DE60014166D1 - Verfahren zum messen der deacetylaseaktivität und verfahren zum screenen von inhibitoren oder promotoren dieses enzyms - Google Patents

Verfahren zum messen der deacetylaseaktivität und verfahren zum screenen von inhibitoren oder promotoren dieses enzyms

Info

Publication number
DE60014166D1
DE60014166D1 DE60014166T DE60014166T DE60014166D1 DE 60014166 D1 DE60014166 D1 DE 60014166D1 DE 60014166 T DE60014166 T DE 60014166T DE 60014166 T DE60014166 T DE 60014166T DE 60014166 D1 DE60014166 D1 DE 60014166D1
Authority
DE
Germany
Prior art keywords
promotors
enzyme
deacetylase activity
screening inhibitors
measuring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60014166T
Other languages
English (en)
Other versions
DE60014166T2 (de
Inventor
Katsuyuki Tamai
Toshiaki Miyazaki
Emi Wada
Ayumi Tatsuzawa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CycLex Co Ltd
Original Assignee
CycLex Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CycLex Co Ltd filed Critical CycLex Co Ltd
Application granted granted Critical
Publication of DE60014166D1 publication Critical patent/DE60014166D1/de
Publication of DE60014166T2 publication Critical patent/DE60014166T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60014166T 1999-11-29 2000-11-29 Verfahren zum messen der deacetylaseaktivität und verfahren zum screenen von inhibitoren oder promotoren dieses enzyms Expired - Lifetime DE60014166T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP33856599 1999-11-29
JP33856599A JP2001149081A (ja) 1999-11-29 1999-11-29 脱アセチル化酵素の活性測定方法、並びにこれら酵素の阻害剤もしくは促進剤のスクリーニング方法
PCT/JP2000/008417 WO2001040506A1 (fr) 1999-11-29 2000-11-29 Procede pour mesurer l'activite des desacetylases et procede pour cribler un inhibiteur ou un promoteur de ces enzymes

Publications (2)

Publication Number Publication Date
DE60014166D1 true DE60014166D1 (de) 2004-10-28
DE60014166T2 DE60014166T2 (de) 2005-10-06

Family

ID=18319382

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60014166T Expired - Lifetime DE60014166T2 (de) 1999-11-29 2000-11-29 Verfahren zum messen der deacetylaseaktivität und verfahren zum screenen von inhibitoren oder promotoren dieses enzyms

Country Status (6)

Country Link
US (2) US7033778B2 (de)
EP (1) EP1243658B1 (de)
JP (1) JP2001149081A (de)
CA (1) CA2392711C (de)
DE (1) DE60014166T2 (de)
WO (1) WO2001040506A1 (de)

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JP2001149081A (ja) * 1999-11-29 2001-06-05 Cyclex Co Ltd 脱アセチル化酵素の活性測定方法、並びにこれら酵素の阻害剤もしくは促進剤のスクリーニング方法
US7452664B2 (en) * 1999-12-15 2008-11-18 Massachusetts Institute Of Technology Methods for identifying agents which alter histone protein acetylation
US8642284B1 (en) 1999-12-15 2014-02-04 Massachusetts Institute Of Technology Methods for identifying agents that alter NAD-dependent deacetylation activity of a SIR2 protein
US7572575B2 (en) 2000-12-13 2009-08-11 Massachusetts Institute Of Technology SIR2 activity
AU2003215112A1 (en) * 2002-02-07 2003-09-02 Axys Pharmaceuticals Novel bicyclic hydroxamates as inhibitors of histone deacetylase
CA2484013C (en) * 2002-05-23 2011-09-20 The Regents Of The University Of California Methods of modulating mitochondrial nad-dependent deacetylase
EP1403639A1 (de) * 2002-09-30 2004-03-31 G2M Cancer Drugs AG Antikörper als Diagnosemittel in der medizinischen Therapie mit Histondeacetylase-Hemmern
US7250514B1 (en) 2002-10-21 2007-07-31 Takeda San Diego, Inc. Histone deacetylase inhibitors
US7375228B2 (en) * 2003-03-17 2008-05-20 Takeda San Diego, Inc. Histone deacetylase inhibitors
GB0307428D0 (en) * 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
WO2005002527A2 (en) * 2003-07-03 2005-01-13 Massachusetts Institute Of Technology Sirt1 modulation of adipogenesis and adipose function
US20050159470A1 (en) * 2003-12-19 2005-07-21 Syrrx, Inc. Histone deacetylase inhibitors
WO2005066151A2 (en) * 2003-12-19 2005-07-21 Takeda San Diego, Inc. Histone deacetylase inhibitors
WO2005062952A2 (en) 2003-12-23 2005-07-14 The Regents Of The University Of California Compositions and methods for modulating sirtuin activity
EP1557474A1 (de) * 2004-01-21 2005-07-27 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO Methode zur Bestimmung einer Protein-modifizierenden bzw. -demodifizierenden Aktivität und hierfür geeignete Materialien
GB0403041D0 (en) * 2004-02-11 2004-03-17 Milner Anne J Induction of apoptosis
JP2008524246A (ja) * 2004-12-16 2008-07-10 タケダ サン ディエゴ インコーポレイテッド ヒストンデアセチラーゼ阻害剤
WO2006094239A2 (en) * 2005-03-03 2006-09-08 Sirtris Pharmaceuticals, Inc. Fluorescence polarization assays for acetyltransferase/deacetylase activity
US20070099830A1 (en) 2005-04-21 2007-05-03 Massachusetts Institute Of Technology Sirt4 activities
JP2008540574A (ja) * 2005-05-11 2008-11-20 タケダ サン ディエゴ インコーポレイテッド ヒストンデアセチラーゼ阻害剤
CN101263121A (zh) * 2005-07-14 2008-09-10 塔克达圣地亚哥公司 组蛋白脱乙酰基酶抑制剂
GB0518237D0 (en) * 2005-09-07 2005-10-19 Angeletti P Ist Richerche Bio Therapeutic compounds
ES2389111T3 (es) * 2005-12-02 2012-10-23 Sirtris Pharmaceuticals, Inc. Ensayos de espectrometría de masas para la actividad acetiltransferasa/desacetilasa
US20070248590A1 (en) * 2005-12-02 2007-10-25 Sirtris Pharmaceuticals, Inc. Modulators of CDC2-like kinases (CLKS) and methods of use thereof
JP2009525955A (ja) * 2006-01-13 2009-07-16 タケダ サン ディエゴ インコーポレイテッド ヒストンデアセチラーゼ阻害剤
CN101008009B (zh) * 2006-01-24 2010-04-07 中国人民解放军军事医学科学院基础医学研究所 用于快速筛选组蛋白去乙酰化酶抑制剂的细胞模型
DE602007013213D1 (de) * 2006-06-06 2011-04-28 Massachusetts Inst Technology Cholesterinregulierender komplex aus sirt1 und lxr und seine verwendung
US8518635B2 (en) 2006-06-12 2013-08-27 The J. David Gladstone Institutes Regulation of protein activity by reversible acetylation
JP2008289437A (ja) 2007-05-28 2008-12-04 Sony Corp レーザーを用いた酵素活性測定方法及び酵素活性測定装置
US20100160262A1 (en) * 2008-12-23 2010-06-24 Melanie Ott Compositions and methods for modulating sirtuin activity
CA2779497A1 (en) 2009-10-30 2011-05-05 Massachusetts Institute Of Technology The use of ci-994 and dinaline for the treatment of memory/cognition and anxiety disorders
JP5794659B2 (ja) * 2010-04-19 2015-10-14 国立大学法人九州工業大学 ヒストンメチル化酵素活性の測定方法
WO2012033993A2 (en) 2010-09-10 2012-03-15 Promega Corporation Methods for assaying enzyme activities
US9637773B2 (en) 2011-01-13 2017-05-02 Enzo Life Sciences, Inc. Compounds and methods for detection of enzymes that remove formyl, succinyl, methyl succinyl or myristoyl groups from ε-amino lysine moieties
WO2012131345A2 (en) * 2011-03-25 2012-10-04 Almac Sciences (Scotland) Limited Enzyme assays
WO2013134261A1 (en) * 2012-03-05 2013-09-12 President And Fellows Of Harvard College Systems and methods for epigenetic sequencing
CA2875715C (en) * 2012-06-05 2023-03-21 Mcmaster University Screening method and systems utilizing mass spectral fragmentation patterns
US10087485B2 (en) * 2012-06-06 2018-10-02 The Trustees Of Princeton University DNA barcoding of designer mononucleosome and chromatin array libraries for the profiling of chromatin readers, writers, erasers, and modulators thereof
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
EP3283629A4 (de) 2015-04-17 2018-08-29 President and Fellows of Harvard College Barcodierungssysteme und verfahren für die gensequenzierung und andere anwendungen
CN105294475A (zh) * 2015-11-14 2016-02-03 复旦大学 广谱的去乙酰化酶抑制剂乙酰化赖氨酸及其应用
CN110082327A (zh) * 2019-04-30 2019-08-02 阜阳师范学院 一种***h3k27乙酰化指数分析方法

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* Cited by examiner, † Cited by third party
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JPH0755942B2 (ja) * 1985-07-29 1995-06-14 味の素株式会社 酵素活性測定用ペプチド誘導体及びその使用法
JPH05137599A (ja) 1991-11-22 1993-06-01 Takara Shuzo Co Ltd 蛍光基質
JPH0856665A (ja) 1994-07-07 1996-03-05 Nederland Centr Org Toegepast Natuur Onder タンパク質分解酵素用の基質としての修飾された酵素前駆体
EP0691409B1 (de) * 1994-07-07 2002-10-23 Nederlandse Organisatie voor toegepast-natuurwetenschappelijk Onderzoek TNO Modifizierte Proenzyme als Substrate für proteolische Enzyme
JP3207120B2 (ja) 1996-07-24 2001-09-10 株式会社分子バイオホトニクス研究所 酵素活性測定方法
JPH10313896A (ja) 1997-05-15 1998-12-02 Yamanouchi Pharmaceut Co Ltd マトリックスメタロプロテアーゼに対する発色団又は蛍光団を有する新規な活性測定用合成基質
EP1050581A4 (de) * 1998-01-20 2005-10-19 Med & Biological Lab Co Ltd Verfahren zur detektion von acetyltransferase- und deacetylaseaktivitäten und verfahren zum auffinden von hemmstoffen oder beschleunigern dieser enzyme
US6884597B1 (en) * 1998-01-20 2005-04-26 Medical & Biological Laboratories, Co., Ltd. Method for detecting acetyltransferase and deacetylase activities and method for screening inhibitors or enhancers of these enzymes
JPH11318495A (ja) * 1998-05-11 1999-11-24 Igaku Seibutsugaku Kenkyusho:Kk 抗アセチル化リジン抗体およびその利用
DE60041542D1 (de) * 1999-08-20 2009-03-26 Univ Texas Hdac4- und hdac5-abhängige regulation der genexpression im herzen
JP2001149081A (ja) * 1999-11-29 2001-06-05 Cyclex Co Ltd 脱アセチル化酵素の活性測定方法、並びにこれら酵素の阻害剤もしくは促進剤のスクリーニング方法
AU2001298014A1 (en) * 2000-03-24 2003-01-29 Methylgene, Inc. Inhibition of specific histone deacetylase isoforms
US6706686B2 (en) * 2001-09-27 2004-03-16 The Regents Of The University Of Colorado Inhibition of histone deacetylase as a treatment for cardiac hypertrophy

Also Published As

Publication number Publication date
CA2392711C (en) 2011-01-04
EP1243658B1 (de) 2004-09-22
CA2392711A1 (en) 2001-06-07
DE60014166T2 (de) 2005-10-06
US20030082668A1 (en) 2003-05-01
JP2001149081A (ja) 2001-06-05
EP1243658A4 (de) 2003-07-23
US7033778B2 (en) 2006-04-25
US7256013B2 (en) 2007-08-14
WO2001040506A1 (fr) 2001-06-07
US20060040336A1 (en) 2006-02-23
EP1243658A1 (de) 2002-09-25

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