DE69334157D1 - Expressionsvektor pUC NT und ihre Verwendung - Google Patents
Expressionsvektor pUC NT und ihre VerwendungInfo
- Publication number
- DE69334157D1 DE69334157D1 DE69334157T DE69334157T DE69334157D1 DE 69334157 D1 DE69334157 D1 DE 69334157D1 DE 69334157 T DE69334157 T DE 69334157T DE 69334157 T DE69334157 T DE 69334157T DE 69334157 D1 DE69334157 D1 DE 69334157D1
- Authority
- DE
- Germany
- Prior art keywords
- expression vector
- vector puc
- puc
- expression
- vector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/22—Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP21269292 | 1992-08-10 | ||
JP21269292 | 1992-08-10 | ||
JP34007892 | 1992-12-21 | ||
JP34007892 | 1992-12-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
DE69334157D1 true DE69334157D1 (de) | 2007-09-06 |
DE69334157T2 DE69334157T2 (de) | 2008-04-17 |
Family
ID=26519366
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69333668T Expired - Fee Related DE69333668T2 (de) | 1992-08-10 | 1993-08-05 | Für eine decarbamylase mit verbesserter thermostabilität kodierende dna und ihre verwendung |
DE69334157T Expired - Lifetime DE69334157T2 (de) | 1992-08-10 | 1993-08-05 | Expressionsvektor pUC NT und ihre Verwendung |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE69333668T Expired - Fee Related DE69333668T2 (de) | 1992-08-10 | 1993-08-05 | Für eine decarbamylase mit verbesserter thermostabilität kodierende dna und ihre verwendung |
Country Status (9)
Country | Link |
---|---|
US (1) | US6083752A (de) |
EP (2) | EP0610517B1 (de) |
JP (1) | JP3438890B2 (de) |
KR (1) | KR100266920B1 (de) |
CN (2) | CN1040025C (de) |
DE (2) | DE69333668T2 (de) |
ES (1) | ES2231772T3 (de) |
SG (1) | SG54275A1 (de) |
WO (1) | WO1994003613A1 (de) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0628637B1 (de) * | 1992-10-05 | 2001-06-13 | Kanegafuchi Chemical Industry Co., Ltd. | Verfahren zur herstellung von d-alpha-aminosäuren |
JPH07114789B2 (ja) * | 1993-08-26 | 1995-12-13 | 工業技術院長 | 上肢動作補助機構 |
IT1269321B (it) * | 1994-04-15 | 1997-03-26 | Eniricerche Spa | Mutanti stabili della d-n- -carbamilasi |
US5962279A (en) * | 1994-06-24 | 1999-10-05 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Process for producing D-amino acids with composite immobilized enzyme preparation |
IT1277125B1 (it) * | 1995-12-21 | 1997-11-04 | Eniricerche Spa | Mutanti termostabili della d-n-alfa-carbamilasi |
JP2001069981A (ja) * | 1999-08-31 | 2001-03-21 | Kanegafuchi Chem Ind Co Ltd | デカルバミラーゼの立体構造およびその利用法 |
JP4561009B2 (ja) | 2001-07-10 | 2010-10-13 | 味の素株式会社 | D−ヒダントインハイドロラーゼをコードするdna、n−カルバミル−d−アミノ酸ハイドロラーゼをコードするdna、該遺伝子を含む組み換えdna、該組み換えdnaにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、d−アミノ酸の製造方法 |
ATE482274T1 (de) * | 2004-02-04 | 2010-10-15 | Kaneka Corp | Polypeptid mit amidaseaktivität und dessen gen |
WO2006013802A1 (ja) | 2004-08-06 | 2006-02-09 | Kaneka Corporation | エナンチオマー豊富化化合物の製造方法 |
WO2006046455A1 (ja) | 2004-10-27 | 2006-05-04 | Kaneka Corporation | 新規カルボニル還元酵素、その遺伝子、およびその利用法 |
JPWO2006129628A1 (ja) | 2005-05-31 | 2009-01-08 | 株式会社カネカ | 光学活性2−置換プロパナール誘導体の製造法 |
EP1918375B1 (de) | 2005-08-02 | 2015-03-11 | Kaneka Corporation | D-aminosäure-oxidase und verfahren zur herstellung von l-aminosäure, 2-oxosäure oder cyclischem imin |
WO2007040272A1 (ja) * | 2005-10-06 | 2007-04-12 | Kaneka Corporation | D-(4-アミノメチル)フェニルアラニン誘導体の製造法 |
US20110143406A1 (en) | 2006-02-14 | 2011-06-16 | Kaneka Corporation | Novel (S,S) -Butanediol Dehydrogenase, Gene for the Same, and Use of the Same |
WO2007099764A1 (ja) * | 2006-02-28 | 2007-09-07 | Kaneka Corporation | 新規カルボニル還元酵素、その遺伝子、およびそれらを利用した光学活性アルコールの製造方法 |
JP5410089B2 (ja) | 2006-05-29 | 2014-02-05 | 株式会社カネカ | 光学活性アミン化合物の製造方法、組換えベクター、及び該ベクターを含む形質転換体。 |
US9273332B2 (en) | 2006-10-12 | 2016-03-01 | Kaneka Corporation | Method for production of L-amino acid |
KR100858913B1 (ko) * | 2007-03-09 | 2008-09-17 | 한국과학기술원 | 부위특이적 변이에 의해 제조된 고수율의 l-쓰레오닌생산균주 및 이를 이용한 l-쓰레오닌의 제조방법 |
DK2202303T3 (en) | 2007-09-26 | 2017-02-06 | Kaneka Corp | NEW GLUCOSEDE HYDROGENASE |
WO2010067578A1 (ja) | 2008-12-09 | 2010-06-17 | 株式会社カネカ | 新規なアミノ酸脱水素酵素、およびl-アミノ酸、2-オキソ酸、又はd-アミノ酸の製造方法 |
WO2010123062A1 (ja) | 2009-04-23 | 2010-10-28 | 株式会社カネカ | (r)-3-キヌクリジノールの製造方法 |
JP5889785B2 (ja) | 2010-04-22 | 2016-03-22 | 株式会社カネカ | 改変型カルボニル還元酵素、その遺伝子、およびそれらを利用した光学活性アルコールの製造方法 |
JP5936545B2 (ja) | 2010-09-28 | 2016-06-22 | 株式会社カネカ | グルタミン酸に高活性を示す新規アミノ基転移酵素、およびこれをコードする遺伝子、ならびにこれらの利用法 |
DK2639216T3 (en) | 2010-11-09 | 2018-09-24 | Kaneka Corp | Halogenated Indones and Methods for Preparation of Optically Active Indanones or Optically Active Indanols Using These |
EP2684953B1 (de) | 2011-03-11 | 2017-06-28 | Kaneka Corporation | Modifizierte aminotransferase, gen davon und verfahren zur herstellung einer optisch aktiven aminoverbindung damit |
SG11201508053WA (en) | 2013-03-28 | 2015-10-29 | Kaneka Corp | Modified carbonyl reducing enzyme and gene |
EP3536782A4 (de) | 2016-11-01 | 2019-10-02 | Kaneka Corporation | Modifiziertes enzym und verwendung davon |
CN110603331A (zh) | 2017-05-01 | 2019-12-20 | 株式会社钟化 | 利用了atp的物质的制造方法 |
CN114807103B (zh) * | 2022-06-10 | 2023-07-04 | 铜陵利夫生物科技有限公司 | 一种氨基甲酰基水解酶突变体及基因和应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3819463A1 (de) * | 1988-06-08 | 1989-12-14 | Behringwerke Ag | Expressionsvektoren zur herstellung unfusionierter proteine in mikroorganismen |
JPH01320991A (ja) * | 1988-06-20 | 1989-12-27 | Kanegafuchi Chem Ind Co Ltd | D−ホモフエニルアラニン類の製造方法 |
DE3841454A1 (de) * | 1988-12-09 | 1990-06-13 | Degussa | Verfahren zur ortsspezifischen mutagenese von dna und entwicklung von plasmidvektoren |
US5824522A (en) * | 1990-12-07 | 1998-10-20 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Recombinant decarbamylases for producing D-α-amino acids |
US5565344A (en) * | 1990-12-07 | 1996-10-15 | Kanegafuchi Kagaku Kogyo Kabushiki Kaisha | Process for production of D-α-amino acids |
JPH09210579A (ja) * | 1996-01-31 | 1997-08-12 | Calsonic Corp | オイルクーラのスタッドボルト取付部構造 |
-
1993
- 1993-08-05 DE DE69333668T patent/DE69333668T2/de not_active Expired - Fee Related
- 1993-08-05 EP EP94906767A patent/EP0610517B1/de not_active Expired - Lifetime
- 1993-08-05 JP JP50518694A patent/JP3438890B2/ja not_active Expired - Fee Related
- 1993-08-05 SG SG1996007004A patent/SG54275A1/en unknown
- 1993-08-05 ES ES94906767T patent/ES2231772T3/es not_active Expired - Lifetime
- 1993-08-05 EP EP00113030A patent/EP1043398B1/de not_active Expired - Lifetime
- 1993-08-05 KR KR1019940701155A patent/KR100266920B1/ko not_active IP Right Cessation
- 1993-08-05 DE DE69334157T patent/DE69334157T2/de not_active Expired - Lifetime
- 1993-08-05 WO PCT/JP1993/001101 patent/WO1994003613A1/ja active IP Right Grant
- 1993-08-10 CN CN93109292A patent/CN1040025C/zh not_active Expired - Fee Related
-
1997
- 1997-03-05 CN CN97102935A patent/CN1108383C/zh not_active Expired - Fee Related
- 1997-06-16 US US08/876,398 patent/US6083752A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
DE69333668D1 (de) | 2004-11-25 |
EP0610517A1 (de) | 1994-08-17 |
WO1994003613A1 (en) | 1994-02-17 |
SG54275A1 (en) | 1998-11-16 |
CN1083528A (zh) | 1994-03-09 |
DE69334157T2 (de) | 2008-04-17 |
CN1108383C (zh) | 2003-05-14 |
EP0610517A4 (en) | 1998-06-10 |
CN1168415A (zh) | 1997-12-24 |
ES2231772T3 (es) | 2005-05-16 |
US6083752A (en) | 2000-07-04 |
EP1043398A1 (de) | 2000-10-11 |
EP0610517B1 (de) | 2004-10-20 |
EP1043398B1 (de) | 2007-07-25 |
DE69333668T2 (de) | 2006-02-23 |
JP3438890B2 (ja) | 2003-08-18 |
KR100266920B1 (ko) | 2003-12-31 |
CN1040025C (zh) | 1998-09-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition |