SG11201900058YA - Method of generating mesenchymal stem cells and uses thereof - Google Patents

Method of generating mesenchymal stem cells and uses thereof

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Publication number
SG11201900058YA
SG11201900058YA SG11201900058YA SG11201900058YA SG11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA SG 11201900058Y A SG11201900058Y A SG 11201900058YA
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Singapore
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singapore
international
cells
growth factor
genome
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SG11201900058YA
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tong ming Liu
Bing Lim
Pin Li
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Agency Science Tech & Res
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Publication of SG11201900058YA publication Critical patent/SG11201900058YA/en

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    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
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Abstract

Mesoderm FOXF+- Handl+ APLNR1 MSC CD14-, CD34-, CD45-, CO29+, CD44+, C049c+, 0373+, CD105+, CD151+, CD166+ (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 11 January 2018 (11.01.2018) WIP0 I PCT onion OH oI0IIIIIIII1101HDIDMDlII111111 DIIIIII (10) International Publication Number WO 2018/009147 Al (51) International Patent Classification: C12N 5/0775 (2010.01) A61P 19/04 (2006.01) A61K 35/28 (2015.01) (21) International Application Number: PCT/SG2017/050339 (22) International Filing Date: 04 July 2017 (04.07.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 10201605481T 04 July 2016 (04.07.2016) SG (71) Applicant: AGENCY FOR SCIENCE, TECHNOLO- GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 Connexis North Tower, Singapore 138632 (SG). (72) Inventors: LIU, Tong Ming; c/o Genome Institute of Sin- gapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). LIM, Bing; c/o Genome Institute of Sin- gapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). LI, Pin; c/o Genome Institute of Singapore, 60 Biopolis Street, Genome Building, Singapore 138672 (SG). (74) Agent: SPRUSON & FERGUSON (ASIA) PTE LTD; P.O. Box 1531, Robinson Road Post Office, Singapore 903031 (SG). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: — of inventorship (Rule 4.17(iv)) Published: — with international search report (Art. 21(3)) = (54) Title: METHOD OF GENERATING MESENCHYMAL STEM CELLS AND USES THEREOF 1-1 N 1-1 t:T O O GC O 1-1 N O ACF FBRFs FPEVc do di d2 Stage 1 d10 Stage 2 d21 Stage 3 (57) : The present disclosure provides a method of generating mature mesenchymal stem cells (MSC) from lateral plate mesoderm comprising culturing the lateral plate mesoderm cells on an extracellular matrix in a MSC cell culture media comprising (i) fibroblast growth factor; (ii) platelet-derived growth factor (PDGF); (iii) epidermal growth factor (EGF) and (iv) ascorbic acid. Also claimed are MSCs obtained by said culture method and a method of treating cartilage and bone disease in a patient using said cells. In addition, a culture medium for deriving primitive streak mesendoderm cells from pluripotent stem cells comprising (a) activin; (b) WNT- signalling activator; and/or (c) fibroblast growth factor and a culture medium for deriving lateral plate mesoderm cells from primitive streak mesendoderm cells comprising (1) fibroblast growth factor; (2) bone morphogenetic protein; (3) follistatin; and optionally Rho- associated protein kinase (ROCK) inhibitor are claimed.
SG11201900058YA 2016-07-04 2017-07-04 Method of generating mesenchymal stem cells and uses thereof SG11201900058YA (en)

Applications Claiming Priority (2)

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SG10201605481T 2016-07-04
PCT/SG2017/050339 WO2018009147A1 (en) 2016-07-04 2017-07-04 Method of generating mesenchymal stem cells and uses thereof

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SG11201900058YA true SG11201900058YA (en) 2019-02-27

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CN111019889A (en) * 2019-12-25 2020-04-17 依科赛生物科技（太仓）有限公司 Mesenchymal stem cell chemical component-limited serum-free culture medium and application thereof
CN111166884B (en) * 2020-01-15 2021-12-03 广州中医药大学第一附属医院 Application of Foxf1 gene in preparation of medicine for treating osteoporosis
FR3107709B1 (en) * 2020-02-28 2023-09-15 Ic Biosolutions METHOD FOR PRODUCING MESENCHYMAL STEM CELLS COMPETENT FOR CARTILAGE REGENERATION FROM EQUINE PLURIPOTENT STEM CELLS
CN112870361A (en) * 2021-01-28 2021-06-01 重庆医科大学 Use of iron death inhibitor for preparing medicine for preventing or treating osteoporosis or bone loss caused by iron overload
WO2022251537A1 (en) * 2021-05-26 2022-12-01 University Of Southern California Production processes for highly viable, engraftable human chondrocytes from stem cells
CN114480266A (en) * 2022-01-21 2022-05-13 中山大学附属第三医院 Composition for improving homogeneity and reducing immunogenicity of mesenchymal stem cells
WO2023148556A1 (en) * 2022-02-07 2023-08-10 Smartcella Solutions Ab Methods and compositions for generating human induced mesenchymal stem cells
CN114672455A (en) * 2022-03-25 2022-06-28 中山大学 Method for inducing bone marrow stromal cells by utilizing pluripotent stem cells
CN114891737B (en) * 2022-04-25 2023-05-12 中山大学 Preparation method and application of multipotent stem cell-derived kidney mesenchymal stem cells

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AU758073B2 (en) 1998-11-09 2003-03-13 Consorzio Per La Gestione Del Centro Di Biotecnologie Avanzate Serum free medium for chondrocyte-like cells
US9175260B2 (en) * 2007-01-30 2015-11-03 TheUniversity of Georgia Research Foundation, Inc. Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells (MMC)
US20110236971A2 (en) 2007-09-25 2011-09-29 Maksym Vodyanyk Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions
AU2010306377B2 (en) * 2009-10-13 2015-01-22 Stemcell Technologies Canada Inc. Manipulation of osmolality for differentiating stem cells
EP2598634A1 (en) * 2010-07-26 2013-06-05 The University Of Manchester Targeted differentiation of stem cells

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US20190322987A1 (en) 2019-10-24
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