DE69331908D1 - Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen - Google Patents

Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen

Info

Publication number
DE69331908D1
DE69331908D1 DE69331908T DE69331908T DE69331908D1 DE 69331908 D1 DE69331908 D1 DE 69331908D1 DE 69331908 T DE69331908 T DE 69331908T DE 69331908 T DE69331908 T DE 69331908T DE 69331908 D1 DE69331908 D1 DE 69331908D1
Authority
DE
Germany
Prior art keywords
expression
plant cells
foreign genes
transcription initiation
initiation region
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69331908T
Other languages
English (en)
Other versions
DE69331908T2 (de
Inventor
F Thomson
L Spiker
C Allen
E Hall
C Childs
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
North Carolina State University
University of California
Original Assignee
North Carolina State University
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by North Carolina State University, University of California filed Critical North Carolina State University
Publication of DE69331908D1 publication Critical patent/DE69331908D1/de
Application granted granted Critical
Publication of DE69331908T2 publication Critical patent/DE69331908T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
DE69331908T 1992-10-05 1993-09-27 Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen Expired - Lifetime DE69331908T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95642092A 1992-10-05 1992-10-05
PCT/US1993/009179 WO1994007902A1 (en) 1992-10-05 1993-09-27 Method for increasing expression and reducing expression variability of foreign genes in plant cells

Publications (2)

Publication Number Publication Date
DE69331908D1 true DE69331908D1 (de) 2002-06-13
DE69331908T2 DE69331908T2 (de) 2002-12-12

Family

ID=25498228

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69331908T Expired - Lifetime DE69331908T2 (de) 1992-10-05 1993-09-27 Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen

Country Status (10)

Country Link
US (1) US5773689A (de)
EP (1) EP0663921B1 (de)
AT (1) ATE217319T1 (de)
AU (1) AU673859B2 (de)
CA (1) CA2147006C (de)
DE (1) DE69331908T2 (de)
DK (1) DK0663921T3 (de)
ES (1) ES2176208T3 (de)
PT (1) PT663921E (de)
WO (1) WO1994007902A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6239328B1 (en) 1992-10-05 2001-05-29 North Carolina State University Method for reducing expression variability of transgenes in plant cells
US6040185A (en) * 1993-04-19 2000-03-21 Novartis Ag Genetic stabilizing elements
IL109333A0 (en) * 1993-04-19 1994-07-31 Sandoz Ag Genetic stabilizing elements
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US6037525A (en) * 1996-08-01 2000-03-14 North Carolina State University Method for reducing expression variability of transgenes in plant cells
CA2263891A1 (en) * 1996-10-17 1998-04-23 E.I. Du Pont De Nemours And Company Enhanced transgene expression in a population of monocot cells employing scaffold attachment regions
EP0970230A1 (de) * 1997-03-28 2000-01-12 Mycogen Plant Sciences, Inc. Verbessertes sar pflanzentransformations-verfahren
BR9810075A (pt) * 1997-06-03 2000-09-19 Univ North Carolina State Processo para redução da variabilidade de expressão de transgênicos em células vegetais
US6245974B1 (en) * 1997-08-06 2001-06-12 North Carolina State University Matrix attachment regions
US6518485B1 (en) * 1998-06-04 2003-02-11 Westvaco Corporation Particle-mediated conifer transformation
WO2000006757A1 (en) * 1998-07-31 2000-02-10 Mycogen Plant Science, Inc. Improved plant transformation process by scaffold attachment regions (sar)
WO2000018938A1 (en) * 1998-09-29 2000-04-06 Pioneer Hi-Bred International, Inc. Mar/sar elements flanking rsyn7-driven construct
US6482646B1 (en) 1998-11-06 2002-11-19 E. I. Du Pont De Nemours And Company Plant proteins that interact with nuclear matrix proteins and function as transcriptional activators
JP4544748B2 (ja) * 1998-12-01 2010-09-15 ダウ・アグロサイエンス・エル・エル・シー 植物細胞内に導入された遺伝子の発現を増加するための人工マトリックス付着領域
US6395962B1 (en) 1999-06-22 2002-05-28 University Of South Carolina Enhancing expression of a silenced target sequence in plants using plant viral enhancers and amplicons
US7126041B1 (en) 1999-12-10 2006-10-24 North Carolina State Unversity High efficiency gene targeting in plants
KR100408844B1 (ko) 2000-07-29 2003-12-06 한국산업기술평가원 동물세포 발현벡터
WO2002061039A2 (en) * 2000-10-25 2002-08-08 Washington State University Research Foundation Thuja plicata dirigent protein promotors
DK1395669T3 (da) 2001-01-26 2009-11-16 Selexis Sa Matriks bindingsregioner og fremgangsmåder til anvendelse af disse
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
EP1279737A1 (de) 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformationsmethode zur Erzeugung von Pflanzen ohne Selektionsmarker
US20040016014A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
US20040016015A1 (en) * 2002-07-17 2004-01-22 Nguyen Thanh-Tuyen T. High efficiency germline transformation system
OA13241A (en) * 2003-08-19 2007-01-31 22Nd Century Ltd Llc Reduced-exposure tobacco products.
AU2004284220B2 (en) 2003-10-24 2010-10-14 Selexis S.A. High efficiency gene transfer and expression in mammalian cells by a multiple transfection procedure of matrix attachment region sequences
CN101355961A (zh) 2005-11-04 2009-01-28 美国陶氏益农公司 使用重组植物悬浮培养物制备疫苗主细胞系
EP2099916A2 (de) 2006-12-22 2009-09-16 Dow AgroSciences LLC Aus pflanzen hergestellte west-nil-virus-impfstoffe, vektoren und pflanzencodonoptimierte sequenzen
CA2822720C (en) * 2010-12-24 2020-05-12 Bayer Cropscience Nv Brassica plant comprising a mutant alcatraz allele
CN104024412A (zh) * 2011-09-15 2014-09-03 巴斯夫植物科学有限公司 用于植物中可靠基因表达的调节核酸分子
EP3782465B1 (de) 2019-08-23 2023-08-30 Universität Potsdam Transgenes zebrafischmodell für schlaganfall

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5122466A (en) * 1989-06-13 1992-06-16 North Carolina State University Ballistic transformation of conifers
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
JPH05505525A (ja) * 1990-03-05 1993-08-19 ジ・アップジョン・カンパニー 種子特異的調節配列類を介する蛋白発現
US5187267A (en) * 1990-06-19 1993-02-16 Calgene, Inc. Plant proteins, promoters, coding sequences and use
MX9200621A (es) * 1991-02-14 1993-02-01 Du Pont Gen de una proteina con alto contenido de azufre de una semilla y metodo para aumentar el contenido de azufre en aminoacidos de las plantas.
DE69332974T2 (de) * 1992-03-19 2004-05-19 E.I. Du Pont De Nemours And Co., Wilmington Nukleinsäurefragmente und verfahren zur steigerung des lysin- und threoningehaltes der pflanzensamen
IL109333A0 (en) * 1993-04-19 1994-07-31 Sandoz Ag Genetic stabilizing elements

Also Published As

Publication number Publication date
PT663921E (pt) 2002-10-31
EP0663921A1 (de) 1995-07-26
WO1994007902A1 (en) 1994-04-14
US5773689A (en) 1998-06-30
EP0663921B1 (de) 2002-05-08
AU5165593A (en) 1994-04-26
DE69331908T2 (de) 2002-12-12
ATE217319T1 (de) 2002-05-15
CA2147006C (en) 2004-11-30
CA2147006A1 (en) 1994-04-14
EP0663921A4 (de) 1996-07-17
AU673859B2 (en) 1996-11-28
DK0663921T3 (da) 2002-09-02
ES2176208T3 (es) 2002-12-01

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