DE69128077D1 - Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen - Google Patents

Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen

Info

Publication number
DE69128077D1
DE69128077D1 DE69128077T DE69128077T DE69128077D1 DE 69128077 D1 DE69128077 D1 DE 69128077D1 DE 69128077 T DE69128077 T DE 69128077T DE 69128077 T DE69128077 T DE 69128077T DE 69128077 D1 DE69128077 D1 DE 69128077D1
Authority
DE
Germany
Prior art keywords
rns
dna
nucleic acid
dependent
replicases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69128077T
Other languages
English (en)
Other versions
DE69128077T2 (de
Inventor
Randall Dimond
Steven Ekenberg
James Hartnett
Geoffrey Hudson
Christopher Jones
Mark Maffitt
Richard Martinelli
Leopoldo Mendoza
Katherine Miller
John Monahan
Edward Pahuski
James Schumm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of DE69128077D1 publication Critical patent/DE69128077D1/de
Publication of DE69128077T2 publication Critical patent/DE69128077T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69128077T 1990-12-31 1991-12-31 Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen Expired - Fee Related DE69128077T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US63850890A 1990-12-31 1990-12-31
PCT/US1991/009776 WO1992012261A1 (en) 1990-12-31 1991-12-31 Nucleic acid amplification with dna-dependent rna polymerase activity of rna replicases

Publications (2)

Publication Number Publication Date
DE69128077D1 true DE69128077D1 (de) 1997-12-04
DE69128077T2 DE69128077T2 (de) 1998-02-26

Family

ID=24560333

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69128077T Expired - Fee Related DE69128077T2 (de) 1990-12-31 1991-12-31 Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen

Country Status (11)

Country Link
US (3) US6090589A (de)
EP (1) EP0519053B1 (de)
JP (1) JP3183510B2 (de)
KR (1) KR100231383B1 (de)
AT (1) ATE159763T1 (de)
AU (2) AU9165091A (de)
CA (1) CA2076750C (de)
DE (1) DE69128077T2 (de)
DK (1) DK0519053T3 (de)
ES (1) ES2108748T3 (de)
WO (1) WO1992012261A1 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0648281A4 (de) * 1991-09-10 1997-06-04 Jack D Love Zielgerichte amplifikation von dna und rna und signalamplifikation.
AU6027794A (en) * 1993-01-15 1994-08-15 Public Health Research Institute Of The City Of New York, Inc., The Diagnostic assays and kits for rna using rna binary probes and an rna-directed rna ligase
EP0682716A4 (de) * 1993-01-15 1999-10-27 New York Health Res Inst Rna-assays mittels binären rna proben und ribozym-ligase.
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US6593086B2 (en) 1996-05-20 2003-07-15 Mount Sinai School Of Medicine Of New York University Nucleic acid amplification methods
USRE38442E1 (en) * 1994-06-22 2004-02-24 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
FR2723591B1 (fr) * 1994-08-10 1996-09-20 Cis Bio Int Methode d'amplification et/ou de detection d'une sequence d'acide nucleique, reactif de detection et ses applications.
US5753787A (en) * 1995-04-10 1998-05-19 Yale University Nucleic acids encoding ancylostoma secreted protein
US5837466A (en) * 1996-12-16 1998-11-17 Vysis, Inc. Devices and methods for detecting nucleic acid analytes in samples
US6001570A (en) * 1997-02-18 1999-12-14 Invitro Diagnostics, Inc. Compositions, methods, kits and apparatus for determining the presence or absence of target molecules
US6108582A (en) * 1998-07-02 2000-08-22 Intermedics Inc. Cardiac pacemaker lead with extendable/retractable fixation
WO2001059162A2 (en) * 2000-02-08 2001-08-16 Bayer Corporation METHOD FOR NUCLEIC ACID DETECTION VIA Qβ REPLICASE
US6562575B1 (en) 2000-06-26 2003-05-13 Epicentre Technologies Corporation Analyte-specific assays based on formation of a replicase substrate
US6949340B2 (en) 2001-03-28 2005-09-27 Creative Mines Llc Optical phase modulator
GB0111275D0 (en) * 2001-05-09 2001-06-27 Secr Defence Analytical method and kit
US10539561B1 (en) * 2001-08-30 2020-01-21 Customarray, Inc. Enzyme-amplified redox microarray detection process
DE10143106C1 (de) * 2001-09-03 2002-10-10 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
US20120077196A9 (en) * 2001-09-03 2012-03-29 Guido Krupp Universal method for selective amplification of mRNAs
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US7238477B2 (en) * 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US7476501B2 (en) * 2002-03-26 2009-01-13 Intel Corporation Methods and device for DNA sequencing using surface enhanced raman scattering (SERS)
US20040110208A1 (en) * 2002-03-26 2004-06-10 Selena Chan Methods and device for DNA sequencing using surface enhanced Raman scattering (SERS)
US20030215816A1 (en) * 2002-05-20 2003-11-20 Narayan Sundararajan Method for sequencing nucleic acids by observing the uptake of nucleotides modified with bulky groups
DE10224200C1 (de) * 2002-05-31 2003-08-21 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
DE10240868A1 (de) * 2002-09-04 2004-03-18 Artus Gesellschaft für molekularbiologische Diagnostik und Entwicklung mbH Verbesserte Verfahren zur Synthese von Nukleinsäuren
GB0230238D0 (en) * 2002-12-28 2003-02-05 Fu Guoliang Combined exponential and linear amplification
US7718421B2 (en) 2003-02-05 2010-05-18 Iquum, Inc. Sample processing
WO2004092197A2 (en) * 2003-04-07 2004-10-28 The Regents Of The University Of California Amyloid-specific peptides and uses thereof
US20060199194A1 (en) * 2003-11-10 2006-09-07 Q-Rna, Inc. Methods of detection using immuno-Q-Amp technology
US20050214805A1 (en) * 2003-11-10 2005-09-29 Q-Rna, Inc. Methods of detection employing immuno-Q-Amp technology
GB0517005D0 (en) * 2005-08-19 2005-09-28 Enigma Diagnostics Ltd Analytical method and kit
US20070065834A1 (en) * 2005-09-19 2007-03-22 Hillis William D Method and sequences for determinate nucleic acid hybridization
US20080003564A1 (en) * 2006-02-14 2008-01-03 Iquum, Inc. Sample processing
WO2012038448A1 (en) * 2010-09-21 2012-03-29 Riboxx Gmbh Method for synthesizing rna using dna template
JP5951755B2 (ja) * 2011-05-04 2016-07-13 エイチティージー モレキュラー ダイアグノスティクス， インコーポレイテッド 定量的ヌクレアーゼプロテクションアッセイ（ｑＮＰＡ）法および定量的ヌクレアーゼプロテクション配列決定（ｑＮＰＳ）法の改善

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4786600A (en) * 1984-05-25 1988-11-22 The Trustees Of Columbia University In The City Of New York Autocatalytic replication of recombinant RNA
CA1339981C (en) * 1984-05-25 1998-08-04 Fred R. Kramer Replicatable hybridizable recombinant rna probes and methods of using same
US4957858A (en) * 1986-04-16 1990-09-18 The Salk Instute For Biological Studies Replicative RNA reporter systems
ES8706823A1 (es) 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
CA1339991C (en) 1988-09-08 1998-08-11 The Trustees Of Columbia University Replicative rna-based amplification/detection system
WO1990002819A1 (en) * 1988-09-08 1990-03-22 The Salk Institute For Biological Studies Replicative rna-based amplification detection systems
ZA899593B (en) * 1988-12-16 1990-09-26 Siska Diagnostics Inc Self-sustained,sequence replication system
US5112734A (en) * 1989-05-26 1992-05-12 Gene-Trak Systems Target-dependent synthesis of an artificial gene for the synthesis of a replicatable rna
US5480784A (en) * 1989-07-11 1996-01-02 Gen-Probe Incorporated Nucleic acid sequence amplification methods
CA2040503A1 (en) * 1990-04-25 1991-10-26 James E. Stefano Selective amplification system using q beta replicase

Also Published As

Publication number Publication date
EP0519053A4 (en) 1993-03-31
US6677140B2 (en) 2004-01-13
DE69128077T2 (de) 1998-02-26
US6090589A (en) 2000-07-18
CA2076750A1 (en) 1992-07-01
DK0519053T3 (da) 1998-07-20
ATE159763T1 (de) 1997-11-15
EP0519053B1 (de) 1997-10-29
AU694199B2 (en) 1998-07-16
KR100231383B1 (ko) 1999-11-15
CA2076750C (en) 2003-07-08
JP3183510B2 (ja) 2001-07-09
AU2022295A (en) 1995-09-21
WO1992012261A1 (en) 1992-07-23
EP0519053A1 (de) 1992-12-23
US20020192677A1 (en) 2002-12-19
JPH05505111A (ja) 1993-08-05
US6369207B1 (en) 2002-04-09
ES2108748T3 (es) 1998-01-01
AU9165091A (en) 1992-08-17

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Legal Events

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8339 Ceased/non-payment of the annual fee