ATE557083T1 - Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität - Google Patents
Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivitätInfo
- Publication number
- ATE557083T1 ATE557083T1 AT10172676T AT10172676T ATE557083T1 AT E557083 T1 ATE557083 T1 AT E557083T1 AT 10172676 T AT10172676 T AT 10172676T AT 10172676 T AT10172676 T AT 10172676T AT E557083 T1 ATE557083 T1 AT E557083T1
- Authority
- AT
- Austria
- Prior art keywords
- thermostable
- attenuated
- dna polymerase
- exonuclease activity
- thermoactive dna
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36981502P | 2002-04-02 | 2002-04-02 |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE557083T1 true ATE557083T1 (de) | 2012-05-15 |
Family
ID=28042066
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT10172676T ATE557083T1 (de) | 2002-04-02 | 2003-03-31 | Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität |
Country Status (6)
Country | Link |
---|---|
US (1) | US7148049B2 (de) |
EP (2) | EP1350841B1 (de) |
JP (2) | JP4034684B2 (de) |
CN (1) | CN1482240B (de) |
AT (1) | ATE557083T1 (de) |
ES (1) | ES2389711T3 (de) |
Families Citing this family (44)
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JP2007159401A (ja) * | 2004-05-07 | 2007-06-28 | Precision System Science Co Ltd | 改変タンパク質の作製方法 |
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US7745125B2 (en) | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
US20070048748A1 (en) * | 2004-09-24 | 2007-03-01 | Li-Cor, Inc. | Mutant polymerases for sequencing and genotyping |
US7900380B2 (en) * | 2005-10-13 | 2011-03-08 | Masterfit Enterprises Inc. | User moldable adjustable insert |
US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
US8026091B2 (en) * | 2007-07-13 | 2011-09-27 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
CA2706999C (en) * | 2007-11-28 | 2013-01-22 | F. Hoffmann-La Roche Ag | Mutant dna polymerases with improved pyrophosphorolysis activated polymerization (pap) ability |
US10150990B2 (en) | 2008-04-21 | 2018-12-11 | Roche Molecular Systems, Inc. | Ribonucleotide tag nucleic acid detection |
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US9238832B2 (en) | 2009-12-11 | 2016-01-19 | Roche Molecular Systems, Inc. | Allele-specific amplification of nucleic acids |
GB201007384D0 (en) | 2010-04-30 | 2010-06-16 | Medical Res Council | Enzymes |
CN103025868B (zh) | 2010-06-18 | 2015-07-01 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
US8722380B2 (en) | 2010-06-18 | 2014-05-13 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
US8735121B2 (en) | 2010-06-18 | 2014-05-27 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-match discrimination |
ES2536978T3 (es) | 2010-06-18 | 2015-06-01 | F. Hoffmann-La Roche Ag | ADN polimerasas con discriminación incrementada de no correspondencias 3' |
CA2801997C (en) | 2010-06-18 | 2016-05-31 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
US8759062B2 (en) | 2010-06-18 | 2014-06-24 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′- mismatch discrimination |
WO2011157435A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
WO2011157430A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
US9617588B2 (en) | 2010-10-05 | 2017-04-11 | Thermo Fisher Scientific Baltics Uab | Enzyme mixture |
EP2675897B1 (de) | 2011-02-15 | 2016-05-11 | Roche Diagnostics GmbH | Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz |
ES2561885T3 (es) | 2011-04-11 | 2016-03-01 | F. Hoffmann-La Roche Ag | ADN polimerasas de actividad mejorada |
WO2012162621A1 (en) * | 2011-05-26 | 2012-11-29 | Brandeis University | Methods for suppression pcr |
ES2553400T3 (es) | 2011-07-28 | 2015-12-09 | F. Hoffmann-La Roche Ag | ADN polimerasas con actividad mejorada |
WO2013083262A1 (en) | 2011-12-08 | 2013-06-13 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
JP6140182B2 (ja) | 2011-12-08 | 2017-05-31 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 改善された活性を有するdnaポリメラーゼ |
CA2858264C (en) | 2011-12-08 | 2018-01-02 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
ES2713653T3 (es) | 2013-03-15 | 2019-05-23 | Ibis Biosciences Inc | Análogos nucleotídicos para secuenciación |
US20150050697A1 (en) | 2013-08-19 | 2015-02-19 | Abbott Molecular Inc. | Nucleotide analogs |
CN104152426A (zh) * | 2014-08-05 | 2014-11-19 | 孙启明 | 一种t5核酸外切酶在大肠杆菌中的制备方法 |
CN104313132A (zh) * | 2014-09-28 | 2015-01-28 | 南京诺唯赞生物科技有限公司 | 一种t4 dna聚合酶活性测定方法 |
GB2542391A (en) * | 2015-09-17 | 2017-03-22 | Annexin Pharmaceuticals Ab | Process of manufacture |
JP6934477B2 (ja) * | 2016-01-19 | 2021-09-15 | ボード オブ リージェンツ ザ ユニヴァーシティ オブ テキサス システム | 熱安定逆転写酵素 |
AU2017386515B2 (en) * | 2016-12-30 | 2021-08-19 | Pacific Biosciences Of California, Inc. | Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides |
WO2018148726A1 (en) * | 2017-02-13 | 2018-08-16 | Qiagen Waltham Inc. | Polymerase enzyme from phage t4 |
EP3580351A1 (de) | 2017-02-13 | 2019-12-18 | Qiagen Sciences, LLC | Polymeraseenzym aus 9°n |
CN118048419A (zh) * | 2017-10-04 | 2024-05-17 | 生捷科技控股公司 | 酶法合成寡核苷酸的方法和*** |
TWI680168B (zh) * | 2017-10-18 | 2019-12-21 | 環球晶圓股份有限公司 | 碳化矽晶片 |
US11866785B2 (en) | 2017-10-27 | 2024-01-09 | Board Of Regents, The University Of Texas System | Tumor specific antibodies and T-cell receptors and methods of identifying the same |
JP7218558B2 (ja) * | 2017-12-19 | 2023-02-07 | 東ソー株式会社 | 核酸増幅法および核酸増幅試薬 |
US20210032609A1 (en) | 2018-03-21 | 2021-02-04 | Roche Molecular Systems, Inc. | Dna polymerases for efficient and effective incorporation of methylated-dntps |
US11739306B2 (en) | 2018-09-13 | 2023-08-29 | Roche Molecular Systems, Inc. | Mutant DNA polymerase(s) with improved strand displacement ability |
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DK149867C (da) | 1979-08-08 | 1987-04-06 | New Zealand Dev Finance | Proteolytisk enzympraeparat og fremgangsmaade til fremstilling deraf |
US4399216A (en) | 1980-02-25 | 1983-08-16 | The Trustees Of Columbia University | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
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US4419446A (en) | 1980-12-31 | 1983-12-06 | The United States Of America As Represented By The Department Of Health And Human Services | Recombinant DNA process utilizing a papilloma virus DNA as a vector |
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EP0196864A3 (de) | 1985-03-25 | 1988-03-23 | Cetus Corporation | Behandlung und Sekretion von rekombinanten Proteinen mittels alkalischer Phosphatase, dazu verwendbare DNA-Sequenzen und mittels dieser Sequenzen transformierte Zellen |
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SG86311A1 (en) | 1997-04-10 | 2002-02-19 | Univ Singapore | Bacillus stearothermophilus dna polymerase i (klenow) clones with reduced 3'-to-5' exonuclease activity |
AU7933898A (en) * | 1997-06-26 | 1999-01-19 | Takara Shuzo Co., Ltd. | Dna polymerase-related factors |
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US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
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-
2003
- 2003-03-26 US US10/401,403 patent/US7148049B2/en active Active
- 2003-03-31 EP EP20030006888 patent/EP1350841B1/de not_active Expired - Lifetime
- 2003-03-31 ES ES10172676T patent/ES2389711T3/es not_active Expired - Lifetime
- 2003-03-31 EP EP10172676A patent/EP2272952B8/de not_active Expired - Lifetime
- 2003-03-31 AT AT10172676T patent/ATE557083T1/de active
- 2003-04-02 JP JP2003099762A patent/JP4034684B2/ja not_active Expired - Lifetime
- 2003-04-02 CN CN031312330A patent/CN1482240B/zh not_active Expired - Lifetime
-
2006
- 2006-08-24 JP JP2006228285A patent/JP4585996B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP2006311867A (ja) | 2006-11-16 |
CN1482240B (zh) | 2012-09-05 |
JP4585996B2 (ja) | 2010-11-24 |
US20040005599A1 (en) | 2004-01-08 |
ES2389711T3 (es) | 2012-10-30 |
EP2272952B8 (de) | 2012-08-15 |
EP1350841A3 (de) | 2003-12-10 |
US7148049B2 (en) | 2006-12-12 |
JP4034684B2 (ja) | 2008-01-16 |
EP1350841A2 (de) | 2003-10-08 |
EP2272952A1 (de) | 2011-01-12 |
JP2003304870A (ja) | 2003-10-28 |
CN1482240A (zh) | 2004-03-17 |
EP1350841B1 (de) | 2015-05-06 |
EP2272952B1 (de) | 2012-05-09 |
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