DE69732378D1 - Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae - Google Patents

Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae

Info

Publication number
DE69732378D1
DE69732378D1 DE69732378T DE69732378T DE69732378D1 DE 69732378 D1 DE69732378 D1 DE 69732378D1 DE 69732378 T DE69732378 T DE 69732378T DE 69732378 T DE69732378 T DE 69732378T DE 69732378 D1 DE69732378 D1 DE 69732378D1
Authority
DE
Germany
Prior art keywords
production
expression vectors
saccharomyces cerevisiae
highly efficient
recombinant proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69732378T
Other languages
English (en)
Inventor
Ki-Ryong Jang
Jae-Woong Moon
Cheon-Soon Bae
Doo-Suk Yang
Jee-Won Lee
Baik-Lin Seong
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hanil Synthetic Fiber Co Ltd
Original Assignee
Hanil Synthetic Fiber Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanil Synthetic Fiber Co Ltd filed Critical Hanil Synthetic Fiber Co Ltd
Application granted granted Critical
Publication of DE69732378D1 publication Critical patent/DE69732378D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE69732378T 1997-05-27 1997-05-27 Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae Expired - Lifetime DE69732378D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/KR1997/000097 WO1998054339A1 (en) 1997-05-27 1997-05-27 PROCESS FOR PREPARING RECOMBINANT PROTEINS USING HIGHLY EFFICIENT EXPRESSION VECTOR FROM $i(SACCHAROMYCES CEREVISIAE)

Publications (1)

Publication Number Publication Date
DE69732378D1 true DE69732378D1 (de) 2005-03-03

Family

ID=19494122

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69732378T Expired - Lifetime DE69732378D1 (de) 1997-05-27 1997-05-27 Verfahren zur herstellung von rekombinanten eiweissen durch verwendung von hocheffizienten expressionsvektoren aus saccharomyces cerevisiae

Country Status (6)

Country Link
US (1) US6391585B1 (de)
EP (1) EP1015612B1 (de)
JP (1) JP3366339B2 (de)
AU (1) AU734761B2 (de)
DE (1) DE69732378D1 (de)
WO (1) WO1998054339A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10062302A1 (de) * 2000-12-14 2002-07-11 Aventis Res & Tech Gmbh & Co Sekretionssignalpeptide, deren DNA-Sequenzen, damit herstellbare Expressionsvektoren für eukaryotische Zellen und deren Verwendung zur biotechnologischen Herstellung von Proteinen
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
US7838287B2 (en) 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
CN1213062C (zh) * 2001-07-13 2005-08-03 一泰医药研究（深圳）有限公司 序列号13的生物活性肽
EP1947109A3 (de) 2001-07-13 2008-10-01 CMS Peptides Patent Holding Company Limited Biologisch aktive Peptide
US20060153813A1 (en) * 2003-07-06 2006-07-13 Quesenberry Peter J Methods of producing differentiated hematopoietic cells for treatment of cytopenia
MY140664A (en) * 2003-09-29 2010-01-15 Merck Sharp & Dohme Optimized expression of hpv 45 l1 in yeast
TWI353252B (en) 2004-04-28 2011-12-01 Cms Peptides Patent Holding Company Ltd Biologically active peptide vapeehptllteaplnpk der
US8080391B2 (en) * 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
EP1909815A4 (de) 2005-07-26 2011-05-18 Cms Peptides Patent Holding Company Ltd Neuartige biologisch aktive peptide und neue verwendungen dafür
WO2007026617A1 (ja) * 2005-08-29 2007-03-08 Asahi Glass Company, Limited 発現ベクター、それを導入した形質転換体、および異種タンパク質の製造方法
CA2708609A1 (en) * 2007-12-11 2009-06-18 Syngenta Participations Ag Engineering zymogen for conditional toxicity
EP2571991A1 (de) 2010-05-20 2013-03-27 Evolva SA Verfahren zur herstellung von isoprenoidverbindungen in hefe
CA2998579A1 (en) * 2015-10-19 2017-04-27 Sandoz Ag Method for producing a recombinant protein with reduced impurities

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0132309A3 (de) * 1983-06-27 1985-12-27 The Board Of Trustees Of The Leland Stanford Junior University Regulierbares eukaryotisches Promotorelement
DK203187A (da) * 1986-04-22 1987-10-23 Immunex Corp Human g-csf proteinekspression
US5013652A (en) * 1986-10-14 1991-05-07 Genex Corporation Composite yeast vectors
DE69033633T2 (de) * 1989-07-07 2001-05-03 Unilever Nv Verfahren zur Herstellung eines Proteins mittels eines durch Mehrfachkopie-Integrierung eines Expressionsvektors tranformierten Pilzes
FI92601C (fi) * 1992-03-11 1994-12-12 Marja Makarow Menetelmä hyötyproteiinien erittämiseksi hiivoista
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
EP0852625A2 (de) * 1995-09-25 1998-07-15 Chiron Corporation Sekretorische 'leader'-sequenz aus pichia zur protein-expression

Also Published As

Publication number Publication date
EP1015612A1 (de) 2000-07-05
EP1015612B1 (de) 2005-01-26
JP2001500388A (ja) 2001-01-16
US6391585B1 (en) 2002-05-21
AU734761B2 (en) 2001-06-21
AU3049297A (en) 1998-12-30
WO1998054339A1 (en) 1998-12-03
JP3366339B2 (ja) 2003-01-14

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