DE69132273D1 - Verfahren und vorrichtung zur bestimmung von lebensdauer von fluoreszenz in der durchflusscytometrie - Google Patents

Verfahren und vorrichtung zur bestimmung von lebensdauer von fluoreszenz in der durchflusscytometrie

Info

Publication number
DE69132273D1
DE69132273D1 DE69132273T DE69132273T DE69132273D1 DE 69132273 D1 DE69132273 D1 DE 69132273D1 DE 69132273 T DE69132273 T DE 69132273T DE 69132273 T DE69132273 T DE 69132273T DE 69132273 D1 DE69132273 D1 DE 69132273D1
Authority
DE
Germany
Prior art keywords
lifetime
fluorescence
determining
flow cytometry
cytometry
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69132273T
Other languages
English (en)
Inventor
R Lakowicz
W Berndt
A Hoffman
G Pinsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
University of Maryland at Baltimore
Original Assignee
Becton Dickinson and Co
University of Maryland at Baltimore
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co, University of Maryland at Baltimore filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of DE69132273D1 publication Critical patent/DE69132273D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1454—Optical arrangements using phase shift or interference, e.g. for improving contrast

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
DE69132273T 1990-10-10 1991-10-10 Verfahren und vorrichtung zur bestimmung von lebensdauer von fluoreszenz in der durchflusscytometrie Expired - Lifetime DE69132273D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59534390A 1990-10-10 1990-10-10
PCT/US1991/007259 WO1992007245A1 (en) 1990-10-10 1991-10-10 Method and apparatus for performing phase fluorescence lifetime measurements in flow cytometry

Publications (1)

Publication Number Publication Date
DE69132273D1 true DE69132273D1 (de) 2000-08-03

Family

ID=24382865

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69132273T Expired - Lifetime DE69132273D1 (de) 1990-10-10 1991-10-10 Verfahren und vorrichtung zur bestimmung von lebensdauer von fluoreszenz in der durchflusscytometrie

Country Status (4)

Country Link
US (1) US5504337A (de)
EP (1) EP0552241B1 (de)
DE (1) DE69132273D1 (de)
WO (1) WO1992007245A1 (de)

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Also Published As

Publication number Publication date
EP0552241A1 (de) 1993-07-28
EP0552241B1 (de) 2000-06-28
US5504337A (en) 1996-04-02
WO1992007245A1 (en) 1992-04-30

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