМ ВРЕМЕНИ (ОТ-колПЦР РВ) | |
EA201600085A1 (ru) | Набор для обнаружения соевого события syht0h2 | |
EP4249605A3 (en) | Methods for analyte detection | |
WO2014186761A3 (en) | Methods for determining responsiveness to an anti-cd47 agent | |
NZ594751A (en) | Method of diagnosis of infection by mycobacteria and reagents therefor | |
UA116618C2 (uk) | СКОНСТРУЙОВАНЕ НА ОСНОВІ ЦИСТЕЇНУ АНТИТІЛО ПРОТИ CD79b | |
PE20141450A1 (es) | Ensayo de lisiloxidasa-homologo 2 y metodos de uso del mismo | |
EA201490999A1 (ru) | Способ проведения количественных анализов | |
WO2011091007A3 (en) | Cell counting and sample chamber and methods of fabrication | |
BR112016008970A2 (pt) | Método de ativação ex vivo e para avaliar ativação plasmática em um indivíduo, ensaio ex vivo para determinar atividade de calicreína plasmática em uma amostra | |
NZ701866A (en) | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples | |
WO2009060847A1 (ja) | 核酸増幅用サンプルの調製方法及び調製キット | |
MX2013013986A (es) | Deteccion rapida de actividad metabolica. | |
CA2855971C (en) | Methods for detecting human papillomavirus and providing prognosis for head and neck squamous cell carcinoma | |
WO2012151105A3 (en) | Basophil analysis system and method | |
ATE504002T1 (de) | Erkennung und vorhersage von frühgeburten | |
EA201500080A1 (ru) | Способ in vitro диагностирования и мониторинга рака | |
HK1126274A1 (en) | T cell assays t | |
BR122019018448B8 (pt) | método para extração de succinilacetona e um ou mais analitos biológicos adicionais | |
RU2013118341A (ru) | Получение сыворотки | |
ATE489632T1 (de) | Antigennachweis | |
RU2014122607A (ru) | Способы определения восприимчивости пациента к внутрибольничной инфекции и составления прогноза развития септического синдрома | |
FR3034523B1 (fr) | Nouvelle methode pour detecter la presence de bacteries productrices de beta-lactamases. | |
IN2014DN06516A (ru) | ||
BR112012028283A2 (pt) | método de determinação de tolerância a transplante de rim |