DE2839433A1 - STANDARD AQUEOUS LIPID SOLUTION AND METHOD FOR THEIR PRODUCTION - Google Patents
STANDARD AQUEOUS LIPID SOLUTION AND METHOD FOR THEIR PRODUCTIONInfo
- Publication number
- DE2839433A1 DE2839433A1 DE19782839433 DE2839433A DE2839433A1 DE 2839433 A1 DE2839433 A1 DE 2839433A1 DE 19782839433 DE19782839433 DE 19782839433 DE 2839433 A DE2839433 A DE 2839433A DE 2839433 A1 DE2839433 A1 DE 2839433A1
- Authority
- DE
- Germany
- Prior art keywords
- lipid
- standard solution
- aqueous
- cholesterol
- detergents
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/04—Endocrine or metabolic disorders
- G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/104165—Lipid, cholesterol, or triglyceride standard or control
Description
Merck Patent Gesellschaft 8. September 1978Merck Patent Society September 8, 1978
mit beschränkter Haftung Darmstadtwith limited liability Darmstadt
Wäßrige Lipid-Standardlösung und Verfahren zu ihrer HerstellungAqueous lipid standard solution and process for its preparation
030012/0392030012/0392
Wäßrige Lipid-Standardlösung und Verfahren zu ihrer HerstellungAqueous lipid standard solution and process for its preparation
Die Erfindung betrifft eine wäßrige Lipid-Standardlösung, die neben einem Lipid ein ionisches und ein nichtionisches Detergens enthält/ sowie ein Verfahren zu ihrer Herstellung.The invention relates to an aqueous lipid standard solution which, in addition to a lipid, has an ionic and a nonionic Contains detergent and a process for its manufacture.
Für klinisch-chemische Messungen werden in der Regel Standardlösungen benötigt, deren Eigenschaften möglichst denen eines Serums ähneln sollen. Dazu muß die betreffende Substanz in einem wäßrigen System gelöst werden, da sich nur so die Standardprobe wie ein Serum verhält. Eine solche Standardlösung sollte außerdem ausreichend stabil sein und sich über einen längeren Zeitraum hinweg nicht verändern. Diese Forderungen sind besonders schwer zu erfüllen bei Standardlösungen für die Bestimmung von Lipiden, z.B. von Cholesterin oder von Triglyceriden. Für Cholesterin sind in der Literatur außer der Verwendung von organischen Lösungsmitteln folgende wäßrige Lösungsmittelsysteme beschrieben: Nichtionische Detergentien (DAS 15 23 133, Ärztl. Lab. 20, 148 - 151 (1974)) und Wasser-Alkohol-Mischungen mit einem Zusatz an Detergentien (DAS 23 24 386). Standardlösungen, die mit nichtionischen Detergentien hergestellt werden, sind vor allem bei Cholesterinkonzentrationen im pathologischen Bereich nur wenige WochenFor clinical-chemical measurements, standard solutions are usually required, whose properties are as good as possible should resemble those of a serum. For this purpose, the substance in question must be dissolved in an aqueous system, since just so the standard sample behaves like a serum. Such a standard solution should also be sufficiently stable and do not change over a longer period of time. These requirements are particularly difficult to meet with standard solutions for the determination of lipids, e.g. cholesterol or triglycerides. For cholesterol In addition to the use of organic solvents, the following aqueous solvent systems are described in the literature: Nonionic detergents (DAS 15 23 133, Ärztl. Lab. 20, 148-151 (1974)) and water-alcohol mixtures with an addition of detergents (DAS 23 24 386). Standard solutions made with nonionic detergents are produced are only a few weeks, especially with cholesterol concentrations in the pathological range
03001?03001?
stabil; Standardlösungen mit Alkoholzusatz verdunsten wegen ihres Gehaltes an Alkoholen wesentlich stärker als eine Serumprobe, selbst bei Verwendung höher siedender Alkohole. Eine Senkung des Alkoholgehaltes führt zur Ausfällung des Lipids; die gleichzeitige Erhöhung der Detergenskonzentration resultiert in einer unerwünscht hohen Viskosität.stable; Evaporate standard solutions with added alcohol because of its alcohol content, it is much stronger than a serum sample, even if higher boiling points are used Alcohols. Lowering the alcohol content leads to the precipitation of the lipid; the simultaneous increase the detergent concentration results in an undesirably high viscosity.
Der Erfindung liegt die Aufgabe zugrunde, eine alkoholfreie, stabile, wäßrige Lipid-Standardlösung zur Verfügung zu stellen, die einem Serum möglichst nahe kommt, beim unvermeidlichen Stehen der Proben möglichst wenig verdunstet und deren Viskosität die Dosierung nicht beeinflußt. The invention is based on the object of providing an alcohol-free, stable, aqueous lipid standard solution that comes as close as possible to a serum and evaporates as little as possible when the samples are inevitable and whose viscosity does not affect the dosage.
Erfindungsgemäß wurde die Aufgabe durch eine wäßrige, alkoholfreie Lipid-Standardlösung gelöst, die neben einem Lipid ein nichtionisches und ein ionisches Detergens enthält. According to the invention, the object was achieved by an aqueous, alcohol-free lipid standard solution which, in addition to a Lipid contains a nonionic and an ionic detergent.
Gegenstand der Erfindung sind wäßrige Lipid-Standardlösungen, enthaltend mindestens ein Lipid und ein nichtionisches Detergens, die gekennzeichnet sind durch einen Gehalt von 0,5 - 10 Gew.-% eines ionischen Detergens.The invention relates to aqueous lipid standard solutions containing at least one lipid and one nonionic Detergents which are characterized by a content of 0.5-10% by weight of an ionic detergent.
Überraschenderweise hat sich gezeigt, daß es möglich ist, eine alkoholfreie, stabile, wäßrige Standardlösung herzustellen, wenn man zusätzlich zu einem der in den oben angegebenen Publikationen genannten nichtionischen Detergentien ein ionisches Detergens in der relativ hohen Konzentration von 0,5 - 10 Gew.-% verwendet. Das Lösungsvermögen dieser Mischungen ist dann sogar so gut, daß auch Cholesterinester langkettiger Fettsäuren, die die überwiegende Form des Cholesterins im Serum sind, in Lösung gehalten werden können, obwohl dieseSurprisingly, it has been shown that it is possible to produce an alcohol-free, stable, aqueous standard solution if, in addition to one of the in the publications mentioned above nonionic detergents an ionic detergent in the relative high concentration of 0.5-10% by weight. The solvency of these mixtures is then even so good that even cholesterol esters of long-chain fatty acids, which are the predominant form of cholesterol in serum can be kept in solution, although this
w .IG (70D) ölw .IG (70D) oil
030012/0392030012/0392
wesentlich unpolarer und schlechter in Wasser löslich sind als freies Cholesterin. Auch ist es damit möglich, andere Lipide, deren Bestimmung klinische Bedeutung haben, wie Triglyceride oder Lecithin, in diese Lösung einzubringen.are much less polar and less soluble in water than free cholesterol. It is also possible with it other lipids, the determination of which are of clinical importance, such as triglycerides or lecithin, in this solution bring in.
Die erfindungsgemäße wäßrige Lipid-Standardlösung enthält neben 0,2-4 g/l Cholesterin und/oder 0,1-2 g/l Triolein etwa 2-20 Gew.-%, vorzugsweise 5-15 Gew.-%, nichtionisches Detergens und einen zusätzlichen Gehalt von etwa 0,5 - 10 Gew.-%, vorzugsweise 1-5 Gew.-%, ionisches Detergens.The aqueous lipid standard solution according to the invention In addition to 0.2-4 g / l cholesterol and / or 0.1-2 g / l triolein, it contains about 2-20% by weight, preferably 5-15% by weight, nonionic detergent and an additional content of about 0.5-10% by weight, preferably 1-5% by weight, ionic detergent.
Als nichtionische Detergentien kommen z.B. PolyäthylenglykolmonoalkyIphenyläther, Diäthylenglykolmonobutyläther, Oxypolyäthoxydodecan, Octylphenoxypolyäthoxyäthanol, Polyäthoxyäthylensorbitanfettsäureester in Frage, vorzugsweise Polyäthylenglykolmonoalkylphenyläther.Polyethylene glycol monoalkyIphenyl ether, for example, can be used as nonionic detergents. Diethylene glycol monobutyl ether, Oxypolyäthoxydodecan, Octylphenoxypolyäthoxyäthanol, Polyethoxyethylene sorbitan fatty acid ester in question, preferably polyethylene glycol monoalkylphenyl ether.
Geeignete ionische Detergentien sind kationische Detergentien wie Alkylbenzyldimethylammoniumchlorid, Alkylbenzyltrimethylammoniumchlorid, Methylbenzethoniumchlorid. Geeignet sind weiterhin anionische Detergentien wie Natriumdioctylsulfosuccinat, Natriumtriisopropylnaphthalinsulfonat, Natriumdodecylsulfat, Natrium-N-lauroyisarkosinat. Suitable ionic detergents are cationic detergents such as alkylbenzyldimethylammonium chloride, Alkylbenzyltrimethylammonium chloride, methylbenzethonium chloride. Anionic detergents are also suitable such as sodium dioctyl sulfosuccinate, sodium triisopropyl naphthalene sulfonate, sodium dodecyl sulfate, sodium N-lauroyis sarcosinate.
Die Standardlösung kann zusätzlich weitere Lipide enthalten, die klinisch bedeutsam sind, wie Cholesterinester langkettiger Fettsäuren (0,1 - 2 g/l) und/oder Lecithin (0,2 - 4 g/l). Gegebenenfalls kann der pH-Wert der Standardlösung im Bereich von 6-8 mit Hilfe eines Puffers eingestellt werden. Als geeignete Puffer haben sich z.B. Tris-, Phosphat-, Triethanolamin- oder Imidazol-Puffer in einer Konzentration von 10 - 100 mmol/1 erwiesen,The standard solution can also contain other lipids that are clinically important, such as cholesterol esters long-chain fatty acids (0.1 - 2 g / l) and / or lecithin (0.2 - 4 g / l). Optionally, the pH can of the standard solution can be set in the range of 6-8 with the help of a buffer. Have as suitable buffers e.g. Tris, phosphate, triethanolamine or imidazole buffers proven in a concentration of 10 - 100 mmol / 1,
5029 (70°>) öl5029 (70 °>) oil
030012/039030012/039
- sr -- sr -
bevorzugt wird ein Tris-HCl-Puffer vom pH-Wert 7,4 in einer Konzentration von 20 mmol/1 verwendet. Zur Vermeidung mikrobieller Kontamination können noch 0,5 - 20 g/l eines Alkaliazids zugesetzt werden, vorzugsweise 1 g/l Natriumazid. a Tris-HCl buffer with a pH of 7.4 in is preferred a concentration of 20 mmol / 1 is used. To avoid microbial contamination, 0.5 - 20 g / l of an alkali azide can be added, preferably 1 g / l sodium azide.
Die Herstellung bekannter Lipid-Standardlösungen oder -Emulsionen erfolgt durch Ultraschallbehandlung oder durch vorsichtiges und langsames Verdünnen einer Mischung aus Cholesterin und Detergens mit Wasser. Ersteres erfordert aufwendige technische Apparaturen und arbeitstechnische Sicherheitsmaßnahmen, letzteres eine erhebliche Sorgfalt beim Zufügen des Wassers, um eine Ausfällung des Cholesterins zu vermeiden. Diese Nachteile lassen sich umgehen, wenn man die neue Lipid-Standardlösung erfindungsgemäß in der Weise herstellt, daß man die Lipide zusammen mit den Detergentien in einem leicht flüchtigen organischen Lösungsmittel, z.B. Dichlormethan oder Methanol, löst und das Lösungsmittel unter vermindertem Druck abdampft. Dadurch wird eine gute Durchmischung der Lipide und der Detergentien erreicht. Anschließend wird die benötigte Menge Wasser, gegebenenfalls unter leichtem Erwärmen, hinzugegeben. Das Wasser kann zusätzlich ein Alkaliazid und einen Puffer enthalten.The preparation of known lipid standard solutions or -Emulsions are made by ultrasonic treatment or by carefully and slowly diluting a mixture Cholesterol and detergent with water. The former requires complex technical and work-related equipment Precautions, the latter take considerable care when adding the water to prevent precipitation of the cholesterol to avoid. These disadvantages can be avoided if the new lipid standard solution according to the invention in the Way that you can prepare the lipids together with the detergents in a volatile organic Solvent, e.g. dichloromethane or methanol, dissolves and the solvent evaporates under reduced pressure. Through this a good mixing of the lipids and detergents is achieved. Then the required amount Water, if necessary with slight warming, added. The water can also contain an alkali azide and a Buffers included.
Die Erfindung wird durch die folgenden Beispiele näher erläutert.The invention is illustrated in more detail by the following examples.
6660 (709) öl6660 (709) oil
0012/03i-20012 / 03i-2
Beispiel 1 -.Example 1 -.
In 25 ml Dichlormethan werdenBe in 25 ml of dichloromethane
300 mg Cholesterin,300 mg cholesterol,
200 mg Cholesterinlinolat,200 mg cholesterol linolate,
200 mg Triolein,200 mg triolein,
100 mg Lecithin,100 mg lecithin,
5 g Polyäthylenglykol-mono-/p-(l,l,3,3-tetramethylbutyl)-phenyl?-äther und5 g polyethylene glycol mono- / p- (l, l, 3,3-tetramethylbutyl) -phenyl? -Ether and
1 g Natriumdioctylsulfosuccinat1 g sodium dioctyl sulfosuccinate
gelöst. Das Dichlormethan wird unter vermindertem Druck abgedampft und der Rückstand mit 80 ml Wasser von ca 40° C aufgenommen; nach dem Erkalten wird mit Wasser auf 100 ml aufgefüllt. Man erhält eine klare, stabile Lipid-Standardlösung. solved. The dichloromethane is evaporated off under reduced pressure and the residue was taken up with 80 ml of water at approx. 40 ° C; after cooling, make up to 100 ml with water filled up. A clear, stable lipid standard solution is obtained.
In 25 ml Dichlormethan werdenBe in 25 ml of dichloromethane
300 mg Cholesterin, 5 g Polyäthylenglykolmonooctylphenyläther und 1 g Natriumdodecylsulfat300 mg cholesterol, 5 g polyethylene glycol monooctylphenyl ether and 1 g sodium dodecyl sulfate
gelöst. Das Dichlormethan wird unter vermindertem Druck abgedampft und der Rückstand wie in Beispiel 1 beschrieben mit Wasser aufgenommen und aufgefüllt.solved. The dichloromethane is evaporated off under reduced pressure and the residue was taken up with water as described in Example 1 and made up.
In 25 ml Methanol werdenBe in 25 ml of methanol
300 mg Cholesterin, 200 mg Cholesterinlinolat, 200 mg Triolein,300 mg cholesterol, 200 mg cholesterol linolate, 200 mg triolein,
10 g Polyäthylenglykolmonooctylphenyläther und 5 g Alkylbenzyldimßthylammoniumchlorid10 g of polyethylene glycol monooctylphenyl ether and 5 g of alkylbenzyldimethylammonium chloride
(7θ'ΐ) öl(7θ'ΐ) oil
030012/0392030012/0392
gelöst. Der Alkohol wird unter vermindertem Druck abgedampft und der Rückstand wie in Beispiel 1 beschrieben mit Wasser aufgenommen und aufgefüllt.solved. The alcohol is evaporated off under reduced pressure and the residue was taken up with water as described in Example 1 and made up.
Beispiel 4
In 25 ml Methanol werdenExample 4
Be in 25 ml of methanol
300 mg Cholesterin, 5 g Polyäthylenglykolmonooctylphenyläther und 2 g Methylbenzethoniumchlorid300 mg cholesterol, 5 g polyethylene glycol monooctylphenyl ether and 2 g methylbenzethonium chloride
gelöst. Der Alkohol wird unter vermindertem Druck abgedampft und der Rückstand wird mit warmem Tris-HCl-Puffer (20 mmol/1; pH 7,4), der zusätzlich 1 g/l Natriumazid enthält, aufgenommen und nach dem Erkalten mit dieser Lösung auf 100 ml aufgefüllt.solved. The alcohol is evaporated under reduced pressure and the residue is washed with warm Tris-HCl buffer (20 mmol / 1; pH 7.4), which additionally contains 1 g / l sodium azide, taken up and made up to 100 ml with this solution after cooling.
4 312 (709) öl4,312 (709) oil
Ö30012/03Ö2Ö30012 / 03Ö2
Claims (3)
mit beschränkter Haftung
DarmstadtMerck Patent Society
with limited liability
Darmstadt
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19782839433 DE2839433A1 (en) | 1978-09-11 | 1978-09-11 | STANDARD AQUEOUS LIPID SOLUTION AND METHOD FOR THEIR PRODUCTION |
| EP79103070A EP0009596B1 (en) | 1978-09-11 | 1979-08-21 | Aqueous lipid standard solution |
| DE7979103070T DE2964278D1 (en) | 1978-09-11 | 1979-08-21 | Aqueous lipid standard solution |
| JP11530279A JPS5539095A (en) | 1978-09-11 | 1979-09-10 | Standard fat solution |
| US06/074,457 US4289649A (en) | 1978-09-11 | 1979-09-11 | Aqueous lipid standard solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19782839433 DE2839433A1 (en) | 1978-09-11 | 1978-09-11 | STANDARD AQUEOUS LIPID SOLUTION AND METHOD FOR THEIR PRODUCTION |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2839433A1 true DE2839433A1 (en) | 1980-03-20 |
Family
ID=6049132
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19782839433 Withdrawn DE2839433A1 (en) | 1978-09-11 | 1978-09-11 | STANDARD AQUEOUS LIPID SOLUTION AND METHOD FOR THEIR PRODUCTION |
| DE7979103070T Expired DE2964278D1 (en) | 1978-09-11 | 1979-08-21 | Aqueous lipid standard solution |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE7979103070T Expired DE2964278D1 (en) | 1978-09-11 | 1979-08-21 | Aqueous lipid standard solution |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4289649A (en) |
| EP (1) | EP0009596B1 (en) |
| JP (1) | JPS5539095A (en) |
| DE (2) | DE2839433A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0067394A1 (en) * | 1981-06-10 | 1982-12-22 | Roche Diagnostics GmbH | Aqueous cholesterol standard solution and method of preparing the same |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3021457A1 (en) * | 1980-06-06 | 1982-01-07 | Boehringer Mannheim Gmbh, 6800 Mannheim | METHOD AND MEANS FOR SOLVING CHYLOMICRON IN AQUEOUS MEDIUM |
| FR2513766B1 (en) * | 1981-09-28 | 1985-09-13 | Biomerieux Sa | METHODS AND REAGENTS FOR SELECTIVE SEPARATION OF LOW DENSITY LDL LIPOPROTEINS AND FOR QUANTIFICATION OF THEIR COMPONENTS |
| DE3445010A1 (en) * | 1984-12-10 | 1986-06-19 | Boehringer Mannheim Gmbh | CONTROL OR OAK SERUM FOR LIPID DIAGNOSTICS |
| US5242832A (en) * | 1990-03-01 | 1993-09-07 | Toa Medical Electronics Co., Ltd. | Reagent for measurement of leukocytes and hemoglobin in blood |
| DE4018502A1 (en) * | 1990-06-09 | 1991-12-12 | Merck Patent Gmbh | METHOD AND MEANS FOR ELIMINATING TURBIDES IN BIOLOGICAL FLUIDS |
| US5385844A (en) * | 1993-04-22 | 1995-01-31 | Boehringer Mannheim Corporation | Polymer containing control reagents and polymers useful in control reagents |
| US5614414A (en) * | 1995-03-30 | 1997-03-25 | Streck Laboratories, Inc. | Liquid lipoprotein control |
| US5770451A (en) * | 1995-03-30 | 1998-06-23 | Streck Laboratories, Inc. | Liquid lipoprotein control |
| US5759445A (en) * | 1995-05-24 | 1998-06-02 | Matsushita Electric Industrial Co., Ltd. | Lipid-dispersed solution and process for producing the same |
| WO1998056719A2 (en) * | 1997-06-13 | 1998-12-17 | Medical Analysis Inc. | Control standards for clinical chemistry assays |
| ES2445148T3 (en) * | 2004-09-01 | 2014-02-28 | Maine Standards Company Llc | Novel stable lipid patterns |
| CN101762709B (en) * | 2008-12-19 | 2013-06-12 | 深圳迈瑞生物医疗电子股份有限公司 | Cholesterol water quality calibration solution and preparation method |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2185969A (en) * | 1935-05-24 | 1940-01-02 | Winthrop Chem Co Inc | Clear aqueous solutions of lipins and process of preparing the same |
| US2863734A (en) * | 1950-10-16 | 1958-12-09 | Schain Philip | Method and reagents for fat determination |
| US3062623A (en) * | 1958-10-13 | 1962-11-06 | Schain Philip | Reagent for the determination of fat in blood serum |
| DE1234417B (en) * | 1963-05-04 | 1967-02-16 | Merck Ag E | Test reagent for determining the fat content in organic substances, especially in blood serum |
| US3260648A (en) * | 1963-08-16 | 1966-07-12 | Warner Lambert Pharmaceutical | Diagnostic aid |
| US3754865A (en) * | 1972-01-05 | 1973-08-28 | Pierce Chemical Co | Colorimetric determination of calcium in biologic fluids |
| US3853465A (en) * | 1972-06-09 | 1974-12-10 | Technicon Instr | Turbidity reduction in serum and plasma samples using polyoxyethylated lauric acid compounds |
| DE2324386C2 (en) * | 1973-05-14 | 1975-07-03 | Boehringer Mannheim Gmbh, 6800 Mannheim | Aqueous cholestrin standard |
| US3859047A (en) * | 1973-05-29 | 1975-01-07 | Hoffmann La Roche | Standard for serum cholesterol determination |
| US4011045A (en) * | 1975-02-14 | 1977-03-08 | Bonderman Dean P | Turbidity reduction in triglyceride standards |
| AT354406B (en) * | 1975-08-12 | 1979-01-10 | Boehringer Mannheim Gmbh | METHOD AND REAGENT FOR DETERMINATION OF TRIGLYCERIDES |
| US4042330A (en) * | 1976-08-23 | 1977-08-16 | Deshmukh Arvind D | In a method for checking the accuracy of a test using an enzymatically hydrolyzable, serum-soluble cholesterol compound |
| US4040784A (en) * | 1976-08-23 | 1977-08-09 | Deshmukh Arvind D | Method for checking the accuracy of a test, using a serum-soluble cholesterol compound |
| US4110077A (en) * | 1976-10-08 | 1978-08-29 | Hoffmann-La Roche Inc. | Determination of β-lipoproteins in blood serum with polyanethole sulfonate |
| DE2653537C3 (en) * | 1976-11-25 | 1979-08-23 | Merck Patent Gmbh, 6100 Darmstadt | Method and means for the determination of hydroperoxides |
| DE2724757C2 (en) * | 1977-06-01 | 1979-08-23 | Boehringer Mannheim Gmbh, 6800 Mannheim | Means for removing cloudiness in serum and process for its preparation |
| US4189400A (en) * | 1977-08-17 | 1980-02-19 | Bonderman Dean P | Compound useful in cholesterol assay procedures |
-
1978
- 1978-09-11 DE DE19782839433 patent/DE2839433A1/en not_active Withdrawn
-
1979
- 1979-08-21 EP EP79103070A patent/EP0009596B1/en not_active Expired
- 1979-08-21 DE DE7979103070T patent/DE2964278D1/en not_active Expired
- 1979-09-10 JP JP11530279A patent/JPS5539095A/en active Pending
- 1979-09-11 US US06/074,457 patent/US4289649A/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0067394A1 (en) * | 1981-06-10 | 1982-12-22 | Roche Diagnostics GmbH | Aqueous cholesterol standard solution and method of preparing the same |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5539095A (en) | 1980-03-18 |
| EP0009596A1 (en) | 1980-04-16 |
| DE2964278D1 (en) | 1983-01-20 |
| US4289649A (en) | 1981-09-15 |
| EP0009596B1 (en) | 1982-12-15 |
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