DK1969127T3 - Fremgangsmåde til fremstilling af biologisk aktive vitamin K-afhængige proteiner ved rekombinante fremgangsmåder - Google Patents

Fremgangsmåde til fremstilling af biologisk aktive vitamin K-afhængige proteiner ved rekombinante fremgangsmåder

Info

Publication number
DK1969127T3
DK1969127T3 DK06848864.2T DK06848864T DK1969127T3 DK 1969127 T3 DK1969127 T3 DK 1969127T3 DK 06848864 T DK06848864 T DK 06848864T DK 1969127 T3 DK1969127 T3 DK 1969127T3
Authority
DK
Denmark
Prior art keywords
preparation
biologically active
recombinant methods
active vitamin
dependent proteins
Prior art date
Application number
DK06848864.2T
Other languages
English (en)
Other versions
DK1969127T4 (da
Inventor
William N Drohan
Michael J Griffith
John R Taylor
Darrel W Stafford
Original Assignee
Cnj Holdings Inc
Univ North Carolina
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38051872&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK1969127(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Cnj Holdings Inc, Univ North Carolina filed Critical Cnj Holdings Inc
Publication of DK1969127T3 publication Critical patent/DK1969127T3/da
Application granted granted Critical
Publication of DK1969127T4 publication Critical patent/DK1969127T4/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/04—Oxidoreductases acting on the CH-OH group of donors (1.1) with a disulfide as acceptor (1.1.4)
    • C12Y101/04001—Vitamin-K-epoxide reductase (warfarin-sensitive) (1.1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/0109—Peptidyl-glutamate 4-carboxylase (4.1.1.90)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Diabetes (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK06848864.2T 2005-12-21 2006-12-21 Fremgangsmåde til fremstilling af biologisk aktivt vitamin K-afhængige proteiner med rekombinante fremgangsmåder DK1969127T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US75264205P 2005-12-21 2005-12-21
PCT/US2006/048954 WO2007075976A2 (en) 2005-12-21 2006-12-21 Method of producing biologically active vitamin k dependent proteins by recombinant methods

Publications (2)

Publication Number Publication Date
DK1969127T3 true DK1969127T3 (da) 2014-09-22
DK1969127T4 DK1969127T4 (da) 2017-10-16

Family

ID=38051872

Family Applications (1)

Application Number Title Priority Date Filing Date
DK06848864.2T DK1969127T4 (da) 2005-12-21 2006-12-21 Fremgangsmåde til fremstilling af biologisk aktivt vitamin K-afhængige proteiner med rekombinante fremgangsmåder

Country Status (11)

Country Link
US (6) US20080045453A1 (da)
EP (2) EP1969127B8 (da)
JP (3) JP5526332B2 (da)
AU (1) AU2006331501B2 (da)
CA (1) CA2633661C (da)
DK (1) DK1969127T4 (da)
ES (1) ES2503365T5 (da)
PL (1) PL1969127T5 (da)
PT (1) PT1969127E (da)
SI (1) SI1969127T2 (da)
WO (1) WO2007075976A2 (da)

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EP1670947B1 (en) 2003-09-23 2015-04-01 University Of North Carolina At Chapel Hill Methods and compositions for the correlation of single nucleotide polymorphisms in the vitamin k epoxide reductase gene and warfarin dosage
JP4777251B2 (ja) 2003-10-14 2011-09-21 バクスター・インターナショナル・インコーポレイテッド ビタミンｋエポキシド還元酵素複合体サブユニット１ｖｋｏｒｃ１、クマリンおよびその誘導体の治療標的
GB0324044D0 (en) * 2003-10-14 2003-11-19 Astrazeneca Ab Protein
EP1861499A4 (en) 2005-03-15 2008-09-03 Univ North Carolina METHODS AND COMPOSITIONS FOR THE PRODUCTION OF ACTIVE PROTEINS DEPENDENT ON VITAMIN K
KR20130140221A (ko) 2005-04-13 2013-12-23 아스트라제네카 아베 감마 카르복실화를 필요로 하는 단백질 생산용 벡터를 포함하는 숙주 세포
EP1969127B8 (en) † 2005-12-21 2017-12-13 Aptevo BioTherapeutics LLC Method of producing biologically active vitamin k dependent proteins by recombinant methods
EP2139499A4 (en) * 2007-04-26 2010-05-05 Inspiration Biopharmaceuticals RECOMBINANT VITAMIN K-DEPENDENT PROTEINS WITH HIGH SIALIC ACID CONTENT AND METHOD FOR THE PRODUCTION THEREOF
US8206967B2 (en) * 2007-07-06 2012-06-26 Medimmune Limited Method for production of recombinant human thrombin
DK3581650T3 (da) 2008-09-15 2023-03-13 Uniqure Biopharma B V Faktor ix polypeptidmutant, anvendelse deraf og en fremgangsmåde til fremstilling deraf
AU2010269072C9 (en) * 2009-07-10 2014-09-18 Csl Limited Method of increasing the expression yield of vitamin K-dependent proteins
MX2012003816A (es) * 2009-10-01 2012-07-04 Toto Ltd Construccion de adn y procedimiento para la generacion de celulas cho recombinantes usando la misma.
WO2011053738A1 (en) * 2009-10-30 2011-05-05 Inspiration Biopharmaceuticals, Inc. Method of producing recombinant vitamin k dependent proteins
WO2012088222A2 (en) 2010-12-21 2012-06-28 The University Of North Carolina At Chapel Hill Methods and compositions for producing active vitamin k-dependent proteins
JP2015507929A (ja) * 2012-02-14 2015-03-16 ポートラ ファーマシューティカルズ， インコーポレイテッド 第Ｘａ因子阻害剤に対する組み換え拮抗剤を製作するためのプロセス
CA2904125C (en) * 2013-03-12 2022-06-07 Cmc Icos Biologics, Inc. Improved recombinant protein expression using a hybrid chef1 promoter
JP5830486B2 (ja) * 2013-03-29 2015-12-09 シスメックス株式会社 組換えバキュロウイルスおよびその利用
RU2585532C2 (ru) * 2014-01-31 2016-05-27 Федеральное государственное учреждение "Федеральный исследовательский центр "Фундаментальные основы биотехнологии" Российской академии наук"(ФИЦ Биотехнологии РАН) Плазмида для экспрессии рекомбинантного фактора свёртываемости крови ix человека, клетка сно - продуцент рекомбинантного фактора свёртываемости крови ix человека и способ получения указанного фактора
AU2016363789B2 (en) * 2015-12-02 2022-06-02 CSL Behring Lengnau AG Improved media for the expression of recombinant vitamin K-dependent proteins

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EP1670947B1 (en) * 2003-09-23 2015-04-01 University Of North Carolina At Chapel Hill Methods and compositions for the correlation of single nucleotide polymorphisms in the vitamin k epoxide reductase gene and warfarin dosage
GB0324044D0 (en) * 2003-10-14 2003-11-19 Astrazeneca Ab Protein
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EP1861499A4 (en) * 2005-03-15 2008-09-03 Univ North Carolina METHODS AND COMPOSITIONS FOR THE PRODUCTION OF ACTIVE PROTEINS DEPENDENT ON VITAMIN K
KR20130140221A (ko) * 2005-04-13 2013-12-23 아스트라제네카 아베 감마 카르복실화를 필요로 하는 단백질 생산용 벡터를 포함하는 숙주 세포
EP1969127B8 (en) † 2005-12-21 2017-12-13 Aptevo BioTherapeutics LLC Method of producing biologically active vitamin k dependent proteins by recombinant methods

Also Published As

Publication number Publication date
US20230287471A1 (en) 2023-09-14
WO2007075976A2 (en) 2007-07-05
JP2013223514A (ja) 2013-10-31
SI1969127T1 (sl) 2014-12-31
JP2017046717A (ja) 2017-03-09
JP5526332B2 (ja) 2014-06-18
PL1969127T3 (pl) 2014-11-28
EP2385125A2 (en) 2011-11-09
DK1969127T4 (da) 2017-10-16
EP1969127B8 (en) 2017-12-13
WO2007075976A3 (en) 2007-10-04
ES2503365T5 (es) 2017-10-31
US20130266982A1 (en) 2013-10-10
PL1969127T5 (pl) 2018-02-28
SI1969127T2 (sl) 2017-10-30
CA2633661A1 (en) 2007-07-05
US20210095323A1 (en) 2021-04-01
CA2633661C (en) 2019-06-04
EP2385125A3 (en) 2012-04-11
ES2503365T3 (es) 2014-10-06
US20160108449A1 (en) 2016-04-21
JP2009521224A (ja) 2009-06-04
EP1969127B1 (en) 2014-06-18
EP1969127A2 (en) 2008-09-17
US20190032101A1 (en) 2019-01-31
EP1969127B2 (en) 2017-07-12
US20080045453A1 (en) 2008-02-21
AU2006331501A1 (en) 2007-07-05
PT1969127E (pt) 2014-09-23
AU2006331501B2 (en) 2013-09-05

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