DE60043992D1 - Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs) - Google Patents

Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs)

Info

Publication number
DE60043992D1
DE60043992D1 DE60043992T DE60043992T DE60043992D1 DE 60043992 D1 DE60043992 D1 DE 60043992D1 DE 60043992 T DE60043992 T DE 60043992T DE 60043992 T DE60043992 T DE 60043992T DE 60043992 D1 DE60043992 D1 DE 60043992D1
Authority
DE
Germany
Prior art keywords
protein
plant
expression
ptgs
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60043992T
Other languages
English (en)
Inventor
David Charles Baulcombe
Olivier Voinnet
Andrew John Hamilton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plant Bioscience Ltd
Original Assignee
Plant Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9927609.9A external-priority patent/GB9927609D0/en
Priority claimed from GB0022960A external-priority patent/GB0022960D0/en
Priority claimed from GB0023668A external-priority patent/GB0023668D0/en
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Application granted granted Critical
Publication of DE60043992D1 publication Critical patent/DE60043992D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
DE60043992T 1999-11-22 2000-11-22 Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs) Expired - Lifetime DE60043992D1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9927609.9A GB9927609D0 (en) 1999-11-22 1999-11-22 Enhanced expression
GB0022960A GB0022960D0 (en) 2000-09-19 2000-09-19 Enhanced expression
GB0023668A GB0023668D0 (en) 2000-09-27 2000-09-27 Enhanced expression
PCT/GB2000/004454 WO2001038512A2 (en) 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs)

Publications (1)

Publication Number Publication Date
DE60043992D1 true DE60043992D1 (de) 2010-04-22

Family

ID=27255894

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60043992T Expired - Lifetime DE60043992D1 (de) 1999-11-22 2000-11-22 Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs)

Country Status (12)

Country Link
US (2) US7217854B1 (de)
EP (1) EP1232274B1 (de)
JP (1) JP4884628B2 (de)
KR (1) KR20030004305A (de)
AT (1) ATE460490T1 (de)
AU (1) AU782788B2 (de)
CA (1) CA2390152C (de)
DE (1) DE60043992D1 (de)
IL (1) IL149388A0 (de)
MX (1) MXPA02005089A (de)
NZ (1) NZ518947A (de)
WO (1) WO2001038512A2 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
AU784336B2 (en) * 1999-11-12 2006-03-16 University Of South Carolina Control of post-transcriptional gene silencing in plants
GB0101505D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst Protection against gene silencing
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
EP1551967B1 (de) * 2002-07-19 2011-08-31 University Of South Carolina Zusammensetzungen und verfahren zur modulation der genexpression in pflanzen
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
WO2005033306A1 (ja) * 2003-10-01 2005-04-14 Japan Science And Technology Agency 形質転換細胞および該細胞を用いたタンパク質の生産方法並びにタンパク質生産キット、ウイルスベクター発現用ｄｎａ断片およびその利用、タンパク質生産用形質転換体の生産方法および当該生産方法によって得られたタンパク質生産用形質転換体並びにその利用
GB0327919D0 (en) * 2003-12-02 2004-01-07 Plant Bioscience Ltd Enhanced expression
CA2555230A1 (en) * 2004-02-20 2005-09-09 Fraunhofer Usa Inc. Systems and methods for clonal expression in plants
CA3023314C (en) 2004-04-22 2019-12-10 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US7807849B2 (en) 2004-04-22 2010-10-05 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
EP1616959A1 (de) * 2004-07-07 2006-01-18 Icon Genetics AG Biologisch sichere transiente Proteinexpression in Pflanzen
FR2877014B1 (fr) * 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
FR2880357B1 (fr) 2005-01-05 2010-12-10 Inst Rech Developpement Ird Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications
CA2640473A1 (en) * 2006-02-03 2007-08-16 The Texas A & M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based p19 gene mutants
EP2059588A4 (de) 2006-08-29 2010-07-28 Commw Scient Ind Res Org Fettsäuresynthese
EP1897953A1 (de) 2006-09-06 2008-03-12 Icon Genetics GmbH Replicon von Potexviren stammend
WO2008036424A2 (en) * 2006-09-22 2008-03-27 Regents Of The University Of California Chemically inducible cucumber mosaic virus protein expression system
US8936937B2 (en) 2007-01-29 2015-01-20 The Ohio State University Research Foundation System for expression of genes in plants from a virus-based expression vector
EP2586866A1 (de) * 2007-05-09 2013-05-01 Devgen NV Verfahren für nachhaltige Transgentranskription
AU2009285667B2 (en) * 2008-08-27 2015-05-28 Arizona Board Of Regents For And On Behalf Of Arizona State University A DNA replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
AU2013204270B8 (en) * 2008-11-18 2015-06-04 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
DK2358882T3 (da) 2008-11-18 2017-11-06 Commw Scient Ind Res Org Enzymer og fremgangsmåder til fremstilling af omega-3 fedtsyrer
US8344208B2 (en) * 2009-02-26 2013-01-01 Baylor University Highly efficient suppressor-dependent protein expression in plants with a viral vector
US20110197312A1 (en) * 2010-02-07 2011-08-11 University Of Kuwait Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi
FR2960550A1 (fr) * 2010-05-26 2011-12-02 Inst Rech R Pour Le Dev Ird Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique
FI20100248A0 (fi) 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
SG192022A1 (en) 2011-01-17 2013-08-30 Philip Morris Prod Protein expression in plants
CN103502455A (zh) 2011-01-17 2014-01-08 菲利普莫里斯生产公司 用于在植物中核酸表达的载体
KR101334846B1 (ko) * 2011-07-20 2013-11-29 대한민국 벼줄무늬잎마름병 바이러스 유래 ｎｓ3유전자의 신규 용도
EP2798065B1 (de) * 2011-12-27 2019-06-05 Commonwealth Scientific and Industrial Research Organisation Gleichzeitiges gen-silencing sowie unterdrückung von gen-silencing in derselben zelle
KR102197208B1 (ko) 2012-06-15 2021-01-04 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 식물 세포에서 장쇄 다중불포화 지방산의 생성
PE20170253A1 (es) 2013-12-18 2017-04-14 Nuseed Pty Ltd Lipido que comprende acidos grasos poliinsaturados de cadena larga
WO2015168733A1 (en) * 2014-05-05 2015-11-12 Queensland University Of Technology Decoy molecules
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
US11649465B2 (en) 2017-09-11 2023-05-16 R.J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
KR20210101227A (ko) * 2018-11-13 2021-08-18 유니버시티 오브 매릴랜드, 칼리지 파크 식물 벡터, 조성물 및 그것들과 관련된 용도
CN115851820B (zh) * 2022-12-19 2024-06-18 内蒙古农业大学 一株烟草脉斑驳病毒的应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939541A (en) * 1997-03-28 1999-08-17 University Of South Carolina Method for enhancing expression of a foreign or endogenous gene product in plants
AU784336B2 (en) * 1999-11-12 2006-03-16 University Of South Carolina Control of post-transcriptional gene silencing in plants
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants

Also Published As

Publication number Publication date
KR20030004305A (ko) 2003-01-14
JP4884628B2 (ja) 2012-02-29
MXPA02005089A (es) 2003-01-28
EP1232274A2 (de) 2002-08-21
AU1533401A (en) 2001-06-04
NZ518947A (en) 2004-05-28
US7217854B1 (en) 2007-05-15
WO2001038512A3 (en) 2002-03-28
CA2390152A1 (en) 2001-05-31
US20070292862A1 (en) 2007-12-20
AU782788B2 (en) 2005-08-25
IL149388A0 (en) 2002-11-10
US8871997B2 (en) 2014-10-28
WO2001038512A2 (en) 2001-05-31
EP1232274B1 (de) 2010-03-10
JP2003514585A (ja) 2003-04-22
CA2390152C (en) 2012-08-28
ATE460490T1 (de) 2010-03-15

Similar Documents

Publication Publication Date Title
DE60043992D1 (de) Erhöhte expression von transgenen durch co-exprimierung mit einen suppressor der post-transkriptionelle genabschaltung (ptgs)
Dolja et al. Deep roots and splendid boughs of the global plant virome
Voinnet et al. Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants
NO20015587D0 (no) Ekspresjon og eksport av interferon-<alfa> proteiner som Fc- fusjonsproteiner
Masuta et al. Development of clover yellow vein virus as an efficient, stable gene‐expression system for legume species
JP2003514585A5 (de)
Swaney et al. RNA-mediated resistance with nonstructural genes from the tobacco etch virus genome.
DK1144662T3 (da) Fremgangsmåde og midler til ændring af planteegenskaber under anvendelse af vernaliseringsgenet VRN2
DK1104457T3 (da) Fremgangsmåde til in vitro-fremstilling af rekombinante polynukleotidsekvenser, således opnåede sekvensbanker og sekvenser
JP2012501182A5 (de)
CY1114598T1 (el) Μεθοδος για την ενισχυση ενος ιου φυσαλιδωδους νοσου υπο συνθηκες ελευθερες ορου
ES2194865T3 (es) Amplificacion viral de arn mensajero recombinante en plantas transgenicas.
AR014155A1 (es) Proteina aislada involucrada en la resistencia a nematodos, secuencia de adn aislada, vector de transformacion, planta, semillas y metodo para aumentarla resistencia a nematodos en una planta
WO2003087313A3 (en) Enhanced silk exsertion under stress
WO2004044161A3 (en) Expression of foreign sequences in plants using trans-activation system
DK0724639T3 (da) Fremgangsmåder til udvælgelse af rekombinante værtsceller, der udtrykker høje niveauer af et ønsket protein
ATE391768T1 (de) Verpackungszelle
RU96100552A (ru) Конструкция днк (варианты), растения, содержащие такую конструкцию, и способ их получения
AR011740A1 (es) Proteinas del virus que produce enrollamiento en las hojas de la vid del tipo 2, secuencias de codificacion y metodos
Majer et al. Relocation of the NIb gene in the tobacco etch potyvirus genome
DE60042922D1 (de) Virale expressionsvektoren für pflanzen
US20050260166A1 (en) Expressional enhancers from viruses
Liu et al. Genome sequence of a small RNA virus of the southern corn rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae)
Van Den Boogaart et al. Sources of target specificity associated with the recovery against Pea seed‐borne mosaic virus infection mediated by RNA silencing in pea
Verhoeven et al. Iresine viroid 1 and a potential new pospiviroid from portulaca

Legal Events

Date Code Title Description
8364 No opposition during term of opposition