DK3660146T3 - Ydelsesforbedrede og opbevaringsstabile proteasevarianter - Google Patents

Ydelsesforbedrede og opbevaringsstabile proteasevarianter Download PDF

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Publication number
DK3660146T3
DK3660146T3 DK18209177.7T DK18209177T DK3660146T3 DK 3660146 T3 DK3660146 T3 DK 3660146T3 DK 18209177 T DK18209177 T DK 18209177T DK 3660146 T3 DK3660146 T3 DK 3660146T3
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DK
Denmark
Prior art keywords
enhanced
performance
storage
protease variants
stable protease
Prior art date
Application number
DK18209177.7T
Other languages
English (en)
Inventor
Layla Fernandez
Sabine Griemert
Nina Mussmann
Inken Prueser
Daria Strauss
Christian Degering
Susanne Wieland
Original Assignee
Henkel Ag & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel Ag & Co Kgaa filed Critical Henkel Ag & Co Kgaa
Application granted granted Critical
Publication of DK3660146T3 publication Critical patent/DK3660146T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1024In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21062Subtilisin (3.4.21.62)

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK18209177.7T 2018-11-29 2018-11-29 Ydelsesforbedrede og opbevaringsstabile proteasevarianter DK3660146T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP18209177.7A EP3660146B1 (de) 2018-11-29 2018-11-29 Leistungsverbesserte und lagerstabile protease-varianten

Publications (1)

Publication Number Publication Date
DK3660146T3 true DK3660146T3 (da) 2024-02-19

Family

ID=64559554

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18209177.7T DK3660146T3 (da) 2018-11-29 2018-11-29 Ydelsesforbedrede og opbevaringsstabile proteasevarianter

Country Status (5)

Country Link
US (1) US11746341B2 (da)
EP (1) EP3660146B1 (da)
DK (1) DK3660146T3 (da)
ES (1) ES2967424T3 (da)
PL (1) PL3660146T3 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102020205381A1 (de) * 2020-04-29 2021-11-04 Henkel Ag & Co. Kgaa Hochalkalisches Textilwaschmittel mit Protease
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
DE102022205588A1 (de) * 2022-06-01 2023-12-07 Henkel Ag & Co. Kgaa Wasch- und reinigungsmittel mit verbesserter enzymstabilität
WO2024094733A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094732A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094735A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
EP1921148B1 (en) 1994-02-24 2011-06-08 Henkel AG & Co. KGaA Improved enzymes and detergents containing them
DE102004027091A1 (de) * 2004-06-02 2005-12-29 Henkel Kgaa Leistungsverbesserte Alkalische Protease-Varianten und Wasch- und Reinigungsmittel, enthaltend diese leistungsverbesserten Alkalische Protease-Varianten
DE102008017103A1 (de) 2008-04-02 2009-10-08 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel enthaltend Proteasen aus Xanthomonas
DE102009029513A1 (de) 2009-09-16 2011-03-24 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Proteasen
DE102010063458A1 (de) 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Amylase
DE102011118021A1 (de) 2011-10-28 2013-05-02 Henkel Ag & Co. Kgaa Leistungsverbesserte und temperaturstabile Proteasevarianten
DE102012201522A1 (de) * 2012-02-02 2013-08-08 Basf Se Lagerstabiles flüssiges Geschirrspülmittel enthaltend Protease und Amylase
DE102013218253A1 (de) 2013-09-12 2015-03-12 Henkel Ag & Co. Kgaa Festes Textilwaschmittel mit verbesserter Proteaseleistung
DE102013219467A1 (de) 2013-09-26 2015-03-26 Henkel Ag & Co. Kgaa Stabilisierte Inhibitor-Proteasevarianten
DE102013221206A1 (de) 2013-10-18 2015-04-23 Henkel Ag & Co. Kgaa Proteasevarianten mit erhöhter Stabilität
US20170175098A1 (en) * 2014-03-28 2017-06-22 Novozymes A/S Enzyme Variants and Polynucleotides Encoding Same
US10647947B2 (en) * 2014-06-04 2020-05-12 Novozymes A/S Detergent composition
WO2016001449A1 (en) * 2014-07-04 2016-01-07 Novozymes A/S Subtilase variants and polynucleotides encoding same
CA2963148C (en) * 2014-10-28 2024-04-30 Agrivida, Inc. Methods and compositions for stabilizing trans-splicing intein modified proteases
WO2016096714A1 (en) 2014-12-15 2016-06-23 Henkel Ag & Co. Kgaa Detergent composition comprising subtilase variants
CN109642222A (zh) * 2016-07-13 2019-04-16 诺维信公司 食物芽孢杆菌dna酶变体
WO2018069158A1 (en) * 2016-10-11 2018-04-19 Basf Se Protease resistant to natural inhibitors

Also Published As

Publication number Publication date
ES2967424T3 (es) 2024-04-30
US11746341B2 (en) 2023-09-05
EP3660146B1 (de) 2023-11-15
EP3660146A1 (de) 2020-06-03
PL3660146T3 (pl) 2024-03-18
US20200172890A1 (en) 2020-06-04

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