DE602005020691D1 - Rna-amplifikation auf ligationsbasis - Google Patents
Rna-amplifikation auf ligationsbasisInfo
- Publication number
- DE602005020691D1 DE602005020691D1 DE602005020691T DE602005020691T DE602005020691D1 DE 602005020691 D1 DE602005020691 D1 DE 602005020691D1 DE 602005020691 T DE602005020691 T DE 602005020691T DE 602005020691 T DE602005020691 T DE 602005020691T DE 602005020691 D1 DE602005020691 D1 DE 602005020691D1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- stranded region
- ligation
- basis
- rna amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US63893704P | 2004-12-23 | 2004-12-23 | |
| US68566105P | 2005-05-27 | 2005-05-27 | |
| PCT/US2005/046800 WO2006071776A2 (en) | 2004-12-23 | 2005-12-22 | Ligation-based rna amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602005020691D1 true DE602005020691D1 (de) | 2010-05-27 |
Family
ID=36499164
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602005020691T Active DE602005020691D1 (de) | 2004-12-23 | 2005-12-22 | Rna-amplifikation auf ligationsbasis |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20080003602A1 (de) |
| EP (1) | EP1836302B1 (de) |
| JP (1) | JP5420174B2 (de) |
| AT (1) | ATE464381T1 (de) |
| AU (1) | AU2005322131B2 (de) |
| CA (1) | CA2592425C (de) |
| DE (1) | DE602005020691D1 (de) |
| DK (1) | DK1836302T3 (de) |
| WO (1) | WO2006071776A2 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7727722B2 (en) * | 2001-08-29 | 2010-06-01 | General Electric Company | Ligation amplification |
| WO2009006438A2 (en) * | 2007-06-29 | 2009-01-08 | Epicentre Technologies Corporation | Copy dna and sense rna |
| GB0807321D0 (en) * | 2008-04-22 | 2008-05-28 | Oxford Gene Tech Ip Ltd | Analysis of nucleic acids |
| US20110053226A1 (en) * | 2008-06-13 | 2011-03-03 | Riboxx Gmbh | Method for enzymatic synthesis of chemically modified rna |
| US8753868B2 (en) | 2008-08-04 | 2014-06-17 | General Electric Company | Method and system for selective isolation of target biological molecules in a general purpose system |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| WO2013028902A2 (en) * | 2011-08-23 | 2013-02-28 | University Of Medicine And Dentistry Of New Jersey | Methods of isolating rna and mapping of polyadenylation isoforms |
| JP6375230B2 (ja) * | 2012-02-27 | 2018-08-15 | セルラー リサーチ， インコーポレイテッド | 分子計数のための組成物およびキット |
| WO2013130512A2 (en) | 2012-02-27 | 2013-09-06 | The University Of North Carolina At Chapel Hill | Methods and uses for molecular tags |
| GB201217770D0 (en) | 2012-10-04 | 2012-11-14 | Base4 Innovation Ltd | Biological probes and the use thereof |
| ES2900102T3 (es) * | 2013-02-20 | 2022-03-15 | Univ Emory | Composiciones para la secuenciación de ácidos nucleicos en mezclas |
| GB2525104B (en) | 2013-08-28 | 2016-09-28 | Cellular Res Inc | Massively Parallel Single Cell Nucleic Acid Analysis |
| US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| ES2824700T3 (es) | 2015-02-19 | 2021-05-13 | Becton Dickinson Co | Análisis unicelular de alto rendimiento que combina información proteómica y genómica |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| US11535882B2 (en) | 2015-03-30 | 2022-12-27 | Becton, Dickinson And Company | Methods and compositions for combinatorial barcoding |
| EP3286326A1 (de) | 2015-04-23 | 2018-02-28 | Cellular Research, Inc. | Verfahren und zusammensetzungen zur gesamttranskriptomamplifikation |
| CN107873055B (zh) | 2015-05-29 | 2021-09-17 | 库瑞瓦格房地产有限公司 | 包括至少一个切向流过滤步骤的产生和纯化rna的方法 |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| US11266337B2 (en) | 2015-09-09 | 2022-03-08 | Drawbridge Health, Inc. | Systems, methods, and devices for sample collection, stabilization and preservation |
| ES2745694T3 (es) | 2015-09-11 | 2020-03-03 | Cellular Res Inc | Métodos y composiciones para normalización de biblioteca de ácido nucleico |
| US11248223B2 (en) * | 2015-12-23 | 2022-02-15 | Curevac Ag | Method of RNA in vitro transcription using a buffer containing a dicarboxylic acid or tricarboxylic acid or a salt thereof |
| CN109072288A (zh) | 2016-05-02 | 2018-12-21 | 赛卢拉研究公司 | 精确的分子条形编码 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| EP3465502B1 (de) | 2016-05-26 | 2024-04-10 | Becton, Dickinson and Company | Verfahren zur anpassung des zählens von molekularen kennzeichen |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| WO2018089377A1 (en) | 2016-11-08 | 2018-05-17 | Cellular Research, Inc. | Methods for cell label classification |
| CN109952612B (zh) | 2016-11-08 | 2023-12-01 | 贝克顿迪金森公司 | 用于表达谱分类的方法 |
| ES2961580T3 (es) | 2017-01-13 | 2024-03-12 | Cellular Res Inc | Revestimiento hidrófilo de canales de fluidos |
| EP3577232A1 (de) | 2017-02-01 | 2019-12-11 | Cellular Research, Inc. | Selektive amplifikation unter verwendung von blockierenden oligonukleotiden |
| EP3635135A1 (de) | 2017-06-05 | 2020-04-15 | Becton, Dickinson and Company | Probenindizierung für einzelzellen |
| CA3078540A1 (en) | 2017-11-30 | 2019-06-06 | Arrakis Therapeutics, Inc. | Nucleic acid-binding photoprobes and uses thereof |
| EP3728636A1 (de) | 2017-12-19 | 2020-10-28 | Becton, Dickinson and Company | Mit oligonukleotiden assoziierte teilchen |
| EP3788170A1 (de) | 2018-05-03 | 2021-03-10 | Becton, Dickinson and Company | Molekulare barcodierung auf gegenüberliegenden transkriptenden |
| WO2019213294A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| WO2019236644A1 (en) * | 2018-06-05 | 2019-12-12 | Arrakis Therapeutics, Inc. | Encoded libraries and methods of use for screening nucleic acid targets |
| EP3861134A1 (de) | 2018-10-01 | 2021-08-11 | Becton, Dickinson and Company | Bestimmung von 5'-transkriptsequenzen |
| WO2020097315A1 (en) | 2018-11-08 | 2020-05-14 | Cellular Research, Inc. | Whole transcriptome analysis of single cells using random priming |
| EP3894552A1 (de) | 2018-12-13 | 2021-10-20 | Becton, Dickinson and Company | Selektive verlängerung in der vollständigen transkriptomanalyse von einzelzellen |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| EP4242322A3 (de) | 2019-01-23 | 2023-09-20 | Becton, Dickinson and Company | Mit antikörpern assoziierte oligonukleotide |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| CN114051534A (zh) | 2019-07-22 | 2022-02-15 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| US11773436B2 (en) | 2019-11-08 | 2023-10-03 | Becton, Dickinson And Company | Using random priming to obtain full-length V(D)J information for immune repertoire sequencing |
| EP4090763A1 (de) | 2020-01-13 | 2022-11-23 | Becton Dickinson and Company | Verfahren und zusammensetzungen zur quantifizierung von proteinen und rna |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA2001110A1 (en) * | 1988-11-18 | 1990-05-18 | Zvi G. Loewy | Compositions and process for amplifying and detecting nucleic acid sequences |
| US5545522A (en) | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| EP1396540B1 (de) * | 1990-09-27 | 2007-02-07 | Invitrogen Corporation | Direkte Klonierung von PCR amplifizierten Nucleinsäuren |
| US5658736A (en) * | 1996-01-16 | 1997-08-19 | Genetics Institute, Inc. | Oligonucleotide population preparation |
| WO1998020166A2 (en) * | 1996-11-06 | 1998-05-14 | Sequenom, Inc. | Dna diagnostics based on mass spectrometry |
| EP1712623B1 (de) * | 1997-01-21 | 2011-10-19 | The General Hospital Corporation | Selektion von Proteinen unter Verwendung von RNA-Protein-Fusionen |
| FR2761695B1 (fr) * | 1997-04-04 | 1999-09-24 | Bio Merieux | Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation |
| KR100440044B1 (ko) * | 1998-02-27 | 2004-07-14 | 팸진 비.브이. | 핵산의 비특이적 증폭방법 |
| JP3883476B2 (ja) * | 1999-03-19 | 2007-02-21 | タカラバイオ株式会社 | 核酸配列の増幅方法 |
| US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
| ATE455186T1 (de) * | 2000-06-26 | 2010-01-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
| FI115139B (fi) * | 2001-01-10 | 2005-03-15 | Valtion Teknillinen | Menetelmä ja testipakkaus solu- tai kudosnäytteissä olevien polynukleotidien määrässä tapahtuvien vaihteluiden kvantitatiiviseen ja/tai vertailevaan arvioimiseen |
| US20030104432A1 (en) * | 2001-07-27 | 2003-06-05 | The Regents Of The University Of California | Methods of amplifying sense strand RNA |
| US7727722B2 (en) * | 2001-08-29 | 2010-06-01 | General Electric Company | Ligation amplification |
| CA2493808A1 (en) * | 2002-07-24 | 2004-01-29 | Ptc Therapeutics, Inc. | Methods for identifying small molecules that modulate premature translation termination and nonsense mediated mrna decay |
| CA2501983A1 (en) | 2002-10-30 | 2004-05-27 | Pamgene B.V. | Improved methods for generating multiple rna copies |
| GB0228289D0 (en) * | 2002-12-04 | 2003-01-08 | Genome Inst Of Singapore Nat U | Method |
| EP2374900B1 (de) * | 2003-03-07 | 2016-07-13 | Rubicon Genomics, Inc. | Polynukleotide zur amplifikation und analyse von gesamtgenom- und gesamttranskriptom-bibliotheken, die durch ein dns-polymerisierungsverfahren erzeugt wurden |
| US20060019258A1 (en) * | 2004-07-20 | 2006-01-26 | Illumina, Inc. | Methods and compositions for detection of small interfering RNA and micro-RNA |
-
2005
- 2005-12-22 WO PCT/US2005/046800 patent/WO2006071776A2/en active Application Filing
- 2005-12-22 CA CA2592425A patent/CA2592425C/en active Active
- 2005-12-22 JP JP2007548525A patent/JP5420174B2/ja active Active
- 2005-12-22 DK DK05855371.0T patent/DK1836302T3/da active
- 2005-12-22 DE DE602005020691T patent/DE602005020691D1/de active Active
- 2005-12-22 AU AU2005322131A patent/AU2005322131B2/en active Active
- 2005-12-22 EP EP05855371A patent/EP1836302B1/de active Active
- 2005-12-22 US US11/722,089 patent/US20080003602A1/en not_active Abandoned
- 2005-12-22 AT AT05855371T patent/ATE464381T1/de active
-
2018
- 2018-04-30 US US15/966,353 patent/US10704042B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20180320172A1 (en) | 2018-11-08 |
| AU2005322131A1 (en) | 2006-07-06 |
| EP1836302B1 (de) | 2010-04-14 |
| CA2592425A1 (en) | 2006-07-06 |
| US20080003602A1 (en) | 2008-01-03 |
| WO2006071776A2 (en) | 2006-07-06 |
| AU2005322131B2 (en) | 2011-11-10 |
| EP1836302A2 (de) | 2007-09-26 |
| US10704042B2 (en) | 2020-07-07 |
| JP2008525038A (ja) | 2008-07-17 |
| DK1836302T3 (da) | 2010-08-02 |
| WO2006071776A3 (en) | 2006-09-14 |
| ATE464381T1 (de) | 2010-04-15 |
| JP5420174B2 (ja) | 2014-02-19 |
| CA2592425C (en) | 2014-06-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition |