DK1929012T3 - Fremgangsmåde til in vitro-rekombination - Google Patents
Fremgangsmåde til in vitro-rekombinationInfo
- Publication number
- DK1929012T3 DK1929012T3 DK06836102.1T DK06836102T DK1929012T3 DK 1929012 T3 DK1929012 T3 DK 1929012T3 DK 06836102 T DK06836102 T DK 06836102T DK 1929012 T3 DK1929012 T3 DK 1929012T3
- Authority
- DK
- Denmark
- Prior art keywords
- molecules
- dna
- region
- stranded
- substantially purified
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Battery Electrode And Active Subsutance (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US70717705P | 2005-08-11 | 2005-08-11 | |
US80040006P | 2006-05-16 | 2006-05-16 | |
PCT/US2006/031214 WO2007032837A2 (en) | 2005-08-11 | 2006-08-11 | Method for in vitro recombination |
Publications (1)
Publication Number | Publication Date |
---|---|
DK1929012T3 true DK1929012T3 (da) | 2011-01-31 |
Family
ID=37734039
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK10168787.9T DK2239327T3 (da) | 2005-08-11 | 2006-08-11 | Fremgangsmåde til in vitro rekombination |
DK06836102.1T DK1929012T3 (da) | 2005-08-11 | 2006-08-11 | Fremgangsmåde til in vitro-rekombination |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK10168787.9T DK2239327T3 (da) | 2005-08-11 | 2006-08-11 | Fremgangsmåde til in vitro rekombination |
Country Status (8)
Country | Link |
---|---|
US (3) | US7776532B2 (da) |
EP (2) | EP2239327B1 (da) |
AT (1) | ATE483802T1 (da) |
CA (1) | CA2618665C (da) |
DE (1) | DE602006017405D1 (da) |
DK (2) | DK2239327T3 (da) |
MY (1) | MY144014A (da) |
WO (1) | WO2007032837A2 (da) |
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CN102124112B (zh) | 2008-09-10 | 2013-03-20 | 金斯瑞公司 | 基于同源重组dna的克隆方法及组合物 |
US9234226B2 (en) * | 2008-10-14 | 2016-01-12 | Wayne M. Barnes | In vitro recombination methods using 5′ exonuclease |
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CN113604546A (zh) | 2015-09-22 | 2021-11-05 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
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-
2006
- 2006-08-11 CA CA2618665A patent/CA2618665C/en active Active
- 2006-08-11 WO PCT/US2006/031214 patent/WO2007032837A2/en active Application Filing
- 2006-08-11 EP EP10168787.9A patent/EP2239327B1/en active Active
- 2006-08-11 EP EP06836102A patent/EP1929012B1/en active Active
- 2006-08-11 DE DE602006017405T patent/DE602006017405D1/de active Active
- 2006-08-11 MY MYPI20063911A patent/MY144014A/en unknown
- 2006-08-11 DK DK10168787.9T patent/DK2239327T3/da active
- 2006-08-11 US US11/502,624 patent/US7776532B2/en active Active
- 2006-08-11 AT AT06836102T patent/ATE483802T1/de not_active IP Right Cessation
- 2006-08-11 DK DK06836102.1T patent/DK1929012T3/da active
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2010
- 2010-06-22 US US12/820,861 patent/US8435736B2/en active Active
-
2013
- 2013-04-16 US US13/864,108 patent/US20130295645A1/en not_active Abandoned
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Publication number | Publication date |
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US8435736B2 (en) | 2013-05-07 |
EP1929012B1 (en) | 2010-10-06 |
EP2239327B1 (en) | 2015-02-25 |
CA2618665C (en) | 2012-11-13 |
EP2239327A2 (en) | 2010-10-13 |
CA2618665A1 (en) | 2007-03-22 |
DE602006017405D1 (de) | 2010-11-18 |
US20100311126A1 (en) | 2010-12-09 |
WO2007032837A3 (en) | 2007-05-24 |
US7776532B2 (en) | 2010-08-17 |
EP2239327A3 (en) | 2012-04-25 |
MY144014A (en) | 2011-07-29 |
WO2007032837A2 (en) | 2007-03-22 |
DK2239327T3 (da) | 2015-05-18 |
EP1929012A2 (en) | 2008-06-11 |
ATE483802T1 (de) | 2010-10-15 |
US20070037196A1 (en) | 2007-02-15 |
US20130295645A1 (en) | 2013-11-07 |
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