WO1990009457A3 - Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them - Google Patents

Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them Download PDF

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Publication number
WO1990009457A3
WO1990009457A3 PCT/US1990/000866 US9000866W WO9009457A3 WO 1990009457 A3 WO1990009457 A3 WO 1990009457A3 US 9000866 W US9000866 W US 9000866W WO 9009457 A3 WO9009457 A3 WO 9009457A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
primer pairs
oligonucleotide primer
employ
gene amplification
Prior art date
Application number
PCT/US1990/000866
Other languages
French (fr)
Other versions
WO1990009457A2 (en
Inventor
Mark P Pasek
Original Assignee
Biogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Inc filed Critical Biogen Inc
Publication of WO1990009457A2 publication Critical patent/WO1990009457A2/en
Publication of WO1990009457A3 publication Critical patent/WO1990009457A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

The present invention relates to methods for amplifying isolated nucleic acid sequences, particularly DNA sequences, whose nucleotide sequences are unknown. The invention also relates to oligonucleotide primer pairs that may be employed in the amplification methods of the present invention.
PCT/US1990/000866 1989-02-14 1990-02-14 Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them WO1990009457A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31076389A 1989-02-14 1989-02-14
US310,763 1989-02-14

Publications (2)

Publication Number Publication Date
WO1990009457A2 WO1990009457A2 (en) 1990-08-23
WO1990009457A3 true WO1990009457A3 (en) 1990-10-04

Family

ID=23203992

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/000866 WO1990009457A2 (en) 1989-02-14 1990-02-14 Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them

Country Status (4)

Country Link
EP (1) EP0458909A1 (en)
AU (1) AU5404890A (en)
CA (1) CA2046919A1 (en)
WO (1) WO1990009457A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE68927373T2 (en) * 1988-06-24 1997-03-20 Amgen Inc METHOD AND MEANS FOR DETECTING NUCLEIC ACID SEQUENCES
GB8818020D0 (en) * 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
EP0545459A1 (en) * 1989-01-31 1993-06-09 The Regents Of The University Of California Amplification of flow-sorted chromosomal DNA
WO1990008821A1 (en) * 1989-01-31 1990-08-09 University Of Miami Microdissection and amplification of chromosomal dna
HUT69140A (en) * 1990-08-10 1995-08-28 Chiron Corp Nanbv diagnostics: polynucleotides useful for screening for hepatitis c virus
FR2678639B1 (en) * 1991-07-03 1993-09-17 Rhone Poulenc Rorer Sa PROCESS FOR CLONING NUCLEIC ACIDS.
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
US5710000A (en) 1994-09-16 1998-01-20 Affymetrix, Inc. Capturing sequences adjacent to Type-IIs restriction sites for genomic library mapping
JP2002517981A (en) * 1998-01-13 2002-06-25 ビオヒプ テヒノロギース ゲゼルシャフト ミット ベシュレンクテル ハフツング Methods for detecting nucleic acid sequences
DE10108626A1 (en) * 2001-02-22 2002-09-05 Sahin Ugur Procedure for the identification of biologically active structures of microbial pathogens

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988010315A1 (en) * 1987-06-19 1988-12-29 Siska Diagnostics, Inc. Transcription-based nucleic acid amplification/detection systems
WO1989012695A1 (en) * 1988-06-17 1989-12-28 Genelabs Incorporated Dna amplification and subtraction techniques
WO1990001065A1 (en) * 1988-07-26 1990-02-08 Genelabs Incorporated Rna and dna amplification techniques
WO1990001064A1 (en) * 1988-07-26 1990-02-08 Genelabs Incorporated Sequence-specific amplification techniques
EP0201184B1 (en) * 1985-03-28 1992-12-16 F. Hoffmann-La Roche Ag Process for amplifying nucleic acid sequences

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0201184B1 (en) * 1985-03-28 1992-12-16 F. Hoffmann-La Roche Ag Process for amplifying nucleic acid sequences
WO1988010315A1 (en) * 1987-06-19 1988-12-29 Siska Diagnostics, Inc. Transcription-based nucleic acid amplification/detection systems
WO1989012695A1 (en) * 1988-06-17 1989-12-28 Genelabs Incorporated Dna amplification and subtraction techniques
WO1990001065A1 (en) * 1988-07-26 1990-02-08 Genelabs Incorporated Rna and dna amplification techniques
WO1990001064A1 (en) * 1988-07-26 1990-02-08 Genelabs Incorporated Sequence-specific amplification techniques

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Gene, Volume 34, No. 2/3, 1985, Elsevier Science Publishers, (Amsterdam, NL), C. COLECLOUGH et al.: "Use of Primer-Restriction-End Adapters in a Novel cDNA Cloning Strategy", pages 305-314 *
Nucleic Acids Research, Volume 14, No. 4, 1986, IRL Press Limited, (Oxford, GB), U. KRAWINKEL et al.: "Rapid Synthesis of cDNA for Cloning into Lambda Vectors", page 1913 *
Nucleic Acids Research, Volume 17, No. 8, April 1989, IRL Press, A. BELYAVSKY et al.: "PCR-Based cDNA Library Construction: General cDNA Libraries at the Level of a Few Cells", pages 2919-2932 see the whole article *
Proc. Natl. Acad. Sci. USA, Volume 85, December 1988, M.A. INNIS et al.: "DNA Sequencing with Thermus Aquaticus DNA Polymerase and Direct Sequencing of Polymerase Chain Reaction-Amplified DNA", pages 9436-9440 *
Science, Volume 243, 13 January 1989, E.Y. LOH et al.: "Polymerase Chain Reaction with Single-Sided Specificity: Analysis of T Cell Receptor & Chain", pages 217-218 *

Also Published As

Publication number Publication date
CA2046919A1 (en) 1990-08-15
EP0458909A1 (en) 1991-12-04
WO1990009457A2 (en) 1990-08-23
AU5404890A (en) 1990-09-05

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