DE60327775D1 - Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen - Google Patents

Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen

Info

Publication number
DE60327775D1
DE60327775D1 DE60327775T DE60327775T DE60327775D1 DE 60327775 D1 DE60327775 D1 DE 60327775D1 DE 60327775 T DE60327775 T DE 60327775T DE 60327775 T DE60327775 T DE 60327775T DE 60327775 D1 DE60327775 D1 DE 60327775D1
Authority
DE
Germany
Prior art keywords
nucleic acid
isolation
molecules
rna
nucleotide sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60327775T
Other languages
English (en)
Inventor
Sakari Kauppinen
Nana Jacobsen
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Exiqon AS
Original Assignee
Exiqon AS
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Filing date
Publication date
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Application granted granted Critical
Publication of DE60327775D1 publication Critical patent/DE60327775D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE60327775T 2002-06-24 2003-06-20 Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen Expired - Lifetime DE60327775D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39092802P 2002-06-24 2002-06-24
PCT/IB2003/006354 WO2004020575A2 (en) 2002-06-24 2003-06-20 Methods and systems for detection and isolation of a nucleotide sequence

Publications (1)

Publication Number Publication Date
DE60327775D1 true DE60327775D1 (de) 2009-07-09

Family

ID=31978221

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60327775T Expired - Lifetime DE60327775D1 (de) 2002-06-24 2003-06-20 Methoden und systeme zur detektion und isolation von nucleinsäuresequenzen

Country Status (6)

Country Link
US (2) US20050053942A1 (de)
EP (1) EP1527175B1 (de)
AT (1) ATE432347T1 (de)
AU (1) AU2003288474A1 (de)
DE (1) DE60327775D1 (de)
WO (1) WO2004020575A2 (de)

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US20120237451A1 (en) * 2009-09-18 2012-09-20 Antony Kuang-Shih Chen Novel molecular beacons
US8278049B2 (en) * 2010-04-26 2012-10-02 Ann & Robert H. Lurie Children's Hospital of Chicago Selective enrichment of CpG islands
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DE12722942T1 (de) 2011-03-31 2021-09-30 Modernatx, Inc. Freisetzung und formulierung von manipulierten nukleinsäuren
WO2013028902A2 (en) * 2011-08-23 2013-02-28 University Of Medicine And Dentistry Of New Jersey Methods of isolating rna and mapping of polyadenylation isoforms
US9556494B2 (en) 2011-11-23 2017-01-31 Cornell University Methods for diagnosing human immunodeficiency virus infections
US9725770B2 (en) * 2012-03-06 2017-08-08 The Regents Of The University Of California Methods and compositions for identification of source of microbial contamination in a sample
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
EP2834259A4 (de) 2012-04-02 2016-08-24 Moderna Therapeutics Inc Modifizierte polynukleotide
US10086093B2 (en) 2013-02-28 2018-10-02 The General Hospital Corporation miRNA profiling compositions and methods of use
AU2014239264A1 (en) * 2013-03-14 2015-08-27 Shire Human Genetic Therapies, Inc. Quantitative assessment for cap efficiency of messenger RNA
EP3578663A1 (de) 2013-03-15 2019-12-11 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
WO2014152031A1 (en) * 2013-03-15 2014-09-25 Moderna Therapeutics, Inc. Ribonucleic acid purification
EP2983804A4 (de) 2013-03-15 2017-03-01 Moderna Therapeutics, Inc. Ionenaustauschreinigung von mrna
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
CN103308373A (zh) * 2013-06-05 2013-09-18 南昌大学 磁珠分离大肠杆菌o157的方法
JP7019233B2 (ja) 2013-07-11 2022-02-15 モデルナティエックス インコーポレイテッド ＣＲＩＳＰＲ関連タンパク質をコードする合成ポリヌクレオチドおよび合成ｓｇＲＮＡを含む組成物ならびに使用方法
EP3052511A4 (de) 2013-10-02 2017-05-31 Moderna Therapeutics, Inc. Polynukleotidmoleküle und verwendungen davon
US10286086B2 (en) 2014-06-19 2019-05-14 Modernatx, Inc. Alternative nucleic acid molecules and uses thereof
US10407683B2 (en) 2014-07-16 2019-09-10 Modernatx, Inc. Circular polynucleotides
US10093967B2 (en) * 2014-08-12 2018-10-09 The Regents Of The University Of Michigan Detection of nucleic acids
ES2879964T3 (es) * 2014-12-12 2021-11-23 Exact Sciences Dev Co Llc Composiciones y métodos para realizar ensayos de detección de metilación
US11434486B2 (en) 2015-09-17 2022-09-06 Modernatx, Inc. Polynucleotides containing a morpholino linker
KR102648647B1 (ko) * 2015-09-22 2024-03-18 비오까르띠 엔브이 짧은 호모폴리머릭 반복서열의 개선된 검출법
WO2017068087A1 (en) * 2015-10-22 2017-04-27 Roche Innovation Center Copenhagen A/S Oligonucleotide detection method
CN108519481B (zh) * 2018-03-08 2020-10-16 捷和泰（北京）生物科技有限公司 一种改善核心抗体磁微粒化学发光免疫分析精密度的方法
WO2020073018A2 (en) * 2018-10-04 2020-04-09 First Light Diagnostics, Inc. Test cartridges
KR20230054790A (ko) * 2019-07-16 2023-04-25 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
US20220325322A1 (en) * 2019-08-02 2022-10-13 Vladimir I. Slepnev Sequence specific nucleic acid enrichment methods and uses thereof
CN114107446A (zh) * 2021-12-16 2022-03-01 福建和瑞基因科技有限公司 一种核酸的检测试剂盒及其检测方法

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Also Published As

Publication number Publication date
US20050053942A1 (en) 2005-03-10
EP1527175A2 (de) 2005-05-04
WO2004020575A2 (en) 2004-03-11
WO2004020575A3 (en) 2004-12-23
EP1527175B1 (de) 2009-05-27
ATE432347T1 (de) 2009-06-15
AU2003288474A8 (en) 2004-03-19
AU2003288474A1 (en) 2004-03-19
US20080096191A1 (en) 2008-04-24

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