DE69131596T2 - Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen - Google Patents

Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen

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Publication number
DE69131596T2
DE69131596T2 DE69131596T DE69131596T DE69131596T2 DE 69131596 T2 DE69131596 T2 DE 69131596T2 DE 69131596 T DE69131596 T DE 69131596T DE 69131596 T DE69131596 T DE 69131596T DE 69131596 T2 DE69131596 T2 DE 69131596T2
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DE
Germany
Prior art keywords
dna
nucleic acid
construct
polymerase
dependent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69131596T
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English (en)
Other versions
DE69131596D1 (de
Inventor
Michael Urdea
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Bayer Corp
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Chiron Diagnostics Corp
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Publication of DE69131596D1 publication Critical patent/DE69131596D1/de
Publication of DE69131596T2 publication Critical patent/DE69131596T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/03—Herpetoviridae, e.g. pseudorabies virus
    • G01N2333/04—Varicella-zoster virus
    • G01N2333/045—Cytomegalovirus

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Communicable Diseases (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • AIDS & HIV (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69131596T 1990-01-10 1991-01-10 Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen Expired - Fee Related DE69131596T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46302290A 1990-01-10 1990-01-10
PCT/US1991/000213 WO1991010746A1 (en) 1990-01-10 1991-01-10 Dna-dependent rna polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays

Publications (2)

Publication Number Publication Date
DE69131596D1 DE69131596D1 (de) 1999-10-14
DE69131596T2 true DE69131596T2 (de) 1999-12-23

Family

ID=23838605

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69131596T Expired - Fee Related DE69131596T2 (de) 1990-01-10 1991-01-10 Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen

Country Status (8)

Country Link
EP (1) EP0510085B1 (de)
JP (2) JPH05503422A (de)
AT (1) ATE184321T1 (de)
CA (1) CA2070632A1 (de)
DE (1) DE69131596T2 (de)
IE (1) IE910081A1 (de)
PT (1) PT96458B (de)
WO (1) WO1991010746A1 (de)

Families Citing this family (32)

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US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US5856088A (en) * 1989-07-11 1999-01-05 Gen-Probe Incorporated Detection of human immunodeficiency virus type 1
US5780219A (en) * 1989-07-11 1998-07-14 Gen-Probe Incorporated Nucleic acid amplification oligonucleotides and probes to human hepatitis B virus
AU1741692A (en) * 1991-03-13 1992-10-21 Regents Of The University Of California, The Detection of 3'-azido-3'-deoxythymidine resistance
EP0648281A4 (de) * 1991-09-10 1997-06-04 Jack D Love Zielgerichte amplifikation von dna und rna und signalamplifikation.
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US6943034B1 (en) 1991-11-22 2005-09-13 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
FR2685346B1 (fr) * 1991-12-18 1994-02-11 Cis Bio International Procede de preparation d'arn double-brin, et ses applications.
DE69233630T2 (de) 1991-12-23 2007-05-31 Chiron Corp., Emeryville Sätze von HIV-Sonden zur Verwendung in Sandwich-Hydridisierungsassays in Lösungsphase
KR950701392A (ko) * 1992-05-06 1995-03-23 다니엘 엘. 카시안 인체 B형 간염 바이러스에 대한 핵산 증폭 올리고뉴클레오티드 및 프로브(Nucleic Acide Amplification Oligonucleotides and Probes to Human Hepatitis B Birus)
WO1993022461A1 (en) * 1992-05-06 1993-11-11 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
WO1993024658A1 (en) * 1992-05-29 1993-12-09 Gen Trak, Inc. Signal amplification probe and methods of use
AU4387193A (en) * 1992-05-29 1993-12-30 Abbott Laboratories Ligase chain reaction starting with rna sequences
JP4108118B2 (ja) * 1993-03-26 2008-06-25 ジェン−プローブ・インコーポレイテッド ヒト・免疫不全ウイルス１型の検出
US5736334A (en) * 1993-04-12 1998-04-07 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis B viral DNA
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US5733781A (en) * 1994-07-19 1998-03-31 Gen-Probe Incorporated Oligonucleotides and methods for inhibiting propagation of human immunodeficiency virus
US5753787A (en) * 1995-04-10 1998-05-19 Yale University Nucleic acids encoding ancylostoma secreted protein
ZA973367B (en) 1996-04-19 1997-11-18 Innogenetics Nv Method for typing and detecting HBV.
US6025133A (en) * 1996-12-30 2000-02-15 Gen-Probe Incorporated Promoter-sequestered oligonucleoside and method of use
US5962665A (en) * 1997-06-16 1999-10-05 Abbott Laboratories Nucleic acid primers and probes for detecting HIV-1 and HIV-2
US6001558A (en) * 1997-06-25 1999-12-14 Ortho Clinical Diagnostics, Inc. Amplification and detection of HIV-1 and/or HIV 2
ES2153807T3 (es) * 1997-08-08 2005-06-16 Biomerieux B.V. Secuencias de acido nucleico que se pueden utilizar como cebadores y sondas en la amplificacion y deteccion de todos los subtipos de vih-1.
DE19835856A1 (de) * 1998-08-07 2000-02-17 W Kurt Roth Verfahren zum Nachweis von HBV
JP2003517837A (ja) * 1999-12-21 2003-06-03 アールエヌエー−ライン オーワイ バクテリオファージファイ６〜ファイ１４由来のｒｎａポリメラーゼ類およびその使用
US6635428B2 (en) 2001-12-04 2003-10-21 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis B viral nucleic acids
EP1513958B1 (de) * 2002-06-14 2011-08-10 Gen-Probe Incorporated Zusammensetzungen zum nachweis von hepatitis-b-virus
US20110136678A1 (en) 2006-10-20 2011-06-09 Erwin Sablon Methodology for analysis of sequence variations within the hcv ns3/4a genomic region
EP2463389A1 (de) 2006-10-20 2012-06-13 Innogenetics N.V. Methodik zur Analyse von Sequenzvariationen innerhalb des HCV-NS5B-Genombereichs
GB0701253D0 (en) 2007-01-23 2007-02-28 Diagnostics For The Real World Nucleic acid amplification and testing
GB2455348B (en) * 2007-12-07 2012-09-12 Scorpion Patent Ltd A process for dry curing a joint of pork
CN116083392A (zh) * 2023-02-13 2023-05-09 深圳蓝晶生物科技有限公司 哺乳动物定量表达***

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GB8306426D0 (en) 1983-03-09 1983-04-13 Malcolm A D B Detecting polynucleotide sequence
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
US4868105A (en) 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
CA1339351C (en) 1987-10-15 1997-08-26 Michael S. Urdea Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
DE68908054T2 (de) 1988-01-21 1994-03-10 Genentech Inc Verstärkung und nachweis von nukleinsäuresequenzen.
JPH02131599A (ja) * 1988-07-15 1990-05-21 Toray Ind Inc 信号増幅ｄｎａ分子およびそれを用いる検出方法
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JP2939485B2 (ja) * 1988-09-08 1999-08-25 ザ・ソーク・インスチュート・フォーバイオロジカル・スタディーズ 複製ｒｎａに基づく増幅／検出系
CA2001110A1 (en) * 1988-11-18 1990-05-18 Zvi G. Loewy Compositions and process for amplifying and detecting nucleic acid sequences
IE911534A1 (en) * 1990-05-04 1991-11-06 Chiron Corp Protein-nucleic acid probes and immunoassays using same

Also Published As

Publication number Publication date
EP0510085A1 (de) 1992-10-28
WO1991010746A1 (en) 1991-07-25
EP0510085B1 (de) 1999-09-08
IE910081A1 (en) 1991-07-17
EP0510085A4 (en) 1993-03-24
CA2070632A1 (en) 1991-07-11
PT96458B (pt) 1998-06-30
JPH05503422A (ja) 1993-06-10
PT96458A (pt) 1991-10-31
JP2001095590A (ja) 2001-04-10
DE69131596D1 (de) 1999-10-14
ATE184321T1 (de) 1999-09-15

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