DE69636553D1 - Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung - Google Patents
Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen VerwendungInfo
- Publication number
- DE69636553D1 DE69636553D1 DE69636553T DE69636553T DE69636553D1 DE 69636553 D1 DE69636553 D1 DE 69636553D1 DE 69636553 T DE69636553 T DE 69636553T DE 69636553 T DE69636553 T DE 69636553T DE 69636553 D1 DE69636553 D1 DE 69636553D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- magnetic carrier
- binding magnetic
- isolation
- acid isolation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Revoked
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/01—Pretreatment specially adapted for magnetic separation by addition of magnetic adjuvants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y25/00—Nanomagnetism, e.g. magnetoimpedance, anisotropic magnetoresistance, giant magnetoresistance or tunneling magnetoresistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/0036—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
- H01F1/0045—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
- H01F1/0063—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use in a non-magnetic matrix, e.g. granular solids
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/01—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials
- H01F1/03—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity
- H01F1/032—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials
- H01F1/10—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites, e.g. [(Ba,Sr)O(Fe2O3)6] ferrites with hexagonal structure
- H01F1/11—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites, e.g. [(Ba,Sr)O(Fe2O3)6] ferrites with hexagonal structure in the form of particles
- H01F1/111—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties of inorganic materials characterised by their coercivity of hard-magnetic materials non-metallic substances, e.g. ferrites, e.g. [(Ba,Sr)O(Fe2O3)6] ferrites with hexagonal structure in the form of particles with a non-magnetic core
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01F—MAGNETS; INDUCTANCES; TRANSFORMERS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES
- H01F1/00—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties
- H01F1/0036—Magnets or magnetic bodies characterised by the magnetic materials therefor; Selection of materials for their magnetic properties showing low dimensional magnetism, i.e. spin rearrangements due to a restriction of dimensions, e.g. showing giant magnetoresistivity
- H01F1/0045—Zero dimensional, e.g. nanoparticles, soft nanoparticles for medical/biological use
- H01F1/0054—Coated nanoparticles, e.g. nanoparticles coated with organic surfactant
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Nanotechnology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Power Engineering (AREA)
- Urology & Nephrology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP17248195 | 1995-07-07 | ||
JP7172481A JP2965131B2 (ja) | 1995-07-07 | 1995-07-07 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69636553D1 true DE69636553D1 (de) | 2006-10-26 |
DE69636553T2 DE69636553T2 (de) | 2007-05-31 |
Family
ID=37068234
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69636553T Revoked DE69636553T2 (de) | 1995-07-07 | 1996-07-05 | Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP1260595B1 (de) |
DE (1) | DE69636553T2 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112007605A (zh) * | 2019-05-30 | 2020-12-01 | 苏州海狸生物医学工程有限公司 | 一种用于核酸提取的羟基纳米磁珠及其制备方法 |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7524630B2 (en) | 2002-04-22 | 2009-04-28 | University Of Florida Research Foundation, Inc. | Functionalized nanoparticles and methods of use |
US7601491B2 (en) | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
US20040157219A1 (en) * | 2003-02-06 | 2004-08-12 | Jianrong Lou | Chemical treatment of biological samples for nucleic acid extraction and kits therefor |
US8409463B1 (en) | 2007-07-16 | 2013-04-02 | University Of Central Florida Research Foundation, Inc. | Aqueous method for making magnetic iron oxide nanoparticles |
US9057094B1 (en) | 2007-10-25 | 2015-06-16 | University Of Central Florida Research Foundation, Inc. | Nanoparticle-mediated methods for antimicrobial susceptibility testing of bacteria |
EP2345719A1 (de) | 2010-01-18 | 2011-07-20 | Qiagen GmbH | Verfahren zur Isolierung kleiner RNA |
EP2407540A1 (de) | 2010-07-15 | 2012-01-18 | Qiagen GmbH | Verfahren zur Reinigung von Nukleinsäure |
US9109249B2 (en) | 2011-05-19 | 2015-08-18 | University Of Central Florida Research Foundation, Inc. | Microbe detection via hybridizing magnetic relaxation nanosensors |
WO2013024072A1 (en) | 2011-08-12 | 2013-02-21 | Qiagen Gmbh | Method for isolating nucleic acids |
EP2634254A1 (de) | 2012-02-29 | 2013-09-04 | QIAGEN GmbH | Verfahren zur Isolierung von Nukleinsäuren aus Nahrungsmittelproben |
DE102012012523B4 (de) * | 2012-06-26 | 2015-02-12 | Magnamedics Gmbh | Reinigung von Nukleinsäuren |
US20150225712A1 (en) | 2012-08-21 | 2015-08-13 | Qiagen Gmbh | Method for isolating nucleic acids from a formaldehyde releaser stabilized sample |
EP2888354B1 (de) | 2012-08-21 | 2020-04-01 | Qiagen GmbH | Stabilisierung von viruspartikeln und viralen nukleinsäuren in einer biologischen probe |
AU2013310861B2 (en) | 2012-09-03 | 2019-02-14 | Qiagen Gmbh | Method for isolating RNA including small RNA with high yield |
US10745686B2 (en) | 2013-02-08 | 2020-08-18 | Qiagen Gmbh | Method for separating DNA by size |
JP2015073485A (ja) * | 2013-10-09 | 2015-04-20 | セイコーエプソン株式会社 | 核酸増幅方法、核酸抽出用デバイス、核酸増幅反応用カートリッジ、及び核酸増幅反応用キット |
EP3059312A1 (de) | 2015-02-20 | 2016-08-24 | QIAGEN GmbH | Nukleinsäureextraktionsverfahren |
US10794859B2 (en) | 2015-06-01 | 2020-10-06 | Qiagen Gmbh | Electrophoresis assisted method and device for purifying a charged target molecule from a sample |
EP3303580B1 (de) | 2015-06-01 | 2020-11-18 | Qiagen GmbH | Elektrophoreseunterstütztes verfahren zur reinigung von ziel-nukleinsäure mittels ansatz der verzögerten elution |
WO2016193490A1 (en) | 2015-06-05 | 2016-12-08 | Qiagen Gmbh | Method for separating dna by size |
CN105214596B (zh) * | 2015-09-23 | 2017-10-31 | 郑州大学 | 一种用于动物组织核酸提取的磁性微球 |
CN105112404B (zh) * | 2015-09-23 | 2018-02-27 | 河南中标检测服务有限公司 | 一种动物组织核酸提取液的制备方法 |
US20210087606A1 (en) * | 2017-07-14 | 2021-03-25 | Capitalbio Corporation | Solid phase extraction material and its use for nucleic acid enrichment and detection |
CN111148834B (zh) | 2017-09-27 | 2024-06-11 | 凯杰有限公司 | 以高收率分离rna的方法 |
AU2019306640B2 (en) * | 2018-07-19 | 2022-09-22 | Beckman Coulter, Inc. | Magnetic particles |
CN112604645A (zh) * | 2020-12-10 | 2021-04-06 | 广东省测试分析研究所(中国广州分析测试中心) | 一种纳米磁粒及其制备方法和应用 |
CN117126842A (zh) * | 2022-05-27 | 2023-11-28 | 杭州诺辉健康科技有限公司 | 利用多孔纳米材料进行核酸提取和纯化的方法和试剂 |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4554088A (en) * | 1983-05-12 | 1985-11-19 | Advanced Magnetics Inc. | Magnetic particles for use in separations |
WO1989004373A1 (en) * | 1987-10-26 | 1989-05-18 | Baxter International Inc. | Process for producing magnetically responsive polymer particles and application thereof |
EP0343934B1 (de) * | 1988-05-24 | 1995-01-25 | Anagen (U.K.) Limited | Magnetisch anziehbare Teilchen und Herstellungsverfahren |
WO1990006045A2 (en) * | 1988-11-21 | 1990-06-14 | Dynal As | Nucleic acid probes |
NL8900725A (nl) * | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
AU8951191A (en) * | 1990-10-29 | 1992-05-26 | Dekalb Plant Genetics | Isolation of biological materials using magnetic particles |
US5610274A (en) * | 1991-11-20 | 1997-03-11 | Cpg, Inc. | Production and use of magnetic porous inorganic materials |
GB9223334D0 (en) * | 1992-11-06 | 1992-12-23 | Hybaid Ltd | Magnetic solid phase supports |
AU7375794A (en) * | 1993-07-28 | 1995-02-28 | Akzo Nobel N.V. | Process for isolating nucleic acid from gram positive microorganisms |
EP0723549B1 (de) * | 1993-08-30 | 2003-12-17 | Promega Corporation | Zusammensetzungen und verfahren zur reinigung von nukleinsäuren |
-
1996
- 1996-07-05 DE DE69636553T patent/DE69636553T2/de not_active Revoked
- 1996-07-05 EP EP02012236A patent/EP1260595B1/de not_active Revoked
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112007605A (zh) * | 2019-05-30 | 2020-12-01 | 苏州海狸生物医学工程有限公司 | 一种用于核酸提取的羟基纳米磁珠及其制备方法 |
Also Published As
Publication number | Publication date |
---|---|
EP1260595B1 (de) | 2006-09-13 |
EP1260595A3 (de) | 2002-12-18 |
EP1260595A2 (de) | 2002-11-27 |
DE69636553T2 (de) | 2007-05-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE69636553D1 (de) | Nukleinsäuren bindender magnetischer Träger und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung | |
EP0757106A3 (de) | Nukleinsäuren magnetischer Binder und Verfahren für die Nukleinsäureisolierung unter dessen Verwendung | |
DE69619864T2 (de) | Verfahren zur amplifizierung spezifischer nukleinsäuresequenzen | |
DE69519809D1 (de) | Bandulierungssystem und verfahren zu dessen verwendung | |
HUP9602289A2 (en) | Method for amplifying nucleic acid | |
DE59609524D1 (de) | Verfahren zur Genexpressionsanalyse | |
DE69630732D1 (de) | Mehrzelliges Dämpfungselement und Verfahren zur dessen Herstellung | |
DE59307620D1 (de) | Testträger zur Analytbestimmung sowie Verfahren zu dessen Herstellung | |
DE59511093D1 (de) | Verfahren zum direkten Nachweisen weniger Nukleinsäurestränge | |
DE69534886D1 (de) | Verfahren zur Isolierung von Zellkomponenten | |
DE59900006D1 (de) | Verfahren zur Isolierung von gekrümmten Flächen | |
DE69531765D1 (de) | Verfahren zur heimatkanaltrennnung | |
ATE212380T1 (de) | Verfahren zur herstellung von nikotinsäureamid | |
DE59610517D1 (de) | Befestigungselement und Verfahren zu dessen Herstellung | |
DE69920967D1 (de) | Verfahren zur cvd unter verwendung von bi-alkoxiden | |
DE69501893D1 (de) | Verfahren zur genetischen prüfung | |
DE69601540D1 (de) | Verfahren zur oxychlorierung | |
DE69823174D1 (de) | Verfahren zur cvd bei niedrigen temperaturen unter verwendung von bi-carboxylaten | |
DE59601365D1 (de) | Verfahren zum Isolieren von Formspulen | |
DE69605561D1 (de) | Verfahren zur beschichtung | |
DE59301973D1 (de) | Pipettierhilfe und verfahren zu ihrer herstellung | |
DE69728226D1 (de) | Träger für Bioreaktor sowie Verfahren zu dessen Herstellung | |
DE69637516D1 (de) | Verfahren für die Amplifizierung von Nukleinsäure-Sequenzen | |
DE69609963T2 (de) | Verfahren zur iodierung | |
DE69840011D1 (de) | e und Verfahren zu dessen Herstellung |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8363 | Opposition against the patent | ||
8331 | Complete revocation |