DE602004023140D1 - Rhamnose-induzierbares Expressionssystem - Google Patents
Rhamnose-induzierbares ExpressionssystemInfo
- Publication number
- DE602004023140D1 DE602004023140D1 DE200460023140 DE602004023140T DE602004023140D1 DE 602004023140 D1 DE602004023140 D1 DE 602004023140D1 DE 200460023140 DE200460023140 DE 200460023140 DE 602004023140 T DE602004023140 T DE 602004023140T DE 602004023140 D1 DE602004023140 D1 DE 602004023140D1
- Authority
- DE
- Germany
- Prior art keywords
- rhamnose
- expression system
- inducible expression
- inducible
- present
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/73—Expression systems using phage (lambda) regulatory sequences
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
- G16B50/30—Data warehousing; Computing architectures
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/678,961 US8293503B2 (en) | 2003-10-03 | 2003-10-03 | Vectors for directional cloning |
US10/702,228 US20050074785A1 (en) | 2003-10-03 | 2003-11-05 | Vectors for directional cloning |
Publications (1)
Publication Number | Publication Date |
---|---|
DE602004023140D1 true DE602004023140D1 (de) | 2009-10-22 |
Family
ID=34394063
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE200460023140 Active DE602004023140D1 (de) | 2003-10-03 | 2004-09-29 | Rhamnose-induzierbares Expressionssystem |
DE200460024107 Active DE602004024107D1 (de) | 2003-10-03 | 2004-09-29 | Vektoren zur gerichteten klonierung |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE200460024107 Active DE602004024107D1 (de) | 2003-10-03 | 2004-09-29 | Vektoren zur gerichteten klonierung |
Country Status (6)
Country | Link |
---|---|
US (7) | US8293503B2 (de) |
EP (1) | EP2116601B1 (de) |
JP (3) | JP5456706B2 (de) |
AT (2) | ATE448303T1 (de) |
DE (2) | DE602004023140D1 (de) |
DK (2) | DK1847611T3 (de) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080050808A1 (en) * | 2002-10-09 | 2008-02-28 | Reed Thomas D | DNA modular cloning vector plasmids and methods for their use |
US7785871B2 (en) * | 2002-10-09 | 2010-08-31 | Intrexon Corporation | DNA cloning vector plasmids and methods for their use |
CA2541177A1 (en) * | 2003-10-03 | 2005-09-22 | Promega Corporation | Vectors for directional cloning |
US8293503B2 (en) * | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
WO2005089110A2 (en) * | 2004-02-27 | 2005-09-29 | President And Fellows Of Harvard College | Polynucleotide synthesis |
US20050227316A1 (en) * | 2004-04-07 | 2005-10-13 | Kosan Biosciences, Inc. | Synthetic genes |
WO2005116231A1 (en) | 2004-05-18 | 2005-12-08 | Intrexon Corporation | Methods for dynamic vector assembly of dna cloning vector plasmids |
WO2007005053A1 (en) * | 2005-06-30 | 2007-01-11 | Codon Devices, Inc. | Hierarchical assembly methods for genome engineering |
US8153598B2 (en) * | 2005-10-19 | 2012-04-10 | Intrexon Corporation | PKD ligands and polynucleotides encoding PKD ligands |
EP1842915A1 (de) * | 2006-04-04 | 2007-10-10 | Libragen | In vitro Methode zur Polynukleotide Sequenz Durchmischung durch die Fragmentierung und Ligierung von rekursiv zirkulären DNA Molekülen |
WO2008017073A2 (en) * | 2006-08-03 | 2008-02-07 | Wisconsin Alumni Research Foundation | Vector systems |
WO2008051619A2 (en) * | 2006-10-24 | 2008-05-02 | Los Alamos National Security, Llc | Vectors and methods for selecting open reading frames |
EP2087107A2 (de) | 2006-10-30 | 2009-08-12 | Promega Corporation | Mutante hydrolaseproteine mit verstärkten kinetischen eigenschaften und funktioneller expression |
US8323930B2 (en) * | 2007-07-28 | 2012-12-04 | Dna Twopointo, Inc. | Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase |
WO2012167083A2 (en) * | 2011-06-02 | 2012-12-06 | Albert Einstein College Of Medicine Of Yeshiva University | Method for measuring somatic dna mutational profiles |
EP2802655A4 (de) * | 2012-01-13 | 2015-11-18 | Momenta Pharmaceuticals Inc | Nucleotidklonungsverfahren |
US10829772B2 (en) | 2012-06-22 | 2020-11-10 | Monsanto Technology Llc | Unique modular vector design |
US11072811B2 (en) | 2013-03-15 | 2021-07-27 | Promega Corporation | Substrates for covalent tethering of proteins to functional groups or solid surfaces |
EP3130673B1 (de) * | 2015-08-13 | 2019-06-12 | Centrillion Technology Holdings Corporation | Bibliothekkonstruktion mit y-adaptern und schwindenden restriktionsstellen |
RU2610179C1 (ru) * | 2015-11-06 | 2017-02-08 | Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) | Способ получения рекомбинантного противоопухолевого токсина на основе белков барназа-барстар и адресного полипептида дарпина с эффектом моментальной отмены цитотоксического действия |
US11618899B2 (en) | 2016-02-09 | 2023-04-04 | Brookhaven Science Associates, Llc | Cloning and expression vectors and systems |
CN106222186B (zh) * | 2016-08-22 | 2019-03-19 | 河北省农林科学院粮油作物研究所 | 入门载体pAmpGate和pGentGate的构建及使用方法 |
US10385319B2 (en) | 2016-09-08 | 2019-08-20 | The Governement of the United States of America, as represented by the Secretary of Homeland Security | Modified foot-and-mouth disease virus 3C proteases, compositions and methods thereof |
US10435695B2 (en) | 2016-09-08 | 2019-10-08 | The Government of the United States of America, as represented by the Secretary of Homeland Security | Fusion protein comprising Gaussia luciferase, translation interrupter sequence, and interferon amino acid sequences |
US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
AU2020205621A1 (en) * | 2019-01-09 | 2021-08-19 | Asimov, Inc. | Compositions of polynucleic acid vectors and uses thereof |
CN111235638A (zh) * | 2020-02-20 | 2020-06-05 | 北京良芯生物科技发展有限公司 | 一种构建测序文库的方法及试剂盒 |
WO2021249536A1 (zh) * | 2020-06-12 | 2021-12-16 | 南京金斯瑞生物科技有限公司 | 含barstar基因的工程菌及其在barnase基因克隆中的应用 |
EP3981879A1 (de) * | 2020-10-09 | 2022-04-13 | Stada Arzneimittel Ag | Verfahren zur herstellung eines rekombinanten proteins in einer wirtszelle mit einem deaktivierten rhamnosemetabolismus sowie expressionsvektoren, wirtszellen und rekombinanten proteinen davon |
Family Cites Families (61)
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US815951A (en) | 1903-10-15 | 1906-03-27 | American Textile Specialty Machinery Company | Weft-replenishing loom. |
US4403036A (en) * | 1980-12-02 | 1983-09-06 | University Of Iowa Research Foundation | Genetic reagents for generating plasmids containing multiple copies of DNA segments |
US4724202A (en) * | 1983-12-12 | 1988-02-09 | Molecular Diagnostics, Inc. | Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization |
US4784949A (en) * | 1984-04-19 | 1988-11-15 | Cetus Corporation | Universal dominant selectable marker cassette |
US4952496A (en) * | 1984-03-30 | 1990-08-28 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
US5693489A (en) * | 1984-03-30 | 1997-12-02 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
EP0178863A1 (de) | 1984-10-15 | 1986-04-23 | Schering Corporation | Expressionssysteme die sich der Bakteriophage-T7-Promotoren bedienen und Gensequenzen |
US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
US4808537A (en) | 1984-10-30 | 1989-02-28 | Phillips Petroleum Company | Methanol inducible genes obtained from pichia and methods of use |
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CA2506999C (en) | 2002-12-02 | 2014-01-07 | Basf Aktiengesellschaft | L-rhamnose-inducible expression systems |
US8293503B2 (en) * | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
CA2541177A1 (en) * | 2003-10-03 | 2005-09-22 | Promega Corporation | Vectors for directional cloning |
-
2003
- 2003-10-03 US US10/678,961 patent/US8293503B2/en active Active
- 2003-11-05 US US10/702,228 patent/US20050074785A1/en not_active Abandoned
-
2004
- 2004-09-29 DE DE200460023140 patent/DE602004023140D1/de active Active
- 2004-09-29 AT AT04821603T patent/ATE448303T1/de not_active IP Right Cessation
- 2004-09-29 AT AT09011477T patent/ATE539156T1/de active
- 2004-09-29 DK DK07013756T patent/DK1847611T3/da active
- 2004-09-29 EP EP20090011477 patent/EP2116601B1/de active Active
- 2004-09-29 DK DK04821603T patent/DK1685247T3/da active
- 2004-09-29 DE DE200460024107 patent/DE602004024107D1/de active Active
- 2004-11-12 US US10/987,411 patent/US20050130205A1/en not_active Abandoned
-
2009
- 2009-12-28 US US12/648,119 patent/US8367403B2/en not_active Expired - Lifetime
-
2011
- 2011-02-03 JP JP2011021710A patent/JP5456706B2/ja active Active
- 2011-10-17 US US13/274,637 patent/US9469857B2/en active Active
- 2011-10-17 US US13/274,643 patent/US9018014B2/en active Active
-
2013
- 2013-01-14 US US13/740,337 patent/US9371531B2/en not_active Expired - Lifetime
- 2013-08-14 JP JP2013168742A patent/JP5899168B2/ja active Active
-
2016
- 2016-01-21 JP JP2016009792A patent/JP6341941B2/ja active Active
Also Published As
Publication number | Publication date |
---|---|
DE602004024107D1 (de) | 2009-12-24 |
DK1847611T3 (da) | 2010-01-04 |
ATE539156T1 (de) | 2012-01-15 |
EP2116601A2 (de) | 2009-11-11 |
JP2011083296A (ja) | 2011-04-28 |
US8367403B2 (en) | 2013-02-05 |
JP5456706B2 (ja) | 2014-04-02 |
US20050130205A1 (en) | 2005-06-16 |
US20050074785A1 (en) | 2005-04-07 |
US9469857B2 (en) | 2016-10-18 |
JP2016052338A (ja) | 2016-04-14 |
US20130183760A1 (en) | 2013-07-18 |
US20050074883A1 (en) | 2005-04-07 |
JP6341941B2 (ja) | 2018-06-13 |
US9371531B2 (en) | 2016-06-21 |
US20100216231A1 (en) | 2010-08-26 |
JP5899168B2 (ja) | 2016-04-06 |
US8293503B2 (en) | 2012-10-23 |
JP2013247960A (ja) | 2013-12-12 |
EP2116601B1 (de) | 2011-12-28 |
DK1685247T3 (da) | 2010-03-08 |
ATE448303T1 (de) | 2009-11-15 |
EP2116601A3 (de) | 2010-06-09 |
US20140186959A1 (en) | 2014-07-03 |
US9018014B2 (en) | 2015-04-28 |
US20130164837A1 (en) | 2013-06-27 |
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