DE69716966T2 - Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung - Google Patents

Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung

Info

Publication number
DE69716966T2
DE69716966T2 DE69716966T DE69716966T DE69716966T2 DE 69716966 T2 DE69716966 T2 DE 69716966T2 DE 69716966 T DE69716966 T DE 69716966T DE 69716966 T DE69716966 T DE 69716966T DE 69716966 T2 DE69716966 T2 DE 69716966T2
Authority
DE
Germany
Prior art keywords
sequencing
dna molecules
direct
exponential amplification
application
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69716966T
Other languages
English (en)
Other versions
DE69716966D1 (de
Inventor
Prof Dr Paeaebo
Dr Kilger
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Priority to DE69716966T priority Critical patent/DE69716966T2/de
Publication of DE69716966D1 publication Critical patent/DE69716966D1/de
Application granted granted Critical
Publication of DE69716966T2 publication Critical patent/DE69716966T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69716966T 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung Expired - Fee Related DE69716966T2 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE69716966T DE69716966T2 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19653439A DE19653439A1 (de) 1996-12-20 1996-12-20 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
DE69716966T DE69716966T2 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung

Publications (2)

Publication Number Publication Date
DE69716966D1 DE69716966D1 (de) 2002-12-12
DE69716966T2 true DE69716966T2 (de) 2003-08-28

Family

ID=7815636

Family Applications (3)

Application Number Title Priority Date Filing Date
DE19653439A Ceased DE19653439A1 (de) 1996-12-20 1996-12-20 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
DE69713866T Expired - Fee Related DE69713866T2 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung
DE69716966T Expired - Fee Related DE69716966T2 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE19653439A Ceased DE19653439A1 (de) 1996-12-20 1996-12-20 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
DE69713866T Expired - Fee Related DE69713866T2 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen und seine Anwendung

Country Status (6)

Country Link
US (3) US6107032A (de)
EP (2) EP0849364B1 (de)
JP (1) JPH10295400A (de)
AT (2) ATE220426T1 (de)
DE (3) DE19653439A1 (de)
ES (1) ES2186293T3 (de)

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US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
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Also Published As

Publication number Publication date
EP0849364A1 (de) 1998-06-24
US6107032A (en) 2000-08-22
ATE220426T1 (de) 2002-07-15
US20040197811A1 (en) 2004-10-07
DE69713866D1 (de) 2002-08-14
DE69716966D1 (de) 2002-12-12
EP1004677B1 (de) 2002-11-06
US20020192661A1 (en) 2002-12-19
EP1004677A1 (de) 2000-05-31
DE19653439A1 (de) 1998-07-02
EP0849364B1 (de) 2002-07-10
DE69713866T2 (de) 2003-02-06
JPH10295400A (ja) 1998-11-10
ATE227347T1 (de) 2002-11-15
ES2186293T3 (es) 2003-05-01

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