DE69636553D1 - Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung - Google Patents

Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung

Info

Publication number
DE69636553D1
DE69636553D1 DE69636553T DE69636553T DE69636553D1 DE 69636553 D1 DE69636553 D1 DE 69636553D1 DE 69636553 T DE69636553 T DE 69636553T DE 69636553 T DE69636553 T DE 69636553T DE 69636553 D1 DE69636553 D1 DE 69636553D1
Authority
DE
Germany
Prior art keywords
nucleic acid
magnetic carrier
binding magnetic
isolation
acid isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Revoked
Application number
DE69636553T
Other languages
English (en)
Other versions
DE69636553T2 (de
Inventor
Hiroaki Uematsu
Katsuya Daimon
Satoko Yoshiga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Toyobo Co Ltd
Original Assignee
Toyobo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37068234&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69636553(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from JP7172481A external-priority patent/JP2965131B2/ja
Application filed by Toyobo Co Ltd filed Critical Toyobo Co Ltd
Application granted granted Critical
Publication of DE69636553D1 publication Critical patent/DE69636553D1/de
Publication of DE69636553T2 publication Critical patent/DE69636553T2/de
Anticipated expiration legal-status Critical
Revoked legal-status Critical Current

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B03SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03CMAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
    • B03C1/00Magnetic separation
    • B03C1/005Pretreatment specially adapted for magnetic separation
    • B03C1/01Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y25/00Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326Magnetic particles
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F1/00Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
    • H01F1/0036Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
    • H01F1/0045Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
    • H01F1/0063Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use in a non-magnetic matrix, e.g. granular solids
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F1/00Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
    • H01F1/01Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials
    • H01F1/03Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity
    • H01F1/032Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials
    • H01F1/10Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites, e.g. [(Ba,Sr)O(Fe2O3)6] ferrites with hexagonal structure
    • H01F1/11Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites, e.g. [(Ba,Sr)O(Fe2O3)6] ferrites with hexagonal structure in the form of particles
    • H01F1/111Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites, e.g. [(Ba,Sr)O(Fe2O3)6] ferrites with hexagonal structure in the form of particles with a non-magnetic core
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01FMAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
    • H01F1/00Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
    • H01F1/0036Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
    • H01F1/0045Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
    • H01F1/0054Coated nanoparticles, e.g. nanoparticles coated with organic surfactant

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Power Engineering (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DE69636553T 1995-07-07 1996-07-05 Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung Revoked DE69636553T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP17248195 1995-07-07
JP7172481A JP2965131B2 (ja) 1995-07-07 1995-07-07 核酸結合用磁性担体およびそれを用いる核酸単離方法

Publications (2)

Publication Number Publication Date
DE69636553D1 true DE69636553D1 (de) 2006-10-26
DE69636553T2 DE69636553T2 (de) 2007-05-31

Family

ID=37068234

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69636553T Revoked DE69636553T2 (de) 1995-07-07 1996-07-05 Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung

Country Status (2)

Country Link
EP (1) EP1260595B1 (de)
DE (1) DE69636553T2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112007605A (zh) * 2019-05-30 2020-12-01 苏州海狸生物医学工程有限公司 一种用于核酸提取的羟基纳米磁珠及其制备方法

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7524630B2 (en) 2002-04-22 2009-04-28 University Of Florida Research Foundation, Inc. Functionalized nanoparticles and methods of use
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US8409463B1 (en) 2007-07-16 2013-04-02 University Of Central Florida Research Foundation, Inc. Aqueous method for making magnetic iron oxide nanoparticles
US9057094B1 (en) 2007-10-25 2015-06-16 University Of Central Florida Research Foundation, Inc. Nanoparticle-mediated methods for antimicrobial susceptibility testing of bacteria
EP2345719A1 (de) 2010-01-18 2011-07-20 Qiagen GmbH Verfahren zur Isolierung kleiner RNA
EP2407540A1 (de) 2010-07-15 2012-01-18 Qiagen GmbH Verfahren zur Reinigung von Nukleinsäure
US9109249B2 (en) 2011-05-19 2015-08-18 University Of Central Florida Research Foundation, Inc. Microbe detection via hybridizing magnetic relaxation nanosensors
WO2013024072A1 (en) 2011-08-12 2013-02-21 Qiagen Gmbh Method for isolating nucleic acids
EP2634254A1 (de) 2012-02-29 2013-09-04 QIAGEN GmbH Verfahren zur Isolierung von Nukleinsäuren aus Nahrungsmittelproben
DE102012012523B4 (de) * 2012-06-26 2015-02-12 Magnamedics Gmbh Reinigung von Nukleinsäuren
US20150225712A1 (en) 2012-08-21 2015-08-13 Qiagen Gmbh Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
EP2888354B1 (de) 2012-08-21 2020-04-01 Qiagen GmbH Stabilisierung von viruspartikeln und viralen nukleinsäuren in einer biologischen probe
AU2013310861B2 (en) 2012-09-03 2019-02-14 Qiagen Gmbh Method for isolating RNA including small RNA with high yield
US10745686B2 (en) 2013-02-08 2020-08-18 Qiagen Gmbh Method for separating DNA by size
JP2015073485A (ja) * 2013-10-09 2015-04-20 セイコーエプソン株式会社 核酸増幅方法、核酸抽出用デバイス、核酸増幅反応用カートリッジ、及び核酸増幅反応用キット
EP3059312A1 (de) 2015-02-20 2016-08-24 QIAGEN GmbH Nukleinsäureextraktionsverfahren
US10794859B2 (en) 2015-06-01 2020-10-06 Qiagen Gmbh Electrophoresis assisted method and device for purifying a charged target molecule from a sample
EP3303580B1 (de) 2015-06-01 2020-11-18 Qiagen GmbH Elektrophoreseunterstütztes verfahren zur reinigung von ziel-nukleinsäure mittels ansatz der verzögerten elution
WO2016193490A1 (en) 2015-06-05 2016-12-08 Qiagen Gmbh Method for separating dna by size
CN105214596B (zh) * 2015-09-23 2017-10-31 郑州大学 一种用于动物组织核酸提取的磁性微球
CN105112404B (zh) * 2015-09-23 2018-02-27 河南中标检测服务有限公司 一种动物组织核酸提取液的制备方法
US20210087606A1 (en) * 2017-07-14 2021-03-25 Capitalbio Corporation Solid phase extraction material and its use for nucleic acid enrichment and detection
CN111148834B (zh) 2017-09-27 2024-06-11 凯杰有限公司 以高收率分离rna的方法
AU2019306640B2 (en) * 2018-07-19 2022-09-22 Beckman Coulter, Inc. Magnetic particles
CN112604645A (zh) * 2020-12-10 2021-04-06 广东省测试分析研究所(中国广州分析测试中心) 一种纳米磁粒及其制备方法和应用
CN117126842A (zh) * 2022-05-27 2023-11-28 杭州诺辉健康科技有限公司 利用多孔纳米材料进行核酸提取和纯化的方法和试剂

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
WO1989004373A1 (en) * 1987-10-26 1989-05-18 Baxter International Inc. Process for producing magnetically responsive polymer particles and application thereof
EP0343934B1 (de) * 1988-05-24 1995-01-25 Anagen (U.K.) Limited Magnetisch anziehbare Teilchen und Herstellungsverfahren
WO1990006045A2 (en) * 1988-11-21 1990-06-14 Dynal As Nucleic acid probes
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
AU8951191A (en) * 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5610274A (en) * 1991-11-20 1997-03-11 Cpg, Inc. Production and use of magnetic porous inorganic materials
GB9223334D0 (en) * 1992-11-06 1992-12-23 Hybaid Ltd Magnetic solid phase supports
AU7375794A (en) * 1993-07-28 1995-02-28 Akzo Nobel N.V. Process for isolating nucleic acid from gram positive microorganisms
EP0723549B1 (de) * 1993-08-30 2003-12-17 Promega Corporation Zusammensetzungen und verfahren zur reinigung von nukleinsäuren

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112007605A (zh) * 2019-05-30 2020-12-01 苏州海狸生物医学工程有限公司 一种用于核酸提取的羟基纳米磁珠及其制备方法

Also Published As

Publication number Publication date
EP1260595B1 (de) 2006-09-13
EP1260595A3 (de) 2002-12-18
EP1260595A2 (de) 2002-11-27
DE69636553T2 (de) 2007-05-31

Similar Documents

Publication Publication Date Title
DE69636553D1 (de) Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung
EP0757106A3 (de) Nukleinsäuren magnetischer Binder und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung
DE69619864T2 (de) Verfahren zur amplifizierung spezifischer nukleinsäuresequenzen
DE69519809D1 (de) Bandulierungssystem und verfahren zu dessen verwendung
HUP9602289A2 (en) Method for amplifying nucleic acid
DE59609524D1 (de) Verfahren zur Genexpressionsanalyse
DE69630732D1 (de) Mehrzelliges Dämpfungselement und Verfahren zur dessen Herstellung
DE59307620D1 (de) Testträger zur Analytbestimmung sowie Verfahren zu dessen Herstellung
DE59511093D1 (de) Verfahren zum direkten Nachweisen weniger Nukleinsäurestränge
DE69534886D1 (de) Verfahren zur Isolierung von Zellkomponenten
DE59900006D1 (de) Verfahren zur Isolierung von gekrümmten Flächen
DE69531765D1 (de) Verfahren zur heimatkanaltrennnung
ATE212380T1 (de) Verfahren zur herstellung von nikotinsäureamid
DE59610517D1 (de) Befestigungselement und Verfahren zu dessen Herstellung
DE69920967D1 (de) Verfahren zur cvd unter verwendung von bi-alkoxiden
DE69501893D1 (de) Verfahren zur genetischen prüfung
DE69601540D1 (de) Verfahren zur oxychlorierung
DE69823174D1 (de) Verfahren zur cvd bei niedrigen temperaturen unter verwendung von bi-carboxylaten
DE59601365D1 (de) Verfahren zum Isolieren von Formspulen
DE69605561D1 (de) Verfahren zur beschichtung
DE59301973D1 (de) Pipettierhilfe und verfahren zu ihrer herstellung
DE69728226D1 (de) Träger für Bioreaktor sowie Verfahren zu dessen Herstellung
DE69637516D1 (de) Verfahren für die Amplifizierung von Nukleinsäure-Sequenzen
DE69609963T2 (de) Verfahren zur iodierung
DE69840011D1 (de) e und Verfahren zu dessen Herstellung

Legal Events

Date Code Title Description
8363 Opposition against the patent
8331 Complete revocation