DE69221423T2 - Verbesserte Fluoreszenz-Erfassung von Proben in einem Kapillarrohr - Google Patents

Verbesserte Fluoreszenz-Erfassung von Proben in einem Kapillarrohr

Info

Publication number
DE69221423T2
DE69221423T2 DE1992621423 DE69221423T DE69221423T2 DE 69221423 T2 DE69221423 T2 DE 69221423T2 DE 1992621423 DE1992621423 DE 1992621423 DE 69221423 T DE69221423 T DE 69221423T DE 69221423 T2 DE69221423 T2 DE 69221423T2
Authority
DE
Germany
Prior art keywords
samples
capillary tube
fluorescence detection
improved fluorescence
improved
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE1992621423
Other languages
English (en)
Other versions
DE69221423D1 (de
Inventor
Stephen L Pentoney
Wilbur Kaye
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beckman Coulter Inc
Original Assignee
Beckman Instruments Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/769,422 external-priority patent/US5273638A/en
Application filed by Beckman Instruments Inc filed Critical Beckman Instruments Inc
Application granted granted Critical
Publication of DE69221423D1 publication Critical patent/DE69221423D1/de
Publication of DE69221423T2 publication Critical patent/DE69221423T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00—Photometry, e.g. photographic exposure meter
    • G01J1/02—Details
    • G01J1/04—Optical or mechanical part supplementary adjustable parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00—Photometry, e.g. photographic exposure meter
    • G01J1/02—Details
    • G01J1/04—Optical or mechanical part supplementary adjustable parts
    • G01J1/0407—Optical elements not provided otherwise, e.g. manifolds, windows, holograms, gratings
    • G01J1/0414—Optical elements not provided otherwise, e.g. manifolds, windows, holograms, gratings using plane or convex mirrors, parallel phase plates, or plane beam-splitters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J1/00—Photometry, e.g. photographic exposure meter
    • G01J1/02—Details
    • G01J1/04—Optical or mechanical part supplementary adjustable parts
    • G01J1/0407—Optical elements not provided otherwise, e.g. manifolds, windows, holograms, gratings
    • G01J1/0422—Optical elements not provided otherwise, e.g. manifolds, windows, holograms, gratings using light concentrators, collectors or condensers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N2021/1738—Optionally different kinds of measurements; Method being valid for different kinds of measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N2021/4702—Global scatter; Total scatter, excluding reflections
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/84—Systems specially adapted for particular applications
    • G01N21/85—Investigating moving fluids or granular solids

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE1992621423 1991-09-30 1992-09-29 Verbesserte Fluoreszenz-Erfassung von Proben in einem Kapillarrohr Expired - Fee Related DE69221423T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/769,422 US5273638A (en) 1991-09-30 1991-09-30 Nucleotide sequence determination employing matched dideoxynucleotide terminator concentrations
US77282391A 1991-10-08 1991-10-08

Publications (2)

Publication Number Publication Date
DE69221423D1 DE69221423D1 (de) 1997-09-11
DE69221423T2 true DE69221423T2 (de) 1998-03-19

Family

ID=27118169

Family Applications (1)

Application Number Title Priority Date Filing Date
DE1992621423 Expired - Fee Related DE69221423T2 (de) 1991-09-30 1992-09-29 Verbesserte Fluoreszenz-Erfassung von Proben in einem Kapillarrohr

Country Status (3)

Country Link
EP (1) EP0539743B1 (de)
JP (1) JPH05209832A (de)
DE (1) DE69221423T2 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012108158A1 (de) * 2012-09-03 2014-03-06 Johann Wolfgang Goethe-Universität Kapillarzelle, Anordnung und Verfahren zur Aufnahme, zur Positionierung und zur Untersuchung einer mikroskopischen Probe

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO932088L (no) * 1993-06-08 1995-01-05 Oddbjoern Gjelsnes Anordning for anvendelse ved væskeströmscytometri
SE9404274D0 (sv) * 1994-12-08 1994-12-08 Pharmacia Biotech Ab Anordning vid en gelelektroforesapparat
US5614726A (en) * 1995-03-23 1997-03-25 Beckman Instruments, Inc. Automated optical alignment system and method using Raman scattering of capillary tube contents
US5675155A (en) * 1995-04-26 1997-10-07 Beckman Instruments, Inc. Multicapillary fluorescent detection system
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
US5545901A (en) * 1995-08-23 1996-08-13 Beckman Instruments, Inc. Automated optical alignment using a galvometric scanner
US6486947B2 (en) * 1998-07-22 2002-11-26 Ljl Biosystems, Inc. Devices and methods for sample analysis
JP4392990B2 (ja) * 1998-05-09 2010-01-06 レニショウ パブリック リミテッド カンパニー 電子顕微鏡および分光システム
DE10114200A1 (de) 2001-03-23 2002-09-26 Profil Verbindungstechnik Gmbh Funktionselement, Zusammenbauteil bestehend aus einem Blechteil und einem Funktionselement sowie Verfahren zur Anbringung eines Funktionselementes an ein Blechteil
DE10243759B4 (de) 2002-09-20 2011-08-11 PROFIL Verbindungstechnik GmbH & Co. KG, 61381 Verfahren zur Erzeugung einer elektrisch leitenden Verbindung zwischen einer elektrischen Anschlusseinrichtung wie ein Kabelschuh und einem Blechteil, Befestigungselement und Zusammenbauteil
US6995841B2 (en) * 2001-08-28 2006-02-07 Rice University Pulsed-multiline excitation for color-blind fluorescence detection
US7518727B2 (en) 2007-02-28 2009-04-14 Beckman Coulter, Inc. Multicapillary multilaser detection system
DE202008009859U1 (de) * 2007-12-21 2009-03-05 Berthold Technologies Gmbh & Co. Kg Vorrichtung zur wahlweisen Messung von insbesondere Lumineszenz- und/oder Fluoreszenzstrahlung
WO2010080642A1 (en) * 2008-12-18 2010-07-15 Biovigilant Systems, Inc. Compact detector for simultaneous particle size and fluorescence detection
JP2012085605A (ja) * 2010-10-22 2012-05-10 Sony Corp 核酸増幅反応装置、核酸増幅反応装置に用いる基板、及び核酸増幅反応方法
JP5787390B2 (ja) * 2011-04-06 2015-09-30 インスタント バイオスキャン， エルエルシー 微生物検出装置及び方法
JP2013160672A (ja) * 2012-02-07 2013-08-19 Sony Corp 蛍光検出用光学系及び微小粒子分析装置
JP6113549B2 (ja) * 2013-03-28 2017-04-12 株式会社日立ハイテクノロジーズ 電気泳動装置
US20220412917A1 (en) * 2019-11-14 2022-12-29 Dh Technologies Development Pte. Ltd. Dual Mode Scanning Optical System for Capillary Electrophoresis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3985441A (en) * 1974-03-13 1976-10-12 Schoeffel Instrument Corporation Multi-channel spectral analyzer for liquid chromatographic separations
GB2155176B (en) * 1984-01-16 1988-05-11 California Inst Of Techn Sequencing dna
US4838688A (en) * 1987-10-16 1989-06-13 Robert Rhoads High sensitivity chromatography detector
US4854700A (en) * 1988-06-15 1989-08-08 The Dow Chemical Company Column holder for on-column photometric detection
FR2649487B1 (fr) * 1989-06-21 1991-09-20 Europhor Sa Capillaire d'electrophorese a dispositif optique incorpore

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102012108158A1 (de) * 2012-09-03 2014-03-06 Johann Wolfgang Goethe-Universität Kapillarzelle, Anordnung und Verfahren zur Aufnahme, zur Positionierung und zur Untersuchung einer mikroskopischen Probe
DE102012108158B4 (de) * 2012-09-03 2016-03-17 Johann Wolfgang Goethe-Universität Kapillarzelle, Anordnung und Verfahren zur Aufnahme, zur Positionierung und zur Untersuchung einer mikroskopischen Probe
US9816916B2 (en) 2012-09-03 2017-11-14 Johann Wolfgang Goethe-Universitaet Capillary cell, arrangement and method for accommodating, positioning and examining a microscopic specimen

Also Published As

Publication number Publication date
EP0539743B1 (de) 1997-08-06
EP0539743A1 (de) 1993-05-05
JPH05209832A (ja) 1993-08-20
DE69221423D1 (de) 1997-09-11

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee