DK0398539T3 - Subtilisiner med flere mutationer - Google Patents

Subtilisiner med flere mutationer

Info

Publication number
DK0398539T3
DK0398539T3 DK90304715.7T DK90304715T DK0398539T3 DK 0398539 T3 DK0398539 T3 DK 0398539T3 DK 90304715 T DK90304715 T DK 90304715T DK 0398539 T3 DK0398539 T3 DK 0398539T3
Authority
DK
Denmark
Prior art keywords
subtilisins
multiple mutations
subtilisin
admixture
expressing
Prior art date
Application number
DK90304715.7T
Other languages
English (en)
Inventor
Mark M Zukowski
Linda O Narhi
Michael Levitt
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of DK0398539T3 publication Critical patent/DK0398539T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cosmetics (AREA)
  • Tires In General (AREA)
DK90304715.7T 1989-05-17 1990-05-01 Subtilisiner med flere mutationer DK0398539T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35312489A 1989-05-17 1989-05-17

Publications (1)

Publication Number Publication Date
DK0398539T3 true DK0398539T3 (da) 1996-12-09

Family

ID=23387862

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90304715.7T DK0398539T3 (da) 1989-05-17 1990-05-01 Subtilisiner med flere mutationer

Country Status (14)

Country Link
US (1) US5397705A (da)
EP (1) EP0398539B1 (da)
JP (1) JPH03505976A (da)
KR (1) KR100188532B1 (da)
AT (1) ATE141324T1 (da)
AU (1) AU618675B2 (da)
CA (1) CA2016211C (da)
DE (1) DE69028040T2 (da)
DK (1) DK0398539T3 (da)
ES (1) ES2091224T3 (da)
GR (1) GR3021010T3 (da)
HK (1) HK1006857A1 (da)
SG (1) SG47797A1 (da)
WO (1) WO1990014420A1 (da)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE184316T1 (de) * 1987-04-06 1999-09-15 Novo Nordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
HUT66846A (en) * 1991-10-16 1995-01-30 Unilever Nv Aqueous enzymatic detergent compositions
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
AU8079794A (en) * 1993-10-14 1995-05-04 Procter & Gamble Company, The Protease-containing cleaning compositions
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
DE69432954T2 (de) * 1993-10-14 2004-04-22 The Procter & Gamble Company, Cincinnati Proteasen enthaltende bleichmittelzusammensetzungen
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
CA2273851C (en) * 1996-12-20 2007-04-10 Unilever Plc Enzymatic bleach composition
US6204234B1 (en) * 1997-07-09 2001-03-20 The Proctor & Gamble Company Cleaning compositions comprising a specific oxygenase
MA25044A1 (fr) * 1997-10-23 2000-10-01 Procter & Gamble Compositions de lavage contenant des variants de proteases multisubstituees.
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
CA2325568A1 (en) 1998-03-26 1999-09-30 The Procter & Gamble Company Serine protease variants having amino acid substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
MXPA02000840A (es) 1999-07-22 2002-07-30 Procter & Gamble Variantes de proteasa de subtilisina que tienen substituciones de aminoacidos en regiones de epitopes definidas.
KR20020021395A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 입체적으로 보호된 ｃｌｉｐ 부위를 갖는 프로테아제콘쥬게이트
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
EP1206526A2 (en) 1999-07-22 2002-05-22 The Procter & Gamble Company Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
WO2001016285A2 (en) * 1999-08-31 2001-03-08 Novozymes A/S Novel proteases and variants thereof
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
AU2001280968A1 (en) * 2000-07-31 2002-02-13 Menzel, Rolf Compositions and methods for directed gene assembly
ES2309167T3 (es) 2001-02-19 2008-12-16 Merck Patent Gmbh Metodo para identificar epitotes de celulas t y metodo para preparar moleculas con inmunogenicidad reducida.
EP1373296B1 (en) * 2001-03-23 2011-10-05 Genencor International, Inc. Proteins producing an altered immunogenic response and methods of making and using the same
AU2002367465A1 (en) * 2001-07-09 2003-09-29 Children's Hospital Medical Center Of Akron Multiple controls for molecular genetic analyses
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
AU2003210551A1 (en) * 2002-01-16 2003-09-02 Genencor International, Inc. Multiply-substituted protease variants
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) * 2003-03-21 2010-06-01 Novozymes A/S Subtilases
ATE407202T1 (de) * 2003-05-07 2008-09-15 Novozymes As Enzymvarianten von subtilisin (subtilasen)
EP3887515A1 (en) * 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
IE65767B1 (en) * 1986-04-30 1995-11-15 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
EP0479396B1 (en) * 1987-02-27 1999-06-09 Genencor International, Inc. Transformation of alkalophilic bacillus strains
ATE184316T1 (de) * 1987-04-06 1999-09-15 Novo Nordisk As Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
FR2614467A1 (fr) * 1987-04-21 1988-10-28 Telemecanique Electrique Appareil interrupteur de protection a mecanisme de declenchement simplifie

Also Published As

Publication number Publication date
EP0398539A1 (en) 1990-11-22
WO1990014420A1 (en) 1990-11-29
KR100188532B1 (ko) 1999-06-01
EP0398539B1 (en) 1996-08-14
DE69028040D1 (de) 1996-09-19
KR920701426A (ko) 1992-08-11
HK1006857A1 (en) 1999-03-19
ATE141324T1 (de) 1996-08-15
US5397705A (en) 1995-03-14
AU618675B2 (en) 1992-01-02
ES2091224T3 (es) 1996-11-01
SG47797A1 (en) 1998-04-17
GR3021010T3 (en) 1996-12-31
CA2016211A1 (en) 1990-11-17
DE69028040T2 (de) 1997-02-27
AU5558090A (en) 1990-12-18
JPH03505976A (ja) 1991-12-26
CA2016211C (en) 1998-07-21

Similar Documents

Publication Publication Date Title
DK0398539T3 (da) Subtilisiner med flere mutationer
DE3853328D1 (de) Subtilisin-analoge.
ES2125224T3 (es) Mutantes de subtilisina.
ATE82281T1 (de) Aryl-substituierte(n-piperidinyl)methyl und (npiperazinyl)methylazole mit antipsychotischer wirkung.
HUT72325A (en) Stabile, liophylised composition containing proteins and kit applicabe for dosing
HU9600960D0 (en) Protease-containing cleaning compositions
TR24655A (tr) Karbon isleri ve karbon islerini iceren kaucuk bilesimleri
DE3469916D1 (en) Compositions containing odor purified proteolytic enzymes and perfumes
DE69415659D1 (de) Oxidativ stabile alpha-amylase
ES2090857T3 (es) Composiciones para el cuidado del cabello.
DE69312499D1 (de) Kautschukzusammensetzungen enthaltend epoxidierten naturkautschuk und naturkautschuk
ATE110772T1 (de) Mutierter t-pa mit kringle-ersatz.
DE69009795D1 (de) Muramylpeptid-Derivate und diese enthaltende immunregulierende Zusammensetzungen.
FI863078A (fi) Kontinuerligt frigoerande peptidkompositioner.
ES2058764T3 (es) Composiciones orales.
DE69211770D1 (de) Haarbehandlungsmittel mit Perfluoropolyethern und einem Perlglanzmittel
IL85239A0 (en) Recombinant alveolar surfactant protein,its production and purification and pharmaceutical compositions containing it
RO103459B1 (en) Rubber composition for garnitures of bellows type
BG60657B2 (en) Human recombinant interleukin-2 muteins