DK547185D0 - Vektorer til ekspression af hirudin, transformerede celler og fremgangsmaade til fremstilling af hirudin - Google Patents

Vektorer til ekspression af hirudin, transformerede celler og fremgangsmaade til fremstilling af hirudin

Info

Publication number: DK547185D0
Authority: DK; Denmark
Prior art keywords: hirudin; expression; vectors; procedures; preparation
Prior art date: 1984-03-27

Application number

DK547185A

Other languages

English (en)

Other versions

DK175217B1 (da

DK547185A (da

Inventor

Paul Tolstoshev

Richard Harvey

Michael Courtney

Jean-Pierre Lecocq

Original Assignee

Transgene Sa

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-03-27

Filing date

1985-11-26

Publication date

1985-11-26

1984-03-27 Priority claimed from FR8404755A external-priority patent/FR2562088B1/fr

1984-08-27 Priority claimed from FR8413250A external-priority patent/FR2569420B2/fr

1985-11-26 Application filed by Transgene Sa filed Critical Transgene Sa

1985-11-26 Publication of DK547185D0 publication Critical patent/DK547185D0/da

1985-11-26 Publication of DK547185A publication Critical patent/DK547185A/da

2004-07-12 Application granted granted Critical

2004-07-12 Publication of DK175217B1 publication Critical patent/DK175217B1/da

Links

239000013598 vector Substances 0.000 title abstract 2
102000007625 Hirudins Human genes 0.000 title 2
108010007267 Hirudins Proteins 0.000 title 2
WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical compound C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 title 2
229940006607 hirudin Drugs 0.000 title 2
238000000034 method Methods 0.000 title 1
108090000623 proteins and genes Proteins 0.000 abstract 3
108020004705 Codon Proteins 0.000 abstract 2
238000010367 cloning Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Biochemistry (AREA)
Biophysics (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Biomedical Technology (AREA)
Tropical Medicine & Parasitology (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Gastroenterology & Hepatology (AREA)
Microbiology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Medicinal Chemistry (AREA)
Peptides Or Proteins (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

DK198505471A 1984-03-27 1985-11-26 Hirudinanalog, vektorer til ekspression af hirudinanalog, transformerede celler og fremgangsmåde til fremstilling af hirudinanalog DK175217B1 (da)

Applications Claiming Priority (6)

Application Number	Priority Date	Filing Date	Title
FR8404755A FR2562088B1 (fr)	1984-03-27	1984-03-27	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
FR8404755		1984-03-27
FR8413250A FR2569420B2 (fr)	1984-08-27	1984-08-27	Vecteur d'expression de l'hirudine, procede pour sa preparation par culture de cellules transformees et hirudine obtenue
FR8413250		1984-08-27
PCT/FR1985/000062 WO1985004418A1 (fr)	1984-03-27	1985-03-27	Vecteurs d'expression de l'hirudine, cellules transformees et procede de preparation de l'hirudine
FR8500062		1985-03-27

Publications (3)

Publication Number	Publication Date
DK547185D0 true DK547185D0 (da)	1985-11-26
DK547185A DK547185A (da)	1985-11-26
DK175217B1 DK175217B1 (da)	2004-07-12

Family

ID=26223885

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK198505471A DK175217B1 (da)	1984-03-27	1985-11-26	Hirudinanalog, vektorer til ekspression af hirudinanalog, transformerede celler og fremgangsmåde til fremstilling af hirudinanalog

Country Status (7)

Country	Link
US (1)	US5824505A (da)
EP (1)	EP0158564B1 (da)
AT (1)	ATE78294T1 (da)
CA (2)	CA1341414C (da)
DE (1)	DE3586333T2 (da)
DK (1)	DK175217B1 (da)
WO (1)	WO1985004418A1 (da)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3438296A1 (de) *	1984-04-18	1985-11-07	Hoechst Ag, 6230 Frankfurt	Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel
EP0168342B1 (de) *	1984-06-14	1991-07-03	Ciba-Geigy Ag	Verfahren zur Herstellung von Thrombin-Inhibitoren
DE3429430A1 (de) *	1984-08-10	1986-02-20	Hoechst Ag, 6230 Frankfurt	Gentechnologisches verfahren zur herstellung von hirudin und mittel zur durchfuehrung dieses verfahrens
DE3445517C2 (de) *	1984-12-13	1993-11-18	Ciba Geigy	Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins
DE3506992A1 (de) *	1985-02-27	1986-08-28	Plantorgan Werk Heinrich G.E. Christensen, KG, 2903 Bad Zwischenahn	Modifizierte hirudine, verfahren zu deren herstellung und pharmazeutische mittel, die diese wirkstoffe enthalten
FR2593518B1 (fr) *	1985-05-02	1989-09-08	Transgene Sa	Vecteurs d'expression et de secretion de l'hirudine par les levures transformees
EP0209061B1 (de) *	1985-07-17	1994-01-12	Hoechst Aktiengesellschaft	Neue Polypeptide mit blutgerinnungshemmender Wirkung, Verfahren zu deren Herstellung bzw. Gewinnung, deren Verwendung und diese enthaltende Mittel
DE3541856A1 (de) *	1985-11-27	1987-06-04	Hoechst Ag	Eukaryotische fusionsproteine, ihre herstellung und verwendung sowie mittel zur durchfuehrung des verfahrens
GB8530631D0 (en)	1985-12-12	1986-01-22	Ciba Geigy Ag	Thrombin inhibitors
MY101203A (en) *	1985-12-12	1991-08-17	Ucp Gen Pharma Ag	Production of thrombin iinhibitors.
MC1834A1 (fr) *	1986-07-10	1988-06-03	Transgene Sa	Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation
FR2607516B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs perfectionnes d'expression d'un variant de l'hirudine dans les levures, procede et produit obtenu
FR2618159B2 (fr) *	1987-07-15	1990-02-02	Transgene Sa	Procede de stabilisation d'un plasmide contenu dans une souche bacterienne et souche obtenue
DK408687A (da) *	1986-08-05	1988-02-06	Transgene Sa	Fremgangsmaade til stabilisering af et plasmid
FR2607517B2 (fr) *	1986-12-01	1989-12-22	Transgene Sa	Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
US5256559A (en) *	1988-03-04	1993-10-26	Biogen, Inc.	Methods and compositions for inhibiting platelet aggregation
FR2628429B1 (fr) *	1988-03-08	1990-12-28	Transgene Sa	Variants de l'hirudine, leurs utilisations et les procedes pour les obtenir
DE3819079A1 (de) *	1988-06-04	1989-12-07	Hoechst Ag	Hirudin-derivate mit verzoegerter wirkung
DE3835815A1 (de) *	1988-10-21	1990-04-26	Hoechst Ag	Neue isohirudine
FR2646437B1 (fr) *	1989-04-28	1991-08-30	Transgene Sa	Nouvelles sequences d'adn, leur application en tant que sequence codant pour un peptide signal pour la secretion de proteines matures par des levures recombinantes, cassettes d'expression, levures transformees et procede de preparation de proteines correspondant
US5179196A (en) *	1989-05-04	1993-01-12	Sri International	Purification of proteins employing ctap-iii fusions
DE4009268A1 (de) *	1990-03-22	1991-09-26	Consortium Elektrochem Ind	Sekretion von hirudinderivaten
US6514730B1 (en)	1991-03-21	2003-02-04	Consortium für elektrochemische Industrie GmbH	Secretion of hirudin derivatives
US5837808A (en) *	1991-08-20	1998-11-17	Baxter International Inc.	Analogs of hirudin
DE4140381A1 (de) *	1991-12-07	1993-06-09	Hoechst Ag, 6230 Frankfurt, De	Neue synthetische isohirudine mit verbesserter stabilitaet
HU214698B (hu) *	1992-04-09	1998-06-29	Gyógyszerkutató Intézet Kft.	Eljárás rekombináns deszulfátó-hirudin HV-1 peptidek előállítására
RU2183214C2 (ru) *	1992-12-04	2002-06-10	Шеринг Аг	Природный и рекомбинантный ингибиторы тромбина, их получение и применение
GB9401447D0 (en)	1994-01-26	1994-03-23	Ciba Geigy Ag	Pharmaceutical compositions
DE4404168A1 (de) *	1994-02-10	1995-08-17	Hoechst Ag	Hirudinderivate und Verfahren zu deren Herstellung
DE19529997C1 (de)	1995-08-16	1997-04-10	Hoechst Ag	Verfahren zur Inaktivierung von Carboxypeptidase Y in hirudinhaltigen Kulturbrühen
GB2304048A (en) *	1995-08-12	1997-03-12	John William Carson	Medicament containing saliva extract
DE19543737A1 (de) *	1995-11-24	1997-05-28	Hoechst Ag	Verfahren zur Ultrafiltration von Peptide oder Proteine enthaltender biologischer Matrices
DE19544233A1 (de)	1995-11-28	1997-06-05	Hoechst Ag	Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
DE69624087T2 (de)	1996-01-31	2003-06-05	Sumitomo Bakelite Co	Verfahren zur Herstellung von in Epoxyharz eingekapselter Halbleitervorrichtung
DE19607239A1 (de)	1996-02-27	1997-08-28	Behringwerke Ag	Pharmazeutische Zusammensetzung, enthaltend Hirudin und Verfahren zu deren Herstellung
AR027686A1 (es) *	2000-03-20	2003-04-09	Knoll Ag	Uso de sustancias inhibidoras de la coagulacion en el tratamiento extracorporeo de la sangre
US7638618B2 (en)	2001-02-20	2009-12-29	Sanofi-Aventis Deutschland Gmbh	Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest
US7202059B2 (en) *	2001-02-20	2007-04-10	Sanofi-Aventis Deutschland Gmbh	Fusion proteins capable of being secreted into a fermentation medium
DE10108212A1 (de) *	2001-02-20	2002-08-22	Aventis Pharma Gmbh	Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
DE10108211A1 (de) *	2001-02-20	2002-08-22	Aventis Pharma Gmbh	Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen
NZ527590A (en)	2001-03-09	2005-12-23	Genentech Inc	Process for production of polypeptides
US7795205B2 (en) *	2004-04-12	2010-09-14	Canyon Pharmaceuticals, Inc.	Methods for effecting regression of tumor mass and size in a metastasized pancreatic tumor
US20110034396A1 (en) *	2005-09-28	2011-02-10	Biovascular, Inc.	Methods and compositions for inhibiting cell migration and treatment of inflammatory conditions
US7691839B2 (en)	2005-09-28	2010-04-06	Biovascular, Inc.	Methods and compositions for blocking platelet and cell adhesion, cell migration and inflammation
CN102262161B (zh) *	2011-08-16	2013-09-11	天津市百利康泰生物技术有限公司	水蛭素在特定条件下失活后检测凝血四项方面的应用
CN116987181B (zh) *	2023-09-27	2023-12-08	北京元延医药科技股份有限公司	高生物学活性的天然水蛭素和高收率制备它们的方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4517294A (en) *	1982-03-03	1985-05-14	Genentech, Inc.	Human antithrombin III
FR2526661B1 (fr) *	1982-05-13	1986-02-21	Transgene Sa	Nouveaux vecteurs d'expression de la proteine antigenique de la rage et leur application a la preparation de vaccins
US4578355A (en) *	1983-01-12	1986-03-25	The United States Of America As Represented By The Department Of Health And Human Services	Plasmid cloning vector pAS1
EP0168342B1 (de) *	1984-06-14	1991-07-03	Ciba-Geigy Ag	Verfahren zur Herstellung von Thrombin-Inhibitoren
US5232912A (en) *	1988-12-07	1993-08-03	Merrell Dow Pharmaceuticals Inc.	Anticoagulant peptides
ES2129749T3 (es) *	1990-11-08	1999-06-16	Japan Energy Corp	Vector de secrecion, microorganismos transformados que contienen el indicado vector y produccion de un producto a partir de dicho microorganismo.

1985
- 1985-03-27 CA CA000477676A patent/CA1341414C/fr not_active Expired - Fee Related
- 1985-03-27 AT AT85400598T patent/ATE78294T1/de not_active IP Right Cessation
- 1985-03-27 EP EP85400598A patent/EP0158564B1/fr not_active Expired - Lifetime
- 1985-03-27 CA CA000615690A patent/CA1341417C/fr not_active Expired - Fee Related
- 1985-03-27 DE DE8585400598T patent/DE3586333T2/de not_active Expired - Lifetime
- 1985-03-27 WO PCT/FR1985/000062 patent/WO1985004418A1/fr unknown
- 1985-11-26 DK DK198505471A patent/DK175217B1/da not_active IP Right Cessation
1995
- 1995-07-10 US US08/500,111 patent/US5824505A/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
WO1985004418A1 (fr)	1985-10-10
CA1341414C (fr)	2002-12-31
CA1341417C (fr)	2003-01-21
ATE78294T1 (de)	1992-08-15
DE3586333T2 (de)	1992-12-10
EP0158564A1 (fr)	1985-10-16
EP0158564B1 (fr)	1992-07-15
DE3586333D1 (de)	1992-08-20
DK175217B1 (da)	2004-07-12
DK547185A (da)	1985-11-26
US5824505A (en)	1998-10-20

Legal Events

Date	Code	Title	Description
2005-04-11	PUP	Patent expired

Publication	Publication Date	Title
DK547185D0 (da)	1985-11-26	Vektorer til ekspression af hirudin, transformerede celler og fremgangsmaade til fremstilling af hirudin
UA27706C2 (uk)	2000-10-16	Спосіб одержання гетерологічного поліпептиду
DK188582A (da)	1982-10-30	Bacillus-kloningsvektorer rekombinations-dna-molekyler bacillus-vaerter transformeret dermed samt fremgangsmaader til ekspressionaf fremmede dna-sekvenser og fremstilling af polypeptideer som er kodede dermed
WO1994003616A3 (fr)	1994-03-31	Vecteur de clonage et/ou de sequençage
ES8308361A1 (es)	1983-08-16	Un metodo para la produccion de un polipeptido.
DK481383A (da)	1984-04-20	Syntetisk mutein af et biologisk aktivt protein, gen kodende for samme, vektor omfattende dette gen, vaertcelle transformeret med denne vektor samt fremgangsmaader til fremstilling af muteinet og genet kodende herfor
DK95197A (da)	1997-08-20	Fremgangsmåde til fremstilling af rekombinant humant erythropoietin
ZA839231B (en)	1984-08-29	Molecular cloning and characterization of a further gene sequence coding for human relaxin
DK166357C (da)	1993-09-06	Partikler med immunogene egenskaber svarende til hbs antigen samt vektorer og animalske celler til produktion af saadanne partikler
GB8424454D0 (en)	1984-10-31	Recombinant dna cloning vectors
ZA859437B (en)	1987-08-26	Dna sequence encoding a hirudin-like protein and process for preparing such protein
YU49126B (sh)	2004-03-12	Varijantni cd44-površinski proteini, dna-sekvence koje ih kodiraju i antitela protiv ovih proteina
DK432987A (da)	1987-08-19	Fremgangsmaade til forbedring af ekspressionen af eukaryotprotein
SE8101665L (sv)	1981-09-18	Polypeptidframstellning
DE59109072D1 (de)	1999-01-07	Klonierung der N-Methylhydantoinase
EP0162319A3 (en)	1987-12-02	Method of linking heterogenes
NO175872C (no)	1994-12-21	Plasmidvektor, fremgangsmåte for fremstilling av peptider og myelomcelle
DK447083A (da)	1984-03-31	Dna, som koder for humant interferon-gamma, rekombinant-dna indeholdende denne dna, mikroorganisme indeholdende rekombinant-dna'en og fremgangsmaade til fremstilling af humant interferon-gamma-polypeptid
DK0385391T3 (da)	2000-05-29	Ekspressionsvektorer til syntese af proteiner i Schizosaccharomyces pombe
ES8800353A1 (es)	1987-11-01	Procedimiento de clonacion de factor humano viii-r.
DK391185A (da)	1986-03-01	Rekombinant saccharomyces
DK454286A (da)	1987-03-28	Fremgangsmaade til stabil ekspression af en fremmed polypeptidkodendesekvens samt hermed transformerede e. coli-celler
ATE107694T1 (de)	1994-07-15	Für a.nidulans-isopenicillin-n-synthetase codierende dns, deren verwendung und dafür codierende vektoren.
IT8249433A0 (it)	1982-11-05	Procedimento per la produzione di un polipeptide eterologo mediante espressione di un vettore di clonazione ricombinante, vettore impiegato e microrganismo ospite cosi' trasformato
ATE52805T1 (de)	1990-06-15	Psm112-plasmidvektor zur expression in bacillus subtilis.