DK1149160T3 - Genvinding af et protein ved höjt pH - Google Patents

Genvinding af et protein ved höjt pH

Info

Publication number
DK1149160T3
DK1149160T3 DK00901052T DK00901052T DK1149160T3 DK 1149160 T3 DK1149160 T3 DK 1149160T3 DK 00901052 T DK00901052 T DK 00901052T DK 00901052 T DK00901052 T DK 00901052T DK 1149160 T3 DK1149160 T3 DK 1149160T3
Authority
DK
Denmark
Prior art keywords
elevated
protein
recovery
solution
state
Prior art date
Application number
DK00901052T
Other languages
English (en)
Inventor
Mads Aage Laustsen
Curran Matthew Simpson
Michael O'reilly
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1149160T3 publication Critical patent/DK1149160T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DK00901052T 1999-01-25 2000-01-24 Genvinding af et protein ved höjt pH DK1149160T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199900085 1999-01-25
DKPA199901491 1999-10-18
EP00901052A EP1149160B1 (en) 1999-01-25 2000-01-24 Recovery of a protein at high ph

Publications (1)

Publication Number Publication Date
DK1149160T3 true DK1149160T3 (da) 2006-08-14

Family

ID=26063274

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00901052T DK1149160T3 (da) 1999-01-25 2000-01-24 Genvinding af et protein ved höjt pH

Country Status (9)

Country Link
US (1) US6316240B1 (da)
EP (1) EP1149160B1 (da)
JP (1) JP2002534975A (da)
CN (1) CN1181197C (da)
AT (1) ATE323759T1 (da)
AU (1) AU2093100A (da)
DE (1) DE60027406T2 (da)
DK (1) DK1149160T3 (da)
WO (1) WO2000043502A1 (da)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008110498A1 (en) * 2007-03-15 2008-09-18 Novozymes A/S Solubilization of protease crystals in fermentation broth
BRPI0914982B1 (pt) * 2008-06-09 2019-09-24 Danisco Us Inc. Métodos para produção de uma formulação granular contendo enzima e grãnulo contendo enzima
GB0908770D0 (en) 2009-04-24 2009-07-01 Danisco Method
DK2914611T3 (da) * 2012-11-01 2018-12-10 Novozymes As Fremgangsmåde til fjernelse af DNA
MX2016012822A (es) * 2014-04-30 2016-12-09 Novozymes As Metodo para reducir el contenido de acido desoxirribonucleico (adn) de un caldo de fermentacion.
CN108367245A (zh) * 2015-12-09 2018-08-03 巴斯夫欧洲公司 在解吸条件下从发酵固体纯化蛋白质的方法
US20200109388A1 (en) * 2017-04-03 2020-04-09 Novozymes A/S Recovery Process
EP3784779A1 (en) 2018-04-26 2021-03-03 Basf Se Lipase enzymes
US20210115422A1 (en) 2018-05-03 2021-04-22 Basf Se Amylase enzymes
MX2021010110A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio.
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
US20220170001A1 (en) 2019-03-25 2022-06-02 Basf Se Amylase Enzymes
WO2020193532A1 (en) 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
US20220162576A1 (en) 2019-03-25 2022-05-26 Basf Se Amylase enzymes
EP3983425A1 (en) * 2019-06-13 2022-04-20 Basf Se Method of recovering a protein from fermentation broth using a divalent cation
US20220340865A1 (en) 2019-07-02 2022-10-27 Basf Se Method for preparing a fermentation medium
PL3959326T3 (pl) 2019-07-05 2023-10-09 Basf Se Przemysłowy sposób fermentacji dla komórek bakteryjnych z wykorzystaniem kultury wstępnej okresowej z zasilaniem
JP2022545465A (ja) 2019-08-22 2022-10-27 ビーエーエスエフ ソシエタス・ヨーロピア アミラーゼバリアント
EP4047088A1 (en) 2021-02-22 2022-08-24 Basf Se Amylase variants
MX2023009756A (es) 2021-02-22 2023-09-04 Basf Se Variantes de amilasa.
WO2024028340A1 (en) 2022-08-02 2024-02-08 Basf Se Stabilized protein production process using bacillus host cells with salt feed
WO2024033134A1 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising protease, mannanase, and/or cellulase
WO2024033136A1 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024033133A2 (en) 2022-08-11 2024-02-15 Basf Se Enzyme compositions comprising an amylase
WO2024033135A2 (en) 2022-08-11 2024-02-15 Basf Se Amylase variants
WO2024094732A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094735A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2024094733A1 (en) 2022-11-04 2024-05-10 Basf Se Polypeptides having protease activity for use in detergent compositions
EP4389864A1 (en) 2022-12-20 2024-06-26 Basf Se Cutinases

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2129810B (en) 1982-06-07 1985-09-18 Celltech Ltd A process for the preparation of chymosin
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
WO1984003711A1 (en) * 1983-03-25 1984-09-27 Celltech Ltd A process for the production of a protein
CA1267615A (en) 1984-08-27 1990-04-10 Dan Hadary Method for recovering purified growth hormones from genetically engineered microorganisms
JPH01222780A (ja) 1988-03-02 1989-09-06 Tosoh Corp ウロキナーゼ前駆体様蛋白質のリフォールディング方法
US5064943A (en) 1988-12-16 1991-11-12 American Cyanamid Company Method for solubilization and naturation of somatotropin
EP0574050A1 (en) 1992-05-19 1993-12-15 Gist-Brocades N.V. Large scale separation and purification of fermentation product
BE1009844A3 (fr) 1995-12-22 1997-10-07 Genencor Int Procede de recolte d'une proteine.

Also Published As

Publication number Publication date
WO2000043502A1 (en) 2000-07-27
JP2002534975A (ja) 2002-10-22
AU2093100A (en) 2000-08-07
CN1181197C (zh) 2004-12-22
EP1149160A1 (en) 2001-10-31
CN1337999A (zh) 2002-02-27
US6316240B1 (en) 2001-11-13
ATE323759T1 (de) 2006-05-15
DE60027406T2 (de) 2006-11-23
EP1149160B1 (en) 2006-04-19
DE60027406D1 (de) 2006-05-24

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