CY1123180T1 - Συστημα ανιχνευσης που αξιοποιει αλυσιδωτη αντιδραση πολυμερασης - Google Patents

Συστημα ανιχνευσης που αξιοποιει αλυσιδωτη αντιδραση πολυμερασης

Info

Publication number
CY1123180T1
CY1123180T1 CY20201100629T CY201100629T CY1123180T1 CY 1123180 T1 CY1123180 T1 CY 1123180T1 CY 20201100629 T CY20201100629 T CY 20201100629T CY 201100629 T CY201100629 T CY 201100629T CY 1123180 T1 CY1123180 T1 CY 1123180T1
Authority
CY
Cyprus
Prior art keywords
polymerika
detection system
chain reaction
kits
methods
Prior art date
Application number
CY20201100629T
Other languages
English (en)
Inventor
Nisha JAIN
John Edmond HOLME
Original Assignee
Lgc Genomics Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lgc Genomics Limited filed Critical Lgc Genomics Limited
Publication of CY1123180T1 publication Critical patent/CY1123180T1/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/101—Interaction between at least two labels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

Η παρούσα εφεύρεση σχετίζεται με μεθόδους και με kit ανίχνευσης νουκλεϊκών οξέων σε ένα σύστημα προσδιορισμού.
CY20201100629T 2013-03-06 2020-07-08 Συστημα ανιχνευσης που αξιοποιει αλυσιδωτη αντιδραση πολυμερασης CY1123180T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB1304030.8A GB2515990B (en) 2013-03-06 2013-03-06 Polymerase chain reaction detection system
PCT/GB2014/050650 WO2014135872A1 (en) 2013-03-06 2014-03-05 Polymerase chain reaction detection system

Publications (1)

Publication Number Publication Date
CY1123180T1 true CY1123180T1 (el) 2021-10-29

Family

ID=48142528

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20201100629T CY1123180T1 (el) 2013-03-06 2020-07-08 Συστημα ανιχνευσης που αξιοποιει αλυσιδωτη αντιδραση πολυμερασης

Country Status (25)

Country Link
US (1) US10155975B2 (el)
EP (1) EP2964784B1 (el)
JP (1) JP6636335B2 (el)
CN (2) CN105051212A (el)
AU (1) AU2014224378B2 (el)
BR (1) BR112015021615B8 (el)
CA (1) CA2902920C (el)
CL (1) CL2015002475A1 (el)
CY (1) CY1123180T1 (el)
DK (1) DK2964784T3 (el)
ES (1) ES2795679T3 (el)
GB (1) GB2515990B (el)
HK (1) HK1216110A1 (el)
HR (1) HRP20201117T1 (el)
HU (1) HUE051443T2 (el)
LT (1) LT2964784T (el)
MY (1) MY182547A (el)
NZ (1) NZ711109A (el)
PL (1) PL2964784T3 (el)
PT (1) PT2964784T (el)
RS (1) RS60515B1 (el)
SG (1) SG11201506864XA (el)
SI (1) SI2964784T1 (el)
WO (1) WO2014135872A1 (el)
ZA (1) ZA201505950B (el)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201511156D0 (en) 2015-06-24 2015-08-05 Glaxosmithkline Australia Pty Ltd And University Of York Production of alkaloids
KR101899371B1 (ko) * 2017-07-25 2018-10-29 (주)엔바이오텍 핵산 복합체 페어, 핵산 복합체 페어를 포함하는 pcr용 키트, 및 핵산 복합체 페어를 이용한 타겟 검출 방법
GB201713251D0 (en) * 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
CN108715888A (zh) * 2018-06-07 2018-10-30 王卫东 一种基于fret的pcr均相检测***及其应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5750341A (en) * 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
CA2176193A1 (en) 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
EP1666609B1 (en) * 1997-02-28 2012-09-26 Quest Diagnostics Investments Incorporated Fluorescence energy transfer by competitive hybridization
WO1999049293A2 (en) * 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US20100105050A1 (en) * 2000-09-07 2010-04-29 Transnetyx, Inc. Real time detection of genetic sequences using a bipartite probe
WO2005030787A2 (en) * 2003-09-29 2005-04-07 Topigen Pharmaceutique Inc. Oligonucleotide compositions and methods for treating disease including inflammatory conditions
WO2006119326A2 (en) * 2005-05-02 2006-11-09 Stratagene California Oligonucleotide probe/primer compositions and methods for polynucleotide detection
GB0510979D0 (en) * 2005-05-28 2005-07-06 Kbiosciences Ltd Detection system for PCR assay
WO2009126678A2 (en) * 2008-04-08 2009-10-15 Cornell University Detection of target nucleic acid sequences using fluorescence resonance energy transfer
US9090650B2 (en) * 2010-07-28 2015-07-28 National University Corporation Hokkaido University Nucleic acid having adjuvanticity and use thereof
CN108342453A (zh) * 2011-05-09 2018-07-31 富鲁达公司 基于探针的核酸检测
JP6166342B2 (ja) * 2012-03-22 2017-07-19 エルジーシー・ジェノミクス・リミテッド ホスホロチオエート基を含むオリゴヌクレオチドを用いる、ポリメラーゼ連鎖反応による検出システム

Also Published As

Publication number Publication date
EP2964784A1 (en) 2016-01-13
US10155975B2 (en) 2018-12-18
GB201304030D0 (en) 2013-04-17
DK2964784T3 (da) 2020-06-15
CN116622816A (zh) 2023-08-22
AU2014224378A1 (en) 2015-09-03
EP2964784B1 (en) 2020-04-22
RS60515B1 (sr) 2020-08-31
GB2515990B (en) 2016-05-04
CA2902920C (en) 2019-07-30
HRP20201117T1 (hr) 2020-10-30
HUE051443T2 (hu) 2021-03-01
JP6636335B2 (ja) 2020-01-29
CN105051212A (zh) 2015-11-11
AU2014224378B2 (en) 2018-10-25
PT2964784T (pt) 2020-06-17
ZA201505950B (en) 2023-06-28
MY182547A (en) 2021-01-25
ES2795679T3 (es) 2020-11-24
BR112015021615B1 (pt) 2022-08-02
LT2964784T (lt) 2020-06-10
US20160002712A1 (en) 2016-01-07
WO2014135872A1 (en) 2014-09-12
BR112015021615B8 (pt) 2022-10-25
GB2515990A (en) 2015-01-14
SG11201506864XA (en) 2015-09-29
SI2964784T1 (sl) 2020-08-31
BR112015021615A2 (pt) 2018-03-06
NZ711109A (en) 2019-11-29
CL2015002475A1 (es) 2016-05-06
CA2902920A1 (en) 2014-09-12
PL2964784T3 (pl) 2020-10-19
JP2016513451A (ja) 2016-05-16
HK1216110A1 (zh) 2016-10-14

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