DE60037886D1 - Verfahren zur reinigung von rekombinanten proteinen, welche wachstumshormon antagonisten sind, mittels wässriger zweiphasen-extraktion - Google Patents

Verfahren zur reinigung von rekombinanten proteinen, welche wachstumshormon antagonisten sind, mittels wässriger zweiphasen-extraktion

Info

Publication number
DE60037886D1
DE60037886D1 DE60037886T DE60037886T DE60037886D1 DE 60037886 D1 DE60037886 D1 DE 60037886D1 DE 60037886 T DE60037886 T DE 60037886T DE 60037886 T DE60037886 T DE 60037886T DE 60037886 D1 DE60037886 D1 DE 60037886D1
Authority
DE
Germany
Prior art keywords
growth hormone
phase extraction
aquatic
cleaning
recombinant proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60037886T
Other languages
English (en)
Other versions
DE60037886T2 (de
Inventor
Kirk J Hayenga
Pascal P Valex
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Covance Biotechnology Services Inc
Original Assignee
Covance Biotechnology Services Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Covance Biotechnology Services Inc filed Critical Covance Biotechnology Services Inc
Application granted granted Critical
Publication of DE60037886D1 publication Critical patent/DE60037886D1/de
Publication of DE60037886T2 publication Critical patent/DE60037886T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Endocrinology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60037886T 1999-05-07 2000-05-04 Verfahren für die Reinigung von rekombinanten Wachstumshormonantagonist-Proteinen unter Verwendung von Zwei-Phasen-flüssig-flüssig-Extraktionsverfahrensweisen Expired - Fee Related DE60037886T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US307549 1999-05-07
US09/307,549 US6437101B1 (en) 1999-05-07 1999-05-07 Methods for protein purification using aqueous two-phase extraction
PCT/US2000/012320 WO2000068260A1 (en) 1999-05-07 2000-05-04 Methods for protein purification using aqueous two-phase extraction

Publications (2)

Publication Number Publication Date
DE60037886D1 true DE60037886D1 (de) 2008-03-13
DE60037886T2 DE60037886T2 (de) 2009-01-22

Family

ID=23190235

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60037886T Expired - Fee Related DE60037886T2 (de) 1999-05-07 2000-05-04 Verfahren für die Reinigung von rekombinanten Wachstumshormonantagonist-Proteinen unter Verwendung von Zwei-Phasen-flüssig-flüssig-Extraktionsverfahrensweisen

Country Status (13)

Country Link
US (1) US6437101B1 (de)
EP (1) EP1194445B1 (de)
JP (1) JP2002544139A (de)
AT (1) ATE384737T1 (de)
AU (1) AU777974B2 (de)
BR (1) BR0010707A (de)
CA (1) CA2372513A1 (de)
DE (1) DE60037886T2 (de)
ES (1) ES2303507T3 (de)
IL (1) IL146381A0 (de)
MX (1) MXPA01011356A (de)
NZ (1) NZ515381A (de)
WO (1) WO2000068260A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6966992B2 (en) * 2001-03-19 2005-11-22 Akzo Nobel Nv Method of purification of molecules using unbranched terminal alkyldiols
BR0314619A (pt) * 2002-09-18 2005-08-02 Univ Montreal Ct Hospitalier Chum Análogos de ghrh
ATE378349T1 (de) * 2004-06-25 2007-11-15 Kuehni Ag Verfahren zur extraktion von biologischem material
EP1761552B1 (de) 2004-06-29 2008-09-17 Ares Trading S.A. Verfahren zur aufreinigung von il-18-bindendem protein
US20070048786A1 (en) * 2005-09-01 2007-03-01 Arnon Chait Systems and methods for fractionation of protein mixtures
US20080070230A1 (en) * 2006-06-12 2008-03-20 Wyeth Methods for reducing or preventing liquid-liquid phase separation in high concentration protein solutions
KR100836171B1 (ko) * 2007-01-19 2008-06-09 인하대학교 산학협력단 분배 향상 물질이 접합된 생물물질을 이용한 수성이상계추출법에서의 친화성 분리 방법
DE102008013490A1 (de) * 2008-03-10 2009-09-17 Bayer Technology Services Gmbh Verfahren zur Reinigung therapeutischer Proteine
CN102224160A (zh) * 2008-11-25 2011-10-19 通用电气健康护理生物科学股份公司 用于纯化治疗蛋白的水性两相萃取加强的沉淀方法
DE102011001743A1 (de) * 2011-04-01 2012-10-04 Technische Universität Dortmund Verfahren zur Trennung/Reinigung von Biomolekühlen
CN102662015B (zh) * 2012-04-12 2013-11-20 湖南师范大学 基于无机盐促相变分离纳米金的生物样品的净化方法
US20130310554A1 (en) * 2012-05-18 2013-11-21 Hong Kong Baptist University Aqueous Diphase Solvent System for Preparing High-Purity Polysaccharides
CN111249531B (zh) * 2020-01-19 2022-03-22 巴斯特医药科技（常州）有限公司 一种高活性植物酶制备胶原蛋白支架的方法
CN114957494A (zh) * 2021-02-23 2022-08-30 江苏农牧科技职业学院 一种采用双水相萃取黄精多糖中蛋白质的工艺

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK144731C (da) 1976-07-30 1982-10-18 Nordisk Insulinlab Fremgangsmaade til isolering af proteinhormoner stammende fra humant hypofysevaev
JPS5815989A (ja) * 1981-06-12 1983-01-29 Sanki Eng Kk 生理活性蛋白質の精製方法
DE3908422A1 (de) * 1989-03-15 1990-09-20 Forschungszentrum Juelich Gmbh Verfahren zur extraktion von proteinen aus waessrigen biomassesuspensionen
US5139943A (en) * 1989-06-13 1992-08-18 Genencor International, Inc. Processes for the recovery of microbially produced chymosin
US5151358A (en) 1989-06-13 1992-09-29 Genencor International, Inc. Processes for the recovery of naturally produced chymosin
US5182369A (en) 1990-02-28 1993-01-26 Monsanto Company Method for purifying somatotropin monomers
US5328841A (en) * 1990-10-05 1994-07-12 Genencor International, Inc. Methods for isolating EG III cellulase component and EG III cellulase in polyethylene glycol using inorganic salt and polyethylene glycol
SE9003534D0 (sv) * 1990-11-06 1990-11-06 Kabigen Ab A method for isolating and purifying peptides and proteins
US5407810A (en) 1993-08-20 1995-04-18 Genentech, Inc. Aqueous multiple-phase isolation of polypeptide
US5734024A (en) * 1996-04-19 1998-03-31 Boris Y. Zaslavsky Method for determining the biological activity of recombinant human growth hormone
GB0205898D0 (en) * 2002-03-13 2002-04-24 Pharmacia Ab Treatment of type I diabetes mellitus

Also Published As

Publication number Publication date
EP1194445B1 (de) 2008-01-23
DE60037886T2 (de) 2009-01-22
EP1194445A4 (de) 2002-10-09
ES2303507T3 (es) 2008-08-16
CA2372513A1 (en) 2000-11-16
NZ515381A (en) 2003-10-31
AU4703600A (en) 2000-11-21
BR0010707A (pt) 2002-06-04
EP1194445A1 (de) 2002-04-10
ATE384737T1 (de) 2008-02-15
AU777974B2 (en) 2004-11-04
JP2002544139A (ja) 2002-12-24
US6437101B1 (en) 2002-08-20
MXPA01011356A (es) 2003-08-20
IL146381A0 (en) 2002-07-25
WO2000068260A1 (en) 2000-11-16

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee