CY1119346T1 - Ισοθερμικη ενισχυση νουκλεικων οξεων - Google Patents

Ισοθερμικη ενισχυση νουκλεικων οξεων

Info

Publication number
CY1119346T1
CY1119346T1 CY20171100921T CY171100921T CY1119346T1 CY 1119346 T1 CY1119346 T1 CY 1119346T1 CY 20171100921 T CY20171100921 T CY 20171100921T CY 171100921 T CY171100921 T CY 171100921T CY 1119346 T1 CY1119346 T1 CY 1119346T1
Authority
CY
Cyprus
Prior art keywords
isothermal
clone
aucation
invasion system
oligonucleotide
Prior art date
Application number
CY20171100921T
Other languages
English (en)
Inventor
Mark Jay Hoser
Original Assignee
Orion Pharma (Uk) Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0810650A external-priority patent/GB0810650D0/en
Priority claimed from GB0822533A external-priority patent/GB0822533D0/en
Application filed by Orion Pharma (Uk) Limited filed Critical Orion Pharma (Uk) Limited
Publication of CY1119346T1 publication Critical patent/CY1119346T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Μία ισοθερμική διαδικασία για την ενίσχυση ενός μορίου νουκλεϊνικού οξέος-στόχου που βασίζεται σε έναν ανοδικό εκκινητή, έναν καθοδικό εκκινητή, ένα σύστημα εισβολής κλώνου και ένα ολιγονουκλεοτίδιο. Οι ανοδικοί και οι καθοδικοί εκκινητές δεν είναι υποστρώματα για το σύστημα εισβολής κλώνου κατά τη διάρκεια της διαδικασίας της ενίσχυσης. Επίσης, δεν ενισχύουν το μόριο-στόχο ανεξαρτήτως από το σύστημα εισβολής κλώνου, όπου το ολιγονουκλεοτίδιο είναι ένα υπόστρωμα για το σύστημα εισβολής κλώνου.
CY20171100921T 2008-06-11 2017-08-31 Ισοθερμικη ενισχυση νουκλεικων οξεων CY1119346T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0810650A GB0810650D0 (en) 2008-06-11 2008-06-11 Strand invasion based isothermal nucleic acid amplification
GB0822533A GB0822533D0 (en) 2008-12-11 2008-12-11 Strand invasion based isothermal nucleic acid amplifications (2)
PCT/GB2009/050662 WO2009150467A1 (en) 2008-06-11 2009-06-11 Isothermal nucleic acid amplification

Publications (1)

Publication Number Publication Date
CY1119346T1 true CY1119346T1 (el) 2018-03-07

Family

ID=40872336

Family Applications (2)

Application Number Title Priority Date Filing Date
CY20171100921T CY1119346T1 (el) 2008-06-11 2017-08-31 Ισοθερμικη ενισχυση νουκλεικων οξεων
CY20171100958T CY1119331T1 (el) 2008-06-11 2017-09-11 Ισοθερμικη ενισχυση νουκλεικων οξεων

Family Applications After (1)

Application Number Title Priority Date Filing Date
CY20171100958T CY1119331T1 (el) 2008-06-11 2017-09-11 Ισοθερμικη ενισχυση νουκλεικων οξεων

Country Status (16)

Country Link
US (3) US9062344B2 (el)
EP (3) EP3249056B1 (el)
JP (2) JP5798917B2 (el)
CN (2) CN104862385B (el)
CA (1) CA2727212C (el)
CY (2) CY1119346T1 (el)
DK (2) DK2660336T3 (el)
ES (1) ES2638853T3 (el)
HR (2) HRP20171286T1 (el)
HU (2) HUE034561T2 (el)
LT (2) LT2304054T (el)
PL (2) PL2304054T3 (el)
PT (2) PT2304054T (el)
SI (2) SI2304054T1 (el)
WO (1) WO2009150467A1 (el)
ZA (1) ZA201008749B (el)

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RU2673733C2 (ru) * 2013-04-25 2018-11-29 Орион Диагностика Ой Способ амплификации днк на основе внедрения в цепь
MX356055B (es) 2013-09-06 2018-05-11 Theranos Ip Co Llc Sistemas y métodos para detectar enfermedades infecciosas.
KR102324117B1 (ko) 2013-11-22 2021-11-10 오리온 디아그노스티카 오와이 가닥 침입에 기초한 증폭에 의한 핵산 검출
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GB201410022D0 (en) * 2014-06-05 2014-07-16 Orion Diagnostica Oy Method
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
EP3488017A4 (en) 2016-07-20 2020-02-26 Genapsys Inc. SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
WO2018024562A1 (en) * 2016-08-02 2018-02-08 Roche Diagnostics Gmbh Helper oligonucleotide for improving efficiency of amplification and detection/quantitation of nucleic acids
WO2018038232A1 (ja) * 2016-08-24 2018-03-01 国立大学法人東北大学 標的核酸の増幅産物の生産方法及びその利用
GB201618035D0 (en) 2016-10-25 2016-12-07 Orion Diagnostica Oy Method
CN107058287B (zh) * 2017-01-16 2020-06-16 浙江大学 一种恒温扩增体系中生成单链产物的方法
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing
CN107893103A (zh) * 2017-11-29 2018-04-10 默禾医疗科技（上海）有限公司 重组酶聚合酶扩增中重组酶和蛋白浓度比及活性定量法
EP3530755B1 (de) * 2018-02-26 2020-08-26 AGCT GmbH Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung
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Also Published As

Publication number Publication date
US9657340B2 (en) 2017-05-23
CN102119225B (zh) 2015-04-08
LT2304054T (lt) 2017-10-10
DK2304054T3 (en) 2017-09-18
PL2304054T3 (pl) 2017-12-29
JP2016005479A (ja) 2016-01-14
EP2304054B1 (en) 2017-08-16
EP2304054A1 (en) 2011-04-06
CN104862385A (zh) 2015-08-26
EP2660336A1 (en) 2013-11-06
WO2009150467A1 (en) 2009-12-17
US20110123991A1 (en) 2011-05-26
EP3249056A2 (en) 2017-11-29
PT2660336T (pt) 2017-09-13
US20170298401A1 (en) 2017-10-19
EP3249056A3 (en) 2018-02-14
JP5798917B2 (ja) 2015-10-21
EP2660336B1 (en) 2017-08-16
HUE034561T2 (en) 2018-02-28
CA2727212A1 (en) 2009-12-17
US20150307928A1 (en) 2015-10-29
CA2727212C (en) 2017-07-11
CN104862385B (zh) 2018-11-23
PL2660336T3 (pl) 2017-12-29
ZA201008749B (en) 2013-02-27
HUE036005T2 (hu) 2018-06-28
US9062344B2 (en) 2015-06-23
CN102119225A (zh) 2011-07-06
JP6301881B2 (ja) 2018-03-28
ES2638853T3 (es) 2017-10-24
DK2660336T3 (en) 2017-09-25
HRP20171286T1 (hr) 2017-11-17
SI2660336T1 (sl) 2017-11-30
LT2660336T (lt) 2017-10-10
CY1119331T1 (el) 2018-02-14
HRP20171356T1 (hr) 2017-12-15
PT2304054T (pt) 2017-09-05
US10472659B2 (en) 2019-11-12
SI2304054T1 (sl) 2017-11-30
JP2011522559A (ja) 2011-08-04
EP3249056B1 (en) 2019-04-10

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