DK1929012T3 - Fremgangsmåde til in vitro-rekombination - Google Patents
Fremgangsmåde til in vitro-rekombinationInfo
- Publication number
- DK1929012T3 DK1929012T3 DK06836102.1T DK06836102T DK1929012T3 DK 1929012 T3 DK1929012 T3 DK 1929012T3 DK 06836102 T DK06836102 T DK 06836102T DK 1929012 T3 DK1929012 T3 DK 1929012T3
- Authority
- DK
- Denmark
- Prior art keywords
- molecules
- dna
- region
- stranded
- substantially purified
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Battery Electrode And Active Subsutance (AREA)
- Crystals, And After-Treatments Of Crystals (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US70717705P | 2005-08-11 | 2005-08-11 | |
| US80040006P | 2006-05-16 | 2006-05-16 | |
| PCT/US2006/031214 WO2007032837A2 (en) | 2005-08-11 | 2006-08-11 | Method for in vitro recombination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1929012T3 true DK1929012T3 (da) | 2011-01-31 |
Family
ID=37734039
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10168787.9T DK2239327T3 (da) | 2005-08-11 | 2006-08-11 | Fremgangsmåde til in vitro rekombination |
| DK06836102.1T DK1929012T3 (da) | 2005-08-11 | 2006-08-11 | Fremgangsmåde til in vitro-rekombination |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10168787.9T DK2239327T3 (da) | 2005-08-11 | 2006-08-11 | Fremgangsmåde til in vitro rekombination |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US7776532B2 (da) |
| EP (2) | EP2239327B1 (da) |
| AT (1) | ATE483802T1 (da) |
| CA (1) | CA2618665C (da) |
| DE (1) | DE602006017405D1 (da) |
| DK (2) | DK2239327T3 (da) |
| MY (1) | MY144014A (da) |
| WO (1) | WO2007032837A2 (da) |
Families Citing this family (60)
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| US9273310B2 (en) | 2007-10-08 | 2016-03-01 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
| MY161418A (en) * | 2007-10-08 | 2017-04-14 | Synthetic Genomics Inc | Assembly of large nucleic acids |
| CN102016070A (zh) | 2008-02-15 | 2011-04-13 | 合成基因组公司 | 体外连接和组合装配核酸分子的方法 |
| US10093552B2 (en) | 2008-02-22 | 2018-10-09 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US9259662B2 (en) | 2008-02-22 | 2016-02-16 | James Weifu Lee | Photovoltaic panel-interfaced solar-greenhouse distillation systems |
| US8986963B2 (en) * | 2008-02-23 | 2015-03-24 | James Weifu Lee | Designer calvin-cycle-channeled production of butanol and related higher alcohols |
| US8735651B2 (en) * | 2008-02-23 | 2014-05-27 | James Weifu Lee | Designer organisms for photobiological butanol production from carbon dioxide and water |
| EP2282769A4 (en) | 2008-04-29 | 2012-04-25 | Abbott Lab | DUAL VARIABLE DOMAIN IMMUNOGLOBULINS AND ITS USES |
| CA2726087A1 (en) | 2008-06-03 | 2009-12-10 | Tariq Ghayur | Dual variable domain immunoglobulins and uses thereof |
| CN102124112B (zh) | 2008-09-10 | 2013-03-20 | 金斯瑞公司 | 基于同源重组dna的克隆方法及组合物 |
| US9234226B2 (en) * | 2008-10-14 | 2016-01-12 | Wayne M. Barnes | In vitro recombination methods using 5′ exonuclease |
| SG171760A1 (en) * | 2008-11-19 | 2011-07-28 | Amyris Inc | Compositions and methods for the assembly of polynucleotides |
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| US9981239B2 (en) | 2015-04-21 | 2018-05-29 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| KR20180050411A (ko) | 2015-09-18 | 2018-05-14 | 트위스트 바이오사이언스 코포레이션 | 올리고핵산 변이체 라이브러리 및 그의 합성 |
| CN113604546A (zh) | 2015-09-22 | 2021-11-05 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| EP3384077A4 (en) | 2015-12-01 | 2019-05-08 | Twist Bioscience Corporation | FUNCTIONALIZED SURFACES AND THEIR PREPARATION |
| GB2568444A (en) | 2016-08-22 | 2019-05-15 | Twist Bioscience Corp | De novo synthesized nucleic acid libraries |
| WO2018057526A2 (en) | 2016-09-21 | 2018-03-29 | Twist Bioscience Corporation | Nucleic acid based data storage |
| CN106701738B (zh) * | 2016-11-10 | 2020-05-08 | 中国科学院成都生物研究所 | 一种等温解开双链dna及制备单链dna的方法 |
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| GB2590196A (en) | 2018-05-18 | 2021-06-23 | Twist Bioscience Corp | Polynucleotides, reagents, and methods for nucleic acid hybridization |
| BR112021001473A2 (pt) | 2018-07-30 | 2021-04-27 | OriCiro Genomics, Inc. | método para edição de dna num sistema livre de células |
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| WO2021064115A1 (en) | 2019-10-01 | 2021-04-08 | Universitetet I Tromsø - Norges Arktiske Universitet | Method and kit for assembly of multiple dna fragments at room temperature |
| EP4041878A1 (en) | 2019-10-01 | 2022-08-17 | Universitetet i Tromsø - Norges Arktiske Universitet | Marine dna polymerase i |
| CN114729341A (zh) | 2019-10-01 | 2022-07-08 | 特罗姆瑟大学-挪威北极圈大学 | Dna聚合酶和dna聚合酶衍生的3′-5′外切核酸酶 |
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-
2006
- 2006-08-11 CA CA2618665A patent/CA2618665C/en active Active
- 2006-08-11 WO PCT/US2006/031214 patent/WO2007032837A2/en active Application Filing
- 2006-08-11 EP EP10168787.9A patent/EP2239327B1/en active Active
- 2006-08-11 EP EP06836102A patent/EP1929012B1/en active Active
- 2006-08-11 DE DE602006017405T patent/DE602006017405D1/de active Active
- 2006-08-11 MY MYPI20063911A patent/MY144014A/en unknown
- 2006-08-11 DK DK10168787.9T patent/DK2239327T3/da active
- 2006-08-11 US US11/502,624 patent/US7776532B2/en active Active
- 2006-08-11 AT AT06836102T patent/ATE483802T1/de not_active IP Right Cessation
- 2006-08-11 DK DK06836102.1T patent/DK1929012T3/da active
-
2010
- 2010-06-22 US US12/820,861 patent/US8435736B2/en active Active
-
2013
- 2013-04-16 US US13/864,108 patent/US20130295645A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US8435736B2 (en) | 2013-05-07 |
| EP1929012B1 (en) | 2010-10-06 |
| EP2239327B1 (en) | 2015-02-25 |
| CA2618665C (en) | 2012-11-13 |
| EP2239327A2 (en) | 2010-10-13 |
| CA2618665A1 (en) | 2007-03-22 |
| DE602006017405D1 (de) | 2010-11-18 |
| US20100311126A1 (en) | 2010-12-09 |
| WO2007032837A3 (en) | 2007-05-24 |
| US7776532B2 (en) | 2010-08-17 |
| EP2239327A3 (en) | 2012-04-25 |
| MY144014A (en) | 2011-07-29 |
| WO2007032837A2 (en) | 2007-03-22 |
| DK2239327T3 (da) | 2015-05-18 |
| EP1929012A2 (en) | 2008-06-11 |
| ATE483802T1 (de) | 2010-10-15 |
| US20070037196A1 (en) | 2007-02-15 |
| US20130295645A1 (en) | 2013-11-07 |
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