DE69933579D1 - Serum-freies medium für chondrozyt-ähnliche zellen - Google Patents

Serum-freies medium für chondrozyt-ähnliche zellen

Info

Publication number
DE69933579D1
DE69933579D1 DE69933579T DE69933579T DE69933579D1 DE 69933579 D1 DE69933579 D1 DE 69933579D1 DE 69933579 T DE69933579 T DE 69933579T DE 69933579 T DE69933579 T DE 69933579T DE 69933579 D1 DE69933579 D1 DE 69933579D1
Authority
DE
Germany
Prior art keywords
serum
free medium
chondrocytes
chondrocyte
free
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69933579T
Other languages
English (en)
Inventor
Ranieri Cancedda
Beatrice Dozin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Istituto Nazionale per la Ricerca Sul Cancro
Consorzio per la Gestione del Centro di Biotecnologia Avanzata
Original Assignee
Istituto Nazionale per la Ricerca Sul Cancro
Consorzio per la Gestione del Centro di Biotecnologia Avanzata
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Istituto Nazionale per la Ricerca Sul Cancro, Consorzio per la Gestione del Centro di Biotecnologia Avanzata filed Critical Istituto Nazionale per la Ricerca Sul Cancro
Application granted granted Critical
Publication of DE69933579D1 publication Critical patent/DE69933579D1/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0655—Chondrocytes; Cartilage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/105—Insulin-like growth factors [IGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/135—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Rheumatology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE69933579T 1998-11-09 1999-11-08 Serum-freies medium für chondrozyt-ähnliche zellen Expired - Fee Related DE69933579D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10764698P 1998-11-09 1998-11-09
PCT/EP1999/008482 WO2000027996A1 (en) 1998-11-09 1999-11-08 Serum free medium for chondrocyte-like cells

Publications (1)

Publication Number Publication Date
DE69933579D1 true DE69933579D1 (de) 2006-11-23

Family

ID=22317692

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69933579T Expired - Fee Related DE69933579D1 (de) 1998-11-09 1999-11-08 Serum-freies medium für chondrozyt-ähnliche zellen

Country Status (10)

Country Link
US (2) US6617159B1 (de)
EP (1) EP1131407B1 (de)
JP (1) JP2002529071A (de)
AT (1) ATE342348T1 (de)
AU (1) AU758073B2 (de)
CA (1) CA2348687A1 (de)
DE (1) DE69933579D1 (de)
IL (2) IL142914A0 (de)
RU (1) RU2272839C2 (de)
WO (1) WO2000027996A1 (de)

Families Citing this family (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2395271A1 (en) * 1999-12-28 2001-07-05 Isotis N.V. Cell culture medium containing growth factors and l-glutamine
CA2444959C (en) 2001-04-24 2014-07-08 Dolores Baksh Progenitor cell populations, expansion thereof, and growth of non-hematopoietic cell types and tissues therefrom
FR2828401B1 (fr) * 2001-08-10 2003-11-07 Oreal Composition cosmetique ou dermatologique comprenant une association entre l'igf1 et/ou un compose mimetique de l'igf1, et l'acide ascorbique et/ou au moins l'un de ses derives
JP2003052360A (ja) * 2001-08-20 2003-02-25 Japan Science & Technology Corp 基底膜細胞外基質を用いた間葉系幹細胞の培養方法
CA2468335C (en) 2001-12-07 2014-07-08 Geron Corporation Chondrocyte precursors derived from human embryonic stem cells
US20070141036A1 (en) * 2002-01-09 2007-06-21 Alberto Gorrochategui Barrueta Composition and procedure for tissue creation, regeneration and repair by a cell-bearing biological implant enriched with platelet concentrate and supplements
WO2003064598A2 (en) 2002-01-25 2003-08-07 Genzyme Corporation Serum-free media for chondrocytes and methods of use thereof
US7741116B2 (en) * 2002-03-06 2010-06-22 University Of Cincinnati Surgical device for skin therapy or testing
AU2003220966A1 (en) * 2002-04-03 2003-10-13 Naohide Yamashita Drug containing human placenta-origin mesenchymal cells and process for producing vegf using the cells
EP1538200B1 (de) * 2002-05-22 2017-04-05 Matsutani Chemical Industry Co., Ltd. Verfahren zur verwendung der physiologischen aktivität von seltenen sacchariden und zusammensetzungen enthaltend seltenen sacchariden
DE10311620A1 (de) * 2003-03-17 2004-10-07 Biotissue Technologies Gmbh Knorpelzell-Kulturmedium und Verwendung desselben
AU2003903896A0 (en) 2003-07-28 2003-08-07 Queensland University Of Technology Skin regeneration system
WO2005065354A2 (en) * 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
US20080113434A1 (en) * 2004-03-05 2008-05-15 Davies John E Serum-Free Suspension Culture System For Mesenchymal Progenitor Cells
WO2005099758A2 (en) * 2004-04-17 2005-10-27 The Board Of Trustees The Leland Standford Junior University Injectable bioartificial tissue matrix
WO2005113749A2 (en) * 2004-05-14 2005-12-01 Becton, Dickinson And Company Stem cell populations and methods of use
JP4219955B2 (ja) * 2004-07-06 2009-02-04 協和発酵キリン株式会社 神経細胞の製造方法
US8697139B2 (en) 2004-09-21 2014-04-15 Frank M. Phillips Method of intervertebral disc treatment using articular chondrocyte cells
JP4646600B2 (ja) * 2004-11-02 2011-03-09 オリンパス株式会社 間葉系幹細胞の培養方法
JP2006136281A (ja) * 2004-11-15 2006-06-01 Olympus Corp 培地および間葉系幹細胞の培養方法
KR20070060148A (ko) 2004-11-19 2007-06-12 가부시끼가이샤 제이엠에스 세포배양용 인간 혈청
US7972767B2 (en) 2005-05-09 2011-07-05 Olympus Corporation Method for culturing mesenchymal stem cell and method for producing biological tissue prosthesis
US20090169523A1 (en) 2005-06-13 2009-07-02 Catherine Verfaillie Hsc self-renewal
US8728814B2 (en) 2005-08-23 2014-05-20 Oriental Yeast Co., Ltd. Technique for culture of mesenchymal stem cell utilizing laminin-5
JP4921083B2 (ja) * 2005-09-13 2012-04-18 タカラバイオ株式会社 レトロウィルス産生用無血清培地
US7989205B2 (en) * 2005-10-06 2011-08-02 American Cryostem Corporation Cell culture media, kits and methods of use
DE102005054577A1 (de) * 2005-11-16 2007-05-24 Cognis Ip Management Gmbh Verwendung von Estern ungesättigter, physiologisch aktiver Fettsäuren als Nährmedien für Zellkulturen
EP1988159B1 (de) 2006-01-13 2014-03-05 Two Cells Co., Ltd. Zusatzstoff für kulturmedium zur verwendung bei der serumfreien kultur einer tierzelle, kit und verwendung des zusatzstoffs oder kits
ITTO20060282A1 (it) * 2006-04-14 2007-10-15 Univ Degli Studi Torino Mezzo di coltura e composizione farmaceutica per la rigenerazione del tessuto cartilagineo relativo procedimento relativi usi e prodotti
BRPI0713572A2 (pt) * 2006-06-20 2012-10-23 Stephen Duguay meios livres de soro e seus usos para expansão de condrócitos
EP1900810A1 (de) * 2006-09-15 2008-03-19 Celogos Verfahren zur Zellextraktion und Zellselektion
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
US7951593B2 (en) * 2007-03-20 2011-05-31 Universite Rene Descartes-Paris V Culture medium for gingival fibroblasts
US8114668B2 (en) 2007-05-14 2012-02-14 Cardiac Pacemakers, Inc. Composition for cold storage of stem cells
ITMI20071421A1 (it) * 2007-07-16 2009-01-17 Fond Irccs Istituto Di Ricove Coltura per espandere cellule staminali ex-vivo
WO2009030092A1 (en) * 2007-09-05 2009-03-12 Institute Of Basic Medical Sciences Chinese Academy Of Medical Sciences Culture medium and method for in vitro culturing human adult primary mesenchymal stem cells on a large scale, primary mesenchymal stem cells obtained by the method, the uses thereof
WO2009092092A1 (en) 2008-01-18 2009-07-23 Regents Of The University Of Minnesota Stem cell aggregates and methods for making and using
US20100015710A1 (en) * 2008-04-25 2010-01-21 Sunghoon Jung Methods and Compositions for Isolating, Maintaining and Serially Expanding Human Mesenchymal Stem Cells
CA2729121C (en) * 2008-06-30 2019-04-09 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
KR101712501B1 (ko) 2008-11-11 2017-03-07 고쿠리츠다이가쿠호진 히로시마다이가쿠 분화 유도 배지용 첨가제 및 그의 용도
WO2011011477A1 (en) 2009-07-21 2011-01-27 Abt Holding Company Use of stem cells to reduce leukocyte extravasation
BR112012001329B1 (pt) 2009-07-21 2021-04-13 Abt Holding Company Uso de células progenitoras adultas multipotentes (mapcs), e, métodos para obter células progenitoras adultas multipotentes (mapcs) com uma potência desejada e para aumentar a potência de células progenitoras adultas multipotentes (mapcs)
SG10201403202XA (en) * 2009-07-23 2014-08-28 Agency Science Tech & Res Pre-natal mesenchymal stem cells
CA3174901A1 (en) * 2009-11-12 2011-05-19 Technion Research & Development Foundation Ltd. Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
IL282662B2 (en) 2010-02-25 2024-01-01 Univ Case Western Reserve Modulation of macrophage activation
SG183498A1 (en) 2010-02-25 2012-09-27 Abt Holding Co Modulation of angiogenesis
US9394521B2 (en) 2010-03-10 2016-07-19 Two Cells Co., Ltd. Cell preparation containing mesenchymal stem cells, and method for producing same
US9029145B2 (en) 2010-04-08 2015-05-12 The University Court Of The University Of Edinburgh Chondrogenic progenitor cells, protocol for derivation of cells and uses thereof
SG10201913920PA (en) 2010-05-12 2020-03-30 Abt Holding Co Modulation of splenocytes in cell therapy
WO2011158125A2 (en) 2010-06-17 2011-12-22 Katholieke Universiteit Leuven Methods for differentiating cells into hepatic stellate cells and hepatic sinusoidal endothelial cells, cells produced by the methods, and methods for using the cells
AU2011293440B2 (en) 2010-08-24 2016-05-05 Katholieke Universiteit Leuven Non-static suspension culture of cell aggregates
WO2012048275A2 (en) 2010-10-08 2012-04-12 Caridianbct, Inc. Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
EP2670842A2 (de) 2011-01-31 2013-12-11 Katholieke Universiteit Leuven KU Leuven Research & Development Verfahren zur herstellung von zellen mit einem embryonenexternen endodermalen vorläuferphänotyp
ES2763331T3 (es) 2011-06-06 2020-05-28 ReGenesys BVBA Expansión de células madre en biorreactores de fibra hueca
RU2461623C1 (ru) * 2011-06-20 2012-09-20 Борис Карпович Гаврилюк Способ выращивания культур хрящевых клеток
JP2014521337A (ja) 2011-07-29 2014-08-28 セルラー ダイナミクス インターナショナル， インコーポレイテッド 幹細胞由来の組織細胞中の代謝成熟
JP2014526887A (ja) 2011-08-01 2014-10-09 アルナイラム ファーマシューティカルズ， インコーポレイテッド 造血幹細胞移植の成功率を改善する方法
GB201119335D0 (en) 2011-11-09 2011-12-21 Univ Leuven Kath Hepatitis virus infectable stem cells
US9321995B2 (en) * 2012-12-20 2016-04-26 Suzhou Biowisetech Co., Ltd. Stem cell culture medium and its applications as well as a stem cell culture method
WO2014146057A2 (en) * 2013-03-15 2014-09-18 Cmr Technologies, Llc Mesenchymal stem cells
SG11201508403XA (en) 2013-04-12 2015-11-27 Saverio Lafrancesca Improving organs for transplantation
EP2992088B1 (de) 2013-04-30 2019-08-21 Katholieke Universiteit Leuven Zelltheraphie für myelodysplastische syndrome
JP6512759B2 (ja) * 2013-08-19 2019-05-15 国立研究開発法人国立循環器病研究センター 羊膜間葉系細胞組成物の製造方法及び凍結保存方法、並びに治療剤
CN105765059B (zh) * 2013-11-04 2021-05-25 伊索波根控股公司 细胞培养方法
WO2015073913A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
CN103805562B (zh) * 2014-01-13 2015-08-05 章毅 培养胎盘间充质干细胞的无血清培养基
US11008547B2 (en) 2014-03-25 2021-05-18 Terumo Bct, Inc. Passive replacement of media
AU2015264043B2 (en) * 2014-05-23 2020-08-13 Regen Therapies, Inc. Formulation for regeneration of bone, cartilage, teeth, and periodontium and treatment of tumors and cysts
US9433629B2 (en) 2014-05-23 2016-09-06 Juan Paz Garcia Formulation for regeneration of bone, cartilage, teeth, and periodontium and treatment of tumors and cysts
US9669074B2 (en) * 2014-05-23 2017-06-06 Juan Paz Garcia Formulation for regeneration of bone, cartilage, teeth, and periodontium and treatment of tumors and cysts
WO2016049421A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled feed
AU2015346672B2 (en) * 2014-11-14 2017-09-21 Regenesis Science Co., Ltd. Method for serum-free culture of cartilage cells and serum-free culture medium
AU2016227607B2 (en) * 2015-03-04 2021-09-30 Mesoblast International Sarl Cell culture method for mesenchymal stem cells
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
NZ745530A (en) 2016-01-21 2023-03-31 Abt Holding Co Stem cells for wound healing
EP3464565A4 (de) 2016-05-25 2020-01-01 Terumo BCT, Inc. Zellexpansion
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
SG11201900058YA (en) 2016-07-04 2019-02-27 Agency Science Tech & Res Method of generating mesenchymal stem cells and uses thereof
EP3656842A1 (de) 2017-03-31 2020-05-27 Terumo BCT, Inc. Zellexpansion
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CA3078759A1 (en) 2017-10-13 2019-04-18 Boehringer Ingelheim International Gmbh Perfusion medium
JP7372518B2 (ja) * 2019-06-27 2023-11-01 国立大学法人山口大学 骨髄由来間葉系幹細胞の培養方法
US20230083026A1 (en) * 2020-02-03 2023-03-16 Mosa Meat B.V. Serum-free medium for culturing a bovine progenitor cell
JP2024511064A (ja) 2021-03-23 2024-03-12 テルモ ビーシーティー、インコーポレーテッド 細胞捕獲及び増殖

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5902741A (en) * 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US5405772A (en) * 1993-06-18 1995-04-11 Amgen Inc. Medium for long-term proliferation and development of cells
US5908782A (en) 1995-06-05 1999-06-01 Osiris Therapeutics, Inc. Chemically defined medium for human mesenchymal stem cells
WO1996040866A1 (en) 1995-06-07 1996-12-19 Novartis Ag Serum-free media for primitive hematopoietic cells and methods of use thereof
CA2248142A1 (en) 1996-03-12 1997-09-18 Life Technologies, Inc. Hematopoietic cell culture nutrient supplement
JP3638614B2 (ja) 1996-07-25 2005-04-13 ジェンザイム・コーポレーション 軟骨細胞培地組成および培養方法
WO1998032333A1 (en) * 1996-12-06 1998-07-30 Osiris Therapeutics, Inc. Improved chondrogenic differentiation of human mesenchymal stem cells

Also Published As

Publication number Publication date
US6617159B1 (en) 2003-09-09
EP1131407B1 (de) 2006-10-11
AU758073B2 (en) 2003-03-13
IL142914A (en) 2006-07-05
US7109032B2 (en) 2006-09-19
RU2272839C2 (ru) 2006-03-27
US20050090002A1 (en) 2005-04-28
CA2348687A1 (en) 2000-05-18
AU1380400A (en) 2000-05-29
EP1131407A1 (de) 2001-09-12
WO2000027996A1 (en) 2000-05-18
ATE342348T1 (de) 2006-11-15
JP2002529071A (ja) 2002-09-10
IL142914A0 (en) 2002-04-21

Similar Documents

Publication Publication Date Title
DE69933579D1 (de) Serum-freies medium für chondrozyt-ähnliche zellen
RU2001116126A (ru) Бессывороточная среда для хондроцитоподобных клеток
IL168984A (en) Methods and culture systems for preparing feeder cells - free, xeno - free human embryonic stem cells and stem cell cultures prepared using same
WO1998059035A3 (en) Serum-free cell growth medium
WO2006047380A3 (en) Method and media for single cell serum-fee culture of cho cells
FR2726003B1 (fr) Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
DK1918364T3 (da) Fremgangsmåde til rekloning af produktionsceller
GB2432846A (en) Medium and culture of embryonic stem cells
WO2002072762A3 (en) Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture
WO2006045064A3 (en) Cultured hematopoietic stem cells and method for expansion and analysis thereof
WO2003064598A3 (en) Serum-free media for chondrocytes and methods of use thereof
JP2003525625A5 (de)
WO2003014313A3 (en) Alternative compositions and methods for the culture of stem cells
CN103068969A (zh) 化学成分确定的无血清细胞培养基
ATE399855T1 (de) Tierzellkulturmedium mit pflanzlichen nährstoffen
JPH0387174A (ja) 細胞培養基
WO2000017321A3 (en) Use of growth factors and hormones for expansion of mammalian cells and tissue engineering
JP2013510567A5 (de)
WO1998004681A3 (en) Chondrocyte media formulations and culture procedures
EP0953354A4 (de) Hämatopoietische stammzellen proliferierende wirkstoffe
NO20033396D0 (no) Forbedret in vitro fremgangsmåte for kultivering av mammale celler for autolog celle implantasjon/transplantasjon fremgangsmåter
ATE295875T1 (de) Verfahren zur in vitro herstellung von knochen
DE69029856D1 (de) Verfahren, zubereitungen und anordnungen für zellzucht
NZ502712A (en) Medium and method for long term culturing of avian primordial germ cells (PGC) in a tissue culture comprising growth factors
WO1999067360A3 (en) Media and methods for expansion of erythroid stem cells for the production of hemoglobin

Legal Events

Date Code Title Description
8339 Ceased/non-payment of the annual fee