ATE516351T1 - Verfahren zur molekularen evolution der proteinfunktion in vitro - Google Patents

Verfahren zur molekularen evolution der proteinfunktion in vitro

Info

Publication number
ATE516351T1
ATE516351T1 AT06808581T AT06808581T ATE516351T1 AT E516351 T1 ATE516351 T1 AT E516351T1 AT 06808581 T AT06808581 T AT 06808581T AT 06808581 T AT06808581 T AT 06808581T AT E516351 T1 ATE516351 T1 AT E516351T1
Authority
AT
Austria
Prior art keywords
polynucleotide
sequence
parent
population
fragments
Prior art date
Application number
AT06808581T
Other languages
English (en)
Inventor
Karin Haraldsson
Marie Karlsson
Ann-Christin Hager
Christina Furebring
Fredrik Karlsson
Peter Ellmark
Original Assignee
Alligator Bioscience Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alligator Bioscience Ab filed Critical Alligator Bioscience Ab
Application granted granted Critical
Publication of ATE516351T1 publication Critical patent/ATE516351T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT06808581T 2005-11-19 2006-11-17 Verfahren zur molekularen evolution der proteinfunktion in vitro ATE516351T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0523582A GB2432366B (en) 2005-11-19 2005-11-19 A method for in vitro molecular evolution of protein function
PCT/GB2006/004294 WO2007057682A1 (en) 2005-11-19 2006-11-17 A method for in vitro molecular evolution of protein function

Publications (1)

Publication Number Publication Date
ATE516351T1 true ATE516351T1 (de) 2011-07-15

Family

ID=35580344

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06808581T ATE516351T1 (de) 2005-11-19 2006-11-17 Verfahren zur molekularen evolution der proteinfunktion in vitro

Country Status (12)

Country Link
US (1) US20090203572A1 (de)
EP (1) EP1948794B1 (de)
JP (1) JP5349051B2 (de)
CN (1) CN101351553B (de)
AT (1) ATE516351T1 (de)
AU (1) AU2006314310B2 (de)
CA (1) CA2630183C (de)
DK (1) DK1948794T3 (de)
ES (1) ES2395249T3 (de)
GB (1) GB2432366B (de)
PT (1) PT1948794E (de)
WO (1) WO2007057682A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
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GB0708376D0 (en) 2007-05-01 2007-06-06 Alligator Bioscience Ab Novel polypeptides and uses thereof
GB0905790D0 (en) 2009-04-03 2009-05-20 Alligator Bioscience Ab Novel polypeptides and use thereof
GB0905503D0 (en) * 2009-03-31 2009-05-13 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
GB201019086D0 (en) 2010-11-11 2010-12-29 Imp Innovations Ltd Bacterial methods
GB201311487D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Bispecific molecules
GB201311475D0 (en) 2013-06-27 2013-08-14 Alligator Bioscience Ab Polypeptides

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Also Published As

Publication number Publication date
US20090203572A1 (en) 2009-08-13
EP1948794A1 (de) 2008-07-30
DK1948794T3 (da) 2011-10-24
WO2007057682A1 (en) 2007-05-24
CA2630183C (en) 2014-07-15
GB2432366B (en) 2007-11-21
JP2009515546A (ja) 2009-04-16
AU2006314310B2 (en) 2012-04-26
CA2630183A1 (en) 2007-05-24
AU2006314310A1 (en) 2007-05-24
CN101351553B (zh) 2012-09-05
PT1948794E (pt) 2011-09-12
EP1948794B1 (de) 2011-07-13
JP5349051B2 (ja) 2013-11-20
ES2395249T3 (es) 2013-02-11
GB2432366A (en) 2007-05-23
GB0523582D0 (en) 2005-12-28
CN101351553A (zh) 2009-01-21

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