DK3186376T3 - Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet - Google Patents
Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet Download PDFInfo
- Publication number
- DK3186376T3 DK3186376T3 DK15763698.6T DK15763698T DK3186376T3 DK 3186376 T3 DK3186376 T3 DK 3186376T3 DK 15763698 T DK15763698 T DK 15763698T DK 3186376 T3 DK3186376 T3 DK 3186376T3
- Authority
- DK
- Denmark
- Prior art keywords
- methods
- manipulation efficiency
- cas9
- improving
- mediated manipulation
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/30—Endoribonucleases active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters (3.1.30)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462042358P | 2014-08-27 | 2014-08-27 | |
US201462047495P | 2014-09-08 | 2014-09-08 | |
PCT/US2015/047046 WO2016033246A1 (en) | 2014-08-27 | 2015-08-26 | Methods for increasing cas9-mediated engineering efficiency |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3186376T3 true DK3186376T3 (da) | 2019-05-06 |
Family
ID=54140655
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK15763698.6T DK3186376T3 (da) | 2014-08-27 | 2015-08-26 | Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet |
Country Status (11)
Country | Link |
---|---|
US (2) | US9970030B2 (da) |
EP (1) | EP3186376B1 (da) |
AU (1) | AU2015308910B2 (da) |
CA (1) | CA2959070C (da) |
DK (1) | DK3186376T3 (da) |
ES (1) | ES2730378T3 (da) |
GB (1) | GB2544001A (da) |
IL (1) | IL250700B (da) |
NZ (1) | NZ728437A (da) |
SG (1) | SG11201701245QA (da) |
WO (1) | WO2016033246A1 (da) |
Families Citing this family (54)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
BR112015026197B1 (pt) | 2013-04-16 | 2022-12-06 | Regeneron Pharmaceuticals, Inc | Método para modificação marcada de um lócus genômico de interesse em uma célula de rato pluripotente |
US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
RU2685914C1 (ru) | 2013-12-11 | 2019-04-23 | Регенерон Фармасьютикалс, Инк. | Способы и композиции для направленной модификации генома |
US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
LT3221457T (lt) | 2014-11-21 | 2019-06-10 | Regeneron Pharmaceuticals, Inc. | Nukreipiančios genetinės modifikacijos būdai ir kompozicijos, naudojant suporuotas kreipiančiąsias rnr sekas |
KR20240013283A (ko) | 2014-12-03 | 2024-01-30 | 애질런트 테크놀로지스, 인크. | 화학적 변형을 갖는 가이드 rna |
JP6873911B2 (ja) | 2015-04-06 | 2021-05-19 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | 初代細胞において標的核酸の遺伝子調節を誘導するためにインビトロで行う方法 |
EP3365357B1 (en) | 2015-10-23 | 2024-02-14 | President and Fellows of Harvard College | Evolved cas9 proteins for gene editing |
CN106893739A (zh) * | 2015-11-17 | 2017-06-27 | 香港中文大学 | 用于靶向基因操作的新方法和*** |
WO2017147056A1 (en) * | 2016-02-22 | 2017-08-31 | Caribou Biosciences, Inc. | Methods for modulating dna repair outcomes |
WO2017155408A1 (en) * | 2016-03-11 | 2017-09-14 | Erasmus University Medical Center Rotterdam | Improved crispr-cas9 genome editing tool |
EP3219799A1 (en) | 2016-03-17 | 2017-09-20 | IMBA-Institut für Molekulare Biotechnologie GmbH | Conditional crispr sgrna expression |
WO2017180711A1 (en) * | 2016-04-13 | 2017-10-19 | Editas Medicine, Inc. | Grna fusion molecules, gene editing systems, and methods of use thereof |
CA3022319A1 (en) * | 2016-05-06 | 2017-11-09 | Tod M. Woolf | Improved methods for genome editing with and without programmable nucleases |
EP3907286A1 (en) * | 2016-06-02 | 2021-11-10 | Sigma-Aldrich Co., LLC | Using programmable dna binding proteins to enhance targeted genome modification |
US10767175B2 (en) | 2016-06-08 | 2020-09-08 | Agilent Technologies, Inc. | High specificity genome editing using chemically modified guide RNAs |
US20200340011A1 (en) * | 2016-06-10 | 2020-10-29 | University Of Wyoming | Recombinant insect vectors and methods of use |
JP6855037B2 (ja) * | 2016-07-19 | 2021-04-07 | 国立大学法人大阪大学 | ゲノム編集方法 |
AU2017306676B2 (en) | 2016-08-03 | 2024-02-22 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
EP3500090A4 (en) | 2016-08-18 | 2020-01-08 | Canopy Growth Corporation | PLANTS AND METHODS FOR INCREASING AND DECREASING CANNABINOID SYNTHESIS |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
US11332727B2 (en) | 2017-03-14 | 2022-05-17 | The Regents Of The University Of California | Method for reducing an immune response by administering an immune evading adeno-associated AAV8 or AAVDJ viral vector |
IL306092A (en) | 2017-03-23 | 2023-11-01 | Harvard College | Nucleic base editors that include nucleic acid programmable DNA binding proteins |
WO2018179578A1 (ja) * | 2017-03-30 | 2018-10-04 | 国立大学法人京都大学 | ゲノム編集によるエクソンスキッピング誘導方法 |
KR20230084312A (ko) * | 2017-04-10 | 2023-06-12 | 막스-플랑크-게젤샤프트 츄어 푀르더룽 데어 비쎈샤프텐 에.파우. | 유전체 편집 효율 증가를 위한 화합물 |
WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化(pace)を用いて塩基編集因子を進化させるための方法および組成物 |
WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
JP7423520B2 (ja) * | 2017-11-16 | 2024-01-29 | アストラゼネカ・アクチエボラーグ | Cas9ベースノックイン方針の効力を改善するための組成物及び方法 |
WO2019099982A1 (en) * | 2017-11-17 | 2019-05-23 | The Johns Hopkins University | Compositions and methods for efficient genome editing |
GB2589246A (en) | 2018-05-16 | 2021-05-26 | Synthego Corp | Methods and systems for guide RNA design and use |
WO2020081438A1 (en) | 2018-10-16 | 2020-04-23 | Blueallele, Llc | Methods for targeted insertion of dna in genes |
JP2022526908A (ja) | 2019-03-19 | 2022-05-27 | ザ ブロード インスティテュート,インコーポレーテッド | 編集ヌクレオチド配列を編集するための方法および組成物 |
CA3140132A1 (en) * | 2019-05-29 | 2020-12-03 | Monsanto Technology Llc | Directed genome engineering using enhanced targeted editing technologies |
AU2021267940A1 (en) | 2020-05-08 | 2022-12-08 | President And Fellows Of Harvard College | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
GB202007943D0 (en) * | 2020-05-27 | 2020-07-08 | Snipr Biome Aps | Products & methods |
US20230416725A1 (en) * | 2020-09-15 | 2023-12-28 | Rutgers, The State University Of New Jersey | Systems for gene editing and methods of use thereof |
WO2022256448A2 (en) * | 2021-06-01 | 2022-12-08 | Artisan Development Labs, Inc. | Compositions and methods for targeting, editing, or modifying genes |
AU2022343300A1 (en) | 2021-09-10 | 2024-04-18 | Agilent Technologies, Inc. | Guide rnas with chemical modification for prime editing |
CN113801852B (zh) * | 2021-10-18 | 2023-08-18 | 齐齐哈尔医学院 | 一种gpd1l缺失人胚胎干细胞细胞株及其构建方法和应用 |
WO2023148291A1 (en) * | 2022-02-02 | 2023-08-10 | Biotalys NV | Methods for genome editing |
CN114703174B (zh) * | 2022-04-12 | 2023-10-24 | 中国科学院海洋研究所 | 快速获得基因型和表型突变的CRISPR/Cas9基因敲除方法及应用 |
Family Cites Families (55)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1152058A1 (en) * | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
US10087431B2 (en) | 2010-03-10 | 2018-10-02 | The Regents Of The University Of California | Methods of generating nucleic acid fragments |
SG185481A1 (en) | 2010-05-10 | 2012-12-28 | Univ California | Endoribonuclease compositions and methods of use thereof |
WO2013049330A1 (en) | 2011-09-28 | 2013-04-04 | The Regents Of The University Of California | Synthetic transcriptional control elements and methods of generating and using such elements |
US9895451B2 (en) | 2011-12-02 | 2018-02-20 | Yale University | Formulations for targeted release of agents to low pH tissue environments or cellular compartments and methods of use thereof |
GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
WO2013122816A1 (en) | 2012-02-13 | 2013-08-22 | The Regents Of The University Of California | Methods for genome-wide screening and construction of genetic interaction maps |
JP6490426B2 (ja) | 2012-02-29 | 2019-03-27 | サンガモ セラピューティクス, インコーポレイテッド | ハンチントン病を治療するための方法および組成物 |
AU2013235340B2 (en) | 2012-03-17 | 2019-05-23 | The Regents Of The University Of California | Fast diagnosis and personalized treatments for acne |
WO2013141680A1 (en) | 2012-03-20 | 2013-09-26 | Vilnius University | RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX |
US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
LT3401400T (lt) | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
US9688971B2 (en) | 2012-06-15 | 2017-06-27 | The Regents Of The University Of California | Endoribonuclease and methods of use thereof |
PT3494997T (pt) | 2012-07-25 | 2019-12-05 | Massachusetts Inst Technology | Proteínas de ligação a adn indutíveis e ferramentas de perturbação do genoma e aplicações destas |
WO2014022702A2 (en) | 2012-08-03 | 2014-02-06 | The Regents Of The University Of California | Methods and compositions for controlling gene expression by rna processing |
ES2812599T3 (es) | 2012-08-29 | 2021-03-17 | Sangamo Therapeutics Inc | Procedimientos y composiciones para el tratamiento de una afección genética |
UA118090C2 (uk) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив |
DE202013012597U1 (de) * | 2012-10-23 | 2017-11-21 | Toolgen, Inc. | Zusammensetzung zum Spalten einer Ziel-DNA, umfassend eine für die Ziel-DNA spezifische guide-RNA und eine Cas-Protein-codierende Nukleinsäure oder ein Cas-Protein, sowie deren Verwendung |
US20150291967A1 (en) | 2012-10-31 | 2015-10-15 | Luc Mathis | Coupling herbicide resistance with targeted insertion of transgenes in plants |
WO2014089541A2 (en) | 2012-12-07 | 2014-06-12 | Haplomics, Inc. | Factor viii mutation repair and tolerance induction |
EP4234696A3 (en) | 2012-12-12 | 2023-09-06 | The Broad Institute Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
ES2658401T3 (es) | 2012-12-12 | 2018-03-09 | The Broad Institute, Inc. | Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas |
US8697359B1 (en) | 2012-12-12 | 2014-04-15 | The Broad Institute, Inc. | CRISPR-Cas systems and methods for altering expression of gene products |
DK2898075T3 (da) | 2012-12-12 | 2016-06-27 | Broad Inst Inc | Konstruktion og optimering af forbedrede systemer, fremgangsmåder og enzymsammensætninger til sekvensmanipulation |
PL2784162T3 (pl) | 2012-12-12 | 2016-01-29 | Broad Inst Inc | Opracowanie systemów, metod oraz zoptymalizowanych kompozycji przewodnikowych do manipulacji sekwencyjnej |
ES2576128T3 (es) | 2012-12-12 | 2016-07-05 | The Broad Institute, Inc. | Modificación por tecnología genética y optimización de sistemas, métodos y composiciones para la manipulación de secuencias con dominios funcionales |
PT2921557T (pt) | 2012-12-12 | 2016-10-19 | Massachusetts Inst Technology | Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências |
CA3081054A1 (en) * | 2012-12-17 | 2014-06-26 | President And Fellows Of Harvard College | Rna-guided human genome engineering |
CN105120656A (zh) | 2012-12-21 | 2015-12-02 | 塞尔克蒂斯股份有限公司 | 冷诱导甜味降低的马铃薯 |
CN103981147B (zh) | 2013-02-08 | 2017-11-10 | 中国科学院上海生命科学研究院 | 一种新的制备肝实质细胞的方法 |
US20140288411A1 (en) | 2013-03-12 | 2014-09-25 | The Regents Of The University Of California | Gas vesicle magnetic resonance imaging contrast agents and methods of using the same |
US20140288421A1 (en) | 2013-03-12 | 2014-09-25 | The Regents Of The University Of California | Gas vesicle ultrasound contrast agents and methods of using the same |
KR101780885B1 (ko) | 2013-03-14 | 2017-10-11 | 카리부 바이오사이언시스 인코포레이티드 | 핵산-표적화 핵산의 조성물 및 방법 |
US11332719B2 (en) | 2013-03-15 | 2022-05-17 | The Broad Institute, Inc. | Recombinant virus and preparations thereof |
US9937207B2 (en) | 2013-03-21 | 2018-04-10 | Sangamo Therapeutics, Inc. | Targeted disruption of T cell receptor genes using talens |
EP4286517A3 (en) | 2013-04-04 | 2024-03-13 | President and Fellows of Harvard College | Therapeutic uses of genome editing with crispr/cas systems |
US9902973B2 (en) | 2013-04-11 | 2018-02-27 | Caribou Biosciences, Inc. | Methods of modifying a target nucleic acid with an argonaute |
CA2910489A1 (en) | 2013-05-15 | 2014-11-20 | Sangamo Biosciences, Inc. | Methods and compositions for treatment of a genetic condition |
EP2999788B1 (en) | 2013-05-22 | 2020-07-08 | Northwestern University | Rna-directed dna cleavage and gene editing by cas9 enzyme from neisseria meningitidis |
EP3004349B1 (en) | 2013-05-29 | 2018-03-28 | Cellectis S.A. | A method for producing precise dna cleavage using cas9 nickase activity |
AU2014274939B2 (en) | 2013-06-04 | 2020-03-19 | President And Fellows Of Harvard College | RNA-guideded transcriptional regulation |
US20140356956A1 (en) | 2013-06-04 | 2014-12-04 | President And Fellows Of Harvard College | RNA-Guided Transcriptional Regulation |
CA2920899C (en) | 2013-08-28 | 2023-02-28 | Sangamo Biosciences, Inc. | Compositions for linking dna-binding domains and cleavage domains |
EP3071695A2 (en) | 2013-11-18 | 2016-09-28 | Crispr Therapeutics AG | Crispr-cas system materials and methods |
EP3757116A1 (en) | 2013-12-09 | 2020-12-30 | Sangamo Therapeutics, Inc. | Methods and compositions for genome engineering |
MX2016007327A (es) | 2013-12-12 | 2017-03-06 | Broad Inst Inc | Suministro, uso y aplicaciones terapeuticas de sistemas y composiciones crispr-cas para dirigirlos a trastornos y enfermedades usando componentes para suministro de particulas. |
US20150191744A1 (en) | 2013-12-17 | 2015-07-09 | University Of Massachusetts | Cas9 effector-mediated regulation of transcription, differentiation and gene editing/labeling |
US10774338B2 (en) | 2014-01-16 | 2020-09-15 | The Regents Of The University Of California | Generation of heritable chimeric plant traits |
PL3102673T3 (pl) | 2014-02-03 | 2020-11-02 | Sangamo Therapeutics, Inc. | Sposoby i kompozycje do leczenia talasemii beta |
US9624498B2 (en) | 2014-03-18 | 2017-04-18 | Sangamo Biosciences, Inc. | Methods and compositions for regulation of zinc finger protein expression |
MX2016014565A (es) | 2014-05-08 | 2017-05-23 | Sangamo Biosciences Inc | Metodos y composiciones para tratar la enfermedad de huntington. |
US9944933B2 (en) * | 2014-07-17 | 2018-04-17 | Georgia Tech Research Corporation | Aptamer-guided gene targeting |
CA2963080A1 (en) * | 2014-10-01 | 2016-04-07 | The General Hospital Corporation | Methods for increasing efficiency of nuclease-induced homology-directed repair |
-
2015
- 2015-08-26 AU AU2015308910A patent/AU2015308910B2/en active Active
- 2015-08-26 CA CA2959070A patent/CA2959070C/en active Active
- 2015-08-26 US US14/836,753 patent/US9970030B2/en active Active
- 2015-08-26 GB GB1703026.3A patent/GB2544001A/en not_active Withdrawn
- 2015-08-26 ES ES15763698T patent/ES2730378T3/es active Active
- 2015-08-26 EP EP15763698.6A patent/EP3186376B1/en active Active
- 2015-08-26 DK DK15763698.6T patent/DK3186376T3/da active
- 2015-08-26 WO PCT/US2015/047046 patent/WO2016033246A1/en active Application Filing
- 2015-08-26 SG SG11201701245QA patent/SG11201701245QA/en unknown
- 2015-08-26 NZ NZ728437A patent/NZ728437A/en unknown
-
2017
- 2017-02-21 IL IL250700A patent/IL250700B/en active IP Right Grant
-
2018
- 2018-02-02 US US15/887,893 patent/US20180171360A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20160257973A1 (en) | 2016-09-08 |
IL250700B (en) | 2020-10-29 |
SG11201701245QA (en) | 2017-03-30 |
GB201703026D0 (en) | 2017-04-12 |
ES2730378T3 (es) | 2019-11-11 |
WO2016033246A1 (en) | 2016-03-03 |
US20180171360A1 (en) | 2018-06-21 |
CA2959070C (en) | 2020-11-10 |
NZ728437A (en) | 2018-02-23 |
IL250700A0 (en) | 2017-04-30 |
AU2015308910A1 (en) | 2017-02-09 |
CA2959070A1 (en) | 2016-03-03 |
GB2544001A (en) | 2017-05-03 |
EP3186376A1 (en) | 2017-07-05 |
US9970030B2 (en) | 2018-05-15 |
AU2015308910B2 (en) | 2017-12-07 |
EP3186376B1 (en) | 2019-03-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
FIC20230037I1 (fi) | Glofitamabi | |
DK3186376T3 (da) | Fremgangsmåder til forbedring af cas9-medieret manipulationseffektivitet | |
DK3110988T3 (da) | Metoder til behandling af lithiumholdige materialer | |
DK3134506T3 (da) | Fremgangsmåder til oprensning af messenger rna | |
DK3134402T3 (da) | 4-amino-imidazoquinolinforbindelser | |
DK3183051T3 (da) | Væske-til-luftmembranenergivekslere | |
DK3294700T3 (da) | Fremgangsmåde til fremstilling af limonen-4-ol | |
DK3211743T3 (da) | Ø-til-netværk-omskiftningsfremgangsmåde | |
DK3222714T3 (da) | Phytasemutanter | |
MA55696A (fr) | Combinaisons | |
DK3119396T3 (da) | Muscarinreceptoragonister | |
DK3006072T3 (da) | Karadgang | |
DK3125898T3 (da) | Farmakofor til trail-induktion | |
DE112015001664A5 (de) | Betätigungsaktuator | |
DK3183340T3 (da) | Termolabile exonukleaser | |
DK3154703T3 (da) | Fuldkappe-snekkecentrifuge | |
DK3110439T3 (da) | Pcsk9-vacciner | |
DE112015005041A5 (de) | Gargerätevorrichtung | |
DE112015002984A5 (de) | Positioniereinheit | |
DE102014000623A8 (de) | Halbhohlnietelement | |
DK3009858T3 (da) | Skyradar | |
DK3154942T3 (da) | Fremstilling af piperidin-4-carbothioamid | |
DK2942098T3 (da) | Forbedret fremgangsmåde til fjernelse af nitrit | |
DE112015001633A5 (de) | Betätigungsaktuator | |
DK3447493T3 (da) | Proteinrettede ortologer |