CY1118864T1 - Μεθοδοι και συνθεσεις για μη επεμβατικη προγεννητικη διαγνωση εμβρυϊκων ανευπλοειδιων - Google Patents
Μεθοδοι και συνθεσεις για μη επεμβατικη προγεννητικη διαγνωση εμβρυϊκων ανευπλοειδιωνInfo
- Publication number
- CY1118864T1 CY1118864T1 CY20171100452T CY171100452T CY1118864T1 CY 1118864 T1 CY1118864 T1 CY 1118864T1 CY 20171100452 T CY20171100452 T CY 20171100452T CY 171100452 T CY171100452 T CY 171100452T CY 1118864 T1 CY1118864 T1 CY 1118864T1
- Authority
- CY
- Cyprus
- Prior art keywords
- dna
- hypomethylated
- fetal
- methods
- compositions
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Η εφεύρεση παρέχει μεθόδους και συνθέσεις για μη επεμβατική προγεννητική διάγνωση εμβρυϊκών ανευπλοειδιών. Έχει αναγνωριστεί μια μεγάλη ομάδα διαφορετικά μεθυλιωμένων περιοχών (DMRs). Ορισμένες από αυτές τις DMRs είναι υπομεθυλιωμένες στο DNA αίματος ενήλικων γυναικών και υπερμεθυλιωμένες στο εμβρυϊκό DNA, ενώ άλλες είναι υπερμεθυλιωμένες στο DNA αίματος ενήλικων γυναικών και υπομεθυλιωμένες στο εμβρυϊκό DNA. Επιπλέον, έχει δειχθεί ότι οι DMRs που είναι υπομεθυλιωμένες στο DNA αίματος ενήλικων γυναικών και υπερμεθυλιωμένες στο εμβρυϊκό DNA προβλέπουν με ακρίβεια μια εμβρυϊκή ανευπλοειδία στο εμβρυϊκό DNA που υπάρχει σε ένα δείγμα μητρικού αίματος κατά τη διάρκεια της κύησης. Στις μεθόδους της εφεύρεσης, το υπερμεθυλιωμένο DNA διαχωρίζεται από άποψη φυσιολογίας από το υπομεθυλιωμένο DNA, κατά προτίμηση με ανοσοκατακρήμνιση του μεθυλιωμένου DNA.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US29833910P | 2010-01-26 | 2010-01-26 | |
US40542110P | 2010-10-21 | 2010-10-21 | |
PCT/IB2011/000217 WO2011092592A2 (en) | 2010-01-26 | 2011-01-26 | Methods and compositions for noninvasive prenatal diagnosis of fetal aneuploidies |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1118864T1 true CY1118864T1 (el) | 2018-01-10 |
Family
ID=44278821
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20171100452T CY1118864T1 (el) | 2010-01-26 | 2017-04-21 | Μεθοδοι και συνθεσεις για μη επεμβατικη προγεννητικη διαγνωση εμβρυϊκων ανευπλοειδιων |
Country Status (17)
Country | Link |
---|---|
US (1) | US9249462B2 (el) |
EP (1) | EP2529032B1 (el) |
JP (1) | JP2013517789A (el) |
KR (1) | KR20120107512A (el) |
CN (1) | CN102892899A (el) |
AU (1) | AU2011210255B2 (el) |
BR (1) | BR112012018458A2 (el) |
CA (1) | CA2786174A1 (el) |
CY (1) | CY1118864T1 (el) |
DK (1) | DK2529032T3 (el) |
EA (1) | EA023565B1 (el) |
ES (1) | ES2623156T3 (el) |
NZ (1) | NZ601079A (el) |
PL (1) | PL2529032T3 (el) |
PT (1) | PT2529032T (el) |
SG (1) | SG182322A1 (el) |
WO (1) | WO2011092592A2 (el) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TWI335354B (en) | 2006-09-27 | 2011-01-01 | Univ Hong Kong Chinese | Methods for the detection of the degree of the methylation of a target dna and kits |
US8962247B2 (en) | 2008-09-16 | 2015-02-24 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses |
US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
ES2577017T3 (es) | 2009-12-22 | 2016-07-12 | Sequenom, Inc. | Procedimientos y kits para identificar la aneuploidia |
EP3757210B1 (en) | 2012-03-02 | 2022-08-24 | Sequenom, Inc. | Methods for enriching cancer nucleic acid from a biological sample |
US9920361B2 (en) | 2012-05-21 | 2018-03-20 | Sequenom, Inc. | Methods and compositions for analyzing nucleic acid |
CA2878979C (en) | 2012-07-13 | 2021-09-14 | Sequenom, Inc. | Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
DK3536807T3 (da) * | 2012-09-20 | 2024-02-05 | Univ Hong Kong Chinese | Ikkeinvasiv bestemmelse af foster- eller tumormethylom fra plasma |
US10706957B2 (en) | 2012-09-20 | 2020-07-07 | The Chinese University Of Hong Kong | Non-invasive determination of methylome of tumor from plasma |
US9732390B2 (en) | 2012-09-20 | 2017-08-15 | The Chinese University Of Hong Kong | Non-invasive determination of methylome of fetus or tumor from plasma |
SG11201502390QA (en) * | 2012-09-26 | 2015-05-28 | Agency Science Tech & Res | Biomarkers for down syndrome prenatal diagnosis |
EP2971100A1 (en) | 2013-03-13 | 2016-01-20 | Sequenom, Inc. | Primers for dna methylation analysis |
KR20140118682A (ko) | 2013-03-29 | 2014-10-08 | (주)셀트리온 | 2 이상의 인플루엔자 a 바이러스 중화 결합 분자를 포함하는 조성물 |
WO2015138774A1 (en) * | 2014-03-13 | 2015-09-17 | Sequenom, Inc. | Methods and processes for non-invasive assessment of genetic variations |
RU2583830C2 (ru) * | 2014-04-21 | 2016-05-10 | Закрытое акционерное общество "Геноаналитика" | Способ неинвазивной пренатальной диагностики анеуплоидий плода |
MA39951A (fr) | 2014-05-09 | 2017-03-15 | Lifecodexx Ag | Détection de l'adn provenant d'un type spécifique de cellule et méthodes associées |
EP2942401A1 (en) | 2014-05-09 | 2015-11-11 | Lifecodexx AG | Detection of DNA that originates from a specific cell-type |
EP2942400A1 (en) | 2014-05-09 | 2015-11-11 | Lifecodexx AG | Multiplex detection of DNA that originates from a specific cell-type |
US20180187267A1 (en) * | 2017-01-05 | 2018-07-05 | Michael J. Powell | Method for conducting early detection of colon cancer and/or of colon cancer precursor cells and for monitoring colon cancer recurrence |
US10174383B2 (en) * | 2014-08-13 | 2019-01-08 | Vanadis Diagnostics | Method of estimating the amount of a methylated locus in a sample |
WO2016176846A1 (zh) * | 2015-05-06 | 2016-11-10 | 安诺优达基因科技(北京)有限公司 | 检测染色体非整倍性的试剂盒、装置和方法 |
EP3666902B1 (en) | 2015-05-22 | 2024-07-03 | Medicover Public Co Ltd | Multiplexed parallel analysis of targeted genomic regions for non-invasive prenatal testing |
HUE050491T2 (hu) | 2015-11-10 | 2020-12-28 | Eurofins Lifecodexx Gmbh | Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között |
CN108475301A (zh) * | 2015-12-04 | 2018-08-31 | 绿十字基因组公司 | 用于确定包含核酸的混合物的样品中的拷贝数变异的方法 |
KR101817180B1 (ko) * | 2016-01-20 | 2018-01-10 | 이원다이애그노믹스(주) | 염색체 이상 판단 방법 |
DK3464637T3 (da) | 2016-05-30 | 2021-07-26 | Univ Hong Kong Chinese | Detektering af hæmatologiske forstyrrelser ved hjælp af cellefrit DNA i blod |
CN109112209B (zh) * | 2017-06-25 | 2022-06-24 | 国家卫生计生委科学技术研究所 | 用于无创产前检测胎儿非整倍体染色体的参考品 |
EP3765637A1 (en) | 2018-03-13 | 2021-01-20 | Grail, Inc. | Anomalous fragment detection and classification |
KR20200060969A (ko) | 2018-11-23 | 2020-06-02 | (주)셀트리온 | 인플루엔자 바이러스 질환을 치료하기 위한 투여 요법 |
EP4127223A1 (en) * | 2020-03-30 | 2023-02-08 | Vilnius University | Methods and compositions for noninvasive prenatal diagnosis through targeted covalent labeling of genomic sites |
KR102332540B1 (ko) * | 2020-07-02 | 2021-11-29 | 의료법인 성광의료재단 | 다운증후군 특이적인 후성학적 마커를 이용한 다운증후군 진단 방법 |
WO2023133572A2 (en) * | 2022-01-10 | 2023-07-13 | Washington State University | Dna methylation biomarkers for preterm birth |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6582908B2 (en) * | 1990-12-06 | 2003-06-24 | Affymetrix, Inc. | Oligonucleotides |
CA2540141C (en) | 2003-09-22 | 2012-09-04 | Trisogen Biotechnology Limited Partnership | Methods and kits useful for detecting an alteration in a locus copy number |
US20050282213A1 (en) * | 2003-09-22 | 2005-12-22 | Trisogen Biotechnology Limited Partnership | Methods and kits useful for detecting an alteration in a locus copy number |
GB0413688D0 (en) | 2004-06-18 | 2004-07-21 | Novartis Forschungsstiftung | Analysis of methylated nucleic acid |
CA2589487C (en) | 2004-11-29 | 2014-07-29 | Klinikum Der Universitat Regensburg | Means and methods for detecting methylated dna |
WO2007044780A2 (en) * | 2005-10-07 | 2007-04-19 | Emory University | Methods and systems for screening for and diagnosing dna methylation associated abnormalities and sex chromosome aneuploidies |
US7901884B2 (en) * | 2006-05-03 | 2011-03-08 | The Chinese University Of Hong Kong | Markers for prenatal diagnosis and monitoring |
CA2900927C (en) | 2007-07-23 | 2018-08-14 | The Chinese University Of Hong Kong | Diagnosing fetal chromosomal aneuploidy using genomic sequencing |
JP5322471B2 (ja) * | 2008-03-27 | 2013-10-23 | シスメックス株式会社 | メチル化dnaの解析方法及びプライマーセット |
US8476013B2 (en) * | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
-
2011
- 2011-01-26 JP JP2012550529A patent/JP2013517789A/ja active Pending
- 2011-01-26 PT PT117094656T patent/PT2529032T/pt unknown
- 2011-01-26 PL PL11709465T patent/PL2529032T3/pl unknown
- 2011-01-26 KR KR1020127020832A patent/KR20120107512A/ko not_active Application Discontinuation
- 2011-01-26 AU AU2011210255A patent/AU2011210255B2/en active Active
- 2011-01-26 SG SG2012049003A patent/SG182322A1/en unknown
- 2011-01-26 EP EP11709465.6A patent/EP2529032B1/en active Active
- 2011-01-26 CN CN2011800097571A patent/CN102892899A/zh active Pending
- 2011-01-26 EA EA201290716A patent/EA023565B1/ru not_active IP Right Cessation
- 2011-01-26 ES ES11709465.6T patent/ES2623156T3/es active Active
- 2011-01-26 WO PCT/IB2011/000217 patent/WO2011092592A2/en active Application Filing
- 2011-01-26 NZ NZ601079A patent/NZ601079A/en unknown
- 2011-01-26 US US13/520,708 patent/US9249462B2/en active Active
- 2011-01-26 BR BR112012018458A patent/BR112012018458A2/pt not_active IP Right Cessation
- 2011-01-26 DK DK11709465.6T patent/DK2529032T3/en active
- 2011-01-26 CA CA2786174A patent/CA2786174A1/en not_active Abandoned
-
2017
- 2017-04-21 CY CY20171100452T patent/CY1118864T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
PT2529032T (pt) | 2017-05-04 |
US9249462B2 (en) | 2016-02-02 |
EP2529032A2 (en) | 2012-12-05 |
AU2011210255A1 (en) | 2012-07-26 |
BR112012018458A2 (pt) | 2018-07-10 |
EA023565B1 (ru) | 2016-06-30 |
KR20120107512A (ko) | 2012-10-02 |
CA2786174A1 (en) | 2011-08-04 |
WO2011092592A3 (en) | 2011-11-03 |
SG182322A1 (en) | 2012-08-30 |
DK2529032T3 (en) | 2017-05-01 |
JP2013517789A (ja) | 2013-05-20 |
AU2011210255B2 (en) | 2014-11-20 |
ES2623156T3 (es) | 2017-07-10 |
CN102892899A (zh) | 2013-01-23 |
NZ601079A (en) | 2014-08-29 |
EP2529032B1 (en) | 2017-01-25 |
US20120282613A1 (en) | 2012-11-08 |
EA201290716A1 (ru) | 2013-06-28 |
PL2529032T3 (pl) | 2017-07-31 |
WO2011092592A2 (en) | 2011-08-04 |
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