CU20190090A7 - Método para remoción de impurezas de preparaciones basadas en polisacáridos capsulares bacterianos - Google Patents

Método para remoción de impurezas de preparaciones basadas en polisacáridos capsulares bacterianos

Info

Publication number
CU20190090A7
CU20190090A7 CU2019000090A CU20190090A CU20190090A7 CU 20190090 A7 CU20190090 A7 CU 20190090A7 CU 2019000090 A CU2019000090 A CU 2019000090A CU 20190090 A CU20190090 A CU 20190090A CU 20190090 A7 CU20190090 A7 CU 20190090A7
Authority
CU
Cuba
Prior art keywords
capsular polysaccharides
impurities
polysaccharides
bacterial
removal
Prior art date
Application number
CU2019000090A
Other languages
English (en)
Inventor
Dattatreya Sarma Annamraju
Rajeev Mhalasakant Dhere
Sambhaji Shankar Pisal
Original Assignee
Serum Institute Of India Pvt Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Serum Institute Of India Pvt Ltd filed Critical Serum Institute Of India Pvt Ltd
Publication of CU20190090A7 publication Critical patent/CU20190090A7/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/095—Neisseria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08H—DERIVATIVES OF NATURAL MACROMOLECULAR COMPOUNDS
    • C08H1/00—Macromolecular products derived from proteins
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/60—Medicinal preparations containing antigens or antibodies characteristics by the carrier linked to the antigen
    • A61K2039/6031—Proteins
    • A61K2039/6037—Bacterial toxins, e.g. diphteria toxoid [DT], tetanus toxoid [TT]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Materials Engineering (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Sustainable Development (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

<p>La presente invención se refiere a un proceso mejorado para purificación de polisacáridos capsulares bacterianos, más en específico polisacáridos capsulares de bacterias gramnegativas. El proceso comprende la concentración y diafiltración de recolección, tratamiento con detergente aniónico y álcali fuerte, seguido por centrifugación, diafiltración y precipitación con base en detergente catiónico de polisacáridos bacterianos. El proceso da por resultado la reducción significativa de impurezas de endotoxina, proteína y ácido nucleico, proporcionando de esta manera mayor recuperación de polisacárido capsular con los niveles deseados de O-acetilo. Este proceso es escalable, no enzimático y emplea menos pasos de purificación.</p>
CU2019000090A 2017-05-05 2018-05-03 Método para remoción de impurezas de preparaciones basadas en polisacáridos capsulares bacterianos CU20190090A7 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN201721015961 2017-05-05
PCT/IB2018/053069 WO2018203268A1 (en) 2017-05-05 2018-05-03 Method for removal of impurities from bacterial capsular polysaccharide based preparations

Publications (1)

Publication Number Publication Date
CU20190090A7 true CU20190090A7 (es) 2020-10-20

Family

ID=62705619

Family Applications (1)

Application Number Title Priority Date Filing Date
CU2019000090A CU20190090A7 (es) 2017-05-05 2018-05-03 Método para remoción de impurezas de preparaciones basadas en polisacáridos capsulares bacterianos

Country Status (17)

Country Link
US (2) US11739356B2 (es)
EP (1) EP3619241A1 (es)
JP (2) JP7319929B2 (es)
KR (1) KR102565771B1 (es)
CN (1) CN110831980B (es)
AU (1) AU2018262438B2 (es)
BR (1) BR112019022868A2 (es)
CA (1) CA3062399A1 (es)
CU (1) CU20190090A7 (es)
EA (1) EA201992636A1 (es)
GE (1) GEP20227408B (es)
MD (1) MD20190088A2 (es)
MX (1) MX2023013110A (es)
SA (1) SA519410488B1 (es)
UA (1) UA125937C2 (es)
WO (1) WO2018203268A1 (es)
ZA (1) ZA201908038B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI718144B (zh) * 2015-05-04 2021-02-11 美商輝瑞股份有限公司 Ｂ型鏈球菌多醣－蛋白質軛合物、製造軛合物之方法、含軛合物之免疫原性組成物、及其用途
CN111065388A (zh) * 2017-09-07 2020-04-24 默沙东公司 肺炎球菌多糖及其在免疫原性多糖-载体蛋白缀合物中的用途
JOP20200214A1 (ar) * 2019-09-03 2021-03-03 Serum Institute Of India Pvt Ltd تركيبات مولدة للمناعة ضد الأمراض المعوية وطرق لتحضيرها
KR102370468B1 (ko) * 2020-03-18 2022-03-03 재단법인대구경북과학기술원 생체 시료 내 잔존하는 음이온 계면 활성제와 이와 결합한 지질 또는 단백질 결합체, 및 마이셀을 제거하는 조성물, 방법 및 이의 용도
US20230183765A1 (en) * 2020-05-14 2023-06-15 Serum Institute Of India Private Limited Methods for simultaneous fragmentation and purification of bacterial polysaccharides
EP4232593A1 (en) * 2020-10-22 2023-08-30 Pfizer Inc. Methods for purifying bacterial polysaccharides
CN112521520A (zh) * 2020-12-04 2021-03-19 苏州微超生物科技有限公司 脑膜炎球菌荚膜多糖的制备方法
CN115561190B (zh) * 2022-12-01 2023-04-07 南京大学 一种分别定量细菌荚膜多糖和脂多糖的方法

Family Cites Families (14)

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US4686102A (en) 1984-04-12 1987-08-11 American Cyanamid Company Multivalent pneumococcal vaccine and preparation thereof
ATE278031T1 (de) * 1997-01-24 2004-10-15 Schweiz Serum & Impfinst Neues verfahren zur isolierung von polysacchariden
HUP0100623A3 (en) * 1997-12-23 2003-11-28 Baxter Healthcare S A Wallisel Procedures for the extraction and isolation of bacterial capsular polysaccharides for use as vaccines or linked to proteins as conjugates vaccines
GB0115176D0 (en) * 2001-06-20 2001-08-15 Chiron Spa Capular polysaccharide solubilisation and combination vaccines
GB0502096D0 (en) * 2005-02-01 2005-03-09 Chiron Srl Purification of streptococcal capsular polysaccharide
US7491517B2 (en) 2006-07-19 2009-02-17 Jeeri R Reddy Method of producing meningococcal meningitis vaccine for Neisseria meningitidis serotypes A,C,Y, and W-135
CN102653565A (zh) * 2011-03-03 2012-09-05 天津康希诺生物技术有限公司 一种从荚膜细菌发酵液纯化荚膜多糖的方法
RU2634405C2 (ru) * 2012-01-30 2017-10-26 Серум Инститьют Оф Индия Лтд. Иммуногенная композиция
CN102660603B (zh) * 2012-04-17 2015-03-25 江苏康泰生物医学技术有限公司 一种快速纯化细菌荚膜多糖的方法
CA2885625A1 (en) * 2012-10-02 2014-04-10 Glaxosmithkline Biologicals S.A. Nonlinear saccharide conjugates
CN104936615B (zh) * 2012-11-21 2017-03-29 印度血清研究所私人有限公司 高产量的细菌多糖的制备
MY186874A (en) * 2014-02-25 2021-08-26 Msd Wellcome Trust Hilleman Laboratories Pvt Ltd A novel downstream process for purifying polysaccharides
CN104592408A (zh) * 2015-01-11 2015-05-06 云南沃森生物技术股份有限公司 一种荚膜多糖的纯化方法
US10543266B2 (en) 2015-07-04 2020-01-28 Bharat Biotech International Ltd. Polysaccharide vaccine formulations and processes for industrial production of bacterial polysaccharides

Also Published As

Publication number Publication date
UA125937C2 (uk) 2022-07-13
JP2020518707A (ja) 2020-06-25
EP3619241A1 (en) 2020-03-11
GEP20227408B (en) 2022-08-25
US20200190549A1 (en) 2020-06-18
CA3062399A1 (en) 2018-11-08
CN110831980A (zh) 2020-02-21
EA201992636A1 (ru) 2020-04-17
AU2018262438A1 (en) 2020-01-02
CN110831980B (zh) 2022-09-27
WO2018203268A1 (en) 2018-11-08
ZA201908038B (en) 2021-06-30
SA519410488B1 (ar) 2023-12-21
US11739356B2 (en) 2023-08-29
MX2023013110A (es) 2023-11-17
US20240026405A1 (en) 2024-01-25
MD20190088A2 (ro) 2020-04-30
JP2023017929A (ja) 2023-02-07
AU2018262438B2 (en) 2022-07-14
BR112019022868A2 (pt) 2020-05-19
KR20200015524A (ko) 2020-02-12
KR102565771B1 (ko) 2023-08-10
JP7319929B2 (ja) 2023-08-02

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