DK3219796T3 - Fremgangsmåder til kontrol af strømningen i metaboliske reaktionsveje ved hjælp af proteasemanipulation - Google Patents

Fremgangsmåder til kontrol af strømningen i metaboliske reaktionsveje ved hjælp af proteasemanipulation Download PDF

Info

Publication number
DK3219796T3
DK3219796T3 DK17163812.5T DK17163812T DK3219796T3 DK 3219796 T3 DK3219796 T3 DK 3219796T3 DK 17163812 T DK17163812 T DK 17163812T DK 3219796 T3 DK3219796 T3 DK 3219796T3
Authority
DK
Denmark
Prior art keywords
protease
manipulation
procedures
monitoring flow
metabolic reactions
Prior art date
Application number
DK17163812.5T
Other languages
English (en)
Inventor
James R Swartz
Original Assignee
Greenlight Biosciences Inc
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Greenlight Biosciences Inc, Univ Leland Stanford Junior filed Critical Greenlight Biosciences Inc
Application granted granted Critical
Publication of DK3219796T3 publication Critical patent/DK3219796T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/305—Pyrimidine nucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21009—Enteropeptidase (3.4.21.9), i.e. enterokinase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DK17163812.5T 2010-08-31 2011-08-31 Fremgangsmåder til kontrol af strømningen i metaboliske reaktionsveje ved hjælp af proteasemanipulation DK3219796T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37882810P 2010-08-31 2010-08-31
EP11752452.0A EP2611922A1 (en) 2010-08-31 2011-08-31 Methods for control of flux in metabolic pathways through protease manipulation

Publications (1)

Publication Number Publication Date
DK3219796T3 true DK3219796T3 (da) 2020-12-21

Family

ID=44658836

Family Applications (1)

Application Number Title Priority Date Filing Date
DK17163812.5T DK3219796T3 (da) 2010-08-31 2011-08-31 Fremgangsmåder til kontrol af strømningen i metaboliske reaktionsveje ved hjælp af proteasemanipulation

Country Status (9)

Country Link
US (2) US8916358B2 (da)
EP (2) EP2611922A1 (da)
JP (2) JP6280367B2 (da)
KR (1) KR101988063B1 (da)
CA (1) CA2809284C (da)
DK (1) DK3219796T3 (da)
ES (1) ES2839424T3 (da)
IL (1) IL224637A (da)
WO (1) WO2012030980A1 (da)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2376619A4 (en) 2008-12-15 2012-07-04 Greenlight Biosciences Inc METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS
EP2379728A4 (en) * 2008-12-22 2016-04-13 Greenlight Biosciences Inc COMPOSITIONS AND METHOD FOR PRODUCING A COMPOUND
JP2013526277A (ja) * 2010-05-07 2013-06-24 グリーンライト バイオサイエンシーズ インコーポレーテッド 酵素リロケーションにより代謝経路のフラックスを制御する方法
EP2611922A1 (en) 2010-08-31 2013-07-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
CN104093848A (zh) 2011-09-09 2014-10-08 绿光生物科学公司 碳青霉烯(carbapenem)的无细胞制备
CA2896079A1 (en) 2012-12-21 2014-06-26 Greenlight Biosciences, Inc. Cell-free system for converting methane into fuel and chemical compounds
EP3460069B1 (en) 2013-08-05 2021-10-20 Greenlight Biosciences, Inc. Therapeutically active compounds and their methods of use
EP4148127A1 (en) 2014-06-11 2023-03-15 Duke University Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
EP3277808A4 (en) * 2015-03-30 2019-05-22 Greenlight Biosciences, Inc. ACELLULAR PRODUCTION OF RIBONUCLEIC ACID
WO2016196233A1 (en) * 2015-05-30 2016-12-08 Genomatica, Inc. Vinylisomerase-dehydratases, alkenol dehydratases, linalool dehydratases and/ crotyl alcohol dehydratases and methods for making and using them
MY195729A (en) 2016-04-06 2023-02-07 Greenlight Biosciences Inc Cell-Free Production of Ribonucleic Acid
AU2018205503A1 (en) 2017-01-06 2019-07-25 Greenlight Biosciences, Inc. Cell-free production of sugars
KR102357193B1 (ko) 2017-02-21 2022-02-04 듀크 유니버시티 견고한 동적 대사 조절을 위한 조성물 및 방법
MX2020003841A (es) 2017-10-11 2020-11-06 Greenlight Biosciences Inc Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos.
US11541105B2 (en) 2018-06-01 2023-01-03 The Research Foundation For The State University Of New York Compositions and methods for disrupting biofilm formation and maintenance
WO2019246488A1 (en) 2018-06-21 2019-12-26 Duke University Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control
WO2020132027A2 (en) 2018-12-21 2020-06-25 Greenlight Biosciences, Inc. Cell-free production of allulose

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3223592A (en) 1957-09-27 1965-12-14 Yamasa Shoyu Kk Production of 5'-nucleotides
JPS4841555B1 (da) 1969-08-04 1973-12-07
USRE28886E (en) 1969-08-04 1976-06-29 Kyowa Hakko Kogyo Co., Ltd. Method for preparing cytidine diphosphate choline
US4006060A (en) 1974-11-25 1977-02-01 Merck & Co., Inc. Thienamycin production
US3950357A (en) 1974-11-25 1976-04-13 Merck & Co., Inc. Antibiotics
US4194047A (en) 1975-11-21 1980-03-18 Merck & Co., Inc. Substituted N-methylene derivatives of thienamycin
US4266034A (en) 1978-04-14 1981-05-05 Exxon Research And Engineering Company Method for producing microbial cells and use thereof to produce oxidation products
US4248966A (en) 1979-05-17 1981-02-03 Massachusetts Institute Of Technology Synthesis of isopenicillin derivatives in the absence of living cells
US4270537A (en) 1979-11-19 1981-06-02 Romaine Richard A Automatic hypodermic syringe
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4329481A (en) 1980-06-25 1982-05-11 Merck & Co., Inc. Process for the preparation of N-protected N-formimidoyl 2-aminoethanethiol
US4292436A (en) 1980-06-25 1981-09-29 Merck & Co., Inc. Process for the preparation of N-protected N-formimidoyl 2-aminoethanethiol
US4374772A (en) 1981-03-19 1983-02-22 Merck & Co., Inc. Process for the preparation of N-formimidoyl thienamycin and reagents therefor
JPS585189A (ja) 1981-07-01 1983-01-12 Sanraku Inc 新規なアミドヒドロラ−ゼ
US4460689A (en) 1982-04-15 1984-07-17 Merck & Co., Inc. DNA Cloning vector TG1, derivatives, and processes of making
US4596556A (en) 1985-03-25 1986-06-24 Bioject, Inc. Hypodermic injection apparatus
EP0202094B1 (en) 1985-05-13 1988-09-21 Unitika Ltd. Process for producing physiologically active substance
JPS61260895A (ja) 1985-05-13 1986-11-19 Unitika Ltd 生理活性物質の製造方法
US5672497A (en) 1986-03-21 1997-09-30 Eli Lilly And Company Method for increasing the antibiotic-producing ability of antibiotic-producing microorganisms
JPS637788A (ja) 1986-06-25 1988-01-13 Kanegafuchi Chem Ind Co Ltd 補酵素の回収方法
CA1283827C (en) 1986-12-18 1991-05-07 Giorgio Cirelli Appliance for injection of liquid formulations
US4946783A (en) 1987-01-30 1990-08-07 President And Fellows Of Harvard College Periplasmic protease mutants of Escherichia coli
GB8704027D0 (en) 1987-02-20 1987-03-25 Owen Mumford Ltd Syringe needle combination
US4941880A (en) 1987-06-19 1990-07-17 Bioject, Inc. Pre-filled ampule and non-invasive hypodermic injection device assembly
US4790824A (en) 1987-06-19 1988-12-13 Bioject, Inc. Non-invasive hypodermic injection device
US4940460A (en) 1987-06-19 1990-07-10 Bioject, Inc. Patient-fillable and non-invasive hypodermic injection device assembly
US4950603A (en) 1987-11-02 1990-08-21 Eli Lilly And Company Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii
JPH01228473A (ja) 1988-03-08 1989-09-12 Hikari Kimura 新規な組み換え体ｄｎａおよびグルタチオンの製造法
US5339163A (en) 1988-03-16 1994-08-16 Canon Kabushiki Kaisha Automatic exposure control device using plural image plane detection areas
US5070020A (en) 1988-05-09 1991-12-03 Eli Lilly And Company Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase
FR2638359A1 (fr) 1988-11-03 1990-05-04 Tino Dalto Guide de seringue avec reglage de la profondeur de penetration de l'aiguille dans la peau
IL92740A0 (en) 1988-12-22 1990-09-17 Lilly Co Eli Recombinant dna expression vectors and dna compounds that encode isopenicillin n epimerase(racemase)activity
US5064413A (en) 1989-11-09 1991-11-12 Bioject, Inc. Needleless hypodermic injection device
US5312335A (en) 1989-11-09 1994-05-17 Bioject Inc. Needleless hypodermic injection device
CA2034735A1 (en) 1990-01-26 1991-07-27 Barbara J. Weigel Recombinant dna expression and dna compounds that encode isopenicillin n epimerase activity
US5190521A (en) 1990-08-22 1993-03-02 Tecnol Medical Products, Inc. Apparatus and method for raising a skin wheal and anesthetizing skin
US5527288A (en) 1990-12-13 1996-06-18 Elan Medical Technologies Limited Intradermal drug delivery device and method for intradermal delivery of drugs
GB9118204D0 (en) 1991-08-23 1991-10-09 Weston Terence E Needle-less injector
SE9102652D0 (sv) 1991-09-13 1991-09-13 Kabi Pharmacia Ab Injection needle arrangement
KR0177841B1 (ko) 1992-01-30 1999-04-01 나까무라 간노스께 시티딘 디인산 콜린의 제조방법
US5328483A (en) 1992-02-27 1994-07-12 Jacoby Richard M Intradermal injection device with medication and needle guard
US5383851A (en) 1992-07-24 1995-01-24 Bioject Inc. Needleless hypodermic injection device
US5569189A (en) 1992-09-28 1996-10-29 Equidyne Systems, Inc. hypodermic jet injector
US5334144A (en) 1992-10-30 1994-08-02 Becton, Dickinson And Company Single use disposable needleless injector
US5319122A (en) 1992-11-12 1994-06-07 Merck & Co., Inc. Process for the preparation of benzylformimidate
DE69432787D1 (de) * 1993-01-15 2003-07-10 Inst Genetics Llc Klonierung von Enterokinase und Verfahren zu deren Verwendung
US6746859B1 (en) * 1993-01-15 2004-06-08 Genetics Institute, Llc Cloning of enterokinase and method of use
US5436131A (en) 1993-04-02 1995-07-25 Merck & Co., Inc. Color screening assay for identifying inhibitor resistant HIV protease mutants
WO1995024176A1 (en) 1994-03-07 1995-09-14 Bioject, Inc. Ampule filling device
US5466220A (en) 1994-03-08 1995-11-14 Bioject, Inc. Drug vial mixing and transfer device
KR0131166B1 (ko) 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법
GB9410142D0 (en) 1994-05-20 1994-07-06 Univ Warwick Carbapenems
AU4114596A (en) * 1994-12-09 1996-06-26 Novo Nordisk A/S A process of producing extracellular proteins in bacteria
US5599302A (en) 1995-01-09 1997-02-04 Medi-Ject Corporation Medical injection system and method, gas spring thereof and launching device using gas spring
JPH08196284A (ja) 1995-01-19 1996-08-06 Canon Inc 酵素反応素子およびその製造方法、酵素反応器ならびに酵素反応方法
US5730723A (en) 1995-10-10 1998-03-24 Visionary Medical Products Corporation, Inc. Gas pressured needle-less injection device and method
JP3633629B2 (ja) 1995-07-06 2005-03-30 ザ レランド スタンフォード ジュニア ユニバーシティ ポリケチドの無細胞合成
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US5893397A (en) 1996-01-12 1999-04-13 Bioject Inc. Medication vial/syringe liquid-transfer apparatus
GB9607549D0 (en) 1996-04-11 1996-06-12 Weston Medical Ltd Spring-powered dispensing device
KR100205768B1 (en) 1996-08-24 1999-07-01 Choongwae Pharm Co Stereo-selective composition of 4-acetoxyazetidinone
GB9622516D0 (en) 1996-10-29 1997-01-08 Univ Cambridge Tech Enzymic cofactor cycling
AU5960498A (en) 1997-01-14 1998-08-03 Bio-Technical Resources Process for production of (n)-glucosamine
US5993412A (en) 1997-05-19 1999-11-30 Bioject, Inc. Injection apparatus
IT1298087B1 (it) 1998-01-08 1999-12-20 Fiderm S R L Dispositivo per il controllo della profondita' di penetrazione di un ago, in particolare applicabile ad una siringa per iniezioni
US6159693A (en) 1998-03-13 2000-12-12 Promega Corporation Nucleic acid detection
GB9815666D0 (en) 1998-07-17 1998-09-16 Smithkline Beecham Plc Novel compounds
EP1142992A4 (en) 1998-12-24 2003-05-14 Takara Bio Inc POLYPEPTIDES
US6613552B1 (en) 1999-01-29 2003-09-02 Board Of Trustees Operating Michigan State University Biocatalytic synthesis of shikimic acid
US6994986B2 (en) 1999-03-17 2006-02-07 The Board Of Trustees Of The Leland Stanford University In vitro synthesis of polypeptides by optimizing amino acid metabolism
US6168931B1 (en) 1999-03-17 2001-01-02 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of biological macromolecules using a novel ATP regeneration system
EP1177311B1 (en) 1999-03-17 2014-11-12 The Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
WO2000062804A2 (en) 1999-04-15 2000-10-26 The Regents Of The University Of California Identification of sortase gene
US6284483B1 (en) 1999-10-06 2001-09-04 Board Of Trustees Operating Michigan State University Modified synthetases to produce penicillins and cephalosporins under the control of bicarbonate
CA2293852A1 (fr) 1999-12-30 2001-06-30 Purecell Technologies Inc. Procede de preparation d'extraits de plantes actifs et utiles a la capture de radicaux libres; les extraits, et compositions et dispositifs les comprenant
WO2001066705A1 (en) 2000-03-07 2001-09-13 Akzo Nobel N.V. Rna polymerase mutants with increased thermostability
US6548276B2 (en) 2000-09-06 2003-04-15 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
US7041479B2 (en) 2000-09-06 2006-05-09 The Board Of Trustess Of The Leland Stanford Junior University Enhanced in vitro synthesis of active proteins containing disulfide bonds
WO2002081440A2 (en) 2001-04-04 2002-10-17 Genencor International, Inc. Methods for the production of products in host cells
WO2002088325A2 (en) 2001-05-02 2002-11-07 University Of South Florida Vector system for selection of genes encoding secreted proteins and membrane-bound proteins
EP1279736A1 (en) 2001-07-27 2003-01-29 Université de Nantes Methods of RNA and protein synthesis
WO2003027301A1 (fr) 2001-09-06 2003-04-03 Ajinomoto Co., Inc. Procede permettant de produire un alcool au moyen d'un micro-organisme
JP4831932B2 (ja) 2001-09-13 2011-12-07 ジェネンテック， インコーポレイテッド アミノペプチダーゼ
WO2003038117A2 (en) 2001-10-30 2003-05-08 The Board Of Trustees Of The Leland Stanford Junior University Enhanced in vitro nucleic acid synthesis using nucleoside monophosphates
US20040038250A1 (en) 2002-04-04 2004-02-26 Astur-Pharma, S.A. Gene cluster for thienamycin biosynthesis, genetic manipulation and utility
DE10219714A1 (de) 2002-05-02 2003-11-27 Holland Sweetener Co Verfahren zur mikrobielien Herstellung von aromatischen Aminosäuren und anderen Metaboliten des aromatischen Aminosäurebiosyntheseweges
ATE437956T1 (de) 2002-05-08 2009-08-15 Kyowa Hakko Bio Co Ltd Verfahren zur herstellung von cytidin 5'- diphosphatcholin
ES2344552T3 (es) 2002-05-29 2010-08-31 Yamasa Corporation Metodo para producir nucleosido difosfato usando polifosfato: amp fosfotransferasa.
CN101365785A (zh) 2002-07-01 2009-02-11 阿基昂生命科学公司,以生物技术资源部的名义经营 用于生产葡糖胺和n-乙酰氨基葡糖的方法和物质
AU2003259912B2 (en) 2002-08-19 2008-10-23 The Board Of Trustees Of The Leland Stanford Junior University Improved methods of in vitro protein synthesis
CA2501645A1 (en) * 2002-10-10 2004-04-22 Diversa Corporation Proteases, nucleic acids encoding them and methods for making and using them
AU2003295264A1 (en) 2002-12-23 2004-07-14 Rijksuniversiteit Groningen Modulation of the thioredoxin pathway
DK1587947T3 (da) 2003-01-27 2010-05-10 Dsm Ip Assets Bv Fremstilling af 5'-ribonucleotider
AU2004238843B2 (en) 2003-05-09 2009-04-23 Research Development Foundation Insertion of furin protease cleavage sites in membrane proteins and uses thereof
US20080199915A1 (en) 2003-06-06 2008-08-21 Rna-Line Oy Methods and Kits For Mass Production Of Dsrna
US20050054044A1 (en) 2003-07-18 2005-03-10 The Board Of Trustees Of The Leland Stanford Junior University Method of alleviating nucleotide limitations for in vitro protein synthesis
US7341852B2 (en) 2003-07-18 2008-03-11 The Board Of Trustees Of The Leland Stanford Junior University Methods of decoupling reaction scale and protein synthesis yield in batch mode
AU2004276790A1 (en) 2003-09-23 2005-04-07 University Of Missouri Methods of synthesizing polynucleotides using thermostable enzymes
US7790431B2 (en) 2003-09-24 2010-09-07 Board Of Trustees Operating Michigan State University Methods and materials for the production of shikimic acid
DK1685240T3 (da) 2003-11-20 2015-02-16 Univ Leland Stanford Junior Forbedrede fremgangsmåder til in vitro-proteinsyntese
WO2005059157A2 (en) 2003-12-11 2005-06-30 The Board Of Trustees Of The Leland Stanford Junior University METHODS AND COMPOSITIONS FOR USE IN PREPARING HAIRPIN RNAs
US8298759B2 (en) 2004-03-25 2012-10-30 The Board Of Trustee Of The Leland Stanford Junior University Protein expression yield enhancement in cell-free protein synthesis systems by addition of antifoam agents
CA2564678C (en) 2004-04-27 2014-09-02 Archer-Daniels-Midland Company Enzymatic decarboxylation of 2-keto-l-gulonic acid to produce xylose
US8257943B2 (en) 2004-06-25 2012-09-04 Kyowa Hakko Bio Co., Ltd. Process for producing dipeptides or dipeptide derivatives
MX2007006780A (es) 2004-12-10 2007-08-06 Dsm Ip Assets Bv Produccion de beta-lactamas en celulas individuales.
WO2006090385A2 (en) * 2005-02-22 2006-08-31 Ramot At Tel-Aviv University Ltd. Protease inhibitors and method of screening thereof
US7351563B2 (en) 2005-06-10 2008-04-01 The Board Of Trustees Of The Leland Stanford Junior University Cell-free extracts and synthesis of active hydrogenase
US7312049B2 (en) 2005-06-14 2007-12-25 The Board Of Trustees Of The Leland Stanford Junior University Total amino acid stabilization during cell-free protein synthesis
TW200741005A (en) 2005-08-10 2007-11-01 Kyowa Hakko Kogyo Kk A purification method of cytidine diphosphate
CN1329506C (zh) 2005-09-06 2007-08-01 清华大学 一种具有颗粒状甲烷单加氧酶活性的重组菌及其应用
WO2007030772A2 (en) 2005-09-09 2007-03-15 The Johns Hopkins University Improved production of clavulanic acid by genetic engineering of streptomyces clavuligerus
US8183010B2 (en) 2005-10-31 2012-05-22 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of membrane bound polypeptides
US7579005B2 (en) * 2005-11-28 2009-08-25 E. I. Du Pont De Nemours And Company Process for recombinant expression and purification of antimicrobial peptides using periplasmic targeting signals as precipitable hydrophobic tags
GB0606112D0 (en) 2006-03-28 2006-05-03 Product and process
WO2007137144A2 (en) 2006-05-17 2007-11-29 University Of Medicine And Dentistry Of New Jersey Single protein production in living cells facilitated by a messenger rna interferase
WO2007140816A1 (en) 2006-06-09 2007-12-13 Metabolic Explorer Glycolic acid production by fermentation from renewable resources
WO2007144018A1 (en) 2006-06-12 2007-12-21 Metabolic Explorer Ethanolamine production by fermentation
US20110129438A1 (en) 2006-06-28 2011-06-02 James Robert Swartz Immunogenic protein constructs
US20100063258A1 (en) 2006-06-28 2010-03-11 The Board Of Trustees Of The Leland Stanford Junior University Fusion protein constructs
CA2657811C (en) 2006-06-29 2014-10-21 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of proteins containing unnatural amino acids
WO2008002673A2 (en) 2006-06-29 2008-01-03 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of virus like particles
WO2008062279A2 (en) 2006-11-20 2008-05-29 Orchid Chemicals & Pharmaceuticals Limited An improved process for the preparation of carbapenem antibiotic
KR100832740B1 (ko) 2007-01-17 2008-05-27 한국과학기술원 분지쇄 아미노산 생성능이 개선된 변이 미생물 및 이를이용한 분지쇄 아미노산의 제조방법
WO2008088884A2 (en) 2007-01-18 2008-07-24 The Board Of Trustees Of The Leland Stanford Junior University Enhanced cell-free synthesis of active proteins containing disulfide bonds
WO2008094546A2 (en) 2007-01-31 2008-08-07 The Regents Of The University Of California Genetically modified host cells for increased p450 activity levels and methods of use thereof
US20090124012A1 (en) 2007-08-08 2009-05-14 Mazef Biosciences, Llc Toxin/antitoxin systems and methods for regulating cellular growth, metabolic engineering and production of recombinant proteins
WO2009102205A1 (en) 2008-02-14 2009-08-20 Wageningen Universiteit Nucleotide sequences coding for cis-aconitic decarboxylase and use thereof
DK2262901T3 (da) 2008-03-05 2019-01-21 Genomatica Inc Organismer der producerer primær alkohol
JP2011519561A (ja) 2008-05-01 2011-07-14 ジェノマティカ， インコーポレイテッド メタクリル酸の産生のための微生物
RU2521502C2 (ru) 2008-07-28 2014-06-27 Б.Р.Э.И.Н. БАЙОТЕКНОЛОДЖИ РИСЕРЧ ЭНД ИНФОРМЕЙШН НЕТВОРК АГ,Германия Микробиологический способ получения 1,2-пропандиола
GB0819563D0 (en) 2008-10-24 2008-12-03 Isis Innovation Methods for preparing heterocyclic rings
BRPI0914521A2 (pt) 2008-10-27 2016-07-26 Butamax Advanced Biofuels Llc célula hospedeira microbiana recombinante, método de aumento da produção de isobutanol e método de produção de isobutanol
US20100143997A1 (en) 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions
EP2376619A4 (en) 2008-12-15 2012-07-04 Greenlight Biosciences Inc METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS
EP2379728A4 (en) 2008-12-22 2016-04-13 Greenlight Biosciences Inc COMPOSITIONS AND METHOD FOR PRODUCING A COMPOUND
EP2204453B1 (en) 2008-12-30 2013-03-13 Süd-Chemie IP GmbH & Co. KG Process for cell-free production of chemicals
US8597923B2 (en) 2009-05-06 2013-12-03 SyntheZyme, LLC Oxidation of compounds using genetically modified Candida
US10385367B2 (en) 2009-06-01 2019-08-20 Ginkgo Bioworks, Inc. Methods and molecules for yield improvement involving metabolic engineering
WO2011017560A1 (en) 2009-08-05 2011-02-10 Genomatica, Inc. Semi-synthetic terephthalic acid via microorganisms that produce muconic acid
EP2513112A4 (en) 2009-12-11 2013-05-08 Univ Johns Hopkins METHOD FOR THE LATE INTRODUCTION OF THE (8R) -HYDROXYL GROUP IN THE SYNTHESIS OF CARBAPENEMIC ANTIBIOTICS DERIVED FROM BETA-LACTAMES
US9040253B2 (en) 2010-04-14 2015-05-26 Sutro Biopharma, Inc. Method for enhancing recombinant protein production by cell-free protein expression system
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
JP2013526277A (ja) 2010-05-07 2013-06-24 グリーンライト バイオサイエンシーズ インコーポレーテッド 酵素リロケーションにより代謝経路のフラックスを制御する方法
EP2611922A1 (en) 2010-08-31 2013-07-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
JP5800218B2 (ja) 2011-07-20 2015-10-28 国立大学法人広島大学 Ａｔｐの製造方法およびその利用
CN104093848A (zh) 2011-09-09 2014-10-08 绿光生物科学公司 碳青霉烯(carbapenem)的无细胞制备
CA2896079A1 (en) 2012-12-21 2014-06-26 Greenlight Biosciences, Inc. Cell-free system for converting methane into fuel and chemical compounds
AU2014233711B2 (en) 2013-03-15 2020-05-28 Monsanto Technology Llc Compositions and Methods for the Production and Delivery of RNA
JP2016520324A (ja) 2013-06-05 2016-07-14 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． 無細胞生合成系における代謝フラックスの制御
CN104471771B (zh) 2013-06-05 2018-12-07 中国科学院天津工业生物技术研究所 使用无细胞合成酶促通路将糖完全氧化成电能
EP3460069B1 (en) 2013-08-05 2021-10-20 Greenlight Biosciences, Inc. Therapeutically active compounds and their methods of use
EP3277808A4 (en) 2015-03-30 2019-05-22 Greenlight Biosciences, Inc. ACELLULAR PRODUCTION OF RIBONUCLEIC ACID

Also Published As

Publication number Publication date
ES2839424T3 (es) 2021-07-05
EP3219796A1 (en) 2017-09-20
US10036001B2 (en) 2018-07-31
IL224637A (en) 2017-11-30
US8916358B2 (en) 2014-12-23
KR101988063B1 (ko) 2019-09-30
JP2013537802A (ja) 2013-10-07
JP6407228B2 (ja) 2018-10-17
JP2017029165A (ja) 2017-02-09
CA2809284A1 (en) 2012-03-08
EP2611922A1 (en) 2013-07-10
EP3219796B1 (en) 2020-10-07
US20150064751A1 (en) 2015-03-05
JP6280367B2 (ja) 2018-02-14
KR20130122729A (ko) 2013-11-08
WO2012030980A1 (en) 2012-03-08
CA2809284C (en) 2021-02-23
US20120052547A1 (en) 2012-03-01

Similar Documents

Publication Publication Date Title
DK3219796T3 (da) Fremgangsmåder til kontrol af strømningen i metaboliske reaktionsveje ved hjælp af proteasemanipulation
ZA201402826B (en) Vanadium flow cell
DE112011100381T8 (de) Flüssigkeitspatrone
DK3829174T3 (da) Fremgangsmåde til at konstruere fletningsliste
DK2692026T3 (da) Forspændingselement til fastholdelse af kontinuitet og tilknyttet fremgangsmåde
DK2609800T3 (da) Væskekølesystem til en server
DK2718422T3 (da) Dyrkningsmedier til stamceller
DK2718404T3 (da) Fremgangsmåde til fremstilling af flydende carbonhydrider
EP2705560A4 (en) PARTIAL FLOW CELL
DK2758172T3 (da) System til udførelse af reaktioner
BR112013014954A2 (pt) conjunto de fluxo de entrada
CO6960511A2 (es) Canilla mezcladora con filtro
DK2768442T3 (da) Fastgørelsesmekanisme til stomiposer
DK2776087T3 (da) Kit til transanal irrigation
FI20115703A0 (fi) Näytteenotin
BR112013021721A2 (pt) válvula misturadora de oxigênio
FR2955784B1 (fr) Extraction liquide / liquide
DK2654964T3 (da) Mikrofluidindretning til manipulering af partikler
DK3832291T3 (da) Gennemstrømningsmålecelle til optagelse af målemidler
GB201118288D0 (en) Ion-conducting membrane
DE112012003429A5 (de) Mischereinrichtung
DK2522421T3 (da) Fremgangsmåde til opnåelse af mikrokapsler
FR2974579B1 (fr) Composition nettoyante liquide
FR2958654B1 (fr) Liquide caloporteur
ZA201401031B (en) Liquid chemical composition