DE60045586D1 - TEST METHOD FOR MEASURING PHOSPHORYLATION - Google Patents

TEST METHOD FOR MEASURING PHOSPHORYLATION

Info

Publication number
DE60045586D1
DE60045586D1 DE60045586T DE60045586T DE60045586D1 DE 60045586 D1 DE60045586 D1 DE 60045586D1 DE 60045586 T DE60045586 T DE 60045586T DE 60045586 T DE60045586 T DE 60045586T DE 60045586 D1 DE60045586 D1 DE 60045586D1
Authority
DE
Germany
Prior art keywords
test method
measuring phosphorylation
modifications
phosphorylation
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60045586T
Other languages
German (de)
Inventor
J Richard Sportsman
Merl F Hoekstra
Sandra K Lee
Nicholas Cairns
Lawrence M Kauvar
Huang Wei
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Devices LLC
Original Assignee
Molecular Devices LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Devices LLC filed Critical Molecular Devices LLC
Application granted granted Critical
Publication of DE60045586D1 publication Critical patent/DE60045586D1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Steroid Compounds (AREA)

Abstract

Assays for detecting phosphorylation and dephosphorylation modifications of proteins, along with the presence, activity, and modulation of enzymes effecting these modifications, including kinases and phosphatases, respectively.
DE60045586T 1999-06-09 2000-06-09 TEST METHOD FOR MEASURING PHOSPHORYLATION Expired - Lifetime DE60045586D1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US13831199P 1999-06-09 1999-06-09
US13843899P 1999-06-10 1999-06-10
US34973399A 1999-07-08 1999-07-08
US20059400P 2000-04-28 2000-04-28
PCT/US2000/016025 WO2000075167A2 (en) 1999-06-09 2000-06-09 Phosphorylation assays

Publications (1)

Publication Number Publication Date
DE60045586D1 true DE60045586D1 (en) 2011-03-10

Family

ID=27495318

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60045586T Expired - Lifetime DE60045586D1 (en) 1999-06-09 2000-06-09 TEST METHOD FOR MEASURING PHOSPHORYLATION

Country Status (5)

Country Link
EP (1) EP1261621B1 (en)
AT (1) ATE497164T1 (en)
AU (1) AU5604900A (en)
DE (1) DE60045586D1 (en)
WO (1) WO2000075167A2 (en)

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US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
GB9923208D0 (en) * 1999-10-01 1999-12-08 Cambridge Drug Discovery Ltd Assay
GB0007915D0 (en) * 2000-03-31 2000-05-17 Univ Bristol Protein kinase assay
US20030162230A1 (en) * 2000-09-27 2003-08-28 Reagan Kevin J. Method for quantifying phosphokinase activity on proteins
US20090118139A1 (en) * 2000-11-07 2009-05-07 Caliper Life Sciences, Inc. Microfluidic method and system for enzyme inhibition activity screening
US6811990B1 (en) 2001-02-13 2004-11-02 Michael J. Corey Methods and compositions for coupled luminescent assays
EP1264897A3 (en) * 2001-06-06 2003-11-12 Europäisches Laboratorium Für Molekularbiologie (Embl) Synthetic sensor peptide for kinase or phosphatase assays
WO2003014698A2 (en) * 2001-08-07 2003-02-20 Merck & Co., Inc. Biological assay detection method
US8623822B2 (en) 2002-03-01 2014-01-07 Bracco Suisse Sa KDR and VEGF/KDR binding peptides and their use in diagnosis and therapy
US7794693B2 (en) 2002-03-01 2010-09-14 Bracco International B.V. Targeting vector-phospholipid conjugates
WO2004065621A1 (en) 2002-03-01 2004-08-05 Dyax Corp. Kdr and vegf/kdr binding peptides and their use in diagnosis and therapy
US7261876B2 (en) 2002-03-01 2007-08-28 Bracco International Bv Multivalent constructs for therapeutic and diagnostic applications
DE10323081A1 (en) * 2003-05-22 2004-12-16 Aventis Pharma Deutschland Gmbh Use of a polypeptide
JP2007505315A (en) * 2003-09-12 2007-03-08 インヴィトロジェン コーポレーション Multiple binding and activity assays
US7282339B2 (en) * 2003-09-17 2007-10-16 Invitrogen Corporation Competitive immunoassay
MX2007002998A (en) * 2004-09-17 2007-05-18 Chiron Corp Trivalent metal mediated homogeneous luminescent proximity assay.
WO2007016975A1 (en) 2005-07-29 2007-02-15 F. Hoffmann-La Roche Ag Kinase and phosphatase assays based on fret
FI20070171A0 (en) * 2007-02-28 2007-02-28 Hidex Oy Bioassay and bioassay equipment
GB2452076A (en) 2007-08-23 2009-02-25 Mologic Ltd Detection of enzymes by detecting binding of substrate recognition molecules to modified substrates
WO2010117420A2 (en) * 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
ES2555268T3 (en) * 2010-12-21 2015-12-30 Gene Signal International Sa Peptides to treat cancer
US8383591B2 (en) 2010-12-21 2013-02-26 Gene Signal International S.A. Peptides for treating cancer

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5235039A (en) * 1991-06-10 1993-08-10 Eli Lilly And Company Substrates for hiv protease
US5952236A (en) * 1995-10-26 1999-09-14 Thompson; Richard B. Enzyme-based fluorescence biosensor for chemical analysis
US6097025A (en) 1997-10-31 2000-08-01 Ljl Biosystems, Inc. Light detection device having an optical-path switching mechanism
US6071748A (en) 1997-07-16 2000-06-06 Ljl Biosystems, Inc. Light detection device
WO1999042817A1 (en) 1998-02-20 1999-08-26 Ljl Biosystems, Inc. Broad range light detection system
WO2000004364A2 (en) 1998-07-15 2000-01-27 Ljl Biosystems, Inc. Evanescent field illumination devices and methods
WO2000006989A2 (en) 1998-07-27 2000-02-10 Ljl Biosystems, Inc. Apparatus and methods for identifying quenching effects in luminescence assays
WO2000006991A2 (en) 1998-07-27 2000-02-10 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
WO2000005336A2 (en) 1998-07-22 2000-02-03 Ljl Biosystems, Inc. Devices and methods for sample analysis
JP2003521670A (en) 1997-10-31 2003-07-15 エルジェイエル・バイオシステムズ・インコーポレーテッド Apparatus and method for measuring fluorescence polarization
AU2344899A (en) 1998-01-26 1999-08-09 Ljl Biosystems, Inc. Apparatus and methods for improving signal resolution in optical spectroscopy
EP1071942A1 (en) 1998-04-17 2001-01-31 LJL Biosystems, Inc. Sample-holding devices and systems
AU5667599A (en) 1998-07-27 2000-02-21 Ljl Biosystems, Inc. Apparatus and methods for time-resolved spectroscopic measurements
AU1219800A (en) * 1998-10-20 2000-05-08 Ljl Biosystems, Inc. Improvements in luminescence assays
WO2000047693A1 (en) * 1999-02-12 2000-08-17 Ljl Biosystems, Inc. Luminescent metal-ligand complexes
US6573059B1 (en) * 1999-06-04 2003-06-03 Rmf Dictagene S.A. Use of the regulatory subunit of the camp dependent protein kinase (PKA) from dictyostelium for camp measurements

Also Published As

Publication number Publication date
EP1261621B1 (en) 2011-01-26
WO2000075167A2 (en) 2000-12-14
WO2000075167A9 (en) 2001-06-28
AU5604900A (en) 2000-12-28
WO2000075167A3 (en) 2002-09-12
ATE497164T1 (en) 2011-02-15
EP1261621A4 (en) 2007-12-12
EP1261621A2 (en) 2002-12-04

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