DK2077280T3 - Carbohydrate Deficient Transferrin (CDT)-specifikke antistoffer, fremstilling og anvendelse af disse - Google Patents

Carbohydrate Deficient Transferrin (CDT)-specifikke antistoffer, fremstilling og anvendelse af disse Download PDF

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Publication number
DK2077280T3
DK2077280T3 DK09005276.2T DK09005276T DK2077280T3 DK 2077280 T3 DK2077280 T3 DK 2077280T3 DK 09005276 T DK09005276 T DK 09005276T DK 2077280 T3 DK2077280 T3 DK 2077280T3
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DK
Denmark
Prior art keywords
cdt
antibody
transferrin
antibodies
cells
Prior art date
Application number
DK09005276.2T
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English (en)
Inventor
Harald Althaus
Original Assignee
Siemens Healthcare Diagnostics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Siemens Healthcare Diagnostics filed Critical Siemens Healthcare Diagnostics
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Publication of DK2077280T3 publication Critical patent/DK2077280T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/79—Transferrins, e.g. lactoferrins, ovotransferrins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Claims (6)

1. Monoklonalt antistof, fremstillet ved immunisering af et egnet, ikke-menne-skeligt forsøgsdyr med ikke-glykosyleret transferrin eller carbohydrate deficient transferrin (CDT) og etablering af hybridomceller, som i en vandig opløsning binder selektivt til CDT, uden at dette behøver at være bundet til en fast fase, og dettes binding foregår i området ved de følgende sekvensafsnit (1) til (4) af CDT'et: (1) VVARSMGGKEDLIWELL og (2) TTEDSIAKIMNGEADAMSLDGGF og (3) SKLSMGSGLNLSEPN og (4) YEKYLGEEYVKAV.
2. Monoklonalt antistof ifølge krav 1, kendetegnet ved, at det produceres af cellekulturen med deponeringsnummeret DSM ACC2540.
3. Antigenbindende fragment af et antistof ifølge et af de foregående krav 1 eller 2, hvilket har den samme antigenspecifitet som det antistof, der er defineret i et af disse krav.
4. Fremgangsmåde til fremstilling af antistoffet ifølge krav 1 ved immunisering af et egnet, ikke-menneskeligt forsøgsdyr med ikke-glykosyleret transferrin eller CDT, fusionering af miltcellerne fra dette forsøgsdyr med myelomcel-ler, hvorved der opstår antistofproducerende hybridceller, kloning af hybridcellerne og selektering af en sådan hybridcelleklon, som i en vandig opløsning binder selektivt til CDT, og dettes binding foregår i området ved de følgende sekvensafsnit (1) til (4) af CDT: (1) VVARSMGGKEDLIWELL og (2) TTEDSIAKIMNGEADAMSLDGGF og (3) SKLSMGSGLNLSEPN og (4) YEKYLGEEYVKAV.
5. Immunoassay til påvisning af CDT i en prøve, kendetegnet ved, at et antistof ifølge et af kravene 1 eller 2 eller det antigenbindende fragment ifølge krav 3 bringes i kontakt med prøven, og dannelsen af et immunkompleks under deltagelse af CDT bestemmes kvalitativt eller kvantitativt.
6. Testkit til udførelse af et immunoassay ifølge krav 5 indeholdende et antistof ifølge et af kravene 1 eller 2 eller det antigenbindende fragment ifølge krav 3.
DK09005276.2T 2002-07-05 2003-05-19 Carbohydrate Deficient Transferrin (CDT)-specifikke antistoffer, fremstilling og anvendelse af disse DK2077280T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2002130550 DE10230550A1 (de) 2002-07-05 2002-07-05 Carbohydrate Deficient Transferrin (CDT)-spezifische Antikörper, ihre Herstellung und Verwendung
EP03011334A EP1378521A1 (de) 2002-07-05 2003-05-19 Carbohydrate Deficient Transferrin (CDT)-spezifische Antikörper, ihre Herstellung und Verwendung

Publications (1)

Publication Number Publication Date
DK2077280T3 true DK2077280T3 (da) 2015-08-24

Family

ID=29719498

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09005276.2T DK2077280T3 (da) 2002-07-05 2003-05-19 Carbohydrate Deficient Transferrin (CDT)-specifikke antistoffer, fremstilling og anvendelse af disse

Country Status (4)

Country Link
EP (2) EP1378521A1 (da)
DE (1) DE10230550A1 (da)
DK (1) DK2077280T3 (da)
ES (1) ES2542699T3 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9663567B2 (en) * 2011-11-09 2017-05-30 Alper Biotech Llc Monoclonal antibodies against serotransferrin antigens, and uses therefor
WO2014144060A1 (en) * 2013-03-15 2014-09-18 Alper Biotech, Llc Monoclonal antibodies to transferrin and transferrin receptor antigens, and uses thereof
CN106461684B (zh) 2014-03-12 2018-07-27 维罗纳大学 用于识别至少一种转铁蛋白糖型的分析方法
CN116041506B (zh) * 2023-01-05 2023-12-05 深圳上泰生物工程有限公司 糖缺失性转铁蛋白的单克隆抗体对及检测试剂盒

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69229230T2 (de) * 1991-09-25 2000-01-27 Byron E. Anderson Antikörpertest zur erkennung von alkoholikern und zur überwachung der alkoholaufnahme
GB9713559D0 (en) * 1997-06-26 1997-09-03 Axis Biochemicals Asa Assay
GB9827411D0 (en) * 1998-12-11 1999-02-03 Axis Biochemicals Asa Dipstick assay

Also Published As

Publication number Publication date
ES2542699T3 (es) 2015-08-10
EP2077280B1 (de) 2015-06-24
EP2077280A3 (de) 2010-03-24
EP1378521A1 (de) 2004-01-07
DE10230550A1 (de) 2004-01-15
EP2077280A2 (de) 2009-07-08

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