CY1110012T1 - Συστημα ανιχνευσης για δοκιμασια pcr - Google Patents

Συστημα ανιχνευσης για δοκιμασια pcr

Info

Publication number
CY1110012T1
CY1110012T1 CY20101100361T CY101100361T CY1110012T1 CY 1110012 T1 CY1110012 T1 CY 1110012T1 CY 20101100361 T CY20101100361 T CY 20101100361T CY 101100361 T CY101100361 T CY 101100361T CY 1110012 T1 CY1110012 T1 CY 1110012T1
Authority
CY
Cyprus
Prior art keywords
detection system
test detection
pcr test
pcr process
sequences
Prior art date
Application number
CY20101100361T
Other languages
English (en)
Inventor
Philip Steven Robinson
Original Assignee
Kbiosciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34834826&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CY1110012(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Kbiosciences Ltd filed Critical Kbiosciences Ltd
Publication of CY1110012T1 publication Critical patent/CY1110012T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Geophysics And Detection Of Objects (AREA)
  • Monitoring And Testing Of Transmission In General (AREA)
  • Maintenance And Management Of Digital Transmission (AREA)
  • Time-Division Multiplex Systems (AREA)

Abstract

Η παρούσα εφεύρεση παρέχει ένα σύστημα ανίχνευσης για μια διεργασία PCR χρησιμοποιώντας FRET η οποία αποτελείται από τουλάχιστον δυο ολιγονουκλεοτιδικές αλληλουχίες με μια σήμανση, διαφορετικού Tm, οι οποίες υβριδίζονται η μια προς την άλλη σε ελεύθερο διάλυμα για να σχηματίσουν ένα φθορίζον κεχρωσμένο ζεύγος, το οποίο κατά την εισαγωγή μιας συμπληρωματικής αλληλουχίας σε μια ή αμφότερες τις αλληλουχίες δημιουργεί ένα μετρήσιμο σήμα, με μια από τις αλληλουχίες να είναι μιας Tm η οποία είναι κάτω από την Ta της διεργασίας PCR, και την άλλη να μην είναι κάτω από την Ta της διεργασίας PCR.
CY20101100361T 2005-05-28 2010-04-22 Συστημα ανιχνευσης για δοκιμασια pcr CY1110012T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0510979.8A GB0510979D0 (en) 2005-05-28 2005-05-28 Detection system for PCR assay
EP06252796A EP1726664B1 (en) 2005-05-28 2006-05-30 Detection system for PCR assay

Publications (1)

Publication Number Publication Date
CY1110012T1 true CY1110012T1 (el) 2015-01-14

Family

ID=34834826

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20101100361T CY1110012T1 (el) 2005-05-28 2010-04-22 Συστημα ανιχνευσης για δοκιμασια pcr

Country Status (11)

Country Link
US (1) US7615620B2 (el)
EP (1) EP1726664B1 (el)
AT (1) ATE456670T1 (el)
CY (1) CY1110012T1 (el)
DE (1) DE602006011976D1 (el)
DK (1) DK1726664T3 (el)
ES (1) ES2340058T3 (el)
GB (2) GB0510979D0 (el)
PL (1) PL1726664T3 (el)
PT (1) PT1726664E (el)
SI (1) SI1726664T1 (el)

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DE602008006254D1 (de) * 2007-09-28 2011-05-26 3M Innovative Properties Co Doppel-oligonukleotid-nukleinsäurenachweisverfahren
US20110136105A1 (en) * 2009-12-05 2011-06-09 Evogen, Inc. Methods of quantifying nucleic acids
US9644231B2 (en) 2011-05-09 2017-05-09 Fluidigm Corporation Nucleic acid detection using probes
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DE102011078342A1 (de) * 2011-06-29 2013-01-03 Aj Innuscreen Gmbh Rehybridisierendes Sondensystem zur qualitativen und quantitativen Messung von spezifischen Nukleinsäuren in Echtzeit
RS55868B1 (sr) 2012-03-22 2017-08-31 Lgc Genomics Ltd Sistem za detekciju lančane reakcije polimeraze pomoću oligonukleotida koji sadrže fosforotioatnu grupu
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
CA2882079A1 (en) 2012-05-30 2015-01-29 Dow Agrosciences Llc Floury 2 gene-specific assay in maize for floury (fl2) trait introgression
GB2515990B (en) 2013-03-06 2016-05-04 Lgc Genomics Ltd Polymerase chain reaction detection system
EP2971095B1 (en) * 2013-03-12 2019-11-20 Life Technologies Corporation Universal reporter-based genotyping methods, reaction mixture and kit
GB2512631A (en) * 2013-04-03 2014-10-08 Rupert Maxwell Gaut Quantitative detection of specific nucleic acid sequences
EP2975136A1 (en) 2014-07-14 2016-01-20 Parlanca Limited A method for detecting PCR amplification in a sample
IES20150206A2 (en) 2014-07-14 2016-03-09 Identigen Ltd A method for detecting PCR amplification in a sample
WO2017120309A1 (en) * 2016-01-05 2017-07-13 The Texas A&M University System Comparative locus amplification for determining copy number
MX2018012884A (es) 2016-04-20 2019-03-28 Bayer Cropscience Nv Evento elite ee-gh7 y metodos y kits para la identificacion de dicho evento en muestras biologicas.
CN105861678B (zh) * 2016-04-29 2019-12-13 广州市康立明生物科技有限责任公司 一种用于扩增低浓度突变靶序列的引物和探针的设计方法
CN110312804A (zh) 2016-12-22 2019-10-08 巴斯夫农业种子解决方案美国有限责任公司 原种事件ee-gm4及在生物样品中用于鉴定该事件的方法和试剂盒
EP3559242A1 (en) 2016-12-22 2019-10-30 Basf Agricultural Solutions Seed Us Llc Elite event ee-gm5 and methods and kits for identifying such event in biological samples
GB201713251D0 (en) 2017-08-18 2017-10-04 Lgc Genomics Ltd Methods and kits for detection of nucleic acid molecules
WO2019187467A1 (ja) 2018-03-28 2019-10-03 ソニー株式会社 標的物質検出方法、標的物質検出用キット及び標的物質検出装置
GB2587178B (en) 2019-01-09 2021-10-06 3Cr Bioscience Ltd Method
GB2587177A (en) 2019-01-09 2021-03-24 3Cr Bioscience Ltd Polymerase
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JP2023544432A (ja) 2020-10-12 2023-10-23 ヌンヘムス ビー．ブイ． 単為結果性スイカ植物体
MX2023005394A (es) 2020-11-09 2023-05-19 Nunhems Bv Plantas de sandia partenocarpicas.
WO2023275048A1 (en) 2021-06-30 2023-01-05 Nunhems B.V. Methods for selecting watermelon plants and plant parts comprising a modified dwarf14 gene
WO2023020938A1 (en) 2021-08-16 2023-02-23 Nunhems B.V. Lettuce plant having delayed bolting
CN114540345B (zh) * 2021-11-03 2024-04-09 武汉蓝沙医学检验实验室有限公司 一种发夹结构的标签荧光探针和荧光检测方法
GB202119080D0 (en) 2021-12-29 2022-02-09 3Cr Bioscience Ltd Method
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Also Published As

Publication number Publication date
GB0510979D0 (en) 2005-07-06
EP1726664B1 (en) 2010-01-27
PT1726664E (pt) 2010-04-14
ES2340058T3 (es) 2010-05-28
GB2426520A (en) 2006-11-29
PL1726664T3 (pl) 2010-07-30
DE602006011976D1 (de) 2010-03-18
ATE456670T1 (de) 2010-02-15
EP1726664A1 (en) 2006-11-29
GB0610653D0 (en) 2006-07-05
SI1726664T1 (sl) 2010-06-30
DK1726664T3 (da) 2010-05-17
US20070117108A1 (en) 2007-05-24
US7615620B2 (en) 2009-11-10

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