CY1106724T1 - Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση ή και προσδιορισμο ειδικων αλληλουχιων νουκλεϊκων οξεων - Google Patents
Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση ή και προσδιορισμο ειδικων αλληλουχιων νουκλεϊκων οξεωνInfo
- Publication number
- CY1106724T1 CY1106724T1 CY20071100930T CY071100930T CY1106724T1 CY 1106724 T1 CY1106724 T1 CY 1106724T1 CY 20071100930 T CY20071100930 T CY 20071100930T CY 071100930 T CY071100930 T CY 071100930T CY 1106724 T1 CY1106724 T1 CY 1106724T1
- Authority
- CY
- Cyprus
- Prior art keywords
- map
- determination
- nucleic acid
- acid sequences
- specific
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 150000007523 nucleic acids Chemical group 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 3
- 238000003556 assay Methods 0.000 abstract 3
- 108010090804 Streptavidin Proteins 0.000 abstract 2
- 108020000946 Bacterial DNA Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108020005202 Viral DNA Proteins 0.000 abstract 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 239000012491 analyte Substances 0.000 abstract 1
- 239000011324 bead Substances 0.000 abstract 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 abstract 1
- 239000010931 gold Substances 0.000 abstract 1
- 229910052737 gold Inorganic materials 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 230000001575 pathological effect Effects 0.000 abstract 1
- 230000003612 virological effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Εμβαπτιζόμενος χάρτης αποξηραμένων αντιδραστηρίων, μέθοδος παραγωγής του, και μέθοδος για την ανίχνευση ή και προσδιορισμό ειδικών αλληλουχιών νουκλεϊκών οξέων με την ανάπτυξη πρωτοκόλλων δοκιμασιών υβριδισμού σε αυτόν. Στο χάρτη αποξηραίνονται σφαιρίδια χρυσού συζευγμένα με ολιγονουκλεοτίδια, και στην περιοχή της μεμβράνης ακινητοποιείται η πρωτεΐνη στρεπταβιδίνη και ένα ολιγονουκλεοτίδιο. Ανιχνεύεται η παρουσία μιας ειδικής αλληλουχίας DΝΑ όταν λαμβάνεται έγχρωμο σήμα ενδεικτικό της παρουσίας ή απουσίας του αναλυτή στην ζώνη ακινητοποίησης της στρεπταβιδίνης, και επιβεβαιώνεται η ολοκλήρωση της δοκιμασίας από την ανάπτυξη έγχρωμου σήματος ενδεικτικού της ολοκλήρωσης της δοκιμασίας στη ζώνη ακινητοποίησης του ολιγονουκλεοτιδίου. Ο εμβαπτιζόμενος χάρτης χρησιμεύει στην ταχεία ανίχνευση ή και προσδιορισμό της ειδικής αλληλουχίας νουκλεϊκών οξέων που δηλώνουν π.χ. την παρουσία ιϊκού ή βακτηριακού DΝΑ ή DΝΑ μεταλλαγμένου στο οποίο οφείλεται παθολογικός φαινότυπος ασθενή, στο υπό εξέταση δείγμα μετά την εκλεκτική ενίσχυση της ειδικής αλληλουχίας στο δείγμα μέσω ΡCR.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GR20010100478 | 2001-10-16 | ||
| PCT/GR2002/000052 WO2003033735A2 (en) | 2001-10-16 | 2002-10-03 | Test strip assay system and method for the detection of specific nucleic acid sequences |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CY1106724T1 true CY1106724T1 (el) | 2012-05-23 |
Family
ID=37616771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CY20071100930T CY1106724T1 (el) | 2001-10-16 | 2007-07-13 | Εμβαπτιζομενος χαρτης αποξηραμενων αντιδραστηριων και μεθοδος για την ανιχνευση ή και προσδιορισμο ειδικων αλληλουχιων νουκλεϊκων οξεων |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US20050202427A1 (el) |
| EP (1) | EP1436420B1 (el) |
| AT (1) | ATE360708T1 (el) |
| AU (1) | AU2002329496B2 (el) |
| BR (1) | BR0206145A (el) |
| CA (1) | CA2468802A1 (el) |
| CY (1) | CY1106724T1 (el) |
| DE (1) | DE60219813T2 (el) |
| DK (1) | DK1436420T3 (el) |
| ES (1) | ES2286276T3 (el) |
| GR (1) | GR1003966B (el) |
| NO (1) | NO20032697L (el) |
| PT (1) | PT1436420E (el) |
| WO (1) | WO2003033735A2 (el) |
| YU (1) | YU48903A (el) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GR1004393B (el) | 2002-11-07 | 2003-11-28 | ΑΥΤΟΜΑΤΟΙ ΑΝΑΛΥΤΕΣ ΚΑΙ ΔΙΑΓΝΩΣΤΙΚΑ ΑΝΤΙΔΡΑΣΤΗΡΙΑ MEDICON HELLAS ΑΝΩΝΥΜΗ ΕΤΑΙΡΕΙΑ δ.τ. MEDICON HELLAS S.A. | Μεθοδος ανιχνευσης ειδικων αλληλουχιων νουκλεικων οξεων σε ταινια ξηρων αντιδραστηριων |
| ES2363501T3 (es) | 2003-05-07 | 2011-08-05 | Coris Bioconcept Sprl | Dispositivo oligocromático de un paso y procedimiento de uso. |
| EP1657550A1 (en) * | 2004-11-10 | 2006-05-17 | Coris Bioconcept SPRL | Double-sided device for multiplex dipstick immunodiagnostic |
| DE602005026578D1 (de) * | 2004-12-29 | 2011-04-07 | Suzhou Yangze Delta Academy Of Bio X Science Ltd | Optimales verfahren zur amplifikation nach polymerasekettenreaktion |
| GB0503986D0 (en) * | 2005-02-26 | 2005-04-06 | Secr Defence | Reaction apparatus |
| US8980561B1 (en) * | 2006-08-22 | 2015-03-17 | Los Alamos National Security, Llc. | Nucleic acid detection system and method for detecting influenza |
| EP1933145B1 (en) * | 2006-12-11 | 2011-09-28 | AraGen Biotechnology Co. Ltd. | Rapid immunochromatographic detection by amplification of the colloidal gold signal |
| WO2009005552A2 (en) * | 2007-03-29 | 2009-01-08 | Epitype Corporation | Methods and compositions for multivalent binding and methods for manufacture of rapid diagnostic tests |
| GB0806041D0 (en) * | 2008-04-03 | 2008-05-14 | Genomica S A | Method for detection of herpesvirus in test sample |
| CN102128832A (zh) * | 2010-12-27 | 2011-07-20 | 江南大学 | 一种西瓜果斑病毒金标核酸试纸条及其制备和应用 |
| CN102183633B (zh) * | 2011-02-24 | 2013-05-15 | 南京基蛋生物科技有限公司 | 一种胶体金标记方法 |
| US9651549B2 (en) * | 2012-07-13 | 2017-05-16 | Genisphere, Llc | Lateral flow assays using DNA dendrimers |
| FR3012982B1 (fr) * | 2013-11-08 | 2015-12-25 | Espci Innov | Procede de stockage et de concentration d'un compose volatil |
| EP3218053B1 (en) | 2014-11-13 | 2019-05-29 | The Alfred E. Mann Foundation for Scientific Research | Percutaneous lead interface |
| US9464969B2 (en) | 2014-11-20 | 2016-10-11 | Monolythix, Inc. | Monoliths |
| CA2978610A1 (en) | 2015-03-03 | 2016-09-09 | University Of Miami | Kits and methods for pathogen detection |
| US20170051274A1 (en) | 2015-08-18 | 2017-02-23 | Monolythix, Inc. | Sample concentration devices |
| CN108977501A (zh) * | 2018-08-13 | 2018-12-11 | 吉林农业科技学院 | 人参黑斑菌-单管巢式pcr-核酸传感器的制备及其检测方法 |
| ES2932995A1 (es) * | 2021-07-22 | 2023-01-30 | Ocupharm Diagnostics Sl | Sistema de detección de parásitos oculares |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| WO1990001564A1 (en) * | 1988-08-09 | 1990-02-22 | Microprobe Corporation | Methods for multiple target analyses through nucleic acid hybridization |
| WO1992007096A1 (en) * | 1990-10-19 | 1992-04-30 | Microprobe Corporation | Mehods and pharmaceutical kits useful for detecting microorganisms associated with vaginal infections |
| US6582921B2 (en) * | 1996-07-29 | 2003-06-24 | Nanosphere, Inc. | Nanoparticles having oligonucleotides attached thereto and uses thereof |
| US20010026944A1 (en) * | 1999-04-21 | 2001-10-04 | Roy Chung | Immunoassay system |
| DE60045739D1 (de) * | 1999-06-25 | 2011-04-28 | Nanosphere Inc | Nanopartikel mit gebundenen oligonukleotiden und deren verwendungen |
| US7101683B2 (en) * | 2001-06-26 | 2006-09-05 | Abbott Laboratories | Methods for the simultaneous detection of HCV antigens and HCV antibodies |
-
2001
- 2001-10-16 GR GR20010100478A patent/GR1003966B/el unknown
-
2002
- 2002-10-03 ES ES02765125T patent/ES2286276T3/es not_active Expired - Lifetime
- 2002-10-03 WO PCT/GR2002/000052 patent/WO2003033735A2/en active IP Right Grant
- 2002-10-03 PT PT02765125T patent/PT1436420E/pt unknown
- 2002-10-03 YU YU48903A patent/YU48903A/sh unknown
- 2002-10-03 CA CA002468802A patent/CA2468802A1/en not_active Abandoned
- 2002-10-03 EP EP02765125A patent/EP1436420B1/en not_active Expired - Lifetime
- 2002-10-03 AT AT02765125T patent/ATE360708T1/de active
- 2002-10-03 US US10/496,735 patent/US20050202427A1/en not_active Abandoned
- 2002-10-03 BR BR0206145-7A patent/BR0206145A/pt not_active IP Right Cessation
- 2002-10-03 AU AU2002329496A patent/AU2002329496B2/en not_active Ceased
- 2002-10-03 DK DK02765125T patent/DK1436420T3/da active
- 2002-10-03 DE DE60219813T patent/DE60219813T2/de not_active Expired - Lifetime
-
2003
- 2003-06-13 NO NO20032697A patent/NO20032697L/no not_active Application Discontinuation
-
2007
- 2007-07-13 CY CY20071100930T patent/CY1106724T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO20032697L (no) | 2003-07-15 |
| DK1436420T3 (da) | 2007-09-10 |
| EP1436420B1 (en) | 2007-04-25 |
| US20050202427A1 (en) | 2005-09-15 |
| GR1003966B (el) | 2002-08-06 |
| DE60219813T2 (de) | 2008-01-31 |
| NO20032697D0 (no) | 2003-06-13 |
| EP1436420A2 (en) | 2004-07-14 |
| BR0206145A (pt) | 2004-01-13 |
| AU2002329496B2 (en) | 2009-10-22 |
| PT1436420E (pt) | 2007-09-12 |
| ATE360708T1 (de) | 2007-05-15 |
| CA2468802A1 (en) | 2003-04-24 |
| ES2286276T3 (es) | 2007-12-01 |
| WO2003033735A2 (en) | 2003-04-24 |
| DE60219813D1 (de) | 2007-06-06 |
| YU48903A (sh) | 2006-08-17 |
| WO2003033735A3 (en) | 2003-09-12 |
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