DE602005027144D1 - Vorrichtung und Verfahren zur PCR-Amplifizierungsreaktion - Google Patents
Vorrichtung und Verfahren zur PCR-AmplifizierungsreaktionInfo
- Publication number
- DE602005027144D1 DE602005027144D1 DE602005027144T DE602005027144T DE602005027144D1 DE 602005027144 D1 DE602005027144 D1 DE 602005027144D1 DE 602005027144 T DE602005027144 T DE 602005027144T DE 602005027144 T DE602005027144 T DE 602005027144T DE 602005027144 D1 DE602005027144 D1 DE 602005027144D1
- Authority
- DE
- Germany
- Prior art keywords
- pcr amplification
- amplification reaction
- reaction
- pcr
- amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004130041A JP4592060B2 (ja) | 2004-04-26 | 2004-04-26 | Pcr増幅反応装置、ならびに、該装置を利用するpcr増幅反応方法 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602005027144D1 true DE602005027144D1 (de) | 2011-05-12 |
Family
ID=34941021
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE602005027144T Active DE602005027144D1 (de) | 2004-04-26 | 2005-04-26 | Vorrichtung und Verfahren zur PCR-Amplifizierungsreaktion |
Country Status (5)
Country | Link |
---|---|
US (1) | US7569366B2 (de) |
EP (1) | EP1591543B1 (de) |
JP (1) | JP4592060B2 (de) |
CN (1) | CN100532567C (de) |
DE (1) | DE602005027144D1 (de) |
Families Citing this family (53)
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JP2004337042A (ja) * | 2003-05-14 | 2004-12-02 | Canon Inc | 核酸アレイ |
EP1713911A4 (de) * | 2003-12-25 | 2008-02-20 | Kenmoku Takashi | Sondensatz und verfahren zur identifizierung des hla-allels |
US20060289786A1 (en) * | 2005-05-04 | 2006-12-28 | Stratagene California | System and method for a pulsed light source used in fluorescence detection |
ITBO20050481A1 (it) | 2005-07-19 | 2007-01-20 | Silicon Biosystems S R L | Metodo ed apparato per la manipolazione e/o l'individuazione di particelle |
US7541314B2 (en) * | 2006-02-24 | 2009-06-02 | Combimatrix Corporation | Microarray having a base cleavable sulfonyl linker |
ITTO20060226A1 (it) | 2006-03-27 | 2007-09-28 | Silicon Biosystem S P A | Metodo ed apparato per il processamento e o l'analisi e o la selezione di particelle, in particolare particelle biologiche |
JP5187932B2 (ja) * | 2006-11-01 | 2013-04-24 | 独立行政法人科学技術振興機構 | 生体分子アッセイチップ |
JP5159098B2 (ja) * | 2006-12-01 | 2013-03-06 | キヤノン株式会社 | 複対立遺伝子のハプロタイプ決定方法 |
JP2008228585A (ja) * | 2007-03-16 | 2008-10-02 | Canon Inc | 細胞培養容器および細胞培養装置 |
JP2009060859A (ja) * | 2007-09-07 | 2009-03-26 | Sumitomo Bakelite Co Ltd | 遺伝子の検出方法 |
CN101644645A (zh) * | 2008-08-04 | 2010-02-10 | 同方威视技术股份有限公司 | 一种用于痕量检测仪器的样品解析器 |
RU2385940C1 (ru) * | 2008-10-23 | 2010-04-10 | Общество с ограниченной ответственностью "ВИНТЕЛ" | Способ определения нуклеиновых кислот методом полимеразно-цепной реакции в режиме реального времени и устройство для его осуществления |
IT1391619B1 (it) | 2008-11-04 | 2012-01-11 | Silicon Biosystems Spa | Metodo per l'individuazione, selezione e analisi di cellule tumorali |
US10895575B2 (en) | 2008-11-04 | 2021-01-19 | Menarini Silicon Biosystems S.P.A. | Method for identification, selection and analysis of tumour cells |
CN101735949B (zh) * | 2008-11-05 | 2012-10-10 | 广达电脑股份有限公司 | 变温装置 |
US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
US8795592B2 (en) | 2010-09-23 | 2014-08-05 | Analogic Corporation | Sample thermal cycling |
CN103282496B (zh) * | 2010-10-29 | 2015-04-22 | 瑞基海洋生物科技股份有限公司 | 聚合酶连锁反应的温度设定方法及装置 |
JP2012103019A (ja) * | 2010-11-08 | 2012-05-31 | Hitachi High-Technologies Corp | 反応プレートアセンブリ、反応プレート及び核酸分析装置 |
WO2012063647A1 (ja) * | 2010-11-08 | 2012-05-18 | 株式会社日立ハイテクノロジーズ | 反応プレートアセンブリ、反応プレート及び核酸分析装置 |
GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
JP6081071B2 (ja) * | 2012-03-23 | 2017-02-15 | 東芝メディカルシステムズ株式会社 | 核酸検出用デバイス |
US9745547B2 (en) * | 2011-10-10 | 2017-08-29 | Dasgip Information And Technology Gmbh | Method for controlled operation of a biotechnological apparatus and bioreactor system |
ITTO20110990A1 (it) | 2011-10-28 | 2013-04-29 | Silicon Biosystems Spa | Metodo ed apparato per l'analisi ottica di particelle a basse temperature |
ITBO20110766A1 (it) | 2011-12-28 | 2013-06-29 | Silicon Biosystems Spa | Dispositivi, apparato, kit e metodo per il trattamento di un campione biologico |
AU2013202793B2 (en) * | 2012-07-31 | 2014-09-18 | Gen-Probe Incorporated | System, method and apparatus for automated incubation |
LT3013984T (lt) | 2013-06-25 | 2023-06-12 | Prognosys Biosciences, Inc. | Biologinių taikinių erdvinių modelių mėginyje nustatymo būdai |
ES2955488T3 (es) | 2015-04-10 | 2023-12-01 | Spatial Transcriptomics Ab | Análisis multiplex de especímenes biológicos de ácidos nucleicos espacialmente distinguidos |
EP3296386B1 (de) * | 2015-05-12 | 2020-05-06 | Xiamen University | Nukleinsäureamplifikationsreaktionsgefäss zur steuerung eines flüssigkeitszirkulationsweges |
DE102016120124B8 (de) * | 2016-10-21 | 2018-08-23 | Gna Biosolutions Gmbh | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
DE102018103215B3 (de) * | 2018-02-13 | 2019-08-14 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Extraktion einer Nukleinsäure aus einer Probenflüssigkeit |
CN110819702B (zh) * | 2018-08-13 | 2023-03-21 | 上海新微技术研发中心有限公司 | 一种数字pcr***及数字pcr液滴形成方法 |
EP3894588A1 (de) | 2018-12-10 | 2021-10-20 | 10X Genomics, Inc. | Auflösung von räumlichen anordnungen unter verwendung von dekonvolution |
US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
DE102019114011B3 (de) * | 2019-05-07 | 2020-07-30 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zum Extrahieren und/oder Vervielfältigen einer Target-Nukleinsäure |
EP3976820A1 (de) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe |
EP3569717A3 (de) | 2019-08-28 | 2020-04-08 | Boehringer Ingelheim Vetmedica GmbH | Diagnostisches verfahren zur dedektion von dna und rna viren mittels polymerase kettenreaktion in einem beheizten reaktionsvolumen |
CN114885610A (zh) | 2019-12-23 | 2022-08-09 | 10X基因组学有限公司 | 使用rna模板化连接进行空间分析的方法 |
US11898205B2 (en) | 2020-02-03 | 2024-02-13 | 10X Genomics, Inc. | Increasing capture efficiency of spatial assays |
US11891654B2 (en) | 2020-02-24 | 2024-02-06 | 10X Genomics, Inc. | Methods of making gene expression libraries |
EP4139485B1 (de) | 2020-04-22 | 2023-09-06 | 10X Genomics, Inc. | Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau |
AU2021275906A1 (en) | 2020-05-22 | 2022-12-22 | 10X Genomics, Inc. | Spatial analysis to detect sequence variants |
WO2021236929A1 (en) | 2020-05-22 | 2021-11-25 | 10X Genomics, Inc. | Simultaneous spatio-temporal measurement of gene expression and cellular activity |
US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
US11981960B1 (en) | 2020-07-06 | 2024-05-14 | 10X Genomics, Inc. | Spatial analysis utilizing degradable hydrogels |
US11761038B1 (en) | 2020-07-06 | 2023-09-19 | 10X Genomics, Inc. | Methods for identifying a location of an RNA in a biological sample |
US11981958B1 (en) | 2020-08-20 | 2024-05-14 | 10X Genomics, Inc. | Methods for spatial analysis using DNA capture |
US11926822B1 (en) | 2020-09-23 | 2024-03-12 | 10X Genomics, Inc. | Three-dimensional spatial analysis |
US11827935B1 (en) | 2020-11-19 | 2023-11-28 | 10X Genomics, Inc. | Methods for spatial analysis using rolling circle amplification and detection probes |
US20240033741A1 (en) * | 2020-12-18 | 2024-02-01 | Hitachi High-Tech Corporation | Thermal cycler and genetic inspection apparatus |
EP4121555A1 (de) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Verfahren, zusammensetzungen und systeme zur erfassung von sonden und/oder barcodes |
WO2023034489A1 (en) | 2021-09-01 | 2023-03-09 | 10X Genomics, Inc. | Methods, compositions, and kits for blocking a capture probe on a spatial array |
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US4415732A (en) * | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
US5656493A (en) * | 1985-03-28 | 1997-08-12 | The Perkin-Elmer Corporation | System for automated performance of the polymerase chain reaction |
KR100236506B1 (ko) | 1990-11-29 | 2000-01-15 | 퍼킨-엘머시터스인스트루먼츠 | 폴리머라제 연쇄 반응 수행 장치 |
US5639423A (en) | 1992-08-31 | 1997-06-17 | The Regents Of The University Of Calfornia | Microfabricated reactor |
US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
US5965410A (en) * | 1997-09-02 | 1999-10-12 | Caliper Technologies Corp. | Electrical current for controlling fluid parameters in microchannels |
US6633726B2 (en) * | 1999-07-27 | 2003-10-14 | Kenneth A. Bradenbaugh | Method of controlling the temperature of water in a water heater |
US6337435B1 (en) | 1999-07-30 | 2002-01-08 | Bio-Rad Laboratories, Inc. | Temperature control for multi-vessel reaction apparatus |
WO2001055451A1 (en) * | 2000-01-28 | 2001-08-02 | Linden Technologies, Inc. | C-3' protected monomeric nucleotides and synthesis of oligonucleotides on solid support |
CA2405687A1 (en) * | 2000-04-05 | 2001-10-18 | Molecular Staging, Inc. | Process for allele discrimation utilizing primer extension |
EP1317569B1 (de) * | 2000-09-14 | 2009-11-18 | Caliper Life Sciences, Inc. | MIKROFLUIDISCHE VORRICHTUNGEN UND METHODEN UM TEMPERATUR-VERMITTELTE REAKTIONEN DURCHZUFüHREN |
AU2002247248B2 (en) * | 2001-03-02 | 2007-07-05 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | PCR method |
US20040038206A1 (en) * | 2001-03-14 | 2004-02-26 | Jia Zhang | Method for high throughput assay of genetic analysis |
US20040161741A1 (en) * | 2001-06-30 | 2004-08-19 | Elazar Rabani | Novel compositions and processes for analyte detection, quantification and amplification |
CN1245521C (zh) * | 2001-07-26 | 2006-03-15 | 黄道培 | Pcr扩增、杂交同步法 |
US6750661B2 (en) * | 2001-11-13 | 2004-06-15 | Caliper Life Sciences, Inc. | Method and apparatus for controllably effecting samples using two signals |
US20050064423A1 (en) * | 2002-01-08 | 2005-03-24 | Toshiro Higuchi | Pcr method by electrostatic transportation, hybridization method for electrostatic transportation and devices therefor |
AUPS267802A0 (en) * | 2002-05-30 | 2002-06-20 | Bio-Molecular Holdings Pty Limited | Improved dna amplification apparatus and method |
EP1522853A4 (de) | 2002-06-24 | 2005-10-19 | Canon Kk | Dna-mikro-array mit standard sonde und kit der diese enthaelt |
JP2004313181A (ja) * | 2003-04-02 | 2004-11-11 | Canon Inc | 感染症起炎菌検出用プローブ及びプローブセット、ならびに担体及び遺伝子検査方法 |
JP2005190427A (ja) * | 2003-12-26 | 2005-07-14 | Canon Inc | 配列を同定するための変異要素のセット抽出方法 |
-
2004
- 2004-04-26 JP JP2004130041A patent/JP4592060B2/ja not_active Expired - Fee Related
-
2005
- 2005-04-22 US US11/111,904 patent/US7569366B2/en not_active Expired - Fee Related
- 2005-04-25 CN CNB2005100669581A patent/CN100532567C/zh not_active Expired - Fee Related
- 2005-04-26 EP EP05252579A patent/EP1591543B1/de not_active Expired - Fee Related
- 2005-04-26 DE DE602005027144T patent/DE602005027144D1/de active Active
Also Published As
Publication number | Publication date |
---|---|
EP1591543B1 (de) | 2011-03-30 |
EP1591543A2 (de) | 2005-11-02 |
EP1591543A3 (de) | 2005-11-30 |
CN100532567C (zh) | 2009-08-26 |
JP4592060B2 (ja) | 2010-12-01 |
JP2005304445A (ja) | 2005-11-04 |
US20050239119A1 (en) | 2005-10-27 |
US7569366B2 (en) | 2009-08-04 |
CN1690218A (zh) | 2005-11-02 |
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