DE60227669D1 - Verfahren zur herstellung von polynukleotidfragmenten zur verwendung im shuffling - Google Patents

Verfahren zur herstellung von polynukleotidfragmenten zur verwendung im shuffling

Info

Publication number
DE60227669D1
DE60227669D1 DE60227669T DE60227669T DE60227669D1 DE 60227669 D1 DE60227669 D1 DE 60227669D1 DE 60227669 T DE60227669 T DE 60227669T DE 60227669 T DE60227669 T DE 60227669T DE 60227669 D1 DE60227669 D1 DE 60227669D1
Authority
DE
Germany
Prior art keywords
shuffling
polynucleotide fragments
producing polynucleotide
fragments
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60227669T
Other languages
English (en)
Inventor
Daniel Dupret
Fabrice Lefevre
Laurent Fourage
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Proteus SA
Original Assignee
Proteus SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Proteus SA filed Critical Proteus SA
Application granted granted Critical
Publication of DE60227669D1 publication Critical patent/DE60227669D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE60227669T 2001-05-17 2002-05-16 Verfahren zur herstellung von polynukleotidfragmenten zur verwendung im shuffling Expired - Lifetime DE60227669D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29118401P 2001-05-17 2001-05-17
PCT/IB2002/002769 WO2002092815A2 (en) 2001-05-17 2002-05-16 Method of preparing polynucleotide fragments for use in shuffling, and shuffling of same

Publications (1)

Publication Number Publication Date
DE60227669D1 true DE60227669D1 (de) 2008-08-28

Family

ID=23119242

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60227669T Expired - Lifetime DE60227669D1 (de) 2001-05-17 2002-05-16 Verfahren zur herstellung von polynukleotidfragmenten zur verwendung im shuffling

Country Status (8)

Country Link
US (1) US7303897B2 (de)
EP (1) EP1404830B1 (de)
AT (1) ATE401398T1 (de)
AU (1) AU2002342955A1 (de)
DE (1) DE60227669D1 (de)
DK (1) DK1404830T3 (de)
ES (1) ES2310597T3 (de)
WO (1) WO2002092815A2 (de)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5556750A (en) * 1989-05-12 1996-09-17 Duke University Methods and kits for fractionating a population of DNA molecules based on the presence or absence of a base-pair mismatch utilizing mismatch repair systems
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO1997021537A1 (en) 1995-12-11 1997-06-19 Shell Internationale Research Maatschappij B.V. Cross-linked polyketones
KR20010032861A (ko) * 1997-12-08 2001-04-25 캘리포니아 인스티튜트 오브 테크놀로지 폴리뉴클레오티드 및 폴리펩티드 서열을 형성하는 방법

Also Published As

Publication number Publication date
AU2002342955A1 (en) 2002-11-25
EP1404830A2 (de) 2004-04-07
DK1404830T3 (da) 2008-11-10
ATE401398T1 (de) 2008-08-15
EP1404830B1 (de) 2008-07-16
WO2002092815A3 (en) 2003-06-05
WO2002092815A2 (en) 2002-11-21
US20040214197A1 (en) 2004-10-28
US7303897B2 (en) 2007-12-04
ES2310597T3 (es) 2009-01-16

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Legal Events

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