DE60239456D1 - Amplifizierungsverfahren - Google Patents
AmplifizierungsverfahrenInfo
- Publication number
- DE60239456D1 DE60239456D1 DE60239456T DE60239456T DE60239456D1 DE 60239456 D1 DE60239456 D1 DE 60239456D1 DE 60239456 T DE60239456 T DE 60239456T DE 60239456 T DE60239456 T DE 60239456T DE 60239456 D1 DE60239456 D1 DE 60239456D1
- Authority
- DE
- Germany
- Prior art keywords
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003321 amplification Effects 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/50—Other enzymatic activities
- C12Q2521/525—Phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
- C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
- C12Q2549/101—Hot start
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GBGB0110501.4A GB0110501D0 (en) | 2001-04-30 | 2001-04-30 | Amplification process |
PCT/GB2002/001861 WO2002088387A2 (en) | 2001-04-30 | 2002-04-22 | Amplification process |
Publications (1)
Publication Number | Publication Date |
---|---|
DE60239456D1 true DE60239456D1 (de) | 2011-04-28 |
Family
ID=9913697
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60239456T Expired - Lifetime DE60239456D1 (de) | 2001-04-30 | 2002-04-22 | Amplifizierungsverfahren |
Country Status (14)
Country | Link |
---|---|
US (2) | US6951744B2 (de) |
EP (1) | EP1390532B1 (de) |
JP (1) | JP4177118B2 (de) |
KR (2) | KR20100005245A (de) |
CN (1) | CN1318604C (de) |
AR (1) | AR035238A1 (de) |
AT (1) | ATE502118T1 (de) |
AU (1) | AU2002249444B2 (de) |
CA (1) | CA2445864C (de) |
DE (1) | DE60239456D1 (de) |
GB (2) | GB0110501D0 (de) |
NZ (1) | NZ528919A (de) |
TW (1) | TWI323284B (de) |
WO (1) | WO2002088387A2 (de) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0112868D0 (en) | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
CN1500887A (zh) * | 2002-10-01 | 2004-06-02 | 松下电器产业株式会社 | 引物伸长反应检测方法、碱基种类判别方法及其装置 |
US20060051796A1 (en) * | 2004-09-09 | 2006-03-09 | Inga Boell | Real time PCR with the addition of pyrophosphatase |
EP1634965B1 (de) | 2004-09-09 | 2010-01-20 | Roche Diagnostics GmbH | Echtzeit-PCR unter Zusatz von Pyrophosphatase |
GB0502010D0 (en) * | 2005-02-01 | 2005-03-09 | Enigma Diagnostics Ltd | Biochemical reagents and their uses |
WO2007139723A1 (en) | 2006-06-01 | 2007-12-06 | Trilink Biotechnologies | Chemicallymodified oligonucleotide primers for nucleic acid amplification |
KR101098764B1 (ko) | 2007-10-29 | 2011-12-26 | (주)바이오니아 | 안정화된 핫스타트 pcr용 건조 조성물 |
CA2725239C (en) | 2008-05-27 | 2019-02-26 | Trilink Biotechnologies | Chemically modified nucleoside 5'-triphosphates for thermally initiated amplification of nucleic acid |
EP2566959A1 (de) * | 2010-04-30 | 2013-03-13 | Roche Diagniostics GmbH | System und verfahren zur reinigung und verwendung anorganischer pyrophosphatase aus aquifex aeolicus |
US20110312694A1 (en) * | 2010-06-17 | 2011-12-22 | Geneasys Pty Ltd | Microfluidic device with delay-triggered photodetection of fluorescent probe-target hybrid |
KR101870311B1 (ko) * | 2012-03-09 | 2018-06-25 | (주)바이오니아 | 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물 |
CN110343673B (zh) | 2012-06-14 | 2024-04-05 | 生命技术公司 | 用于聚合酶链式反应(pcr)的新型组合物、方法和试剂盒 |
GB201301457D0 (en) | 2013-01-28 | 2013-03-13 | Fluorogenics Ltd | Freeze-dried composition |
JP6467829B2 (ja) * | 2014-09-03 | 2019-02-13 | 東洋紡株式会社 | 改良されたrt−pcr反応 |
CN104862288B (zh) * | 2015-05-08 | 2019-10-29 | 苏州大学 | 一种新型温度响应性无机焦磷酸酶偶联物的合成及其在增强聚合酶链式反应中的应用 |
LT3402880T (lt) | 2016-01-15 | 2024-03-12 | Thermo Fisher Scientific Baltics Uab | Termofiliniai dnr polimerazės mutantai |
EP3645710A1 (de) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophile dna-polymerase-mutanten |
JP7234598B2 (ja) * | 2018-01-26 | 2023-03-08 | 東ソー株式会社 | 核酸増幅試薬および当該試薬を用いた核酸増幅制御方法 |
CN113481180A (zh) * | 2021-07-05 | 2021-10-08 | 吉林大学 | 碱性嗜热无机焦磷酸酶及其在增强聚合酶链式反应和合成udp-半乳糖反应中的应用 |
Family Cites Families (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4868103A (en) | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
CH670709A5 (de) * | 1986-04-24 | 1989-06-30 | Univ Moskovsk | |
US5498523A (en) * | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
WO1990012111A1 (en) * | 1989-04-12 | 1990-10-18 | President And Fellows Of Harvard College | Improved primer extension reactions |
KR100236506B1 (ko) | 1990-11-29 | 2000-01-15 | 퍼킨-엘머시터스인스트루먼츠 | 폴리머라제 연쇄 반응 수행 장치 |
FI923911A (fi) * | 1992-09-01 | 1994-03-02 | Vsevolod Kiselev | DNA-molekylers in vitro-syntes |
US5565339A (en) | 1992-10-08 | 1996-10-15 | Hoffmann-La Roche Inc. | Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents |
US5491063A (en) | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
US5773258A (en) | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
US5665551A (en) * | 1995-09-13 | 1997-09-09 | Roche Molecular Systems, Inc. | Purified nucleic acid encoding a thermostable pyrophosphatase |
US6291164B1 (en) * | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
GB9716052D0 (en) | 1996-12-06 | 1997-10-01 | Secr Defence | Reaction vessels |
GB9725197D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
GB9725237D0 (en) | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
GB9803382D0 (en) | 1998-02-19 | 1998-04-15 | Secr Defence | Detection system |
US6183998B1 (en) | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
GB9812768D0 (en) | 1998-06-13 | 1998-08-12 | Zeneca Ltd | Methods |
KR100292883B1 (ko) * | 1999-02-09 | 2001-06-15 | 박한오 | 피로포스페이트와 피로포스파타제를 포함하는 핫 스타트 pcr 반응혼합물 |
EP2112233B1 (de) | 2000-02-23 | 2013-10-23 | City of Hope | Pyrophosphorolyse-aktivierte Polymerisierung: Anwendung auf die allelespezifische Verstärkung und Nukleinsäuresequenzbestimmung |
KR20050005626A (ko) * | 2003-07-07 | 2005-01-14 | 주식회사 포스코 | 코크스 오븐에서의 장입탄 밀도 향상방법 |
-
2001
- 2001-04-30 GB GBGB0110501.4A patent/GB0110501D0/en not_active Ceased
-
2002
- 2002-03-28 TW TW091106126A patent/TWI323284B/zh not_active IP Right Cessation
- 2002-04-22 GB GB0209052A patent/GB2377991B/en not_active Expired - Fee Related
- 2002-04-22 KR KR1020097025792A patent/KR20100005245A/ko not_active Application Discontinuation
- 2002-04-22 EP EP02718374A patent/EP1390532B1/de not_active Expired - Lifetime
- 2002-04-22 WO PCT/GB2002/001861 patent/WO2002088387A2/en active Application Filing
- 2002-04-22 NZ NZ528919A patent/NZ528919A/en unknown
- 2002-04-22 AT AT02718374T patent/ATE502118T1/de not_active IP Right Cessation
- 2002-04-22 AU AU2002249444A patent/AU2002249444B2/en not_active Ceased
- 2002-04-22 CN CNB028132726A patent/CN1318604C/zh not_active Expired - Fee Related
- 2002-04-22 DE DE60239456T patent/DE60239456D1/de not_active Expired - Lifetime
- 2002-04-22 KR KR1020037014191A patent/KR101039563B1/ko not_active IP Right Cessation
- 2002-04-22 CA CA2445864A patent/CA2445864C/en not_active Expired - Fee Related
- 2002-04-22 JP JP2002585667A patent/JP4177118B2/ja not_active Expired - Fee Related
- 2002-04-29 AR ARP020101570A patent/AR035238A1/es unknown
- 2002-04-30 US US10/135,807 patent/US6951744B2/en not_active Expired - Fee Related
-
2005
- 2005-08-17 US US11/205,667 patent/US7449312B2/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
TWI323284B (en) | 2010-04-11 |
ATE502118T1 (de) | 2011-04-15 |
AU2002249444B2 (en) | 2007-07-12 |
GB2377991B (en) | 2004-01-28 |
KR101039563B1 (ko) | 2011-06-09 |
CA2445864C (en) | 2011-08-02 |
US20030049655A1 (en) | 2003-03-13 |
KR20040015216A (ko) | 2004-02-18 |
GB0110501D0 (en) | 2001-06-20 |
AR035238A1 (es) | 2004-05-05 |
EP1390532B1 (de) | 2011-03-16 |
CA2445864A1 (en) | 2002-11-07 |
WO2002088387A3 (en) | 2003-12-11 |
KR20100005245A (ko) | 2010-01-14 |
US6951744B2 (en) | 2005-10-04 |
US7449312B2 (en) | 2008-11-11 |
GB0209052D0 (en) | 2002-05-29 |
JP2004524853A (ja) | 2004-08-19 |
CN1318604C (zh) | 2007-05-30 |
NZ528919A (en) | 2005-07-29 |
WO2002088387A2 (en) | 2002-11-07 |
GB2377991A (en) | 2003-01-29 |
CN1522305A (zh) | 2004-08-18 |
JP4177118B2 (ja) | 2008-11-05 |
EP1390532A2 (de) | 2004-02-25 |
US20060057617A1 (en) | 2006-03-16 |
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