CY1116480T1 - Βακτηριακο στελεχος ξενιστη που περιεχει ενα μεταλλαγμενο spr γονιδιο και μειωμενη δραση tsp - Google Patents

Βακτηριακο στελεχος ξενιστη που περιεχει ενα μεταλλαγμενο spr γονιδιο και μειωμενη δραση tsp

Info

Publication number
CY1116480T1
CY1116480T1 CY20151100525T CY151100525T CY1116480T1 CY 1116480 T1 CY1116480 T1 CY 1116480T1 CY 20151100525 T CY20151100525 T CY 20151100525T CY 151100525 T CY151100525 T CY 151100525T CY 1116480 T1 CY1116480 T1 CY 1116480T1
Authority
CY
Cyprus
Prior art keywords
bacterial host
host strain
strain containing
spr gene
mutant spr
Prior art date
Application number
CY20151100525T
Other languages
English (en)
Inventor
Mark Ellis
David Paul Humphreys
Original Assignee
Ucb Biopharma Sprl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb Biopharma Sprl filed Critical Ucb Biopharma Sprl
Publication of CY1116480T1 publication Critical patent/CY1116480T1/el

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Η παρούσα εφεύρεση παρέχει ένα ανασυνδυασμένο gram-αρνητικό βακτηριακό κύτταρο, το οποίο περιέχει ένα μεταλλαγμένο spr γονίδιο, το οποίο κωδικοποιεί μια spr πρωτεΐνη, το οποίο έχει μια μετάλλαξη σε ένα ή περισσότερα από τα αμινοξέα D133, Η145, Η157, Ν31, R62, 170, Q73, C94, S95, V98, Q99, R100, L108, Υ115, V135, L136, G140, R144 και G147 και όπου το κύτταρο έχει μειωμένη λειτουργία πρωτεΐνης Tsp συγκριτικά με την αντίστοιχη ενός άγριου τύπου κυττάρου.
CY20151100525T 2010-01-14 2015-06-18 Βακτηριακο στελεχος ξενιστη που περιεχει ενα μεταλλαγμενο spr γονιδιο και μειωμενη δραση tsp CY1116480T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1000587.4A GB201000587D0 (en) 2010-01-14 2010-01-14 Bacterial hoist strain
PCT/EP2011/050413 WO2011086136A1 (en) 2010-01-14 2011-01-13 Bacterial host strain comprising a mutant spr gene and having reduced tsp activity

Publications (1)

Publication Number Publication Date
CY1116480T1 true CY1116480T1 (el) 2018-03-07

Family

ID=42028357

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20151100525T CY1116480T1 (el) 2010-01-14 2015-06-18 Βακτηριακο στελεχος ξενιστη που περιεχει ενα μεταλλαγμενο spr γονιδιο και μειωμενη δραση tsp

Country Status (21)

Country Link
US (2) US8969039B2 (el)
EP (1) EP2524043B1 (el)
JP (1) JP5896569B2 (el)
KR (1) KR101737152B1 (el)
CN (2) CN102762732B (el)
AU (1) AU2011206584B2 (el)
BR (1) BR112012017425A2 (el)
CA (1) CA2786335C (el)
CY (1) CY1116480T1 (el)
DK (1) DK2524043T3 (el)
ES (1) ES2539931T3 (el)
GB (1) GB201000587D0 (el)
HK (1) HK1177760A1 (el)
HR (1) HRP20150659T1 (el)
PL (1) PL2524043T3 (el)
PT (1) PT2524043E (el)
RS (1) RS54084B1 (el)
SG (1) SG182301A1 (el)
SI (1) SI2524043T1 (el)
SM (1) SMT201500145B (el)
WO (1) WO2011086136A1 (el)

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GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
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GB201208370D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Antibodies
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BR112018015259A2 (pt) 2016-01-27 2018-12-18 Medimmune Llc métodos para preparação de anticorpos com um padrão de glicosilação definido
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CN114829578A (zh) * 2019-08-05 2022-07-29 瓦克化学股份公司 用于在发酵方法中释放重组蛋白的细菌菌株
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Also Published As

Publication number Publication date
US8969039B2 (en) 2015-03-03
SI2524043T1 (sl) 2015-07-31
CN105505845A (zh) 2016-04-20
KR101737152B1 (ko) 2017-05-17
HK1177760A1 (zh) 2013-08-30
KR20130030247A (ko) 2013-03-26
PT2524043E (pt) 2015-07-22
SG182301A1 (en) 2012-08-30
US9587227B2 (en) 2017-03-07
EP2524043B1 (en) 2015-03-18
RS54084B1 (en) 2015-10-30
PL2524043T3 (pl) 2016-08-31
AU2011206584A1 (en) 2012-07-26
GB201000587D0 (en) 2010-03-03
JP5896569B2 (ja) 2016-03-30
EP2524043A1 (en) 2012-11-21
US20150132828A1 (en) 2015-05-14
CA2786335C (en) 2017-10-10
BR112012017425A2 (pt) 2015-09-15
CN102762732B (zh) 2016-03-09
AU2011206584B2 (en) 2016-06-09
SMT201500145B (it) 2015-09-07
JP2013516964A (ja) 2013-05-16
DK2524043T3 (da) 2015-06-22
WO2011086136A1 (en) 2011-07-21
CA2786335A1 (en) 2011-07-21
ES2539931T3 (es) 2015-07-07
CN102762732A (zh) 2012-10-31
US20120295309A1 (en) 2012-11-22
HRP20150659T1 (hr) 2015-07-31

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