DE3687112D1 - Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien. - Google Patents

Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien.

Info

Publication number
DE3687112D1
DE3687112D1 DE8686850415T DE3687112T DE3687112D1 DE 3687112 D1 DE3687112 D1 DE 3687112D1 DE 8686850415 T DE8686850415 T DE 8686850415T DE 3687112 T DE3687112 T DE 3687112T DE 3687112 D1 DE3687112 D1 DE 3687112D1
Authority
DE
Germany
Prior art keywords
gram
sub
recombinant dna
structural gene
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE8686850415T
Other languages
English (en)
Other versions
DE3687112T2 (de
Inventor
Lars Abrahamsen
Tomas Moks
Bjoern Nilsson
Mathias Uhlen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Health AB
Original Assignee
Kabigen AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabigen AB filed Critical Kabigen AB
Publication of DE3687112D1 publication Critical patent/DE3687112D1/de
Application granted granted Critical
Publication of DE3687112T2 publication Critical patent/DE3687112T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
DE8686850415T 1985-12-13 1986-12-02 Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien. Expired - Fee Related DE3687112T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE8505921A SE8505921D0 (sv) 1985-12-13 1985-12-13 A method to export gene products to the growth medium of gram negative bacteria

Publications (2)

Publication Number Publication Date
DE3687112D1 true DE3687112D1 (de) 1992-12-17
DE3687112T2 DE3687112T2 (de) 1993-06-03

Family

ID=20362467

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8686850415T Expired - Fee Related DE3687112T2 (de) 1985-12-13 1986-12-02 Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien.

Country Status (11)

Country Link
US (1) US5156959A (de)
EP (1) EP0225860B1 (de)
JP (2) JPH082314B2 (de)
AT (1) ATE82320T1 (de)
AU (1) AU597317B2 (de)
CA (1) CA1314010C (de)
DE (1) DE3687112T2 (de)
DK (1) DK175020B1 (de)
ES (1) ES2052497T3 (de)
GR (1) GR3006597T3 (de)
SE (1) SE8505921D0 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE8601940D0 (sv) * 1986-04-25 1986-04-25 Kabigen Ab Preparation of fused proteins, antibodies and processes therefor
WO1990000200A1 (en) * 1988-06-27 1990-01-11 Genex Corporation Thermal release of recombinant protein into culture media
EP0449839A1 (de) * 1988-09-30 1991-10-09 AlliedSignal Inc. Bakterielle stämme zur heterologen genexpression
WO1993002107A1 (en) * 1991-07-25 1993-02-04 Oriental Yeast Co., Ltd. Immunoglobulin-combining artificial protein
DE69403654T2 (de) * 1993-03-29 1997-12-11 Du Pont Ein verfahren zur erhöhung der produktion von biologisch aktiven rekombinanten proteinen
US6958148B1 (en) 1998-01-20 2005-10-25 Pericor Science, Inc. Linkage of agents to body tissue using microparticles and transglutaminase
KR100312456B1 (ko) 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
JP3553858B2 (ja) 1999-08-25 2004-08-11 東洋紡績株式会社 血管網類似構造体を有する細胞培養用モジュール
JP4220756B2 (ja) 2002-10-28 2009-02-04 極東製薬工業株式会社 培養器、培養器の製造方法及び培養方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
NO172595C (no) * 1983-02-07 1993-08-11 Battelle Memorial Institute Fremgangsmaate og ekspresjonsplasmid for fremstilling av hemagglutinin under kontroll av drosophila hsp-promoter
SE8300693L (sv) * 1983-02-09 1984-08-10 Sven Lofdahl Sett att framstella och isolera proteiner och polypeptider samt en hybridvektor for detta
US4472302A (en) * 1983-03-09 1984-09-18 Merck & Co., Inc. Heat shock process for the isolation of bacterial protein
US4624922A (en) * 1983-12-09 1986-11-25 Rikagaku Kenkyusho Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
FR2556740B1 (fr) * 1983-12-14 1987-02-27 Merieux Inst Nouveaux mutants d'escherichia coli, capables d'excreter dans le milieu extracellulaire des proteines periplasmiques, leur obtention et leur application a la preparation de beta-lactamases ou de proteines hybrides de beta-lactamases
SE8505922D0 (sv) * 1985-12-13 1985-12-13 Kabigen Ab Construction of an igg binding protein to facilitate downstream processing using protein engineering

Also Published As

Publication number Publication date
CA1314010C (en) 1993-03-02
DK600386D0 (da) 1986-12-12
EP0225860A3 (en) 1988-10-19
EP0225860A2 (de) 1987-06-16
JPH082314B2 (ja) 1996-01-17
DK175020B1 (da) 2004-04-26
JP2504723B2 (ja) 1996-06-05
AU6633286A (en) 1987-06-18
US5156959A (en) 1992-10-20
ATE82320T1 (de) 1992-11-15
JPS62190088A (ja) 1987-08-20
GR3006597T3 (de) 1993-06-30
EP0225860B1 (de) 1992-11-11
SE8505921D0 (sv) 1985-12-13
JPH0746988A (ja) 1995-02-21
AU597317B2 (en) 1990-05-31
DE3687112T2 (de) 1993-06-03
DK600386A (da) 1987-06-14
ES2052497T3 (es) 1994-07-16

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: PHARMACIA & UPJOHN AB, STOCKHOLM, SE

8339 Ceased/non-payment of the annual fee