CY1110515T1 - Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος - Google Patents
Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεοςInfo
- Publication number
- CY1110515T1 CY1110515T1 CY20091101052T CY091101052T CY1110515T1 CY 1110515 T1 CY1110515 T1 CY 1110515T1 CY 20091101052 T CY20091101052 T CY 20091101052T CY 091101052 T CY091101052 T CY 091101052T CY 1110515 T1 CY1110515 T1 CY 1110515T1
- Authority
- CY
- Cyprus
- Prior art keywords
- compound
- protein
- library
- fusion
- union
- Prior art date
Links
- 230000004927 fusion Effects 0.000 title abstract 4
- 230000003993 interaction Effects 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title 1
- 150000001875 compounds Chemical class 0.000 abstract 6
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Στο παρόν ανακοινώνεται μέθοδος εντοπισμού αλληλεπίδρασης ένωσης-πρωτεΐνης που περιλαμβάνει: (α) παροχή βιβλιοθήκης ενώσεων στην οποία κάθε μέλος της βιβλιοθήκης ενώσεων είναι ακινητοποιημένο σε στερεό μέσο υποστήριξης, (β) επαφή κάθε μέλους της ακινητοποιημένης βιβλιοθήκης ενώσεων σε μονό θάλαμο αντίδρασης, με κάθε μέλος της βιβλιοθήκης συντήξεων πρωτεΐνης-νουκλεϊκού οξέος, υπό συνθήκες που επιτρέπουν το σχηματισμό συμπλεγμάτων ένωσης-σύντηξης, (γ) απομόνωση των ακινητοποιημένων συμπλεγμάτων ένωσης-σύντηξης, και (δ) εντοπισμό συμπλέγματος ένωσης-σύντηξης ως ένδειξη ότι η πρωτεΐνη της σύντηξης αλληλεπιδρά με την ένωση. Σε προτιμώμενες εφαρμογές, η πρωτεΐνη προσδιορίζεται με ανάγνωση του τμήματος νουκλεϊκού οξέος της σύντηξης, και η ένωση προσδιορίζεται με ανάγνωση ανιχνεύσιμης ετικέτας που είναι δεσμευμένη είτε στην ένωση είτε στο στερεό μέσο υποστήριξης.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9682098P | 1998-08-17 | 1998-08-17 | |
EP99948042A EP1105360B1 (en) | 1998-08-17 | 1999-08-16 | Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules |
Publications (1)
Publication Number | Publication Date |
---|---|
CY1110515T1 true CY1110515T1 (el) | 2015-04-29 |
Family
ID=22259237
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CY20091101052T CY1110515T1 (el) | 1998-08-17 | 2009-10-13 | Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος |
Country Status (12)
Country | Link |
---|---|
US (1) | US6602685B1 (el) |
EP (1) | EP1105360B1 (el) |
JP (1) | JP4278872B2 (el) |
AT (1) | ATE437844T1 (el) |
AU (1) | AU757955B2 (el) |
CA (1) | CA2337490C (el) |
CY (1) | CY1110515T1 (el) |
DE (1) | DE69941193D1 (el) |
DK (1) | DK1105360T3 (el) |
ES (1) | ES2329205T3 (el) |
PT (1) | PT1105360E (el) |
WO (1) | WO2000009464A1 (el) |
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-
1999
- 1999-08-16 DE DE69941193T patent/DE69941193D1/de not_active Expired - Lifetime
- 1999-08-16 US US09/374,964 patent/US6602685B1/en not_active Expired - Lifetime
- 1999-08-16 ES ES99948042T patent/ES2329205T3/es not_active Expired - Lifetime
- 1999-08-16 DK DK99948042T patent/DK1105360T3/da active
- 1999-08-16 WO PCT/US1999/018600 patent/WO2000009464A1/en active IP Right Grant
- 1999-08-16 PT PT99948042T patent/PT1105360E/pt unknown
- 1999-08-16 AT AT99948042T patent/ATE437844T1/de active
- 1999-08-16 JP JP2000564919A patent/JP4278872B2/ja not_active Expired - Lifetime
- 1999-08-16 CA CA2337490A patent/CA2337490C/en not_active Expired - Lifetime
- 1999-08-16 AU AU61296/99A patent/AU757955B2/en not_active Expired
- 1999-08-16 EP EP99948042A patent/EP1105360B1/en not_active Expired - Lifetime
-
2009
- 2009-10-13 CY CY20091101052T patent/CY1110515T1/el unknown
Also Published As
Publication number | Publication date |
---|---|
WO2000009464A1 (en) | 2000-02-24 |
WO2000009464A9 (en) | 2000-09-28 |
EP1105360B1 (en) | 2009-07-29 |
CA2337490A1 (en) | 2000-02-24 |
ATE437844T1 (de) | 2009-08-15 |
DK1105360T3 (da) | 2009-11-02 |
US6602685B1 (en) | 2003-08-05 |
AU6129699A (en) | 2000-03-06 |
JP4278872B2 (ja) | 2009-06-17 |
EP1105360A4 (en) | 2005-06-22 |
EP1105360A1 (en) | 2001-06-13 |
JP2002522057A (ja) | 2002-07-23 |
PT1105360E (pt) | 2009-09-30 |
DE69941193D1 (de) | 2009-09-10 |
CA2337490C (en) | 2012-01-03 |
ES2329205T3 (es) | 2009-11-23 |
AU757955B2 (en) | 2003-03-13 |
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