CY1110515T1 - Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος - Google Patents

Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος

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Publication number
CY1110515T1
CY1110515T1 CY20091101052T CY091101052T CY1110515T1 CY 1110515 T1 CY1110515 T1 CY 1110515T1 CY 20091101052 T CY20091101052 T CY 20091101052T CY 091101052 T CY091101052 T CY 091101052T CY 1110515 T1 CY1110515 T1 CY 1110515T1
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CY
Cyprus
Prior art keywords
compound
protein
library
fusion
union
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Application number
CY20091101052T
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English (en)
Inventor
Peter Lohse
Original Assignee
Bristol-Myers Squibb Company
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Filing date
Publication date
Application filed by Bristol-Myers Squibb Company filed Critical Bristol-Myers Squibb Company
Publication of CY1110515T1 publication Critical patent/CY1110515T1/el

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Στο παρόν ανακοινώνεται μέθοδος εντοπισμού αλληλεπίδρασης ένωσης-πρωτεΐνης που περιλαμβάνει: (α) παροχή βιβλιοθήκης ενώσεων στην οποία κάθε μέλος της βιβλιοθήκης ενώσεων είναι ακινητοποιημένο σε στερεό μέσο υποστήριξης, (β) επαφή κάθε μέλους της ακινητοποιημένης βιβλιοθήκης ενώσεων σε μονό θάλαμο αντίδρασης, με κάθε μέλος της βιβλιοθήκης συντήξεων πρωτεΐνης-νουκλεϊκού οξέος, υπό συνθήκες που επιτρέπουν το σχηματισμό συμπλεγμάτων ένωσης-σύντηξης, (γ) απομόνωση των ακινητοποιημένων συμπλεγμάτων ένωσης-σύντηξης, και (δ) εντοπισμό συμπλέγματος ένωσης-σύντηξης ως ένδειξη ότι η πρωτεΐνη της σύντηξης αλληλεπιδρά με την ένωση. Σε προτιμώμενες εφαρμογές, η πρωτεΐνη προσδιορίζεται με ανάγνωση του τμήματος νουκλεϊκού οξέος της σύντηξης, και η ένωση προσδιορίζεται με ανάγνωση ανιχνεύσιμης ετικέτας που είναι δεσμευμένη είτε στην ένωση είτε στο στερεό μέσο υποστήριξης.
CY20091101052T 1998-08-17 2009-10-13 Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος CY1110515T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US9682098P 1998-08-17 1998-08-17
EP99948042A EP1105360B1 (en) 1998-08-17 1999-08-16 Identification of compound-protein interactions using libraries of protein-nucleic acid fusion molecules

Publications (1)

Publication Number Publication Date
CY1110515T1 true CY1110515T1 (el) 2015-04-29

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Family Applications (1)

Application Number Title Priority Date Filing Date
CY20091101052T CY1110515T1 (el) 1998-08-17 2009-10-13 Προσδιορισμος αλληλεπιδρασεων ενωσης-πρωτεϊνης με χρηση βιβλιοθηκων μοριων συντηξης πρωτεϊνης-νουκλεϊκου οξεος

Country Status (12)

Country Link
US (1) US6602685B1 (el)
EP (1) EP1105360B1 (el)
JP (1) JP4278872B2 (el)
AT (1) ATE437844T1 (el)
AU (1) AU757955B2 (el)
CA (1) CA2337490C (el)
CY (1) CY1110515T1 (el)
DE (1) DE69941193D1 (el)
DK (1) DK1105360T3 (el)
ES (1) ES2329205T3 (el)
PT (1) PT1105360E (el)
WO (1) WO2000009464A1 (el)

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Also Published As

Publication number Publication date
WO2000009464A1 (en) 2000-02-24
WO2000009464A9 (en) 2000-09-28
EP1105360B1 (en) 2009-07-29
CA2337490A1 (en) 2000-02-24
ATE437844T1 (de) 2009-08-15
DK1105360T3 (da) 2009-11-02
US6602685B1 (en) 2003-08-05
AU6129699A (en) 2000-03-06
JP4278872B2 (ja) 2009-06-17
EP1105360A4 (en) 2005-06-22
EP1105360A1 (en) 2001-06-13
JP2002522057A (ja) 2002-07-23
PT1105360E (pt) 2009-09-30
DE69941193D1 (de) 2009-09-10
CA2337490C (en) 2012-01-03
ES2329205T3 (es) 2009-11-23
AU757955B2 (en) 2003-03-13

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