DE69713059T2 - Tandem-fluoreszenzproteinkonstrukte - Google Patents

Tandem-fluoreszenzproteinkonstrukte

Info

Publication number
DE69713059T2
DE69713059T2 DE69713059T DE69713059T DE69713059T2 DE 69713059 T2 DE69713059 T2 DE 69713059T2 DE 69713059 T DE69713059 T DE 69713059T DE 69713059 T DE69713059 T DE 69713059T DE 69713059 T2 DE69713059 T2 DE 69713059T2
Authority
DE
Germany
Prior art keywords
fluorescent protein
donor
protein constructs
tandem fluorescent
acceptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69713059T
Other languages
English (en)
Other versions
DE69713059D1 (de
Inventor
Y Tsien
Roger Heim
Andrew Cubitt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vertex Pharmaceuticals San Diego LLC
University of California
Original Assignee
University of California
Aurora Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California, Aurora Biosciences Corp filed Critical University of California
Publication of DE69713059D1 publication Critical patent/DE69713059D1/de
Application granted granted Critical
Publication of DE69713059T2 publication Critical patent/DE69713059T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE69713059T 1996-01-31 1997-01-31 Tandem-fluoreszenzproteinkonstrukte Expired - Lifetime DE69713059T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/594,575 US6803188B1 (en) 1996-01-31 1996-01-31 Tandem fluorescent protein constructs
PCT/US1997/001457 WO1997028261A1 (en) 1996-01-31 1997-01-31 Tandem fluorescent protein constructs

Publications (2)

Publication Number Publication Date
DE69713059D1 DE69713059D1 (de) 2002-07-11
DE69713059T2 true DE69713059T2 (de) 2002-12-19

Family

ID=24379467

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69713059T Expired - Lifetime DE69713059T2 (de) 1996-01-31 1997-01-31 Tandem-fluoreszenzproteinkonstrukte

Country Status (10)

Country Link
US (4) US6803188B1 (de)
EP (1) EP0877805B1 (de)
AT (1) ATE218615T1 (de)
AU (1) AU717873B2 (de)
CA (1) CA2245108C (de)
DE (1) DE69713059T2 (de)
DK (1) DK0877805T3 (de)
ES (1) ES2177939T3 (de)
PT (1) PT877805E (de)
WO (1) WO1997028261A1 (de)

Families Citing this family (160)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5625048A (en) 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6900304B2 (en) 1996-01-31 2005-05-31 The Regents Of The University Of California Emission ratiometric indicators of phosphorylation
US6803188B1 (en) 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US7388092B2 (en) * 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7825237B2 (en) * 1996-05-03 2010-11-02 Applied Biosystems, Llc Oligonucleotides and analogs labeled with energy transfer dyes
US6027881A (en) * 1996-05-08 2000-02-22 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Mutant Aequorea victoria fluorescent proteins having increased cellular fluorescence
US5912137A (en) * 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US5925558A (en) * 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US6124128A (en) * 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US6593135B2 (en) 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US6756207B1 (en) 1997-02-27 2004-06-29 Cellomics, Inc. System for cell-based screening
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
JP2002506521A (ja) * 1997-06-05 2002-02-26 カイロス サイエンティフィック インコーポレーテッド マイクロスコピーにおける蛍光共鳴エネルギー伝達の較正
FR2764387B1 (fr) * 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
DE19737562A1 (de) * 1997-08-28 1999-05-06 Otogene Biotechnologische Fors Verfahren zur Identifizierung von Wechselwirkungen zwischen Proteinen bzw. Peptiden
US7105307B2 (en) 1997-08-30 2006-09-12 Cyclacel, Ltd. Compositions and methods for screening for modulators of enzymatic activity
DE19756773A1 (de) 1997-12-19 1999-06-24 Dade Behring Marburg Gmbh Neues Verfahren und Diagnosemittel zur Hämostase-Diagnostik
US6884870B2 (en) * 1998-03-20 2005-04-26 California Institute Of Technology Fusion proteins for identifying proteases, protease target sites and regulators of protease activity in living cells
EP1925320A3 (de) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferasen, fluoreszierende Proteine, Nukleinsäuren zur Kodierung der Luciferasen und der fluoreszierenden Proteine sowie deren Verwendung zur Diagnose, zum Screening mit hohem Durchsatz und zur Behandlung neuartiger Probleme
DE69938293T2 (de) * 1998-03-27 2009-03-12 Bruce J. Beverly Hills Bryan Luciferase, gfp fluoreszenzproteine, kodierende nukleinsaüre und ihre verwendung in der diagnose
US6897031B1 (en) * 1998-04-17 2005-05-24 Rigel Pharmaceuticals, Inc. Multiparameter FACS assays to detect alterations in exocytosis
DE19829495A1 (de) * 1998-07-02 2000-01-05 Jacques Paysan Reagenzien und deren Anwendung zur Untersuchung von Wechselwirkungen zwischen zellulären Molekülen und deren Lokalisation in Zellen
US6495664B1 (en) 1998-07-24 2002-12-17 Aurora Biosciences Corporation Fluorescent protein sensors of post-translational modifications
JP2002522040A (ja) * 1998-08-08 2002-07-23 インペリアル キャンサー リサーチ テクノロジー リミテッド 改変されたグリーン蛍光タンパク質
US9834584B2 (en) * 1998-12-11 2017-12-05 Takara Bio Usa, Inc. Nucleic acids encoding chromophores/fluorophores and methods for using the same
WO2001027150A2 (en) 1999-10-14 2001-04-19 Clontech Laboratories Inc. Anthozoa derived chromo/fluoroproteins and methods for using the same
GB9904407D0 (en) * 1999-02-25 1999-04-21 Fluorescience Ltd Compositions and methods for monitoring the modification of engineered binding partners
US6670144B1 (en) 1999-02-26 2003-12-30 Cyclacel, Ltd. Compositions and methods for monitoring the phosphorylation of natural binding partners
US6972182B1 (en) 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using coiled binding partners
US7166475B2 (en) * 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
US6656696B2 (en) 1999-02-26 2003-12-02 Cyclacel Compositions and methods for monitoring the phosphorylation of natural binding partners
US6828106B2 (en) * 1999-02-26 2004-12-07 Cyclacel Limited Methods and compositions using coiled binding partners
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
WO2000073437A1 (en) * 1999-05-27 2000-12-07 Merck Frosst Canada & Co. Assays for caspase activity using green fluorescent proteins
US20020177120A1 (en) * 1999-06-04 2002-11-28 Kathryn J. Elliott Assays for apotosis modulators
US6358684B1 (en) * 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
DE19941039A1 (de) * 1999-08-28 2001-03-01 Boehringer Ingelheim Pharma gamma-Sekretase in vitro Testsystem
GB9927331D0 (en) * 1999-11-18 2000-01-12 Fluorescience Ltd Assay for measuring enzyme activity in vivo
US7060506B2 (en) 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
US7262005B1 (en) 2000-02-04 2007-08-28 Aurora Biosciences Corporation Methods of protein destabilization and uses thereof
US20020076741A1 (en) * 2000-02-11 2002-06-20 Tencza Sarah Burroughs Peptide biosensors for anthrax protease
US7029868B2 (en) * 2000-02-17 2006-04-18 Merck & Co., Inc. Cell fusion assays using fluorescence resonance energy transfer
WO2001062919A1 (en) * 2000-02-23 2001-08-30 Aurora Biosciences Corporation Modified fluorescent proteins
WO2002010433A2 (en) * 2000-05-03 2002-02-07 Expressive Constructs, Inc. Method and device for detecting bacterial contamination
US20030096315A1 (en) 2000-05-03 2003-05-22 Sanders Mitchell C. Device for detecting bacterial contamination and method of use
US6808874B2 (en) 2000-06-13 2004-10-26 Cyclacel Ltd. Methods of monitoring enzyme activity
JP2004504018A (ja) * 2000-07-19 2004-02-12 ファルマシア・アンド・アップジョン・カンパニー β−セレクターゼ活性の基質およびアッセイ
AU2001232219A1 (en) * 2000-08-14 2001-06-06 Michiyuki Matsuda Protein monitoring the activity of small gtp-binding protein
CA2419503A1 (en) * 2000-08-14 2003-02-14 Michiyuki Matsuda Protein monitoring the activity of low-molecular weight gtp-binding protein
DE10041238A1 (de) * 2000-08-22 2002-03-07 Aventis Res & Tech Gmbh & Co Verfahren zur Identifizierung spezifisch spaltbarer peptide und Verwendung solcher Peptidsequenzen
GB0024351D0 (en) * 2000-10-04 2000-11-22 Amersham Pharm Biotech Uk Ltd Dye-labelled peptide and method
US7390458B2 (en) * 2000-10-13 2008-06-24 Irm Llc High throughput processing system and method of using
US7378280B2 (en) 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
AU2002230920A1 (en) * 2000-12-15 2002-06-24 Stratagene California Dimeric fluorescent polypeptides
WO2002063035A2 (en) * 2001-02-08 2002-08-15 The Penn State Research Foundation Fluorescent assay for proteolysis
GB0103763D0 (en) * 2001-02-15 2001-04-04 Glaxo Group Ltd Pak-Gep Construct
US7687614B2 (en) * 2001-02-26 2010-03-30 The Regents Of The University Of California Monomeric and dimeric fluorescent protein variants and methods for making same
US7005511B2 (en) 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
JP4215512B2 (ja) * 2001-02-26 2009-01-28 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 非オリゴマー化タンデム蛍光性タンパク質
AU2002254173A1 (en) * 2001-03-12 2002-10-08 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The Unive Oxidation-reduction sensitive green fluorescent protein variants
GB0111459D0 (en) * 2001-05-10 2001-07-04 Isis Innovation Universal fluorescent sensors
US7354735B2 (en) * 2001-05-24 2008-04-08 Hong Kong University Of Science And Technology Nucleic acid encoding fluorescent protein constructs and methods for detecting apoptosis
JP3829252B2 (ja) * 2001-06-08 2006-10-04 独立行政法人理化学研究所 蛍光蛋白質
US20030100031A1 (en) * 2001-06-11 2003-05-29 Steven Dower Integrative assays for monitoring molecular assembly events
US6780974B2 (en) * 2001-06-12 2004-08-24 Cellomics, Inc. Synthetic DNA encoding an orange seapen-derived green fluorescent protein with codon preference of mammalian expression systems and biosensors
IL160519A0 (en) * 2001-08-23 2004-07-25 Qtl Biosystems Llc Bio-sensing platforms for detection and quantitation of biological molecules
US7332567B2 (en) * 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US7374896B2 (en) * 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US7208285B2 (en) * 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US20030108972A1 (en) * 2001-12-06 2003-06-12 Zweig Stephen Eliot Tethered receptor-ligand reagent and assay
GB0126976D0 (en) * 2001-11-09 2002-01-02 Microsens Biophage Ltd Multiple virus reaction assay
ES2329666T3 (es) * 2001-12-19 2009-11-30 The University Of Chicago Proteinas fluorescentes de maduracion rapida y procedimientos de uso de las mismas.
US20030166037A1 (en) * 2002-01-16 2003-09-04 Fourie Anne M. Aggrecanase-1 and -2 peptide substrates and methods
EP1468116A2 (de) 2002-01-16 2004-10-20 Dynal Biotech ASA Verfahren zur isolierung von nukleinsäuren und proteinen aus einer einzigen probe
AU2003212897B2 (en) 2002-01-31 2007-08-02 Expressive Constructs, Inc. Method for detecting microorganisms
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
WO2003087152A1 (en) * 2002-04-11 2003-10-23 Mount Sinai Hospital Luminescent markers
GB0208987D0 (en) * 2002-04-19 2002-05-29 Amersham Biosciences Uk Ltd Methods for measuring protein kinase and phosphatase activity
US6916660B2 (en) * 2002-05-14 2005-07-12 North Carolina State University Fluorescent sensor compounds for detecting saccharides
US6942987B2 (en) * 2002-05-15 2005-09-13 Pharmacopeia Drug Discovery, Inc. Methods for measuring kinase activity
US20080020405A1 (en) * 2002-07-17 2008-01-24 Mynarcik Dennis C Protein binding determination and manipulation
US20040042961A1 (en) * 2002-07-31 2004-03-04 Robert Menard Development of an in vivo functional assay for proteases
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
US7951923B2 (en) 2002-11-12 2011-05-31 Zakrytoe Aktsionernoe Obschestvo ‘Evrogen’ Fluorescent proteins and chromoproteins from non-Aequorea hydrozoa species and methods for using same
US7056683B2 (en) * 2002-11-12 2006-06-06 Massachusetts Institute Of Technology Genetically encoded fluorescent reporters of kinase, methyltransferase, and acetyl-transferase activities
CA2505721A1 (en) * 2002-11-18 2004-06-03 Genospectra, Inc. Rnai-based sensors, caged interfering rnas, and methods of use thereof
US7413874B2 (en) 2002-12-09 2008-08-19 University Of Miami Nucleic acid encoding fluorescent proteins from aquatic species
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
WO2004058973A1 (en) 2002-12-26 2004-07-15 Zakrytoe Aktsionernoe Obschestvo 'evrogen' Fluorescent proteins from copepoda species and methods for using same
US20050221443A1 (en) * 2003-01-06 2005-10-06 Xencor, Inc. Tumor necrosis factor super family agonists
US20050130892A1 (en) * 2003-03-07 2005-06-16 Xencor, Inc. BAFF variants and methods thereof
US20060014248A1 (en) * 2003-01-06 2006-01-19 Xencor, Inc. TNF super family members with altered immunogenicity
US7553930B2 (en) * 2003-01-06 2009-06-30 Xencor, Inc. BAFF variants and methods thereof
AU2004204942A1 (en) * 2003-01-08 2004-07-29 Xencor, Inc Novel proteins with altered immunogenicity
US7759459B2 (en) * 2003-01-10 2010-07-20 Albert Einstein College Of Medicine Of Yeshiva University Fluorescent assays for protein kinases
WO2005012556A2 (en) 2003-01-31 2005-02-10 Ethicon, Inc. Cationic anti-microbial peptides and methods of use thereof
DE10328775B4 (de) * 2003-06-25 2007-01-04 Ruprecht-Karls-Universität Heidelberg In vivo-Testsystem zum Nachweis von HIV-Proteaseaktivität
US7803559B1 (en) 2003-08-08 2010-09-28 The Regents Of The University Of Ca Protein aggregation regulators
US20050037364A1 (en) 2003-08-13 2005-02-17 International Business Machines Corporation Techniques for recording signals
CA2542353A1 (en) 2003-10-10 2005-04-21 Xencor, Inc. Protein based tnf-alpha variants for the treatment of tnf-alpha related disorders
AU2004286342B2 (en) 2003-11-03 2008-10-02 Systagenix Wound Management Ip Co. B.V. Methods, peptides and biosensors useful for detecting a broad spectrum of bacteria
US20050214890A1 (en) * 2003-11-26 2005-09-29 Zhiqun Tan Novel "Cleave-N-Read" system for protease activity assay and methods of use thereof
US8137924B2 (en) 2003-12-19 2012-03-20 Wisconsin Alumni Research Foundation Method and compositions for detecting botulinum neurotoxin
AU2005216013B2 (en) 2004-01-30 2010-11-11 Dana Farber Cancer Institute Method for determination and quantification of radiation or genotoxin exposure
US7250298B2 (en) * 2004-04-07 2007-07-31 The University Of Chicago Monomeric red fluorescent proteins
US8236523B2 (en) * 2004-08-23 2012-08-07 The Johns Hopkins University Camp reporters and high throughput assays
US7399607B2 (en) * 2004-09-22 2008-07-15 Allergan, Inc. Fluorescence polarization assays for determining clostridial toxin activity
ATE540048T1 (de) 2004-10-14 2012-01-15 Carnegie Inst Of Washington Neurotransmittersensoren und verfahren zu ihrer verwendung
CA2584108A1 (en) 2004-10-14 2006-04-27 Carnegie Institution Of Washington Development of sensitive fret sensors and methods of using the same
WO2006060739A2 (en) * 2004-12-01 2006-06-08 Proteologics, Inc. Ubiquitin ligase assays and related reagents
US20090178149A1 (en) * 2005-03-03 2009-07-09 Sylvie Lalonde Polyamine Sensors and Methods of Using the Same
JP5161588B2 (ja) * 2005-03-04 2013-03-13 カーネギー インスチチューション オブ ワシントン 環境的に安定なセンサおよびそれを使用する方法
US20090149338A1 (en) * 2005-09-30 2009-06-11 Hughes Thomas E System for detecting protein-protein interactions
DE602006012072D1 (de) * 2005-10-12 2010-03-18 Allergan Inc Tests der molekularen oder subzellulären interaktivität unter verwendung von depolarisierung nach resonanzenergietransfer (daret)
US8173863B2 (en) 2005-10-14 2012-05-08 Carnegie Institution Of Washington Sucrose biosensors and methods of using the same
DE602005023668D1 (de) * 2005-10-14 2010-10-28 Carnegie Inst Of Washington Phosphat-biosensoren und verfahren zu deren anwendung
RU2412250C2 (ru) * 2005-11-04 2011-02-20 Закрытое акционерное общество "ЕВРОГЕН" Модифицированные зеленые флуоресцентные белки и способы их использования
US20080311047A1 (en) * 2005-11-16 2008-12-18 Thijs Kaper Multimetric Biosensors and Methods of Using Same
CA2641421C (en) 2005-12-30 2014-07-29 Arbor Vita Corporation Small molecule inhibitors of pdz interactions
DK1994149T3 (da) 2006-01-25 2011-01-17 Evrogen Ip Nye fluorescerende proteiner og fremgangsmåder til anvendelse deraf
US8563703B2 (en) 2006-01-25 2013-10-22 Evrogen IP Joint Stock Company Fluorescent proteins and methods for using same
US7858386B2 (en) * 2006-03-07 2010-12-28 The United States Of America As Represented By The Secretary Of The Navy Method of controlling quantum dot photoluminescence and other intrinsic properties through biological specificity
US8680235B2 (en) * 2006-09-22 2014-03-25 Stowers Institute For Medical Research Branchiostoma derived fluorescent proteins
US20100196355A1 (en) * 2007-01-29 2010-08-05 Wyeth Immunophilin Ligands and Methods for Modulating Immunophilin and Calcium Channel Activity
US8962677B2 (en) * 2007-07-12 2015-02-24 Acumen Pharmaceuticals, Inc. Methods of restoring cognitive ability using non-peptidic compounds
US9006283B2 (en) 2007-07-12 2015-04-14 Acumen Pharmaceuticals, Inc. Methods of modifying amyloid β oligomers using non-peptidic compounds
US20110098309A1 (en) * 2007-07-12 2011-04-28 Acumen Pharmaceuticals, Inc. Methods of inhibiting the formation of amyloid-beta diffusable ligands using acylhydrazide compounds
CN101932936B (zh) * 2007-09-14 2016-04-27 拜奥麦迪逊公司 利用切割序列和间隔子的共振能量转移测定
US8679749B2 (en) * 2007-11-01 2014-03-25 The University Of Chicago Red fluorescent proteins with enhanced bacterial expression, increased brightness and reduced aggregation
WO2009079566A2 (en) 2007-12-18 2009-06-25 Acumen Pharmaceuticals, Inc. Novel addl receptor polypeptides, polynucleotides and host cells for recombinant production
WO2010038228A2 (en) * 2008-10-02 2010-04-08 Ramot At Tel Aviv University Ltd. Method and system for emitting light
DE102008043724A1 (de) * 2008-11-13 2010-05-20 Biotronik Vi Patent Ag Erhöhung der Effizienz pharmazeutische Wirkstoffe-freisetzender Medizinprodukte durch Kombination mit einem Inhibitor des Transportproteins P-Glycoprotein
US8962341B2 (en) 2008-12-12 2015-02-24 The Commonwealth Medical College Cell-based detection of APF through its interaction with CKAP4 for diagnosis of interstitial cystitis
WO2011042874A1 (en) * 2009-10-06 2011-04-14 Ecole Polytechnique Federale De Lausanne (Epfl) Visualization of proprotein convertase activity in living cells and tissues
US10246492B2 (en) 2009-10-16 2019-04-02 Biomadison, Inc. Botulinum assay with synaptobrevin substrate moiety
GB201121565D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Engineering fluorescent proteins for enhanced fret and uses thereof
GB201121570D0 (en) 2011-12-15 2012-01-25 Fundaci Privada Ct De Regulaci Gen Mica Crg Methods for producing engineered fluorescent proteins for enhanced fret, products and uses thereof
WO2013142859A2 (en) 2012-03-23 2013-09-26 Wuhan Peptech Pharmaceutical Co., Ltd. Fusion proteins of superfolder green fluorescent protein and use thereof
ES2445018B1 (es) * 2012-07-27 2014-12-12 Universidad De Castilla La Mancha VARIANTES DE LA PROTEÍNA DE FUSIÓN SENSIBLE AL CALCIO tdTOMATO-AEQUORINA
KR101974583B1 (ko) * 2012-08-29 2019-05-02 삼성전자주식회사 링커 폴리펩티드 및 이를 이용한 표적 물질 분석 방법
GB201217770D0 (en) * 2012-10-04 2012-11-14 Base4 Innovation Ltd Biological probes and the use thereof
CN104280366B (zh) * 2013-07-03 2017-10-10 复旦大学 一种高通量检测化合物的靶标蛋白的方法
US10670607B2 (en) * 2014-02-27 2020-06-02 University Of Helsinki Protein L based bioassay method for determining presence of soluble antibodies in a sample and kit therefor
JP6473366B2 (ja) * 2015-03-31 2019-02-20 シスメックス株式会社 温度判定方法、標的ペプチドの検出方法および温度判定試薬
ITUB20155272A1 (it) 2015-11-02 2017-05-02 Scuola Normale Superiore Intracellular antibody
WO2017127759A1 (en) * 2016-01-22 2017-07-27 The Regents Of The University Of California Formation and use of embedded solutions in nanoscale materials
US20210061843A1 (en) * 2018-01-22 2021-03-04 Modernatx, Inc. Multimeric polynucleotides and uses thereof
US11834698B2 (en) 2018-05-02 2023-12-05 Indian Institute Of Science Education And Research Process for determining enzyme activity in a cell by activity-based reporter gene technology (ABRGT)
CN116063546A (zh) * 2021-10-29 2023-05-05 深圳先进技术研究院 一种基于共振能量转移的全遗传编码nad+蛋白探针及其制备方法和应用

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL60888A (en) 1978-06-16 1984-07-31 Yeda Res & Dev Substrates and process for the quantitative assay of enzymes
GB8916806D0 (en) 1989-07-22 1989-09-06 Univ Wales Medicine Modified proteins
EP0428000A1 (de) 1989-11-03 1991-05-22 Abbott Laboratories Fluorogene Substrate zum Nachweis von proteolytischer Enzymaktivität
US5599906A (en) 1990-04-13 1997-02-04 Schering Corporation Protease assays
US5264563A (en) 1990-08-24 1993-11-23 Ixsys Inc. Process for synthesizing oligonucleotides with random codons
US5491074A (en) 1993-04-01 1996-02-13 Affymax Technologies Nv Association peptides
GB9310978D0 (en) 1993-05-27 1993-07-14 Zeneca Ltd Compounds
KR960705209A (ko) 1993-09-10 1996-10-09 잭 엠. 그랜노위츠 녹색 형광 단백의 용도
US5491084A (en) 1993-09-10 1996-02-13 The Trustees Of Columbia University In The City Of New York Uses of green-fluorescent protein
WO1995021191A1 (en) 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US5605809A (en) 1994-10-28 1997-02-25 Oncoimmunin, Inc. Compositions for the detection of proteases in biological samples and methods of use thereof
US5625048A (en) * 1994-11-10 1997-04-29 The Regents Of The University Of California Modified green fluorescent proteins
US5777079A (en) * 1994-11-10 1998-07-07 The Regents Of The University Of California Modified green fluorescent proteins
US5602021A (en) 1994-12-29 1997-02-11 Catalytic Antibodies, Inc. Method for generating proteolytic enzymes specific against a selected peptide sequence
ES2170220T3 (es) 1995-01-31 2002-08-01 Bioimage As Procedimiento para detectar sustancias biologicamente activas.
WO1996027027A1 (en) 1995-03-02 1996-09-06 Rutgers The State University Of New Jersey Improved method for purifying green fluorescent protein
GB9504446D0 (en) 1995-03-06 1995-04-26 Medical Res Council Improvements in or relating to gene expression
US5614191A (en) * 1995-03-15 1997-03-25 The United States Of America As Represented By The Department Of Health And Human Services IL-13 receptor specific chimeric proteins and uses thereof
ATE184613T1 (de) 1995-09-22 1999-10-15 Novo Nordisk As Varianten des grünen fluoreszenzproteins, gfp
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US6593135B2 (en) * 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
US6124128A (en) * 1996-08-16 2000-09-26 The Regents Of The University Of California Long wavelength engineered fluorescent proteins
US6197928B1 (en) * 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US5998204A (en) * 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6046925A (en) * 1997-04-14 2000-04-04 The Regents Of The University Of California Photochromic fluorescent proteins and optical memory storage devices based on fluorescent proteins
US6140132A (en) * 1998-06-09 2000-10-31 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6150176A (en) * 1998-06-09 2000-11-21 The Regents Of The University Of California Fluorescent protein sensors for measuring the pH of a biological sample
US6469154B1 (en) * 1999-05-21 2002-10-22 The Regents Of The University Of California Fluorescent protein indicators

Also Published As

Publication number Publication date
US20100233726A1 (en) 2010-09-16
DK0877805T3 (da) 2002-08-26
PT877805E (pt) 2002-10-31
US6803188B1 (en) 2004-10-12
CA2245108C (en) 2003-04-01
US5981200A (en) 1999-11-09
AU717873B2 (en) 2000-04-06
DE69713059D1 (de) 2002-07-11
ATE218615T1 (de) 2002-06-15
WO1997028261A1 (en) 1997-08-07
CA2245108A1 (en) 1997-08-07
US20020164674A1 (en) 2002-11-07
EP0877805A1 (de) 1998-11-18
ES2177939T3 (es) 2002-12-16
EP0877805B1 (de) 2002-06-05
US8679742B2 (en) 2014-03-25
AU2250497A (en) 1997-08-22

Similar Documents

Publication Publication Date Title
ATE218615T1 (de) Tandem-fluoreszenzproteinkonstrukte
BR9803827B1 (pt) máquina elétrica hìbrida com excitação dupla.
CY1111006T1 (el) Εκριμματαση ασθενειας alzheimer
GB9811483D0 (en) Luminescence assay using cyclical excitation wavelength sequence
DK1137812T3 (da) DNA-proteinfusioner og anvendelser deraf
DE69939820D1 (de) Internalisierende erbb2 antikörper
DE59506355D1 (de) Chromatographische isolierung von nucleinsäuren
ATE284407T1 (de) Pyrimidinderivate als markierte bindungspartner
WO2000009677A3 (en) Sterol-regulated site-1 protease and assays of modulators thereof
ATE439441T1 (de) Veränderte, fluoreszierende proteine
ATE285474T1 (de) Haemophilus adhäsionsproteinen
DE69739346D1 (de) Gereinigtes sr-p70 protein
CY1106384T1 (el) Δοκιμασιες τροποποιητη υποδοχεα ενepγοποιουμενου απο πρωτεαση (par)
EA200000567A1 (ru) Бактериальные плазмиды
AU9437598A (en) Method and kit for identifying interactions between proteins or peptides
ATE321768T1 (de) Linker-nucleosid, seine herstellung und verwendung
ES2179908T3 (es) Acidos sacarosamonocarboxilicos acilados.
AUPO470197A0 (en) Fluorescent dyes from microorganisms
ES1040213Y (es) Mondadientes triangular plano.
IT1296071B1 (it) Driver con accoppiamento in alternata, con dinamica di uscita elevata
NL1004200A1 (nl) Hulpstukkoppeling.
ES1037643Y (es) Papelera ecologica multiple.
ES1030372Y (es) Encendedor solar ecologico.
KR970045642U (ko) 피.엘.씨 출력카드

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: VERTEX PHARMACEUTICALS(SAN DIEGO)LLC(N.D.GES.D.STA

Owner name: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, OAKLA