CY1119346T1 - Ισοθερμικη ενισχυση νουκλεικων οξεων - Google Patents
Ισοθερμικη ενισχυση νουκλεικων οξεωνInfo
- Publication number
- CY1119346T1 CY1119346T1 CY20171100921T CY171100921T CY1119346T1 CY 1119346 T1 CY1119346 T1 CY 1119346T1 CY 20171100921 T CY20171100921 T CY 20171100921T CY 171100921 T CY171100921 T CY 171100921T CY 1119346 T1 CY1119346 T1 CY 1119346T1
- Authority
- CY
- Cyprus
- Prior art keywords
- isothermal
- clone
- aucation
- invasion system
- oligonucleotide
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Μία ισοθερμική διαδικασία για την ενίσχυση ενός μορίου νουκλεϊνικού οξέος-στόχου που βασίζεται σε έναν ανοδικό εκκινητή, έναν καθοδικό εκκινητή, ένα σύστημα εισβολής κλώνου και ένα ολιγονουκλεοτίδιο. Οι ανοδικοί και οι καθοδικοί εκκινητές δεν είναι υποστρώματα για το σύστημα εισβολής κλώνου κατά τη διάρκεια της διαδικασίας της ενίσχυσης. Επίσης, δεν ενισχύουν το μόριο-στόχο ανεξαρτήτως από το σύστημα εισβολής κλώνου, όπου το ολιγονουκλεοτίδιο είναι ένα υπόστρωμα για το σύστημα εισβολής κλώνου.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0810650A GB0810650D0 (en) | 2008-06-11 | 2008-06-11 | Strand invasion based isothermal nucleic acid amplification |
GB0822533A GB0822533D0 (en) | 2008-12-11 | 2008-12-11 | Strand invasion based isothermal nucleic acid amplifications (2) |
PCT/GB2009/050662 WO2009150467A1 (en) | 2008-06-11 | 2009-06-11 | Isothermal nucleic acid amplification |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1119346T1 true CY1119346T1 (el) | 2018-03-07 |
Family
ID=40872336
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20171100921T CY1119346T1 (el) | 2008-06-11 | 2017-08-31 | Ισοθερμικη ενισχυση νουκλεικων οξεων |
CY20171100958T CY1119331T1 (el) | 2008-06-11 | 2017-09-11 | Ισοθερμικη ενισχυση νουκλεικων οξεων |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20171100958T CY1119331T1 (el) | 2008-06-11 | 2017-09-11 | Ισοθερμικη ενισχυση νουκλεικων οξεων |
Country Status (16)
Country | Link |
---|---|
US (3) | US9062344B2 (el) |
EP (3) | EP3249056B1 (el) |
JP (2) | JP5798917B2 (el) |
CN (2) | CN104862385B (el) |
CA (1) | CA2727212C (el) |
CY (2) | CY1119346T1 (el) |
DK (2) | DK2660336T3 (el) |
ES (1) | ES2638853T3 (el) |
HR (2) | HRP20171286T1 (el) |
HU (2) | HUE034561T2 (el) |
LT (2) | LT2304054T (el) |
PL (2) | PL2304054T3 (el) |
PT (2) | PT2304054T (el) |
SI (2) | SI2304054T1 (el) |
WO (1) | WO2009150467A1 (el) |
ZA (1) | ZA201008749B (el) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8293684B2 (en) * | 2006-11-29 | 2012-10-23 | Exiqon | Locked nucleic acid reagents for labelling nucleic acids |
CN104862385B (zh) | 2008-06-11 | 2018-11-23 | 基因排列技术有限公司 | 等温核酸扩增 |
US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
AU2011312218B2 (en) | 2010-10-04 | 2015-11-05 | Sequencing Health, Inc. | Systems and methods for automated reusable parallel biological reactions |
US9184099B2 (en) | 2010-10-04 | 2015-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Biosensor devices, systems and methods therefor |
US9399217B2 (en) | 2010-10-04 | 2016-07-26 | Genapsys, Inc. | Chamber free nanoreactor system |
CN103370425B (zh) | 2010-12-17 | 2019-03-19 | 生命技术公司 | 用于核酸扩增的方法、组合物、***、仪器和试剂盒 |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
US8585973B2 (en) | 2011-05-27 | 2013-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Nano-sensor array |
CN106591103B (zh) | 2011-12-01 | 2021-06-04 | 吉纳普赛斯股份有限公司 | 用于高效电子测序与检测的***和方法 |
CN102816756B (zh) * | 2012-09-07 | 2014-04-16 | 江苏奇天基因生物科技有限公司 | 等温核酸扩增反应试剂及等温核酸扩增方法 |
EP2971141B1 (en) | 2013-03-15 | 2018-11-28 | Genapsys, Inc. | Systems for biological analysis |
SG11201507261WA (en) | 2013-03-15 | 2015-10-29 | Theranos Inc | Nucleic acid amplification |
US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
CA2906824C (en) | 2013-03-15 | 2023-10-03 | Theranos, Inc. | Nucleic acid amplification |
KR20150132481A (ko) | 2013-03-15 | 2015-11-25 | 테라노스, 인코포레이티드 | 핵산 증폭 |
RU2673733C2 (ru) * | 2013-04-25 | 2018-11-29 | Орион Диагностика Ой | Способ амплификации днк на основе внедрения в цепь |
MX356055B (es) | 2013-09-06 | 2018-05-11 | Theranos Ip Co Llc | Sistemas y métodos para detectar enfermedades infecciosas. |
KR102324117B1 (ko) | 2013-11-22 | 2021-11-10 | 오리온 디아그노스티카 오와이 | 가닥 침입에 기초한 증폭에 의한 핵산 검출 |
EP3792921A1 (en) | 2013-12-11 | 2021-03-17 | Genapsys, Inc. | Systems and methods for biological analysis and computation |
EP3556864B1 (en) | 2014-04-18 | 2020-12-09 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
GB201410022D0 (en) * | 2014-06-05 | 2014-07-16 | Orion Diagnostica Oy | Method |
US11268117B2 (en) | 2016-06-10 | 2022-03-08 | Life Technologies Corporation | Methods and compositions for nucleic acid amplification |
EP3488017A4 (en) | 2016-07-20 | 2020-02-26 | Genapsys Inc. | SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING |
WO2018024562A1 (en) * | 2016-08-02 | 2018-02-08 | Roche Diagnostics Gmbh | Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids |
WO2018038232A1 (ja) * | 2016-08-24 | 2018-03-01 | 国立大学法人東北大学 | 標的核酸の増幅産物の生産方法及びその利用 |
GB201618035D0 (en) | 2016-10-25 | 2016-12-07 | Orion Diagnostica Oy | Method |
CN107058287B (zh) * | 2017-01-16 | 2020-06-16 | 浙江大学 | 一种恒温扩增体系中生成单链产物的方法 |
SG11202002516WA (en) | 2017-09-21 | 2020-04-29 | Genapsys Inc | Systems and methods for nucleic acid sequencing |
CN107893103A (zh) * | 2017-11-29 | 2018-04-10 | 默禾医疗科技(上海)有限公司 | 重组酶聚合酶扩增中重组酶和蛋白浓度比及活性定量法 |
EP3530755B1 (de) * | 2018-02-26 | 2020-08-26 | AGCT GmbH | Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung |
AU2019423311A1 (en) * | 2019-01-15 | 2021-07-15 | Tangen Biosciences Inc. | A method for suppressing non-specific amplification products in nucleic acid amplification technologies |
CN111926119B (zh) * | 2020-09-03 | 2023-04-21 | 上海市计量测试技术研究院 | 一种用于检测新型冠状病毒的核酸检测试剂盒及其使用方法 |
GB202018125D0 (en) | 2020-11-18 | 2020-12-30 | Aidian Oy | Method |
NL2033124B1 (en) | 2022-09-23 | 2024-03-29 | Rapidemic B V | Methods and device for multiple-label nucleic acid amplification and detection |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
AU3671099A (en) * | 1998-04-29 | 1999-11-16 | Trustees Of Boston University | Methods and compositions pertaining to pd-loops |
US6432642B1 (en) * | 1999-01-15 | 2002-08-13 | Pe Corporation (Ny) | Binary probe and clamp composition and methods for a target hybridization detection |
WO2000055353A1 (en) | 1999-03-17 | 2000-09-21 | The Board Of Trustees Of The Leland Stanford Junior University | In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system |
CA2382462A1 (en) * | 1999-08-20 | 2001-03-01 | Roche Diagnostics Gmbh | Enzymatic synthesis of deoxyribonucleosides |
JP3929775B2 (ja) * | 1999-09-13 | 2007-06-13 | ニューゲン テクノロジーズ, インコーポレイテッド | ポリヌクレオチド配列の線形等温増幅のための方法および組成物 |
US7399590B2 (en) * | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
WO2005118853A2 (en) * | 2004-06-01 | 2005-12-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
WO2006051988A1 (ja) | 2004-11-15 | 2006-05-18 | Riken | 核酸の増幅方法 |
EP1866434B1 (en) | 2005-02-19 | 2013-06-05 | Avacta Group plc | Isothermal nucleic acid amplification |
JP2006271372A (ja) | 2005-03-01 | 2006-10-12 | Yamasa Shoyu Co Ltd | 糖鎖の製造法 |
WO2007096702A2 (en) * | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
WO2008035205A2 (en) * | 2006-05-04 | 2008-03-27 | Asm Scientific, Inc. | Recombinase polymerase amplification |
CN104862385B (zh) | 2008-06-11 | 2018-11-23 | 基因排列技术有限公司 | 等温核酸扩增 |
-
2009
- 2009-06-11 CN CN201510103597.7A patent/CN104862385B/zh active Active
- 2009-06-11 HU HUE13178545A patent/HUE034561T2/en unknown
- 2009-06-11 US US12/997,382 patent/US9062344B2/en active Active
- 2009-06-11 CA CA2727212A patent/CA2727212C/en active Active
- 2009-06-11 DK DK13178545.3T patent/DK2660336T3/en active
- 2009-06-11 PT PT97620165T patent/PT2304054T/pt unknown
- 2009-06-11 WO PCT/GB2009/050662 patent/WO2009150467A1/en active Application Filing
- 2009-06-11 PL PL09762016T patent/PL2304054T3/pl unknown
- 2009-06-11 PL PL13178545T patent/PL2660336T3/pl unknown
- 2009-06-11 CN CN200980131223.9A patent/CN102119225B/zh active Active
- 2009-06-11 LT LTEP09762016.5T patent/LT2304054T/lt unknown
- 2009-06-11 SI SI200931718T patent/SI2304054T1/sl unknown
- 2009-06-11 DK DK09762016.5T patent/DK2304054T3/en active
- 2009-06-11 PT PT131785453T patent/PT2660336T/pt unknown
- 2009-06-11 ES ES09762016.5T patent/ES2638853T3/es active Active
- 2009-06-11 EP EP17179338.3A patent/EP3249056B1/en active Active
- 2009-06-11 EP EP13178545.3A patent/EP2660336B1/en active Active
- 2009-06-11 LT LTEP13178545.3T patent/LT2660336T/lt unknown
- 2009-06-11 SI SI200931719T patent/SI2660336T1/sl unknown
- 2009-06-11 HU HUE09762016A patent/HUE036005T2/hu unknown
- 2009-06-11 JP JP2011513055A patent/JP5798917B2/ja active Active
- 2009-06-11 EP EP09762016.5A patent/EP2304054B1/en active Active
-
2010
- 2010-12-06 ZA ZA2010/08749A patent/ZA201008749B/en unknown
-
2015
- 2015-05-12 US US14/709,793 patent/US9657340B2/en active Active
- 2015-08-21 JP JP2015164059A patent/JP6301881B2/ja active Active
-
2017
- 2017-04-12 US US15/485,689 patent/US10472659B2/en active Active
- 2017-08-28 HR HRP20171286TT patent/HRP20171286T1/hr unknown
- 2017-08-31 CY CY20171100921T patent/CY1119346T1/el unknown
- 2017-09-08 HR HRP20171356TT patent/HRP20171356T1/hr unknown
- 2017-09-11 CY CY20171100958T patent/CY1119331T1/el unknown
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CY1119346T1 (el) | Ισοθερμικη ενισχυση νουκλεικων οξεων | |
MX2019014264A (es) | Un metodo de amplificacion de transcriptoma unicelular. | |
WO2018135838A3 (ko) | Dna 단일가닥 절단에 의한 염기 교정 비표적 위치 확인 방법 | |
MX2020013836A (es) | Métodos, sistemas y diagnósticos de amplificación basados en el sistema efector crispr. | |
WO2008002920A3 (en) | Methods for generating target nucleic acid sequences | |
NZ701145A (en) | Compositions and methods for quantifying a nucleic acid sequence in a sample | |
WO2008143627A3 (en) | Targeted whole genome amplification method for identification of pathogens | |
WO2007123744A3 (en) | Systems and devices for sequence by synthesis analysis | |
CY1117177T1 (el) | Διεργασια για την παρασκευη [(3-υδροξυπυριδινο-2-καρβονυλ)αμινο]αλκανοϊκων οξεων, εστερων και αμιδιων | |
EP2379748A4 (en) | MULTIBASE RELEASE FOR LONG READINGS IN SEQUENCING BY SYNTHESIS PROTOCOLS | |
NZ629701A (en) | Target detection and signal amplification | |
ATE480644T1 (de) | Verfahren zur quantifizierung kleiner rna- moleküle | |
ATE329060T1 (de) | Mrna amplifizierung | |
WO2012083189A3 (en) | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification | |
WO2010107946A3 (en) | Use of thermostable endonucleases for generating reporter molecules | |
WO2008070675A3 (en) | Compositions and methods for the detection of small rna | |
DE602008006254D1 (de) | Doppel-oligonukleotid-nukleinsäurenachweisverfahren | |
ATE555219T1 (de) | Korrekturlese-primerverlängerung | |
BR112016029907A2 (pt) | enriquecimento geneticamente adaptado e reação em cadeia da polimerase para a rápida detecção de microrganismos". | |
MX2020013778A (es) | Composiciones, sistemas y metodos de amplificacion basados en crispr/cas y transposasa. | |
WO2014018958A3 (en) | Methods and devices for electromagnetic amplification of nucleic acids | |
WO2004068112A3 (en) | Hairpin primer amplification | |
MX2020010802A (es) | Moleculas inhibidoras de acidos nucleicos de caracter bicatenario modificadas con nucleotidos que aumentan la tm. | |
DE602005023002D1 (de) | Hybridisierungsverfahren | |
WO2008108843A3 (en) | Methods and kits for amplifying dna |